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수온별 아질산 급성 노출에 따른 넙치, Paralichthys olivaceus의 혈액학적 성상 및 혈장성분의 영향
홍수민 ( Su-min Hong ),조아현 ( A-hyun Jo ),김다은 ( Da-eun Kim ),박연숙 ( Yeon-sook Park ),이혜성 ( Hye-sung Lee ),전유현 ( Yu-hyeon Jeon ),김석렬 ( Seok-ryel Kim ),김대희 ( Dae-hee Kim ),강예재 ( Yue Jai Kang ),김준환 ( Jun-hwan 한국어병학회 2021 한국어병학회지 Vol.34 No.2
Olive flounder (Paralichthys olivaceus) (Weight 110.9±17.1 g, length 22.3±1.2 cm) were exposed to waterborne nitrite at 0, 30, 60, 120, 240, 480 and 960 mg NO<sub>2</sub> <sup>-</sup>/L according to water temperature at 20℃ and 25℃ for 96 hours. The lethal concentration 50 (LC<sub>50</sub>) of olive flounder, P. olivaceus exposed to waterborne nitrite was 513.87 mg NO<sub>2</sub> <sup>-</sup>/L at 20℃ and 208.35 mg NO<sub>2</sub> <sup>-</sup>/L at 25℃, which means a significant difference in LC<sub>50</sub> by the water temperature. Hemoglobin and hematocrit were significantly decreased by waterborne nitrite exposure. The inorganic component, plasma calcium, was significantly decreased, and the organic components such as plasma glucose and cholesterol were significantly decreased showing a similar tendency with calcium. In enzymatic components, the AST and ALP were also significantly decreased by nitrite exposure. The results of this study indicate that exposure to nitrite can affect the survival and hematological physiology of P. olivaceus, and the effect of exposure to nitrite had a significant effect on nitrite toxicity depending on the water temperature.