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박성보(Sung-Bo Park),김정도(Jeong-Do Kim),이나리(Na-Ri Lee),정진하(Jin-Ha Jeong),정성윤(Seong-Yun Jeong),이희섭(Hee-Seob Lee),황대연(Dae-Youn Hwang),이종섭(Jong-Sup Lee),손홍주(Hong-Joo Son) 한국생명과학회 2011 생명과학회지 Vol.21 No.10
본 연구에서는 ACE 저해능 및 항산화능이 있는 식물성 젖산균을 다양한 식물체로부터 분리한 후, 그 특성을 조사하였다. 김치, 부추, 포도 및 동동주에서 K-1, K-21, L-5, G-3 및 D-3 균주가 분리되었으며, 16S rRNA gene 염기서열 분석을 통하여 이들은 각각 Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum 및 L. brevis로 동정되었다. 분리균주들은 MRS broth에서 44.3-71.9%의 ACE 저해능을 나타내었으며, 특히 G-3, L-5, K-1 균주는 skim milk가 함유된 MRS broth에서 59.0-8-98.6%의 높은 ACE 저해능을 나타내었다. 분리균주는 42.5-82.7%의 DPPH radical 소거능을 나타내었으며, G-3 및 K-1 균주는 pH 2.5의 인공위액에서 42.2-88.1%의 높은 생존율을 나타내었다. 분리균주는 0.3% oxgall에서 24시간 배양시 55.4-112.8%의 내성을 나타내었다. 또한 분리균주는 유기산 생성에 따른 pH 감소 효과로 인하여 Pseudomonas aeruginosa를 포함한 일부 병원성 세균의 생육을 억제할 수 있었다. In this study, we isolated and characterized plant-associated lactic acid bacteria which are able to produce angiotensin-converting enzyme (ACE) inhibitory and antioxidative activities. Five lactic acid bacteria were isolated from plants (grape and leek), a plant-associated fermentative product (Kimchi) and Korean traditional alcohol (Dongdongju). Strains K-1 and K-21 from Kimchi, strain L-5 from leek, strain G-3 from grape, and strain D-3 from Dongdongju were identified as Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum, and L. brevis, respectively, by 16S rRNA gene analysis. ACE inhibitory activities of isolated strains ranged from 44.3 to 71.9% in the MRS broth. G-3, L-5 and K-1 strains especially showed high ACE inhibitory activities (59.8-98.69%) in the MRS broth containing skim milk. DPPH radical scavenging activities of the strains were in the range of 42.5-82.7%. All strains showed varying levels of resistance in artificial gastric fluid (pH 2.5), retaining viability ranging from 42.2 to 88.1% after 3 hr of incubation. All strains showed high resistance to 0.3% oxgall after 24 hr of incubation; survival rates were in the range of 55.4-112.8%. Isolated strains were found to be antagonistic to some pathogens including Pseudomonas aeruginosa.
수산폐기물 재활용을 위한 미더덕껍질 용매 추출물의 생리활성 탐색
이상미 ( Sang Mee Lee ),강은진 ( Eun Jin Kang ),고태훈 ( Tae Hun Go ),정성윤 ( Seong Yun Jeong ),박근태 ( Geun Tae Park ),이희섭 ( Hee Sup Lee ),황대연 ( Dae Youn Hwang ),정영진 ( Young Jin Jung ),손홍주 ( Hong Joo Son ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.1
Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and causes environmental problem. Although biological activities of S. clava were reported by many investigators, study on S. clava tunic was little. In this study, therefore, tyrosinase inhibition and antioxidative activities of extracts from S. clava tunic using different solvent were investigated for recycling of the fishery waste. Among extraction methods tested, autoclaved extraction (25.7%) and hot water extraction (18.2%) appeared to be effective for extraction. The highest total phenolic content was 46.6 mg/g in autoclaved extract while the highest flavonoid content was 23.0 mg/g in chloroform extract. All extracts possessed tyrosinase inhibition activity and the inhibition activity was concentration-dependent. Inhibition concentration (IC50) against tyrosinase activity was 0.36×104 mg/ml in ethanol extract, 0.11×103 mg/ml in acetone extract and 0.27 mg/ml in n-butanol extract. Among extracts tested, hot water and autoclaved extracts displayed higher antioxidative activity than organic solvent extracts. Therefore, our data suggest that extract from S. clava tunic may potential candidate for cosmetic product with whitening effect and medicine for diseases caused by various oxidative stresses.
김정도 ( Jeong Do Kim ),박성보 ( Sung Bo Park ),이나리 ( Na Ri Lee ),정진하 ( Jin Ha Jeong ),이희섭 ( Hee Seob Lee ),황대연 ( Dae Youn Hwang ),이종섭 ( Jong Sup Lee ),정성윤 ( Seong Yun Jeong ),손홍주 ( Hong Joo Son ) 한국미생물생명공학회(구 한국산업미생물학회) 2011 한국미생물·생명공학회지 Vol.39 No.3
Plant lactic acid bacteria were isolated from plant-associated fermentative foods and crops, and their probiotic properties were investigated. Isolates K27 and O2 were isolated from Kimchi and onion, and identified as Lactobacillus plantarum on the basis of 16S rRNA gene analysis. The two strains were highly resistant to acid (an MRS broth at pH 2.5), where the survival rates of L. plantarum K27 and L. plantarum O2 were 90.2% and 97.3%, respectively. L. plantarum K27 and L. plantarum O2 also showed high bile resistance to 0.5% oxgall, with a more than 70% survival rate. They showed an inhibitory effect against pathogenic strains of Escherichia coli KCCM 40880 and Pseudomonas aeruginosa ATCC 10145. The antibacterial effect of the two strains was probably due to the presence of lactic acid. ACE inhibitory activities of the two strains ranged from 72.8% to 80.6% in MRS broth. Notably, the two strains showed high ACE inhibitory activity (89.2~98.2%) in MRS broth containing 10% skim milk. Antioxidant activity was tested by DPPH radical scavenging activity, with antioxidant activities of the strains being in the range of 56.8~61.5%. The results obtained in this study suggest that L. plantarum K27 and L. plantarum O2 may be potential probiotic starter cultures with applications with fermentative products.
초산균에 의한 환경친화적 기능성소재인 세균 셀룰로오스 생산을 위한 정치배양조건 최적화
조광식 ( Kwang Sik Cho ),이상미 ( Sang Mee Lee ),정성윤 ( Seong Yun Jeong ),박근태 ( Geun Tae Park ),이희섭 ( Hee Sup Lee ),황대연 ( Dae Youn Hwang ),정영진 ( Young Jin Jung ),손홍주 ( Hong Joo Son ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.5
Bacterial cellulose (BC) has played important role as new functional material for food industry and industrial products based on its unique properties. The interest in BC from static cultures has increased steadily in recent years because of its potential for use in medicine and cosmetics. In this study, we investigated culture condition for BC production by Acetobacter sp. F15 in static culture. The strain F15, which was isolated from decayed fruit, was selected on the basis of BC thickness. The optimal medium compositions for BC production were glucose 7%, soytone 12%, K2HPO4 0.2%, NaH2PO4ㆍ2H2O 0.2%, lactic acid 0.05% and ethanol 0.3%, respectively. The strain F15 was able to produce BC at 26℃-36℃ with a maximum at 32 ℃. BC production occurred at pH 4.5-8 with a maximum at pH 6.5. Under these conditions, a maximum BC thickness of 12.15 mm was achieved after 9 days of cultivation; this value was about 2.3-fold higher than the thickness in basic medium. Scanning electron micrographs showed that BC from the optimal medium was more compact than plant cellulose and was reticulated structure consisting of ultrafine cellulose fibrils. BC from the optimal medium was found to be of cellulose type I, the same as typical native cellulose.
Bacillus subtilis D7에 의하여 발효된 백태 청국장의 특성
이나리(Na-Ri Lee),박성보(Sung-Bo Park),이상미(Sang-Mee Lee),고태훈(Tae-Hun Go),황대연(Dae-Youn Hwang),김동섭(Dong-Seob Kim),정성윤(Seong-Yun Jeong),손홍주(Hong-Joo Son) 한국생명과학회 2013 생명과학회지 Vol.23 No.4
본 연구에서는 Bacillus subtilis D7과 발아콩을 이용하여 발효된 청국장의 특성을 조사하였다. 백태는 6시간 침지 후, 25℃에서 2시간마다 4일 동안 물을 공급했을 때, 발아율이 가장 높았다. 총 이소플라본 함량은 발아 전 971.3 μg/g이었고, 발아 후 1023.8 μg/g이었다. 실험에 사용된 모든 청국장에서 아미노태 질소 및 암모니아태질소 함량은 배양시간이 경과함에 따라 증가하였고, 청국장의 pH는 발효과정을 통하여 pH 7.8-8.0로 증가하였다. 청국장의 생균수는 발효 24시간까지 급증한 후, 약간 감소하였다. 청국장의 protease 활성은 발효 30-36시간까지 증가하였고, B. subtilis D7를 이용한 청국장의 α- 및 β-amylase 활성은 발효 12-18시간까지 증가하였다. 청국장을 섭취한 마우스의 ALP 활성과 SOD 활성은 대조군보다 매우 높았다. AST 활성은 미발아콩 청국장 섭취군보다 발아콩 청국장 섭취군에서 더 높았다. 결론적으로 B. subtilis D7을 이용하여 조제된 발아콩 청국장은 품질 특성이 향상되었고, 기능성 성분의 함량이 증가되었다. This study was carried out to investigate the characteristics of white soybean Chungkookjang fermented by Bacillus subtilis D7. The highest germination rate was obtained under 25°C when water was supplied for 4 days at intervals of 2 hr. The total isoflavone content was 971.3 μg/g before germination and 1023.8 μg/g after germination. The amino type- and ammonia type-nitrogen contents of Chungkookjang were proportional to the fermentation time. The pH values of all Chungkookjang soybeans increased up to pH 7.8-8.0 during fermentation. The number of viable cells in all Chungkookjang soybeans increased significantly up to 24 hr. The protease activity of all Chungkookjang soybeans increased up to 30-36 hr. The α- and β-amylase activities of Chungkookjang fermented by B. subtilis D7 were enhanced with increasing fermentation time. Analysis of the effect of Chungkookjang intake on the liver function of mouse showed that the alkaline phosphatase (ALP) activity and the superoxide dismutase (SOD) activity in the Chungkookjang diet group were markedly higher than those in the control group. The asparatate aminotransferase (AST) activity in the germinated soybean Chungkookjang diet group was higher than that in the nongerminated soybean Chungkookjang diet group. Therefore, Chungkookjang soybeans fermented with B. subtilis D7 can be expected to have an increased content of functional components and improved quality characteristics.
학교 강의실 조명에 대한 에너지 절약과 균제도에 관한 연구
최홍규(Hong-kyoo Choi),최병숙(Byoung-sook Choi),조경남(Kyung-nam Cho),조계술(Kyeh-sul Cho),김정한(Jung-han Kim),김성수(Sung-su Kim),조의상(Eui-sang Cho),정성윤(Sung-Youn Jeong) 한국조명·전기설비학회 2000 한국조명·전기설비학회 학술대회논문집 Vol.2000 No.-
본 연구는 교육 환경 중 조명의 역할은 학생들의 시각작업과 학습효과의 향상 및 심신 형성 등에 매우 중요하며, 실내에서 생활하는 시간이 많아지므로 일정 수준이상의 조도가 필요하다. 적당한 조도와 휘도 분포를 적절하게 조절하면 눈의 피로를 줄이고, 휴식의 효과로 학습능률의 효율성을 높일 수 있다. 현재 수업중인 2개교를 선택하여 조도를 측정하고, 컴퓨터 시뮬레이션 방법을 이용하여 조도분포에 따른 균제도와 휘도를 개선하여 경제성을 향상시키고 에너지 절약을 가져올 수 있다.