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        간세포암 환자에서 DNase , RNase 및 Rnase inhibitor 측정의 의의

        계세협(Sea Hyub Kae),정유선(Yoo Sun Chung),장현주(Heon Ju Jang),정선화(Sun Wha Jung),김용태(Yong Tae Kim),강승식(Seung Sik Kang),이진(Jin Lee),곽상택(Sang Taek Kwak),주상언(Sang Aun Joo),유재영(Jae Young Yoo) 대한내과학회 1998 대한내과학회지 Vol.54 No.5

        N/A Objective Activities of nucleases (acid DNase and neutral RNase) and RNase inhibitor 1mown to be involved in carcinogenesis and suppression of cancer were determined in cancer tissue, serum and ascitic fluid of patients with hepatocellular carcinoma and were compared with those of the controls. Also studied were nucleases and RNase inhibitor isolated from hepatocellular carcinoma tissue and ascitic fluid of the cancer patients to evaluate the properties and interactions between them. Method: Activities of nucleases and RNase inhibitor were measured in cancer tissue, serum and ascitic fluid of patients with hepatocellular carcinoma by ultraviolet spectrophotometry. Nucleases and RNase inhibitor were isolated from hepatocellular carcinoma tissue and ascitic fluid of the cancer patients by DEAE-cellulose column chromatography. As controls, normal tissue of the cancer patients, serum of healthy persons and ascitic fluid of cirrhotic patients were used. Result: Activities of DNase, RNase and RNase inhibitor were significantly increased in hepatocellular carcinoma tissue. DNase activity was not detected, RNase activity was increased and RNase inhibitor activity was unchanged in both serum and ascitic fluid of the hepatocellular carcinoma patients. DNase was isolated as a single enzyme and RNase as seven isozymes from the hepatocellular carcinoma tissue. The DNase isolated preferentially cleaved ds DNA over ss DNA and was endonuclease in nature (majority of hydrolytic products of DNA by the DNase were oligodeoxyribonucleotides). Of seven RNase isozymes isolated from the hepatocellular carcinoma tissue, isozyme I exhibited nonsecretory nature of RNase and other six isozymes secretory nature of the enzyme. Activity of RNase isozyme V was greatly increased and the activity of inhibitor complexed with the isozyme V was also increased. RNase in ascitic fluid of the cancer patient was separated into four isozymes, of which isozyme I exhibited mixed form of secretory and nonseretory nature and greatly increased in its activity. RNase isozyme V isolated in the hepatocellular carcinoma tissue was not detected in the ascitic fluid, Conclusion: The use of the nucleases and the inhibitor in the cancer tissue as biochemical markers for the hepatocellular carcinoma was suggested. RNase was released into the body fluid from the cancer tissue and could be used as a diagnostic marker for the hepatocellular carcinoma. An important rale of the DNase in carcinogenesis of the liver was suggested. RNase isozyme V was limited in the cancer tissue and RNase isozyme I and V and inhibitors associated with these isozymes might be involved in carcinogenesis processes, suppression of cancer and maintenance of tocellular carcinoma through their interactions.

      • 2007년도 덕성여자대학교 신입생의 특성

        정형남,정선화,김남재 덕성여자대학 학생생활지도연구소 2007 學生生活硏究 Vol.23 No.-

        2007년도 덕성여자대학교 신입생 및 그 가정의 제반특성을 파악하기 위하여 인적 사항, 가정환경, 입학 만족도, 대학생활, 가치관, 자신에 대한 지각, 강의주제 등에 관한 설문조사를 실시하였다. 총 신입생 1281명 중 응답자 1066명의 자료를 분석하여 각 문항 별로 신입생 전체는 물론 각 학부의 응답 빈도 및 비율을 제시하였다. 되풀이 확인된 결과로는 우선, 대다수의 학생들이 수도권지역의 고등학교를 졸업하였으며 중산층 이상의 가정 출신이라는 점이다. 대부분 부모가 모두 생존해 계시고 고졸이상의 학력을 가지고 있다. 두번째, 학생들은 취업에 큰 관심을 가지고 있으며 장래 취업을 준비하기 위해 대학에 진학 했다고 답한 경우가 많았다. 이외에도 대학별 특징 등 중요한 결과들을 논의에서 요약하였다. 그리고 그 결과들이 시사하는 바를 제안하였다. Examined the characteristics of the students who entered Duksung Women's University in 2007. The total number of 1066(out of 1281) undergraduates completed an autobiographical questionnaire which inquires about demographic variables, family environment, satisfaction with the university, expectancies of a campus life, value system, self-concept, and lecture subject. Results show that a majority of the students were graduated from high schools located in Seoul. Both of their parents are alive, in the middle or higher socioeconomic groups and of high educational accomplishment(at the lowest, high school graduates). One of the replicated findings is that students are highly interested in career development. A great proportion of career preparation. The result also indicate that many students have chosen our university solely based on their grades. And many students report that they are satisfied with their choice, which is very encouraging. Other result such as the characteristics unique to each college are also summarized and several suggestions are presented In the discussion section.

      • 2008년도 덕성여자대학교 신입생의 특성

        정선화,김남재 덕성여자대학 학생생활지도연구소 2008 學生生活硏究 Vol.24 No.-

        Examined the characteristics of the students who entered Duksung Women's University in 2008. The total number of 1054(out of 1445) undergraduates completed an autobiographical questionnaire which inquires about demographic variables, family environment, satisfaction with the university, expectancies of a campus life, value system, self-concept, and lecture subject. Results show that a majority of the students were graduated from high schools located in Seoul. Both of their parents are alive, in the middle or higher socioeconomic groups and of high educational accomplishment(at the lowest, high school graduates). One of the replicated findings is that students are highly interested in career development. A great proportion of career preparation. The result also indicate that many students have chosen our university solely based on their grades. And many students report that they are satisfied with their choice, which is very encouraging. Other result such as the characteristics unique to each college are also summarized and several suggestions are presented in the discussion section.

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