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Flurescent In Situ Hybridization방법으로 분석한 소양호 세균 군집 구조의 졔절적 변화
홍선희,안태석 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-
소양호에서 세균군집의 계절적, 수심별 변화를 파악하고자 총세균수와 EUB338, ALF1b, BET42a, GAM42a와 CF probe 등 fluorescent rRNA targeted oHgonucleotide probe와 반응하는 세균 개체수를 측정하였다. 총세균수는 0.5~2.01×10^(4)cell·㎖이였으며, 2m와 5m 수층에서 높게 나타났다. 총세균수에 대한 Eubacteria의 비율은 22~100%였고 Proteobacteria α-group은 Eubacteria의 2.6~66.7%, β-group은 4.5~53.5%, γ-group은 4.6~7.6%, 그리고 Cylophaga-Flavobacterium group은 2.1~35.9%이였다. 또한 세균군집은 계절별, 수심별로 다양한 변화를 보여, 겨울철을 β-group이, 봄철과 초여름철은 γ-group이, 여름철은 α-group이 우점하였고, Cytophaga-Flavobacterium group이 특징적으로 우점하는 시기는 없었다. 이러한 세균 군집 구조의 분포로 계절별, 수심별로 호수에 대한 독특한 특징을 알 수 있었다. To define the structure and diversity of bacterial communities in the aqutic ecosystem, Lake Soyang, the largest artificial reservoir in Korea, a new method, fluorescent in situ hybridization was applied. This technique relies on the specific hybridization of the nucleic acid probes to the naturally amplified intracellular rRNA. By this method, the bacterial community composition of Lake Soyang and bacterial numbers belong to eubacteria, proteobacteria and Cytophaga-Flavobacteria group were estimated. Total bacterial numbers ranged from 0.3×10^(6)~2.O×10^(6)cells · ml^(-1), and vertical profile of total bacteria showed the peak at 2 and 5 m depths. The ratio of eu-bacteria to total bacteria were 22~100% and varied wuth depth and season. The percentage of Prothobacteria α-group ranged 2.6~66.7%, β-group 4.5~ 53.5%, γ-group 4.6~70.7% and Cytophaga-Flavobacterium group 2.1~35.9%. Also, bacterial community had spatial and temporal characteristics. The dominant groups were β-group in winter, γ-group in spring and early summer and α-group in summer.
봄철 Cyanobacteria 가 우점한 천호지에서 세균군집구조의 변화
홍선희,전선옥,안태석,안태영 한국미생물학회 2002 미생물학회지 Vol.38 No.4
봄철 cyanobarteria가 우점하는 천호지에서 fluorescent in situ hybridization (FISH) 방법을 이용하여 세균군집구조를 조사하였다. 조사기간동안 총세균수는 0.6~$1.3{\times}10^7 \cells{\cdot}ml^-1$ 의 범위로 매우 높게 나타났다. 또, 총세균수에 대한 eubacterial group이 차지하는 비율의 경우 29.8~45.8%로 작은 분포를 차지하였다. 세균군집구조를 분석한 결과, $\alpha$-group은 1.4~l2.5%, $\beta$-group은 0.9~4.9%, $\gamma$-group은 0.6~8.3%, $\circledcirc$Cytophaga-Flavobacteruim group은 1.0~8.3%를 나타내어 본 연구에서 조사된 각 group이 차지하는 비율은 낮고 상대적으로 unknown eubacteria의 비율이 높았다. 이러한 군집구조는 일반적으로 다른 담수호에서 나타나는 세균의 군집구조와는 다른 군집구조 이며 특히, aggregates에 부착한 세균 군집구조와 비슷한 양상이었다. 또, cyanobacteria의 조성 이 Anabaena속에서 Microcystis속으로 천이가 일어난 시기에 Cytophaga-Flavobacteruim group이 크게 증가하는 것으로 나타나 세균군집의 크기는 식물플랑크톤의 영향을 받는 것으로 확인되었다. The composition of bacterial communities was detected in surface water of Cheonho Reservoir dominated by cyanobacteria, using fluorescent in situ hybridization (FISH) method. Total bacterial numbers were very high ranging from 0.6~$1.3{\times}10^7 \cells{\cdot}ml^-1$, whereas the ratio of Eubacteria to total bacteria was 29.8~45.8%, which was lower than that in other freshwater ecosystems. On average only 2.1% of DAPI-stained bacteria were detected by FISH with probes for $\alpha$, $\beta$, and $\gamma$-groups, respectively. Unknown eubacteria which was not bound to any probes except EUB 338, was relatively high. On the other hand, the Cytophaga-Flavobacterium group increased following the change of dominant species from Anabaena sp. to Microcystis sp. This result showed that bacterial communities could be affected by phytoplanktons, especially cyanobacteria.
카지노 인근에 머무는 중노년 여성의 도박과 체류 경험에 관한 연구
홍선희 한국여성심리학회 2019 한국심리학회지 여성 Vol.24 No.3
Women gamblers are marginalized and excluded from the society while being blamed and stigmatized. However, in the situation where the rate of women gamblers is increasing, the purpose of this study is to reveal and understand gambling experiences and everyday lives of women who stay near the casino through their own voices. The researcher stayed in the field for a while and conducted participatory observation and in-depth interview based on field surveys. Twenty middle-aged women gamblers of staying near the casino participated in the interviews. They have usually lived a (non)conforming life in the family, and were exposed to gambling in a life-threatening crisis of divorce or bereavement with husband, then became addicted to gambling by experiencing fun and pleasure. Despite the hard work near the casino to make money, they have wanted to settle there as members of the community. Therefore, this study suggests the need for social intervention to cope with the life crisis of women in their life cycle, and for support that women gamblers can return to and settle in the community.