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      • 천연약재로부터 문화재보존용 방충방균제 개발연구

        정용재,이규식,한성희,강대일,이명희,Chung, Yong-Jae,Lee, Kyu-Shik,Han, Sung-Hee,Kang, Dai-Ill,Lee, Myeong-Hui 국립문화재연구소 2001 保存科學硏究 Vol.22 No.-

        The germicidal and insecticidal properties of volatile components extracted from star anise(Illicium verum Hooker filius) and clove (Eugenia caryophyllata THUNBERG)were evaluated against five microorganisms and three insects for the purpose of developing biocidal active substances from medicinal plants. The volatile components of star anise and clove showed strong antimicrobial effect against Aspergillus niger, Penicillium funiculosum, Mucor hiemalis, Trichoderma viride, and Aureobasidium pullulans. The extracts of each medicine also showed insecticidal effects against Sitophilusoryzae L., Lyctus linearis GOZE, and Reticulitermes spertus kyushuensis Morimoto. Fumigant toxicities to adult insects were determined. In the case of fumiganttoxicity, the extract of star anise showed 100% mortality against R. spertus, S.oryzae, and L. linearis at rates of $2.5\mu\ell$, $50\mu\ell$, $250\mu\ell$/filter paper, respectively but showed no killing effects by clove. The volatile components of star anise and clove were investigated by means of GC/MS. The main constitute, anethole among 20components from star anise and eugenol among 9 components from clove were identified. The mixture of star anise and clove as the volume ratio of 2 : 1 showed higher properties for antimicrobial and insecticidal effect than each volatile component. A. niger was inhibited by the mixture(125ml/$m^3$) for up to 10 days of exposure. Also, from the result of observing state change of organic materials by volatile extracts of star anise and clove, volatile extracts effects have no effect on natural organic materials of organic cultural properties and can be used as biological control agent. As research contents as above, the insecticidal and germicidal agents from star anise and clove and the mixture of them were more efficient and high level to prevent biological damage for conservation of organic cultural properties. So they may be used in new development of biologicalinsecticidal and germicidal agents for conservation of cultural properties.

      • KCI등재

        오동나무상자의 항균활성 분석 및 활성 증진을 위한 천연 살생물제 적용연구

        정용재,강소영,최윤아,Chung, Yong-Jae,Kang, So-Yeong,Choi, Yun-A 한국문화재보존과학회 2008 보존과학회지 Vol.24 No.-

        고문서 보관을 위해 사용되고 있는 오동나무상자의 항균활성을 측정하기 위해 오동나무로부터 방출된 휘발성 물질에 대한 항균활성과 휘발성 유기물질의 주성분을 정성분석 하였다. 항균활성은 상자 내부에 TSA 배지를 장착한 미생물 포집기를 설치하고 포집 후 배지에 성장한 미생물의 집락수를 관찰하였다. 그 결과 실외에서 포집한 배지의 집락수 85개 대비 14.82%가 감소한 72개 집락수가 관찰되었다. 포집된 공기질을 열탈착시스템 및 가스크로마토그래피/질량분석법을 통해 분석한 결과, 오동나무 상자 내 VOCs에서 limonene이 특징적으로 검출되었다. Eugenol과 anethole을 주성분으로 하는 천연 살생물제 I과 II를 상자 내에 첨가하여 훈증에 의한 항균활성을 측정한 결과, 약제 모두 실외와 오동나무 상자 내부에서 포집된 미생물 개체수 대비 각각 92.6%, 99.9% 이상 감소율을 보였다. 위의 결과로부터 사용된 오동나무 상자로부터 방출된 휘발성 유기화합물에 의한 항균활성 및 그 유효성분은 분석되지 않았으며, 생물피해로부터 고서적을 장기보관하기 위한 유물 보관함의 효율을 증진시키기 위해 천연 살생물제의 적용 가능성을 확인할 수 있었다. In order to assess antifungal activity of a wooden storage box, which was made of Paulownia tomentosa and used for keeping ancient documents, antifungal activity of volatile organic compounds emitted from the box was investigated along with qualitative analysis on major substances of the compounds. After collecting floating microorganisms inside air tester, the fungal activity was assessed by counting the number of colonies growing on TSA media. Compared to the control which collected 85 colonies from outdoor, 72 colonies were observed showing reduction rate of 14.82%. Through GC/MS and TDS system analysis, limonene was detected from the volatile organic compounds as characteristic features. When the fungal activity was assessed through fumigation by adding natural biocide BI and BII containing eugenol and anethole as major substances, both biocides showed a strong fungal activity with respectively 92.6%(inside the box) and 99.9%(outdoor) of reduction rate. Although these results didn't clarify antifungal activity of the volatile organic compounds emitted from the Paulownia-wood storage box and their functional components, it was at least confirmed that there is application possibility of natural biocide to use for preservation of ancient documents with increased efficiency in controlling pests of wooden storage boxes.

      • 석조문화재의 생물학적 손상과 보존방안

        정용재,서민석,이규식,한성희,Chung, Yong-Jae,Seo, Min-Seok,Lee, Kyu-Shik,Han, Sung-Hee 국립문화재연구소 2003 保存科學硏究 Vol.24 No.-

        Stone has been one of the most intensely studied materials in conservation. Understanding the deterioration of stone needs various knowledge in different mineralogical and physical characteristics and its weathering response under different climate and environment. The alteration and weathering of stone is affected by natural or artificial elements whether they are physical, chemical or biological damaging factors. It can be said that the bio deterioration of stone is coupled with every environmental factors, which induce decomposition of stone structure, either directly or indirectly as a form of catalysis. Many elements contribute to the deterioration of stone monuments and other objects of cultural value such as pagoda, stature of Buddha, etc. This report concentrates on the action of biodeteriorative factorsincluding bacteria, algae and higher plants. Preventive and remedial methods and a selection of chemical treatments are also described.

      • 석조문화재 생물막 제거 및 처리방안 연구

        정용재,서민석,이규식,황진주,Chung, Yong-Jae,Seo, Min-Seok,Lee, Kyu-Shik,Hwang, Jin-Ju 국립문화재연구소 2005 保存科學硏究 Vol.26 No.-

        A consideration number of investigation have begun to elucidate the essential role biological agents play in the deterioration of stone. What is becoming clear is that many factors affect the durability of stone. Physical, chemical, and biological agentsact in co-association, ranging from synergistic to antagonistic, to deteriorate stone. Biodeterioration has usually been considered to be a degradation process following the initial deterioration effects of inorganic agents, especially objects of cultural value such as pagoda, stature of Buddha etc. These agents were thought to condition stone surfaces for microbial contamination due to structural changes and enrichment of inorganic organic nutrient substrates. This report concentrates on the action of biodeteriogens from bacteria to algae and higher plants. Preventive and remedial methods are surveyed, as are a selection of chemical treatments.

      • 종이 손상과 보존 기술에 대한 한·불 교류연구

        정용재,최정은,이규식,Chung, Yong-jae,Choi, Jung-eun,Lee, Kyu-shik 국립문화재연구소 2007 保存科學硏究 Vol.28 No.-

        This project aims to develop a partnership between France and Korea; the field of paper conservation with two mains objectives. The first object is to study the possibilities of implementing the use of traditional Korean paper in conservation workshops in France and Europe. The second one is to establish a long term collaborative research program to investigate the degradation mechanisms of ancient papers and particularly early waxed manuscripts founded in Korea and France which are now brittle and fallen into parts. This problem deserves an investigation using analytical tools such as SEC/MALS, GC/MS to understand the interactions between wax and cellulose during the ageing process and to recommend proper conservation treatment. A research plan could be establish for both institution in order to facilities an exchange of visiting scholars (scientist, post-doc) to implement and share knowledge and for carrying out the research.

      • KCI등재

        적의(翟衣) 유물의 색과 금사 분석

        정용재(Chung Yong Jae),유지아(Yu Ji A),정선혜(Jeong Seon Hye) 국립고궁박물관 2011 古宮文化 Vol.- No.4

        본 연구는 소장처 및 형태가 다른 적의 유물(국립고궁박물관 적의, 세종대학교 박물관 적의, 서울역사박물관 적의)에 대한 과학적 분석을 통해 금사의 성분과 색도를 분석하고, 각 3점의 과학적 분석 결과에 대한 비교 연구를 중심으로 하였다. 첫째, 적의 유물 부위별 금사의 형태적 분석으로 디지털 영상현미경을 이용하여 금사의 꼬임 횟수 및 너비 등을 분석하였다. 둘째, 휴대용 합금분석기(portable XRF)을 이용하여 적의에 사용된 금사 성분을 분석하였다. 셋째, 색도계를 이용하여 적의의 각 부분별색도 분석과 함께 적의 3점의 색도 비교를 통하여 정확한 색의 기록 및 비교연구를 실시하였다. 형태적 분석 결과 서울역사박물관 적의에서 금사의 꼬임 횟수 및 폭, 금박의 폭이 다른 2점과는 차이점을 보였고, XRF를 이용한 성분 분석 결과 고궁박물관 적의와 세종대 적의는 금사에서 금(Au)이 검출되어 금사로 확인되었으나, 서울역사박물관 적의의 경우 구리(Cu)가 검출되어 금속사로 확인되었다. 하피에 위치한 금박의 경우 전부 금이 검출되어 금박으로 확인되었으며, 고궁박물관 적의와 세종대 적의의 폐슬에 사용된 금사 역시 금이 검출되어 금사로 확인되었고 금속의 경우 은과 금의 합금(Au-Ag)으로 추정된다. 적의 유물 3점의 색 분석 결과, 적의 유물과 하피 유물의 청색 색도에서 서울역사박물관 적의·하피 유물의 청색 색도와 다른 2점의 청색과는 거의 다른 계열의 청색으로 분석되었고, 나머지 유물 및 색상에서는 거의 차이가 없어 같은 계열의 색상으로 분석되었다. 이러한 조사 결과를 바탕으로 하여 적의 유물 3점에 대한 금사의 형태적 통계 및 성분분석 통계, 색도 통계를 산출하고 비교함으로써 향후 금사의 과학적 규명 연구의 기초 자료로써 활용될 것으로 기대된다. This study makes a comparative study for three pieces of jeogui(collected in National Palace Museum of Korea, Sejong university museum, Seoul Museum of History) through componential, chromaticity analysis. First, we analyse a twist number, width of gold thread through a digital microscope to know each part of gold thread. Second, we use a portable XRF to analyse component of gold thread. Third, we use a color-difference meter to compare each parts of jeogui textile. As a result of analysis, there is a difference to twist, width of gold thread in other two jeogui’s. In jeogui’s which are owned National palace museum of Korea, Sejong university museum, XRF analysis can detect gold(Au). But in jeogui which is owned Seoul Museum of History, XRF analysis can detect copper(Cu). So we could know jeogui’s which are owned National palace museum of Korea, Sejong university museum is composed a gold thread, and we could know jeogui which is owned Seoul Museum of History is composed a metal fiber. gilt located in hapi is illuminated gilt because gold detected. Also pyeseul located in National Palace museum of Korea jeogui, Sejong university museum jeogui identified gold thread because gold detected. And metal is presumed gold-silver alloy. As a result of color difference analysis, there is a difference to blue color in jeogui, hapi of Seoul Museum of History. And there is a very little difference in other color. By computing formal, components, and chromaticity statistics of three pieces of jeogui’s gold thread and comparing domestic gold thread cases, the result of this research is expected to be utilized as a basic document for scientific investigation research of gold thread.

      • SCOPUSKCI등재

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