한국 남성 불임환자에서 Y 염색체상의 AZF Gene에 대한 분석 및 DAZ Gene의 발현 양상

이호준,이형송,송견지,변혜경,서주태,김종현,이유식,Lee, Ho-Joon,Lee, Hyoung-Song,Song, Gyun-Jee,Byun, Hye-Kyung,Seo, Ju-Tae,Kim, Jong-Hyun,Lee, You-Sik 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.1

Cytogenetic observations of loss of the distal portion of the Y chromosome long arm were found to be associated with disrupted spermatogenesis. The existence of a gene involved in the regulation of spermatogenesis, the azoospermia factor (AZF), was postulated. In this study, we screened the AZF region including DAZ and DAZH genes and observed the expression pattern of DAZ and DAZH transcript in infertile men with azoospermia and oligospermia by using a sequence-tagged site (STS)-based PCR method. PCR primers were synthesized for 11 STSs that span Yq interval 6, SRY, DAZ, and DAZH, functional DAZ homologue on chromosome 3. Microdeletions were detected in 4/32 (12.5%) azoospermic men and 1/11 (9%) severe oligospermic men. Only 2 of 5 patients had microdeletions of Yq that contained the DAZ gene, whereas the other 3 patients had deletions extending from intervals 5L-6F proximal to the DAZ gene on Yq. Testis biopsies of the azoospermic patients revealed a variety from Sertoli cell-only syndrome to testicular maturation arrest. Of 4 men with clinical data available, average testis size was R: 13.8 cc, L: 13.8 cc, serum T was $4.0{\pm}1.25$ ng/ml, LH was $3.63{\pm}1.90$ mIU/ml, and FSH was $8.85{\pm}5.13$ mIU/ml. These values did not differ significantly from the remainder of the patients tested. We could not observed the DAZ transcript in 2 patients, who have no mature spermatozoa. In 11.6% of patients microdeletions of the AZF could be detected. These deletions in the AZF region seem to be involved causing spermatogenic failure. But the frequency of microdeletions proximal to DAZ suggests that DAZ is not the only gene associated with spermatogenic failure.

Miscanthus sinensis 와 타 식물과의 친근성에 관한 연구

정일구(Il Koo Lee),이호준(Ho Joon Lee),이경순(Kyung Soon Lee) 한국식물학회 1970 Journal of Plant Biology Vol.13 No.4

Althought allelopathy has been cleary established for Pinus densiflora by Lee-Monsi, the auther suspected that Miscanthus sinensis which grows vigrously in such Korean pine forests might also exhibit the phenomenon. A total of 33 species having an affinity in the Miscanthus group were recorded in the field. Out of these, five species; Patrinia scabiosaefolia, Lespedeza crytobotrya. Oenothera odorata, Raphanus sativus var. acanthiformis, and Zoysia japonica were considered the A group. Five other species which have no affinity to Miscanthus sinensis, Amaranthus patulus, Solanum nigrum, Capsella bursa-pastorris var. triangularis, Chemopodium album var. centrorubrum and Alopeculus amurensis were considered the B group. Extracts of Miscanthus sinensis were applied to determine relative germination and growth. It was found the growth in the B group was promoted by a cold water extract of Miscanthus stems and leaves, but inhibited remarkably by an extract obtained by passing cold water through Miscanthus root. As expected, growth in the A group was promoted by both experimental treatments.

월악산 신갈나무 ( Quercus mongolica ) 림의 종조성과 토양환경

이호준,전영문,김창호 ( Ho Joon Lee,Young Moon Chun,Chang Ho Kim ) 한국환경생물학회 1998 환경생물 : 환경생물학회지 Vol.16 No.2

The relationship between the floristic composition and soil environmental factors of the Quercus mongolica forest on Mt. Worak was investigated from May 1993 to February 1997. The Quercus mongolica -Lespedeza maximoiwiczii community was classified into two subcommunity of Quercus variabilis and Artemisia stolonifera. The dominance sequences on each stratum determined by the coverage index showed that Quercus mongolica was most dominant in tree-1 layer with 7569.6, and in tree-2 layer with 1211.4, Lespedeza maximoiwiczii in shrub layer with 896.9, and Ainsliaea acerifolia in Herb layer with 1480.8 value. The highest value of diameter breast height (DBH) was distributed in the range of 11 ∼ 20 ㎝ (41.1%) in Quercus mongolica forest and it showed a bell-shape distribution. Except soil water content, almost every data showed the lower value at 600∼850m than any other altitude. Species dominance was eminently Sasu borealis. Carex lanceolata at 400∼600m, Carex humilis at 600∼850 m and Fraxinus rhynchophylla, Acer pseitdo-sieboldianum, Ainsliaea acerifolia, Carex siderosticta at 860∼1060m, respectively. Soil conditions in NE-NW slope was more favorable than in SE-SW slope. In summary, Quercus mongolica, Acer pseudo-sieboldianum. Carex siderosticta, and Ainsliaea acerifolia were dominant in NE-NW slope, while Quercus mongolica, Fraxinus rhynchophylla, Spodiopogon sibiricus, and Carex humilis dominant in SE-SW slope.

일본 식품 용기포장재의 안전성에 대한 규격과 최신동향

이호준,Lee, Ho-Joon 한국식품연구원 2009 食品技術 Vol.22 No.4

광섬유의 음향센서 기법

이호준,박병철,Lee, Ho-Joon,Park, Byung-Chul 한국음향학회 1990 韓國音響學會誌 Vol.9 No.2

다엽제한기 소조사면의 6 MV 광자선 출력선량계수

이호준,최태진,오영기,전경수,이용희,김진희,김옥배,오세안,김성규,예지원,Lee, Ho Joon,Choi, Tae-Jin,Oh, Young Kee,Jeun, Kyung Soo,Lee, Yong Hee,Kim, Jin Hee,Kim, Ok Bae,Oh, Se An,Kim, Sung Kyu,Ye, Ji Woon 한국의학물리학회 2014 의학물리 Vol.25 No.1

종양부위의 입체적이고 선택적인 치료가 가능해 임상표적부피(clinical target vlume, CTV)에 높은 선량으로 집중조사하고 부작용을 현저히 줄이는 세기조절방사선치료는 치료예후를 향상시키고 있다. 방사선세기조절 치료는 MLC의 개방면적과 개방시간으로 조사면내 플루언스를 조정하므로 소형조사면의 선량이 누적되어 원하는 선량이 조사하게 된다. 따라서 소형조사면과 계층형 조사면의 출력선량계수의 정확성은 곧 Portal MU 결정에 정확성을 더할 수 있고, 종양에 조사되는 선량의 정확성을 향상할 수 있으므로, 이 연구는 Clinac Ex (Varian) $3{\times}3cm^2$에서 $0.5{\times}0.5cm^2$까지 조사면을 선정하였고 방사선은 6 MVX선의 소형조사면의 출력선량계수를 평가하였다. 조사면은 다엽제한기를 $40{\times}40cm^2$로 개방하고 Collimator jaw를 이용한 것과 Collimator를 $10{\times}10cm^2$로 고정하고 다엽제한기에 의한 조사면으로 구분하여 출력선량계수가 결정되었다. 검출기는 유효체적이 $0.01cm^3$이고 내경 2 mm인 CC01 (Scanditronix-Wellope)이온전리함과 SFD 다이오드 검출기(0.6 mmØ, $500{\mu}m$ 두께, Scanditronix-Wellope)와 다이아몬드 검출기(T60003, PTW)와 X-Omat film을 사용하였다. 결과는 다엽제한기 조사면의 출력선량계수는 $3{\times}3cm^2$에서 $0.899{\pm}0.0106$, $2{\times}2cm^2$에서 $0.855{\pm}0.0106$, $1{\times}1cm^2$에서 $0.764{\pm}0.0082$, $0.5{\times}0.5cm^2$에서 $0.602{\pm}0.0399$를 얻었다. Jaw를 $10{\times}10cm^2$로 고정하고 다엽제한기의 조사면의 출력계수는 MLC를 $40{\times}40cm^2$에 jaw에 의한 소형조사면의 것보다 최대 3.8% 높게 나타남을 확인하였다. 따라서 세기조절방사선치료 TPS에는 collimator jaw 보다 다엽제한기 조사면 크기의 출력선량계수가 설정되는 것이 중요함을 의미한다. The IMRT is proper implement to get high dose deliver to tumor as its shape and selective approach in radiation therapy. Since the IMRT is performed as modulated the radiation fluence by the MLC created the open shapes and its irradiation time, the dose of segment of radiation field effects on the cumulated portal dose. The accurate output factor of small and step shape of segment is important to improve the determination of deliver tumor dose as it is directly proportional to dose. This experiment performed with the 6 MV photon beam of Clinac Ex(Varian) from $3{\times}3cm^2$ to $0.5{\times}0.5cm^2$ small field size for collimator jaw in MLC free and/or for MLC open field in fixed collimator jaw $10{\times}10cm^2$ using the CC01 ion chamber, SFD diode, diamond detector and X-Omat film dosimetry. As results of normalized to the reference field of $10{\times}10cm^2$ of MLC, the output factor of $3{\times}3cm^2$ showed $0.899{\pm}0.0106$, $0.855{\pm}0.0106$ for $2{\times}2cm^2$, $0.764{\pm}0.0082$ for $1{\times}1cm^2$ and $0.602{\pm}0.0399$ for $0.5{\times}0.5cm^2$. The output factor of MLC open field has shown a maximum 3.8% higher than that of the collimator jaw open field.

고해상도 레이더용 다중산란점 발생장치의 설계 및 제작

이호준,김윤진,윤승구,정해창,공덕규,이재웅,변영진,Lee, Ho-Joon,Kim, Youn-Jin,Yoon, Seung-Gu,Jeong, Hae-Chang,Kong, Deok-Kyu,Yi, Jae-Woong,Byun, Young-Jin 한국군사과학기술학회 2016 한국군사과학기술학회지 Vol.19 No.5

We designed the multiple scattering points generation system to simulate an actual situation of target signal for high range resolution radar system. This provides replicating the target signals and controlling the status of target signals for radar system. This is composed transmit antenna and multi target generator. Transmit antenna is waveguide array antenna and multi target generator has signal distribution module and control & power module. Multi target generator is able to provide the high isolation and variable output power. Moreover, in order to monitor all output signals of the multi target generator, the flows of signals are programed in control & power module. The performance is demonstrated using experimented results of high range resolution radar.

Mn 과 Zn 이 소리쟁이 ( Rumex crispus L. ) 의 발아와 유식물의 (幼植物) 생장에 미치는 영향

이호준,윤영진 ( Ho Joon Lee,Young Jin Yoon ) 한국환경생물학회 1994 환경생물 : 환경생물학회지 Vol.12 No.2

The amounts of accumulation and the effects of manganese and zinc on the seed germination and the growth of seedlings were investigated in Rumex crispus L. after Mn and Zn treatments. The effects of Mn and Zn on the germination rate in R. crispus L. showed 84∼89% in the treatments of the below 50ppm in both Mn and Zn, whereas it showed 65% in 100ppm Mn and 79% in 100ppm Zn, which had a relatively high germination rate in high concentration. The relative growth rate in R. crispus L. exhibited a tendency of somewhat restrained growth in the higher concentration, but showed a good growth compared to control. The relative growth rate of length in R. crispus L. as concentrations of Mn and Zn increase from 5ppm to 100ppm revealed a tendency of somewhat restrained growth, but showed a good growth compared to control. The accumulation amount of Mn and Zn in organs of R. crispus L. showed a tendency to increase in order of its stem < leaf < root, and the more time passed by after its treatment and the higher the concentrations of its treatment were, the more amount of their accumulation appeared. These results suggest that R. crispus L. is not only strong in tolerance in the contaminated soil of Mn and Zn but contributable to the purification of the contaminated soil.

동결보존에 의한 인간배아의 생존률과 임신에 관한 연구

이호준,이승재,노성일,백혜란,김문규,Lee, Ho-Joon,Lee, Seung-Jae,Roh, Sung-Il,Paik, Hye-Ran,Kim, Moon-Kyoo 대한생식의학회 1990 Clinical and Experimental Reproductive Medicine Vol.17 No.2

This study was done to verify factors affecting viability after cryopreservation and pregnancy rate after frozen-thawed embryo transfer into uterus. Embryos were cryopreserved slow freezing and slow thawing and used DMSO as cryoprotectant. The results were to follows. 1. Viability of frozen-thawed embryos were 75.5% (94/105), which compared with viability of embryos according to cell stage, $2{\sim}5$ cell was 68.4% and $6{\sim}16$ cell 80.4% were significant differences (p<0.05). 2. No significant difference in duration of cryopreservation on effects affecting pregnancy rate was observed. 3. Number of embryo transfered into uterus was significant differences (p<0.05). 4. Four pregnancies resulted following replacement of 35 frozen-thawed.

프로피온산을 이용한 시리얼제품의 방충 포장

이호준,Lee, Ho-Joon 한국식품연구원 2011 食品技術 Vol.24 No.2