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주반멘,장유선,이은주,이은실,강종성 충남대학교 약학대학 의약품개발연구소 2010 藥學論文集 Vol.25 No.-
Lonicerae Folium et Caulis is a commonly used anti-inflammatory herbal drug. The therapeuticeffectiveness of this drug depends significantly on the geographical origin, hence, the standardization and the geographical authenticity verification using the chemical characteristics would be very important. In the present study, an HPLC method for simple and effective analysis of four main components like coniferin, loganic acid, sweroside and loganin from the Lonicerae Folium et Caulis sample was developed and validated. Four main components were base line separated on a Optimapak C18 column with linear gradient of mobile phase A(10% methanol containing 0.1% formic acid) and B(90% methanol containing 0.1% formic acid) for 0 min 0%B, 15 min 30%B, 25 min 70%B, 30 min 70%B, 35 min 100%B. The flow rate was 1.0 mL/min and detection was carried out at 254 nm. Methylparaben was used as internal standard. Pattern recognition analysis using similarity index and principal components analysis revealed that Korean samples could not discriminated from Chinese samples because the contents of the marker components of samples were very similar. This method was validated according to ICH Guidelines and the method was found to be simple, accurate, precise, economical, and robust.
키랄이동상 HPLC를 이용한 시판 나프록센정 중의 나프록센 광학이성질체의 분석
홍정희,채규한,이은실,이은주,김태진,김상혁,장유선,강종성 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-
The naproxen enantiomers were separated and analyzed by HPLC using ordinary reversed column and chiral mobile phase additive. The methods were validated with the terms of linearity, intraday and interday precision, accuracy and selectivity. The (R)- and (S)-naproxen were successfully separated on Zorbax SB-C18 column with a mixture of 0.05 M phosphate buffer (pH 3) containing 10 mM methyl-β -cyclodextrin as a chiral selector and methanol (80:20, v/v) as mobile phase. The flow rate was 0.2 mL/min and naproxen was detected with UV at 231 nm. The range of intra- and inter-day prescision of (S)-naproxen were 1.05~1.62% and 1.05~2.96%, respectively, with accuracy of 99.6~101.1%. The contents of (S)-naproxen in commercial naproxen tablets were determined by validated method and showed 93.2~101.1% of stated amounts.