http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
조상경(Sang Kyung Jo),윤종우(Jong Woo Yoon),차대룡(Dae Ryong Cha),조원용(Won Yong Cho),김형규(Hyoung Kyu Kim),원남희(Nam Hee Won),윤수영(Soo Young Yoon),장경현(Kyung Hyun Chang) 대한내과학회 2000 대한내과학회지 Vol.59 No.6
N/A Background : Acute renal failure is a reversible process in majority of cases but mechanisms of renal injury or recovery are poorly understood. Recently neutrophil infiltration is reported to potentiate inflammatory and cytotoxic cascade in ischemic renal injury and α-melanocyte stimulating hormone has been reported to have a potent anti-inflammatory properties in a variety of animal models. We examined the beneficial effects of α-MSH in acute ischemic renal injury in rats and tried to clarify its action mechanism. Methods : After unilateral nephrectomy, renal artery of contralateral kidney was clamped for 40 minutes and reperfused in female Sprague-Dawley rats. α-MSH (50μg) and vehicle was injected intraperitoneally immediately after and 6, 24 hours after reperfusion. Biochemical, histological data, ICAM-1 mRNA, protein expressions and polymorphonuclear cell infiltration were examined. Results : α-MSH significantly attenuated the renal injury, measured by plasma BUN and creatinine level and also the degree of severity of histological injury (BUN 125.2±14.6 mg/dL : 46±19.6 mg/dL (p=0.004), creatinine 3.65±0.81 mg/dL : 1.47±0.5 mg/dL (p=0.005) at 24 hours after reperfusion, BUN 88±12.5 mg/dL : 25.5±15.8mg/dl (p=0.002), creatinine 2.76±0.5 mg/dL : 0.93±0.2 mg/dL (p=0.002) at 72 hours after reperfusion and 5.4±1.94/ 2.6±0.77 (p=0.006) at 24 hours after reperfusion in histilogical grading system). In the α-MSH treated groups, ICAM-1 mRNA expression decreased significantly compared to the vehicle treated ischemic group in 72 hours after reperfusion (0.49±0.01/0.31±0.2, p<0.008). ICAM-1 protein expression also decreased in α-MSH treated group, but it was not statistically significant. Polymorphonuclear cell infiltration showed a significant decrease in the α-MSH treated group at 24 hours after reperfusion (5.05±1.8/1.59±0.4, p=0.009). Conclusion : α-MSH attenuates ischemic renal injury by inhibiting the expression of ICAM-1 and subsequent polymorphonuclear cell infiltration. These results provide a rationale of α-MSH as a potential therapeutic drug in acute renal failure.(Korean J Med 59:641-650, 2000)
혈액투석 개수 후 단핵구배양 상청액에 의한 사람 근위 세뇨관 상피세포의 Fas 유전자 발현에 대한 연구
차대룡(Dae Ryong Cha),조원용(Won Yong Cho),윤종우(Jong Woo Yoon),조상경(Sang Kyung Jo),김형규(Hyung Kyu Kim),장경현(Kyung Hyun Chang) 대한신장학회 2000 Kidney Research and Clinical Practice Vol.19 No.3
N/A Residual renal function rapidly declines after the initiation of hemodialysis and its mechanisms are supposed to be associated with frequent hypotensive episodes during hemodialysis and subsequent ischemic injury to remnant nephron, but blood-membrane interaction might play an important role because of its ability to activate complement system and other various humoral and cellular mechanisms. Blood monocytes are activated by complements, bacterial conta- minants and activated monocytes are known to secrete multiple proinflammatory cytokines such as TNF-α, IL-1β. The expression of TNF-αand IL-1β in mRNA and protein level were examined by RT-PCR and ELISA respectively in patients with ESRD after the initiation of hemodialysis. Author also investigated the mRNA expression of Fas in human proximal tubular cell culture in the presence of TNF-a and PBMC culture supernatant before and after the ini-tiation of hemodialysis. Compared to PBMC separated before the initiation of HD, the amount of cytokine mRNA from PBMC separated after the initiation of HD showed increased tendency from 0.97±0.2 to 1.12±0.28 for TNF-α(p=0.29), from 1.03±0.18 to 1.10±027 for IL-1β (p=0.54). TNF-αand IL-lβ protein level in PBMC culture supernatant also showed increased tendency from 2.25±0.5 to 4.254±3.77 for TNF- α(p=0.10), from 3.5±2.08 to 4.0±4.3 for IL-1β(p=0,25). TNF-a in-creased Fas mRNA expression dose-dependently com-pared to control but it was not stat.istically significant(p=0,37, 0.22). Compared to the the level of Fas expression in HPTC cultured in the presence of pre HD PBMC supernatant, the level of Fas expression increased significantly in the presence of post HD PBMC supernatant(0.64±057 vs 1.05±0.12, p=0.01). As a conclusion, cytokine gene expression and secretion can increase as a result of blood-membrane interaction and these might have some influence on the loss of residual renal function in CRF patients maintained on hemodialysis.