바코딩 기법을 활용한 참조기(Larimichthys polyactis) 산란장에 대한 새로운 조명

장서하,김진구 한국수산과학회 양식분과 2020 한국수산과학회 양식분과 학술대회 Vol.2020 No.7

2018-2020년 한국 주변해역에서 채집된 날개멸속(Bregmaceros) 어류 자치어 3종의 분자동정 및 형태기재

장서하,김진구,유효재 한국수산과학회 양식분과 2020 한국수산과학회 양식분과 학술대회 Vol.2020 No.7

한국 제주도 근해에서 출현한 황성대과(Peristediidae) 어류 1미기록종, Peristedion liorhynchus 치어의 형태기재 및 분자동정

장서하,김진구,유효재 한국수산과학회 2020 한국수산과학회지 Vol.53 No.5

In April 2019, a single Peristediidae juvenile (14.1 mm SL) was collected from the waters off of Jeju-do Island, Korea. DNA barcoding identified the juvenile as Peristedion liorhynchus, an unrecorded species in Korea. P. liorhynchus has eight dorsal fin spines, 22 dorsal and 19 anal fin rays, and a long third pectoral fin ray that passes through the middle of the tail at the juvenile stage. Juvenile also have large heads, extensive head spination, and serrated edges on the ocular and parietal spines. This is the first record of P. liorhynchus in Korea; therefore, we propose the new Korean name, “Nam-bang-hwang-seong-dae”.

갈가자미, Tanakius kitaharae와 기름가자미, Glyptocephalus stelleri (가자미과) 자어의 형태 비교

장서하,정경미,박정호,김진구 한국어류학회 2016 韓國魚類學會誌 Vol.28 No.3

Tanakius kitaharae and Glyptocephalus stelleri, belonging to the family Pleuronectidae, were very similar in external morphology not only during adult periods, but also during larval periods. In this study, we compared larval development and growth of the two pleuronectids species in detail. Fifty-eight specimens of T. kitaharae larvae and five specimens of G. stelleri larvae were collected from the East Sea, southern Korean Strait, and the adjacent waters of Jeju Island during 2014~2016, and were identified using morphological and molecular markers. Flexion larvae of T. kitaharae (10.23~16.77 mm total length, TL) and G. stelleri (14.33 mm TL) were similar in morphometrics, but can be distinguished by the melanophores in the fin membranes (present in the former vs. absent in the latter), and the melanophores at posterior margin of shoulder girdle (absent in the former vs. present in the latter). Postflexion larvae of T. kitaharae (17.12~24.92 mm TL) and G. stelleri (27.71~32.95 mm TL) differed in the number of melanophores on the mid-lateral caudal region (two in the former vs. five in the latter), and that on the dorsal and anal fin pterygiophores (five or six in the former vs. one row in the latter). Tanakius kitaharae and G. stelleri larvae differed in size at the beginning of metamorphosis. The left eye of T. kitaharae larvae between 23.13~24.92 mm TL reached the dorsal margin of the head while that of G. stelleri larvae between 38.66~42.67 mm TL did not reached yet. 가자미과 (Pleuronectidae)에 속하는 갈가자미 (Tanakius (Pleuronectidae)에 속하는 갈가자미 (Tanakius Kitaharae)와 기름가자미 (Glyptocephalus stelleri)는 성어기뿐만 아니라 초기생활기에도 외형적으로 매우 유사하여 오동정의 가능성이 높다. 따라서, 본 연구는 두 종의 자어 시기의 형태발달과정을 상세히 비교하여 종 동정의 기초자료를 제공하고자 한다. 2014~2016년 동안 동해, 남해 및 제주 근해에서 채집된 갈가자미 자어 58개체 및 기름가자미 자어 5개체를 대상으로 분자동정 및 형태 비교를 수행하였다. 갈가자미의 중기자어 (10.23~16.77 mm total length, TL)와 기름가자미의 중기자어 (14.33 mm TL)는 계측값에서 중복되나 지느러미 가장자리 흑색소포의 유무 (갈가자미는 있음 vs. 기름가자미는 없음)와 견대 뒤쪽 흑색소포열의 유무 (갈가자미는 없음 vs. 기름가자미는 있음)에서 구분되었다. 갈가자미의 후기자어 (17.12~24.92 mm TL)와 기름가자미의 후기자어(27.71~42.67 mm TL)는 계측값에서 잘 구분되었으며, 나아가 꼬리 측면 중앙부 흑색소포군의 개수 (갈가자미는 2개 vs. 기름가자미는 5개), 등지느러미와 뒷지느러미 담기골의 흑색소포 형태 (갈가자미는 5~6개의 흑색소포군이 등간격으로 분포 vs. 기름가자미는 담기골 기부를 따라 점모양 흑색소포가불규칙하게 분포)에서 차이를 보였다. 갈가자미 자어는 전장23.13~24.92 mm에서 왼쪽 눈이 머리의 등쪽 정중선에 도달하는 반면 기름가자미 자어는 전장 38.66~42.67 mm에서 왼쪽 눈이 머리의 등쪽 정중선에 도달하지 못하여 변태시기에서차이를 보였다.

Molecular and Morphometric Variations in the Sea Raven, Hemitripterus villosus from Korea, with Its Implication on Fisheries Management

장서하,이장원,김진구 한국해양과학기술원 2019 Ocean science journal Vol.54 No.3

To understand and clarify the geographic variations of the Korean sea raven, Hemitripterus villosus, we investigated the molecular and morphometric characteristics of 147 individuals (in morphs) and 175 individuals (in molecules) collected from three locations in the Yellow Sea (Incheon, Boryeong, and Heuksando), one location in the Korea Strait (Busan), and two locations in the East Sea (Goseong and Pohang), around the Korean peninsula. The Kruskal-Wallis test showed there were no significant differences in meristic characteristics among locations, except for the number of vertebrae (mean, 39.7 in Goseong vs. and 38.4−38.8 in the remaining locations). A canonical discriminant analysis based on 25 morphometric characters showed that two groups were separated by the center value “0” of CAN1: Yellow Sea and Korea Strait individuals had positive values, whereas East Sea individuals had negative values. An analysis of molecular variance (AMOVA) based on 801 basepair sequences of the mitochondrial DNA cytochrome b gene showed that two lineages were separated by fixation (FST) index. A pairwise FST test revealed moderate genetic differentiation between the Goseong individuals (middle East Sea) and the individuals at the remaining locations except for the Pohang individuals (southern East Sea). Our molecular and morphometric analysis results indicate that there exists two populations in the Korean sea raven, and their admixture zone may be located near Pohang. Population subdivision might be associated with sea level change during last glacial period, and this may have been subsequently maintained by the hydrographic and oceanic current heterogeneity of each sea around the Korean peninsula. Accordingly, different management measures should be adopted with regard to the two groups, the East Sea group and the Yellow Sea + Korea Strait group.

전라남도 칠산도 주변 참조기(Larimichthys polyactis) 어란 출현의 첫 보고

장서하,김진구,유정화 한국수산과학회 2020 한국수산과학회지 Vol.53 No.4

Chilsan-do Island, South Korea, has previously been identified as one of the biggest spawning sites of the small yellow croaker Larimichthys polyactis in the Yellow Sea. To determine whether Chilsan-do still serves as a spawning site for L. polyactis, three to five stations around Chilsan-do Island were surveyed for eggs from April to June (the main spawning season of L. polyactis) in 2019, using an RN80 net. For the first time, three L. polyactis eggs were identified at two stations, located just in front of Chilsan-do Island and between Chilsan-do and the coastline, only in May. The diameter of L. polyactis eggs (1.26-1.34 mm) was very similar to those of Collichthys niveatus (1.30-1.37 mm) and Setipinna tenuifilis (1.34-1.35 mm). During the survey period, the sea surface salinity remained constant (32.0-32.1 psu), but the sea surface temperature (SST) rapidly rose from 13.6-13.7°C in April to 22.1°C in May. Our findings suggest that L. polyactis still spawns near Chilsan-do Island today, but on a very small scale, and that changes in SST promote spawning of L. polyactis.

한국 서해남부해역 칠산도에서 채집된 민어과(농어목, 어상강) 5종 자어의 형태기재 및 분자동정

장서하 ( Seo-ha Jang ),김진구 ( Jin-koo Kim ) 한국수산해양기술학회(구 한국어업기술학회) 2021 수산해양기술연구 Vol.57 No.2

During the ichthyoplankton survey around the Chilsan Island (previously known as the biggest spawning ground of the Sciaenidae) in the southwestern sea of Korea from April to June in 2019 using ring nets, we collected a total of 12 individuals belonging to the family Sciaenidae. Using molecular methods, we identified five sciaenid species (Collichthys lucidus, Collichthys niveatus, Johnius grypotus, Nibea albiflora, and Pennahia argentata), and described and compared them on the basis of the preflexion larval stage. C. lucidus was well distinguished by the presence of occipital crests at preflexion stage. Although there were no occipital crests, preflexion larva of C. niveatus was distinguished by the absence of melanophore except for the upper part of the abdominal cavity. J. grypotus and N. albiflora were very similar morphologically, but were distinguished by myomere height (15.22-15.53% in J. grypotus vs. 11.66-12.78% in N. albiflora) in the percentage of notochord length, and eye diameter (32.58-33.37% in J. grypotus vs. 40.32-42.53% in N. albiflora) in the percentage of head length between specimens of similar size (J. grypotus: 3.22-3.23 mm, N. albiflora: 3.04-3.13 mm). P. argentata were distinguished by distribution of ventral caudal melanophore (one row of small spot in P. argentata vs. irregular patches on the central part of caudal in J. grypotus and N. albiflora). Comparative morphological studies using more diverse species must be conducted for more comprehensive understanding of the morphogenesis of Sciaenidae.

한국산 둥글넙치과 1미기록종, Kamoharaia megastoma

장서하 ( Seo-ha Jang ),김진구 ( Jin-koo Kim ),허유심 ( Yusim Heo ),유효재 ( Hyo Jae Yu ),박정호 ( Jeong-ho Park ) 한국어류학회 2018 韓國魚類學會誌 Vol.30 No.3

가자미목 둥글넙치과에 속하는 1개체 (표준체장 113.9 mm)가 제주도 남방 해역에서 저층트롤로 채집되었다. 매우 큰 입을 가지는 본 종은 주상악골의 후단이 눈보다 후방에 위치, 아래턱 전단에는 돌출된 3쌍의 날카로운 송곳니, 구강으로 돌출된 서골 말단부, 검은 가슴지느러미 그리고 무안측에 측선이 없는 특징들로 Kamoharaia megastoma와 잘 일치한다. 따라서 이종은 한국 미기록종으로 국명은 “큰입둥글넙치”로 제안한다. A single bothid specimen (113.9 mm in standard length) was collected by bottom trawl from southern Jejudo Island, Korea. It was easily identified as Kamoharaia megastoma (Kamohara, 1936), based on extremely large mouth. The species is characterized by maxillary extending beyond eyes, three pairs of long curved canines on lower jaw, tip of vomer projecting into mouth cavity, blackish pectoral fin, and blind side without lateral line. We described it as the first record to Korean fish fauna, and proposed the new Korean name, “Keun-ip-dung-geul-neop-chi” for this species.

한국 제주도에서 날개멸과(대구목) 어류, Bregmaceros nectabanus의 첫 출현 및 북서태평양 날개멸과 8종의 종검색

장서하 ( Seo-ha Jang ),김진구 ( Jin-koo Kim ) 한국수산과학회(구 한국수산학회) 2021 한국수산과학회지 Vol.54 No.3

Three specimens (33.6-44.8 mm in standard length) of the family Bregmacerotidae, collected from the waters off south-western Jeju-do Island in October 2016, were identified as Bregmaceros nectabanus on the basis of morphological and molecular methods (DNA barcoding). This species is characterized by having 50-52 2nd dorsal fin rays, 52-53 anal fin rays, 13 principal caudal fin rays, an unpigmented abdomen, irregularly distributed dorsal chromatophores, and the origin of a dorsal fin slightly behind the anal fin. This is the first reliable record of B. nectabanus in Korea. According to Yoo et al. (1992), we propose a Korean name “Tae-Pyeong-Yang-Nal-Gae-Myeol” for this species. Also, we suggest key to species of these eight bregmacerotids from the northwestern Pacific.

한국산 색가오리과(Dasyatidae) 어류 1미기록종, Hemitrygon izuensis (Nishida and Nakaya, 1988)

장서하 ( Seo-ha Jang ),김진구 ( Jin-koo Kim ) 한국수산해양기술학회 2021 수산해양기술연구 Vol.57 No.3

Two specimens of Hemitrygon izuensis (395.8-471.5 mm in disc width), belonging to the family Dasyatidae (Myliobatiformes), were collected from the waters off north-eastern Jeju-do Island in June 2016 and Pohang in August 2019. This species was characterized by having no spots on dorsal side of disc, white ventral tail fold and short preorbital snout length (15.6-18.3% of disc width). This species is similar to H. akajei and H. sinensis reported in Korea, but differs in small denticles on mid-line of dorsal surface of disc (absent in H. izuensis vs. present in H. akajei and H. sinensis), the length of ventral tail fold (28.1-31.0% in H. izuensis vs. 48.0-48.6% in H. akajei vs. 45.0% in H. sinensis), color of ventral tail fold (white in H. izuensis vs. black in H. akajei and H. sinensis) and small tubercles on the posterior part of tail (absent in H. izuensis vs. present in H. akajei and H. sinensis). We suggest a Korean name for H. izuensis as “I-ju-gal-saeg-ga-o-li” following Kim et al. (2019).