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전종용,이건형 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
This fluorescent chemosensor designed for detecting Hg(II) in aqueous solutions based on the fluorescence quenching process. The sensor includes tryptophan that has unique function as a ligand as well as a quencher for recognition and fluorescent change by a metal binding event. The fluorescent change is occurred by photoinduced electron transfer (PET) process. ABD-W showed sensitive and selective fluorescent turn-on response to Hg(II) among several metal ions. The binding mode of sensor with Hg(II) ions was proposed on the basis of the results of pH titration experiment, and ESI-mass spectrometry. We are planning to further study about this ABD-W sensor.
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전종용,이건형 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
We synthesized a fluorescent peptidyl chemosensor based on ArsR regulator peptide sequence for sensitive detection of heavy metal ions. Interestingly, the chemosensor showed responses to 16 metal ions in aqueous buffered solutions, whereas the chemosensor in the presence of EDTA, a chelating agent, showed an exclusively selective response to Cu(II) among tested metal ions. Upon addition of Cu(II), the chemosensor in the presence of EDTA exhibited a ratiometric response to Cu(II) by increasing pyrene excimer emission and decreasing pyrene monomer emissions. We investigated the binding mode of the chemosensor with Cu(II) in the presence of EDTA by using mass spectrometer, pH titration, and UV absorbance. According to our results, the Cys residue in the peptide receptor part of the chemosensor play a critical role for the recognition of Cu(II) in the presence of EDTA.
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전종용,이건형 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Although the metal binding ability of tryptophan is well known, it is relatively less known that it has unique quenching ability to fluorophore. We synthesized a fluorescent probe based on the natural amino acid with 4-dimethylamine-7-sulfonyl-2,1,3-benzoxadiazole fluorophore. This fluorescent chemosensor designed for detecting Hg(II) in aqueous solutions using dual role of tryptophan at higher wavelength. The probe includes tryptophan that acts as a ligand for heavy metal ions, especially mercury ion in this case, and quencher for fluorophore by photoinduced electron transfer (PET) process. In aqueous buffered solution, the probe showed highly selective turn-on response to mercury ion among 16 other metal ions. After the probe bound with mercury ion, it exhibited sensitive turn-on fluorescent response and color change with red shift. The cell penetration test conducted with ABD-W and it can measure concentration of mercury ion in the live cells effectively.
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질식초음파를 이용한 자궁벽을 통한 태반 융모막 융모 채취법 ; 경질적 경근적 융모막 융모 채취법
정환욱,오기석,강인수,최수경,황정혜,이종표,김재영,전종용 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.1
1991년 1월 1일부터 1992년 6월 30일까지 본원에 내원한 환자중 산전유전질환의 진단이 필요한 산모 87예를 대상으로, 질식초음파 감시하에 천자침으로 자궁벽을 통하여 융모막 융모 생검으 실시한 결과 다음과 같은 성적을 얻었다. 1. 대상 87예의 적응증증 모성연령증가가 50예(57.7%)로 가장 많았으며, 평균 연령은 35세였고, 평균 임신주수는 10주였다. 2. 천자침의 삽입횟수는 평균 1.2회였고, 3회이상 삽입하지는 않았다. 3. 융모막 융모 채취성공율은 98.8%로, 총대상 87예중 86예에서 채취가 가능하였고, 80명에서 1회 천자로 채취가 가능하여 91.9%의 성공율을 보였다. 4. 채취된 융모양은 5-20 mg 으로 평균 14 mg이었고, 대상 모두에서 검사하기에 충분한 양이었다. 5. 염색체이상 발생빈도는 8%로 7예에서 염색체 이상을 보였다. 6. 합병증으로는 융모막하혈종이 13.8%로 발생하였으나, 대부분 1주후 소실되었고, 기타 질출혈 2회, 자연유산 1예였으나 감염 및 양수과소증 등의 다른 합병증은 없었다. 결론적으로 질식초음파를 이용한 융모막 융모채취법은 감염의 위험성이 적고 실시하기에 용이하며 안전하므로 기존의 고식적인 자궁경부를 통하거나 복벽을 통과하는 방법보다 유용한 방법으로 사료된다. Data are presented on 87 patients undergoing chorionic villus sampling procedures by new sampling technique: Transvaginal transmural catheter aspiration method undertransvaginal ultrasound guided at Cheil General Hospital from January, 1991 to June 1992. Eighty seven pregnant women underwent Transvaginal-Transmural Chorionic villus sampling(TM-CVS) between 8 and 10 weeks of gestation. Sampling was usually carried out at ninth or tenth weeks of gestation (56 cases, 64.3%). Transmural chorionic villus sampling had an overall success rate of 98.8%, with only one insertion of the guide needle required for 91.9% of patients. Analysis of the 87 samples revealed seven anomalities *8%). The spontaneous abortion rate for these method was 1.1% (1.87). Of 87 attemped cases, subchorionic hematoma occured in 12 cases (13.9%) and vaginal bleeding in 2 cases. These results demonstrate that the transvaginal transmural procedure can be rapidly and effectively incorporated by and operator already experienced with transcervical and transabdominal chorionic villus sampling.
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