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꾸지뽕잎 및 꾸지뽕 잎차에 함유된 1-deoxynojirimycin의 GC 분석
이진식(Jin Shik Lee),이승언(Sung En Lee) 한국유화학회 2009 한국응용과학기술학회지 Vol.27 No.3
1-Deoxynojirimycin(1-DNJ) was hardly detected by general UV detector. Therefore, in this study, we analyzed 1-DNJ, a effective functional material in which was contained the leaves of Cudrania tricuspidata and its tea by gas-chromatography. Synthesized a TMS derivative by attaching trimethylsilyl group to 1-DNJ, analyzed this by GC, and could detect a good peak. The leaves of Cudrania tricuspidata contains 1154.83 ± 56.67 ug/g.d.w of 1-DNJ and tea of Cudrania tricuspidata leaves does 8.01 ± 0.61 ug/g.d.w. This means the contents of 1-DNJ was nearly identical to each other. The larger size of the leaves had the more contents of 1-DNJ and the middle region of collection was the highest than any other collective regions of leaves.
한재숙,한경필,이진식,김영진,Han, Jae-Sook,Han, Gyeong-Phil,Lee, Jin-Shik,Kim, Young-Jin 동아시아식생활학회 2007 동아시아식생활학회지 Vol.17 No.4
The purpose of this study was to investigate the perception of Korean kimchi in Singapore. A questionnaire was given to male 236(43.0%) and female 313(57.0%) college students residing in Singapore. The results are as follows: 86.9% of the participants answered that kimchi is Korean in origin, and 48.7% had eaten kimchi. Also 75.2% of the males and 65.2% of the females had purchased commercial kimchi at restaurants(p<0.05). Regarding the primary reason they purchased commercial kimchi, 62.1% responded 'its taste'(male 58.1%, female 65.0%), also 47.6% stated the package size was 50g(male 44.8%, female 49.7%). Among the kimchi they had experienced, 86.7% had eaten Baechu kimchi, 31.0% Mu kimchi and 30.9% Oi kimchi(males : 82.2% Baechu kimchi, 30.9% Oi kimchi, and 27.4% Mu kimchi, females: 90.0% Baechu kimchi, 33.6% Mu kimchi, and 26.2% Oi kimchi respectively). For their kimchi preference, 67.0% preferred Baechu kimchi, 9.4% Oi kimchi, and 7.1% Mu kimchi(males: 63.4% Baechu kimchi, 12.9% Oi kimchi and 5.4% Mu kimchi, females: 69.5% Baechu kimchi, 8.4% Oi kimchi and 6.9% Mu kimchi, respectively). After having eaten kimchi, 19.3% answer it tasted good, and the primary reason for liking kimchi, 'spicy and hot taste'(51.3%), The main reasons for not liking kimchi were the odor(garlic, ginger, anchovy juice, etc) and too spicy, respectively. Regarding improvements for its expanded consumption 32.0% answered 'not to improve', 18.0% answered 'don't make it too hot', 17.6% answered 'don't make it too salty', and 9.4% answered 'don't make it over-ripe'. For the overall perception of kimchi, the answer with the highest mean(3.95) was 'kimchi is a good side dish with cooked rice', which was significantly different than 'kimchi is delicious'(M=3.14, p<0.05).
체세포의 공배양체계에서 단일 에너지원이 인간 배반포기 배의 형성에 미치는 영향
박기상,최인경,이진식,송해범,Park, Kee-Sang,Choi, In-Kyung,Lee, Jin-Shik,Song, Hai-Bum 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.1
This study was conducted to investigate the effects of Tissue Culture Medium 199 (TCM) and Dulecco's Modified Eagle Medium (DMEM) on the blastulation and grade of human oocytes on Vero cells in vitro. A cohort of 79 and 93 oocytes in metaphase II stage were used in TCM 199 and DMEM respectively. No differences were found in the numer of oocytes showing two-pronuclei between TCM (82.3%) and DMEM (86.0%). The number of fertilized oocytes reaching the blastocyst was not significant in TCM (60.0%) and DMEM (63.1%). A total of 89 blastocysts were categorized into the four grades (BG1, BG2, BG3 and early) depending on their morphology. The number of embryos achieving the blastocyst grade 1 (BG1) was significantly higher (p<0.05) in DMEM (50.8%) than TCM (15.0%). It is concluded that cultured oocytes in DMEM with glutamine on Vero cells should be significantly increased BG1.
이병대 ( Byung Dae Lee ),이진식 ( Jin Shik Lee ),김영찬 ( Yeoung Chan Kim ) 한국유화학회 2004 한국응용과학기술학회지 Vol.21 No.4
N/A Ethanol washing with distillation as a cleanup process of polycyclic aromatic hydrocarbon(PAH)-contaminated soil was investigated in this study. A multistage ethanol washing with distillation process was applied to three different types of soil, i.e., sandy soil, alluvial soil, and clay with the initial concentration of benzo(a)pyrene 10 ㎎/㎏, benz(a)anthracene 250 ㎎/㎏, and pyrene 100 ㎎/㎏ soil. Ethanol was selected as washing solvent because of its high PAH removal efficiency, low cost, and non-toxicity comparing to the other solvent such as isopropyl alcohol and sodium dodecyl sulfate. The satisfactory results (i.e. lower than benzo(a)pyrene 1 ㎎/㎏, pyrene 10 ㎎/㎏, benz(a)anthracene 25 ㎎/㎏, which are the Canada or the Netherlands soil standard) for three types of soils were obtained by at most five-six times washing. It was suggested that organic content in soil decreased the removal efficiency by ethanol washing.