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저염분 바이오플락에서 사육한 흰다리새우(Litopenaeus vannamei)의 해수 순치방법에 따른 혈장성분 및 혈장삼투압 비교
전유현 ( Yu-hyeon Jeon ),이종민 ( Jong-min Lee ),김수경 ( Su Kyung Kim ),김수경(2) ( Su-kyoung Kim(2) ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.5
This study was carried out to identify an effective method to acclimate low-salinity (4 psu) bred Litopenaeus van-namei (mean body weight 16±3.3 g) to sea water. The fast acclimation group (FA) was directly exposed to filtered sea water (32 psu) while the slow acclimation group (SA) was exposed to a slow increase in salinity. Shrimps were sampled at 0, 1, 3, 6, 12, 24 and 48 h for plasma analyses. The plasma components between experimental groups did not show significant differences. The hemolymph osmolality (HO) in FA increased significantly after 1 h (P<0.05), while in SA it started to increase slowly only at 24 h and reached a similar level to that of FA at 48 h. The levels of Na+ and Mg<sup>2+</sup> ions were significantly different between the two treatments (P<0.05), but there was no significant dif-ference in Ca<sup>2+</sup> ion levels. We found that the different methods of acclimation of L. vannamei to sea water do not af-fect the plasma components significantly, but lead to changes in the HO and ion levels, it is considerable to acclimate gradually for at least two days.
넙치, Paralichthys olivaceus의 미세플라스틱 급성 노출에 따른 혈액성상 및 혈장성분의 변화
김소희,김가현,김지수,김준환,전유현,조재황,김석렬,김대희,Kim, So-Hee,Kim, Ga-Hyun,Kim, Ji-Su,Kim, Jun-Hwan,Jeon, Yu-Hyeon,Cho, Jae-Hwang,Kim, Seok-Ryel,Kim, Dae-Hee 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.3
Juvenile olive flounder, Paralichthys olivaceus (mean weight 66.7±7.1 g; mean length 19.2±0.9 cm) in a bio-floc environment were exposed to microplastic (PE: polyethylene, size 40-48 ㎛) at 0, 4, 20, 100, 500 and 2,500 mg L<sup>-1</sup> for 96 hours. No P. olivaceus deaths were observed following microplastic exposure. In the plasma components, calcium was significantly decreased whereas there was no significant change with magnesium following microplastic exposure. Glucose was significantly decreased with over 100mg L<sup>-1</sup> at 48 hours and 20mg L<sup>-1</sup> at 96 hours. Cholesterol was significantly decreased with over 20mg L<sup>-1</sup> after 48 hours, whereas there was no significant change in the total protein content. In enzymatic plasma components, the AST(Aspartate aminotransferase) was significant decreased by microplastic exposure. The results of this study indicate that acute exposure to microplastic induces blood physiological changes in P. olivaceus. 본 실험에서 미세플라스틱의 노출은 넙치의 혈액학적 성상인 hemoglobin 및 hematocrit 수치의 유의적인 감소를 유발하였다. 또한 미세플라스틱의 노출은 넙치 혈장 무기성분인 calcium, 유기성분인 glucose 및 cholesterol, 효소성분인 AST의 유의적인 변화를 나타냈다. 본 연구에서 바이오플락 환경에서 미세플라스틱의 노출이 어류의 혈액생리에 영향을 주며 독성으로 작용하는 것을 확인할 수 있었다. 향후, 일반 해수와 바이오플락 환경으로 각각 양성한 넙치를 이용하여 사육환경의 변화에 의한 미세플라스틱 노출영향의 차이에 대해서도 추가적인 연구가 이루어져야 할 것이다. 본 연구는 다양한 어종에서 종별 미세플라스틱의 농도 연구에서 미세플라스틱 노출에 따른 종별 독성영향비교평가를 위한 기본 자료로 활용될 수 있을 것이다. 하지만, 바이오플락 환경에서 미세플라스틱의 응집은 미세플라스틱의 독성에 영향을 미치는 중요한 요소이기 때문에(Choi et al. 2020), 향후 연구에서 철저한 모니터링과 함께 다양한 미세플라스틱 독성에 미치는 요소에 대한 추가연구가 필요할 것이다.
수온별 아질산 급성 노출에 따른 넙치, Paralichthys olivaceus의 혈액학적 성상 및 혈장성분의 영향
홍수민 ( Su-min Hong ),조아현 ( A-hyun Jo ),김다은 ( Da-eun Kim ),박연숙 ( Yeon-sook Park ),이혜성 ( Hye-sung Lee ),전유현 ( Yu-hyeon Jeon ),김석렬 ( Seok-ryel Kim ),김대희 ( Dae-hee Kim ),강예재 ( Yue Jai Kang ),김준환 ( Jun-hwan 한국어병학회 2021 한국어병학회지 Vol.34 No.2
Olive flounder (Paralichthys olivaceus) (Weight 110.9±17.1 g, length 22.3±1.2 cm) were exposed to waterborne nitrite at 0, 30, 60, 120, 240, 480 and 960 mg NO<sub>2</sub> <sup>-</sup>/L according to water temperature at 20℃ and 25℃ for 96 hours. The lethal concentration 50 (LC<sub>50</sub>) of olive flounder, P. olivaceus exposed to waterborne nitrite was 513.87 mg NO<sub>2</sub> <sup>-</sup>/L at 20℃ and 208.35 mg NO<sub>2</sub> <sup>-</sup>/L at 25℃, which means a significant difference in LC<sub>50</sub> by the water temperature. Hemoglobin and hematocrit were significantly decreased by waterborne nitrite exposure. The inorganic component, plasma calcium, was significantly decreased, and the organic components such as plasma glucose and cholesterol were significantly decreased showing a similar tendency with calcium. In enzymatic components, the AST and ALP were also significantly decreased by nitrite exposure. The results of this study indicate that exposure to nitrite can affect the survival and hematological physiology of P. olivaceus, and the effect of exposure to nitrite had a significant effect on nitrite toxicity depending on the water temperature.