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항진균성 항생물질을 생산하는 Bacillus sp. LAM 97-44의 분리 및 동정
이노운,김천석,도재호,정인찬,이현우,이동희,Lee, No-Woon,Kim, Cheon-Suk,Do, Jae-Ho,Jung, In-Chan,Lee, Hyean-Woo,Yi, Dong-Heui 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.33 No.2
항진균제를 개발하기 위하여 토양으로부터 700주의 세균, 방선균, 곰팡이를 분리하였으며, 이들 배양액을 조사한 결과 Candida albicans의 성장을 억제시킬 수 있는 LAM 97-44 균주를 항진균성 항생물질 생산균으로 선발하였다. 분리균주를 동정하기 위해 생산균 LAM 97-44 균주의 형태학적 특성, 배양학적 특성, 생리학적 특성, 균체성분 등을 조사하였다. LAM 97-44 균주는 $2{\sim}3{\times}1{\sim}1.5\;{\mu}m$ 크기의 포자를 형성하는 간균이며, 포자의 형태는 ellipsoid 형 이었다. LAM 97-44 균주는 arabinose, cellulose, xylose를 이용하지 못하였으나 fructose, glucose, glycerol, maltose, raffinose 등은 이용하는 것으로 나타났다. 지방산 분석결과는 iso-와 anteiso-인 성분으로 구성되어 있었으며, menaquinone은 Bacillus속 세균의 전형적인 isoprenoid 사슬이 7개인 menaquinone(MK-7)이었다. 이러한 결과를 종합하면 LAM 97-44 균주는 Bacillus subtilis와 유사한 균으로 동정되었다. In order to develop an effective antifungal antibiotics, over 700 isolates of bacteria, mold and actinomytes were screened from soil, and LAM 97-44 were selected as a strain producing the strong antifungal antibiotics against Candida albicans. Morphological, cultural and physiological characteristics of LAM 97-44 were investigated for the indentification. The cell size of LAM 97-44 was $2{\sim}3{\times}1{\sim}1.5\;{\mu}m$, and the shape of spore was of ellipsoidal. As a carbon source, LAM 97-44 utilized fructose, glucose, glycerol, maltose and raffinose but did not utilize arabinose, cellulose and xylose. The fatty acids of the cells included various iso-type and anteiso-type. Conclusively, the strain LAM 97-44 was proved to be Bacillus subtilis.
Streptomyces aureofaciens 동결건조후 재수화 방법이 생존도에 미치는 영향
이노운(No Woon Lee),이현우(Hyean Woo Lee),이동희(Dong Heui Yi) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.6
In order to examine the effect of rehydration methods on viability after freeze-drying of Streptomyces aureofaciens, we labeled the DNA of S. aureofaciens with ³H-adenine. Extracellular radioactivity levels appeared to be high in the rehydrated solutions after freeze-drying than freezing-thawing. In effects of rehydration after freeze-drying, the viability of the cell appeared about 20% in case of with admitting air at single ampule, but that of which appeared about 91% in case of without admitting air at double ampule. Thus, S. aureofaciens cells were damaged during the process of rehydration after freeze-drying.
항진균성 항생물질를 생산하는 Bacillus sp. LAM 97-44 의 분리 및 동정
이노운,김천석,도재호,정인찬,이현우,이동희 ( No Woon Lee,Cheon Suk Kim,Jae Ho Do,In Chan Jung,Hyean Woo Lee,Dong Heui Yi ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.3
In order to develop an effective antifungal antibioltics over 700 isolates of bacteria, mold and actinomytes were screened from soil., and LAM 97-44 were selected as a strain producing the strong antifungal antibiotics against Candida albicans. Morphological, cultural and physiological characteristics fo LAM 97-44 were investigated for the indentification. The cell size of LAM 97-44 was 2∼3×1∼1.5 ㎛, and the shape of spore was of ellipsoidal. As a carbon source, LAM 97-44 utilized fructose, glucose, glycerol, maltose and raffinose but did not utilize arabinose, cellulose and xylose. The fatty acids of the cells included various iso-type and antciso-type. Conclusively, the strain LAM 97-44 was proved to be Bacillus subtilis.
구입경로가 다른 두 종류의 육미지황탕을 투여한 흰쥐의 혈액분석연구
전성진,이선동,박해모,최종환,이현우,Jeon, Seong-Jin,Lee, Sun-Dong,Park, Hae-Mo,Choi, Jong-Hhan,Lee, Hyean-Woo 대한예방한의학회 2005 대한예방한의학회지 Vol.9 No.2
Traditional herbal medicine is widely used among the Korean people, and other eastern Asian countries employ similar therapies as well. In recent years, due to increasing interest in herbal medicine, many researches have been made on the toxicity and adverse drug reactions of herbal medications. Through private and public media, there have been many opinions that taking herbal medicine is very harmful, especially, to liver and kidney. We face upon evaluation of herbal medication, safe, and efficacy. Furthermore, we need to control quality of herbs. This study aims to verify the evidence that taking herbal medicine will yield equal reaction in 2 lab animal groups (A and B). One frequently prescribed herbal medication, Yugmijihwangtang, was used to test the evaluation of quality on lab animals (SD-Rat). There were no significant differences in body, visceral weight, and serum analysis test results after herbal medication for 1 month. But, AST and ALT scores were raised in 2 subjects in group A (over reference range). It seems to be an adverse drug reaction, and this finding was restricted in group A herbal medicine. These results suggest that we need to qualify herbal plants in Korea, and study which herbs would cause specific reactions in human.
세포응집물질을 생산하는 Aspergillus sp. LAM 94-142의 특성 및 물질생산 조건
이동희,이노운,이현우 건국대학교 산업기술연구원 1996 건국기술연구논문지 Vol.21 No.-
토양에서 분리한 세포응집물질을 생산하는 균주 LAM 94-142는 형태, 배양 및 생리학적 특성을 조사한 결과 Aspergillus parasiticus 또는 그 근연균으로 동정되었으며 물질을 생산하기 위한 배양최적 조건을 검토한 결과는 다음과 같다. 최적 탄소원과 질소원은 각각 1.5% glucose와 0.35% yeast extract였으며 Ca²+을 첨가하였을 때 물질생산이 현저히 증가되었으며 K+, Cu²+, Fe²+ 등의 금속이온은 물질 생성을 저해하였다. 또 인산의 K염은 물질 생합성을 촉진하였으나 ammonium염은 크게 저해하였다. 그리고 최적 초발 pH와 온도는 pH7과 30℃였으며 4일간 배양하였을 때 물질 생산량이 최대였다. A strain of mold, LAM 94-142, capable of producing cell aggregation factor was isolated from soil and identified as Aspergillus parasiticus or its related strain on the morphological, cultural and physiological characteristics. For production of the cell aggregation factor, glucose and yeast extract was favorable as carbon and nitrogen source, respectively. The factor production was increased by the addition of Ca²+ and potassium phosphates but inhibited by K+, Cu²+, Fe²+ and ammonium phosphates. Optimal initial pH and temperature for the factor production was pH 7 and 30℃, respectively. The production of the factor reached at maximum after 4 days in the medium containing 1.5% glucose, 0.35% yeast extract, 0.07% CaCl₂, and 0.05% KH₂PO₄.
Cordyceps scarabaeicola KEFC-C252의 균사체 배양에 의한 수용성 색소의 생산과 색소의 항돌연변이 효과
이현우,손준형,최종환,예병일,신운섭,김중배,김현원 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.2
Cordyceps scarabaeicola KEFC-C252 균사체의 배양으로 생산되는 천연색소의 생산조건 및 색소의 정제방법을 조사하였으며, 분리된 색소의 항돌연변이 효과를 조사하였다. 색소의 생산에 적합한 배지는 1.5% sucrose, 2.5% yeast extract, 초기 pH5.5였다. C. scarabaeicola KEFC-C252를 26℃에서 108시간 배양한 후에 색소생산이 최대에 달하였으며, 최적조건에서 C. scarabaeicola KEFC-C252는 1.2 g/liter의 색소를 생산하였다. 색소는 ethylacetate 추출, 산침전 및 결정화과정을 거치면서 정제하여 황색의 육각기둥 모양의 결정을 얻었다. 분리된 색소는 용액의 pH변화에 따라 색상이 변하는 특성을 나타내었는데 알칼리성 용액에서 청색, 산성 용액에서 붉은색을 나타내었다. 분리된 색소는 4-NQO로 유도된 돌연변이를 억제하는 항돌연변이원성 물질이었다. 또 분리된 색소는 Salmonella typhimurium TA98과 TA100에서 자발적 돌연변이를 감소시켰다. Cultural conditions for the production of water-soluble pigment from mycelial culture of Cordyceps scarabaeicola KEFC-C252 and antimutagenic activity of the pigment were investigated. To obtain the maximum productivity of the pigment from mycelial culture of C. scarabaeicola KEFC-C252, the optimized medium was made with 1.5% sucrose, 2.5% yeast extract and initial pH 5.5. C. scarabaeicola KEFC-C252 was cultivated to reach the maximum concentration of the pigment at 26℃ for 108 hrs. C. scarabaeicola KEFC-C252 produced about 1.2 g/liter pigment under the optimized condition. The pigment was isolated from the culture filtrate by ethylacetate extraction, acidic precipitation and crystallization. The isolated pigment was scarlet hexagonal column crystal, and the color of the pigment was changed according to pH of the solution. The pigment showed violet in the alkaline water but showed red color in the acidic water. The pigment showed inhibitory activity against mutagenic activity induced by 4-nitroquinoline N-oxide. Furthermore, the pigment showed inhibitory activity against spontaneous mutation on Salmonella typhimurium TA98 and TA100.