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Evaluation of Saponin Loaded Gellan Gum Hydrogel Scaffold for Cartilage Regeneration
전하얀,신은영,최주희,송정은,Rui L. Reis,강길선 한국고분자학회 2018 Macromolecular Research Vol.26 No.8
Several concentrations of saponin (Sa), which is water-soluble, were fabricated well with gellan gum (GG) solution. Sa, found in many plants like clematis and ginseng, has long been used in traditional chinese medicine to treat joint diseases including osteoarthritis. Also it has its ability of anti-inflammatory, antioxidant and anti-cancer. GG hydrogel is suitable as a cell encapsulating agent in the field of cartilage regeneration because of its easy processing and biocompatibility. In this study, GG hydrogel scaffolds with different concentration of Sa were conducted for analysis by SEM, FT-IR, compressive strength, water uptake, degradation rate, MTT assay, mRNA expression. It was observed that 0.025wt% Sa/GG hydrogel scaffold shows good morphology, cell proliferation and mRNA expression results. The composite material supports cell growth covered with extracellular matrix (ECM) with maintaining its function. As a result, incorporation of Sa loaded with GG hydrogel scaffolds had positive result up to 0.025 wt% in cartilage regeneration.
여러 가지 천연고분자를 함유한 PLGA 하이브리드 지지체에서 토끼 골수줄기세포의 골분화 능력 평가
전하얀(Ha Yan Jeon),김수민(Soo Min Kim),전유신(Yoo Shin Jeon),국연지(Yeon Ji Kook),송정은(Jeong Eun Song),강길선(Gilson Khang) 한국고분자학회 2017 폴리머 Vol.41 No.5
합성고분자인 poly(lactic-co-glycolic acid)(PLGA)는 우수한 생분해능을 가진 생체재료로서 널리 이용되고 있으며, 천연유래 고분자인 실크와 소장 점막하 조직, 탈 미네랄화 골분 그리고 오리발 유래의 콜라겐은 생체 적합한 특성을 가지고 있다. 본 논문에서는 여러 가지 천연유래 고분자를 함유한 PLGA를 제작한 후, 지지체의 물성 및 표면을 확인하였으며 토끼 골수유래줄기세포를 이용하여 지지체에 파종 후, 세포의 생존율과 세포분화 정도를 측정하였고 in vivo 실험에서 면역조직학적 염색인 H&E와 Von Kossa을 실시하여 골분화 정도를 확인하였다. 결과적으로 오리발 유래 콜라겐과 소장 점막하 조직을 함유한 지지체에서 빠른 골 분화의 모습을 확인할 수 있었다. Poly(lactic-co-glycolic acid) (PLGA) has been most widely used owing to its advantages such as good biodegradability and biocompatibility. Scaffolds made of natural source bio-materials like small intestinal submucosa (SIS), demineralized bone particles (DBP), silk fibroin (SF), and collagen-derived duck’s feet collagen (DC) have been proved to be biocompatible. We manufactured composite scaffolds of all these natural biomaterials/PLGA. After making hybrid scaffolds, we measured mechanical strength of scaffolds and observed a porosity and surface morphology of the scaffolds by scanning electronic microscopy. Rabbit bone marrow mesenchymal stem cells (rBMSCs) were cultured, expanded and seeded on scaffolds. Inflammatory responses were checked at 1, 4 and 8 weeks using H&E & Von Kossa staining. DC and SIS group were found to be faster in bone regeneration than other groups. Finally, we have observed that scaffolds using biomaterials are effective in cell attachment, stem cell differentiation, and mineralized tissue formation.
실크 피브로인과 젤란검 하이드로젤에서 연골 재생 효과에 대한 연구
박종호,전하얀,전유신,박현,김초민,송정은,강길선 한국고분자학회 2018 폴리머 Vol.42 No.2
최근 조직 공학에서 새로운 생체 재료로 관심을 받고 있는 젤란검은 포도당, 글루콘산, 람노스로 구성된 다당류로서 연골 조직 재생의 대체재로 각광받고 있다. 실크 피브로인은 생체적합성과 생분해성이 뛰어나며 우수한 기계적 특성을 갖고 있다. 본 실험은 실크 피브로인이 연골 재생에 미치는 영향을 확인하기 위해 2 wt% 젤란검 수용액에 2 wt% 실크 피브로인 수용액을 100/0, 70/50, 50/50 비율로 첨가하여 하이드로젤 지지체를 제조하였으며, 실크 피브로인 수용액이 다양한 함량으로 제작된 젤란검 하이드로젤 지지체의 물리적 특성을 알아보고자 압축강도, 다공도, FTIR 등을 실시하였고 연골 세포의 증식과 유전자 발현을 평가하기 위해 MTT, bio-SEM, RT-PCR을 실시한 결과 50/50 비율의 젤란검/실크 피브로인(GG/SF) 하이드로젤 지지체에서 높은 세포 증식률과 우수한 연골재생효과를 확인하였다. 결과적으로 50/50 비율의 젤란검/실크 피브로인 하이드로젤 지지체가 연골 조직 재생에 긍정적으로 작용함을 확인하였다.
송정은,전하얀,최주희,이대훈,강길선 한국고분자학회 2022 Macromolecular Research Vol.30 No.4
Corneal endothelial cells (CEnCs) cannot be regenerated once damaged, so restoring corneal function can only be achieved through tissue engineering. In this study, biocompatible films were prepared from silk fibroin (SF) and taurine (Ta) as a carrier for the regeneration of CEnCs. Ta/SF films were prepared by mixing Ta at various concentrations (0, 0.25, 0.5, 1, and 2 mM) in a 7% SF solution to determine the effects of Ta concentrations on cornea endothelial proliferation. We observed that the 0.25 mM Ta/SF film produced the highest number of viable cells and cellular proliferation. Ta/SF film was also found to maintain cell morphology and cell functionality. Current research on graft materials for corneal regeneration focuses on maintaining transparency, adhesion, and proliferation of corneal endothelial cells. In addition to the above-mentioned advantages, the Ta/SF film in this study creates an environment similar to the native CEnCs environment by using Ta, an amino acid abundantly present in the eye tissue. Therefore, Ta/SF film may be useful as a new treatment modality and a carrier for CEnCs in cornea tissue regeneration.