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메틸오렌지에 의한 나일론의 염색에 있어서 열역학적 특성 -소수결합의 영향-
임용진,윤남식,이창모,Im, Yong-Jin,Yun, Nam-Sik,Lee, Chang-Mo 한국섬유공학회 1987 한국섬유공학회지 Vol.24 No.4
The bindings of nylons with methyl orange were studied, and the first binding constants and thermodynamic parameters for the binding were obtained. It was found that, with increasing the methylene group of the nylons, the free energy for the binding increased negatively and the enthalpy and the entropy increased positively. The favorable free energy change for the binding appeared to be a result of favorable entropy change rather than enthalpy change. All these facts obtained can be interpreted by the hydrophobic interaction between the hydrocarbon moiety of the dye and the hydrocarbon parts of the nylons, and it is reasonable to conclude that not only the electrostatic attraction but also the hydrophobic interaction contributes to the binding of methyl orange, a typical acid dye, by nylons.
임용진,윤남식,장진규,Im, Yong-Jin,Yun, Nam-Sik,Jang, Jin-Gyu 한국섬유공학회 1987 한국섬유공학회지 Vol.24 No.5
To investigate the effect of carrier on the dyeability of poly(ethylen terephthalate)(PET) fiber, equilibrium dye uptake and initial dyeing temperature were measured and discussed in connection with solubility parameter. It was shown that the equilibrium dye uptake for carrier-added dyeing increased with smaller component solubility parameter difference between PET and carrier, and lower initial dyeing temperature. For carriers with similar solubility parameter and molar volume, the one which has phenyl ring and carbonyl group in its molecular structure exhibited greater carrier activity. In this experiment, the most effective carrier for PET fiber was benzaldehyde and acetophenone, which can interact with PET fiber most effectively by overlap of p-orbitals.
메틸오렌지계 염료에 의한 나일론의 염색에 있어서 수결합의 영향
임용진,윤남식,송성한,Im, Yong-Jin,Yun, Nam-Sik,Song, Seong-Han 한국섬유공학회 1989 한국섬유공학회지 Vol.26 No.1
The contribution of hydrophobic interaction to the binding of nylons with methyl orange and its homologs was investigated from the thermodynamic analysis of the binding system. It was found that the free energy increased negatively with the hydrophobicity of dye and nylon against unfavorable enthalpy situation in the binding system, which means that the binding is entropy-driven. The contribution of hydrophobic interaction to the binding was much pronounced in the binding of nylon 612 with butyl orange where the free energy increases negatively in spite of endothermic enthalpy situation. These results show that hydrophobic interaction contributes significantly to the binding of nylon with acid dye, and that not only hydrophobicity of nylon but also that of dye affects the hydrophobic interaction.
김우식,이석기,임용진,Kim, U-Sik,Lee, Seok-Gi,Im, Yong-Jin The Korean Fiber Society 1994 한국섬유공학회지 Vol.31 No.9
The crossllnked poly(4-vinylpyridine) was examined for its ability to bind methyl orange at various temperatures in ethylene glycol. The first binding constants(K1) were evaluated. These values were compared with those of aqueous solution. It was found that the values of Kl in ethylene glycol were smaller than those in aqueous solution and the values showed a bell-shaped curve against the binding temperature. These results are discussed in terms of the swelling degree and the crosslinked hole size.
HPLC를 이용한 기능성 원료 지표성분으로서 장수 오미자 분말 중 Schizandrin의 분석법 개발
김윤정(Yunjeong Kim),하나(Na Ha),한송희(Song-Hee Han),전지영(Ji-Young Jeon),황민호(Minho Hwang),임용진(Yong-Jin Im),이선영(Sun Young Lee),채수완(Soo-Wan Chae),김민걸(Min-Gul Kim) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.2
본 연구는 HPLC를 이용하여 건강기능식품 기능성 원료로서 오미자의 지표성분인 schizandrin의 분석법을 확립하고 이에 대한 분석법 검증을 실시하고자 하였다. 그 결과 본 시험법에서 표준용액의 피크유지시간과 오미자추출액의 피크유지시간이 일치하여 특이성을 확인하였다. 검량선의 상관계수(R²)는 0.9990 이상으로 높은 유의수준을 보여 분석에 적합함을 알 수 있었으며, 검출한계는 0.2 μg/mL, 정량한계는 0.5 μg/mL로 설정되었다. 일내, 일간분석에서 정밀도를 나타내는 변동계수(CV, coefficient variation)는 표준용액에서 각각 0.06~0.66%, 0.13~1.19%로 나타내었고 정확성(accuracy)은 각각 98.35~103.30%, 98.35~103.00%로 나타내어 오미자의 지표성분인 schizandrin의 분석법이 적합한 시험법임이 검증되었다. 본 분석법에 따라 장수오미자분말에서 schizandrin을 분석한 결과 대한약전의 분석법보다 빠르며 다른 물질의 간섭 없이 분석 가능하여 건강기능식품의 기능성원료 표준화에 응용할 수 있을 것으로 판단된다. We studied the development of schizandrin as a marker compound in Jangsu Omija. Schizandrin was validated for its LOD (limit of detection), LOQ (limit of quantitation), precision, accuracy, and recovery by HPLC relative to Omija powder. It showed a high linearity in the calibration curve with a coefficient of correlation (R²) of 0.9990. The LOD and LOQ were 0.2 μg/mL and 0.5 μg/mL, respectively. The intra- and inter-day precision of schizandrin calibration standards was 0.06~0.66% and 0.13~1.19%, respectively, and the intra- and inter-day accuracy of schizandrin was 98.35~103.30% and 98.35~103.00%, respectively. Overall, schizandrin was validated through our analytical methods as a marker compound in Jangsu Omija.
LC-MS/MS를 이용한 인체 혈장에서 Ginsenoside Rb1의 분석법 검증
한송희(Song-Hee Han),김윤정(Yunjeong Kim),전지영(Ji-Young Jeon),황민호(Minho Hwang),임용진(Yong-Jin Im),이선영(Sun Young Lee),채수완(Soo-Wan Chae),김민걸(Min-Gul Kim) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.12
LC-MS/MS를 사용함으로써 인체 혈장에서 ginsenoside Rb1의 분석법을 개발하고 검증하였다. 유속 0.9 mL/min에 이동상 0.1% formic acid가 첨가된 water와 methanol을 사용하여 기울기 용리 조건으로 설정하였으며 사용한 분석 칼럼은 C 18(4.6 mm×150 mm, particle size 5 μm)을 사용하여 분리하였다. MRM(multiple reaction monitoring) 방법의 전기 분무 이온화 이온 분석기로 모니터링 하여 분석하였다. 인체 혈장 샘플은 액체-액체 추출방법에 의해 acetone과 water가 섞인 용액으로 추출하였다. 이 분석의 검량선 범위는 10~500 ng/mL이며 상관계수는 0.9995를 나타냈다. 일내, 일간의 정밀성 농도범위는 상관계수 5.8% 그리고 정확성은 96.0~104.6%로 나타났다. 이 LC-MS/MS를 이용한 인체 혈장의 ginsenoside Rb1의 연구가 약동학 연구에 적용할 수 있을 거라 생각한다. A new liquid chromatographic tandem mass spectrometric (LC-MS/MS) assay for the quantification of ginsenoside Rb1 in human plasma was developed and validated. The separation was performed on a Agilent C18 column (4.6 mm×150 mm, particle size 5 μm) with a gradient elution of 0.1% formic acid in water and 0.1% formic acid in methanol and a flow rate of 0.9 mL/min. The analyte was determined using electrospray positive ionization mass spectrometry in the multiple reaction monitoring (MRM) mode (m/z 1131.714→365.303). Human plasma samples were extracted with acetone : water (50:50) by the liquid-liquid extraction method. The method was linear over the dynamic range of 10~500 ng/mL with a correlation coefficient of r=0.9995. The intra-and inter-day precision over the concentration range of ginsenoside Rb1 was lower than 5.8% (correlation of variance, CV), and the accuracy was between 96.0~104.6%. This LC-MS/MS assay of ginsenoside Rb1 in human plasma is applicable for quantification in a pharmacokinetic study.
고창 복분자의 기능성원료 표준화를 위한 지표성분으로서 Ellagic Acid의 분석법 개발
김윤정(Yunjeong Kim),한송희(Song-Hee Han),전지영(Ji-Yeong Jeon),황민호(Minho Hwang),임용진(Yong-Jin Im),채수완(Soo-Wan Chae),김민걸(Min-Gul Kim) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.11
고창 복분자의 개별인정형 건강기능식품 기능성 원료로 개발하기 위하여 지표성분 표준화를 위한 ellagic acid의 분석법 설정과 분석법에 대한 밸리데이션을 실시하였다. 1% formic acid가 첨가된 water와 acetonitrile을 이동상으로 하고 Symmetry®(C18, 4.6×250 ㎜, 5.0 ㎛) 칼럼을 사용하여 기울기용리(gradient elution) 방법으로 분석하였다. 본 연구에서는 분석법을 확립하고 분석법에 대하여 특이성, 직선성, 정확성과 정밀성 그리고 회수율에 대하여 확인하였다. Ellagic acid의 검량선은 R2=0.9996으로 좋은 선형성을 보였으며 LLOQ와 LOD는 각각 1 ㎍/mL와 0.3 ㎍/mL였다. 일내와 일간 분석에서 상대표준편차(RSD)는 각각 2.28%와 2.84% 미만으로 나왔다. 회수율 측정결과에서는 89.17~97.92%로 나왔고, RSD는 0.05~0.14%였다. 그러므로 HPLC를 이용한 ellagic acid의 분석법이 고창 복분자 추출물의 기능성원료 표준화를 위한 지표성분 분석을 위한 적합한 시험법임이 검증되었다. 본 시험법에 따라 분석한 고창 미숙과 복분자 추출물 내의 ellagic acid의 함량은 세 lot를 3번씩 분석하였을 때 약 1.92 ㎍/㎎(0.192%)이 나왔으며 RSD값은 2.36% 이하였다. 따라서 본 연구를 통하여 확립된 ellagic acid의 분석법이 고창 복분자의 개별인정형 건강기능식품 기능성 원료개발을 위한 기초자료로 활용될 것으로 본다. Method development and validation of ellagic acid for the standardization of Gochang Bokbunja as a functional ingredient and health food were accomplished. A Symmetry® (C18, 4.6×250 ㎜, 5.0 ㎛) column was used with a gradient elution system of 1% formic acid in water and acetonitrile. This method was validated according to specificity, linearity, accuracy, precision test, and recovery test. Specificity was confirmed with identical retention time, and calibration curves of ellagic acid showed good linear regression (R2>0.9996). Relative standard deviations (RSD) of data from the intra- and inter-day experiments were less than 2.28% and 2.84%, except in the low limit of quality control (LLOQ, 1 ㎍/mL) sample. The results of the recovery test were from 89.17% to 97.92% with RSD values from 0.05 to 0.14%. Therefore, we performed analysis of ellagic acid as a marker compound in Gochang Bokbunja extracts. The amount of ellagic acid in Gochang Bukbunja was about 1.918 ㎍/㎎ (0.192%) in the three times analysis, and RSD was less than 2.36% by the validated method. These results suggest that the developed HPLC method is simple, efficient, and could contribute to the quality control of Gochang Bokbunja extract as a functional ingredient.