태아시기의 방사선 노출에 의한 마우스 뇌 발달에 미치는 영향에 관한 형태학적 연구

이종환,오헌,김세라,이해준,김태환,이윤실,김성호,Lee, Jong-hwan,Oh, Heon,Kim, Se-ra,Lee, Hae-june,Kim, Tae-hwan,Lee, Yun-sil,Kim, Sung-ho 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3

The present study analyze the morphological aspects of the cerebrum of mice with prenatal exposure to high and low dose (0.5, 1, 2 Gy) of $\gamma$-radiation on gestation day 12 or 16. The animal were allowed to give birth and the offspring were sacrificed at postnatal days 28 for gross and microscopic examination of cerebrum. Their body weight, brain weight, brain length, brain width, cortical thickness and area of cingulum bundle were examined. The histological and planimetric analysis were performed observing coronal sections. The gross malfomation (microcephaly) and abnormality of cortical architecture were prominent after exposure to 2 Gy on day 12 of gestation. significant dose-related reductions in body weight, brain weight, brain size were found in all irradiated groups. A significant change was found in thickness of the cerebral cortex and area of the cingulum bundle in the groups exposed to 0.5 Gy or more. There was no difference a lamina patter of six layers in cerebral cortex between the control and irradiated groups, but cell packing density increased significantly in the group exposed to 1 Gy or more. These results suggested that dose as low as 0.5 Gy could cause a morphologically reduce change in developing mouse cerebrum and exposure on day 12 of gestation to $\gamma$-irradiation is a particularly sensitive phase in causing malformation and abnormality of central nerve system.

전력비 변화에 따른 Au Multilayer 위에 증착한 GZOB 박막의 특성

이종환,유현규,이규일,이태용,강현일,김응권,송준태,Lee, Jong-Hwan,Yu, Hyun-Kyu,Lee, Kyu-Il,Lee, Tae-Yong,Kang, Hyun-Il,Kim, Eung-Kwon,Song, Joon-Tae 한국전기전자재료학회 2008 전기전자재료학회논문지 Vol.21 No.11

We investigated the effects of power ratio on the electrical and optical properties of Au based Ga-, B- codoped ZnO(GZOB) thin films. GZOB thin films were deposited on Au based poly carbonate(PC) substrate with various power in the range from 60 to 120 W by DC magnetron sputtering. In the result, GZOB films at 100 W exhibited a low resistivity value of $1.12\times10^{-3}\Omega-cm$, and a visible transmission of 80 % with a thickness of 300 nm. This result indicated that the addition of Ga and B in ZnO films leads to the improvement of conductivity and transparent. From the result, we can confirm the possibility of the application as transparent conductive electrodes.

혼합현실 환경에서 레가시 데이터를 활용하는 가상 공정배치 시뮬레이션 시스템

이종환,신수철,한순흥,Lee, Jong-Hwan,Shin, Su-Chul,Han, Soon-Hung 한국정보처리학회 2009 정보처리학회논문지 A Vol.16 No.6

디지털 가상 제조는, 생산 시스템의 요소를 컴퓨터상에서 모델링 하여 디지털 모델을 구성한 뒤, 3차원 CAD, 시뮬레이션 등의 관련 기술을 이용하여, 신속하고 효율적인 제품 개발과 생산 프로세스를 사전에 검증하여 지원하는 기술이다. 여기에 혼합현실 기술을 응용하면, 모든 개체 가 그래픽 객체들로만 표현된 순수한 가상환경 기반의 디지털 가상 제조 기술을 보완하여 좀 더 현실감이 있는 환경을 제공할 수 있으며, 동시 에 필요한 일부만 그래픽 객체로 표현하므로 모델링에 필요한 시간과 비용을 절감할 수 있다. 본 논문은 이러한 혼합현실 기술을 디지털 가상 제조 과정 중 가상 공정설비 배치 기술에 이용하면서, 현장에 이미 구축되어 있는 레가시(legacy) 데이터를 이용하는 방안을 제안한다. 이를 위 하여 혼합현실 기반 공정설비 배치 시스템에 적합한 현장의 레가시 데이터의 취득 방법과 가공 방법이 개발되었으며, 가공된 데이터를 기반으 로 시뮬레이션이 가능한 혼합현실 기반 디스플레이 시스템을 구축하였다. 개발된 혼합현실 기반 가상 공정설비 배치 시스템은, 실제 설비를 배 치하기 전에, 현장에서 그 위치와 적용성을 미리 검증함으로써, 설비 배치와 관련된 오류를 줄일 수 있다. Digital virtual manufacturing is a technology that aims for the rapid development of products and the verification of production-process in ways that are more efficient by integrating digital models within the entire manufacturing process. These digital models utilize various information technologies, such as 3D CAD and simulations. Mixed reality, which represents graphical objects for only needed parts against real scene, can bring a more enriched sense of reality to an existing virtual manufacturing system that is in a pure virtual environment, and it can reduce the time and money needed for modeling the environment. This paper suggests a method for planning virtual factory layouts based on mixed reality using legacy datathat are already constructed in the real field. To do this, we developed the method to acquire simulation data from legacy data and process this acquired data for visualization based on mixed reality. And then we construct display system based on mixed reality, which can simulate virtual factory layout with processed data. Developed system can reduce errors related with factory layout by verifying the location and application of equipments in advance before arrangement of real ones at the practical job site.

고병원성 조류인플루엔자(HPAI) 현장 방역

이종환,Lee, Jong-Hwan 대한수의사회 2008 대한수의사회지 Vol.44 No.11

이번 고병원성조류인플루엔자(이하 HPAI로 기술)는 2008년 4월 1일 우리 전라북도 김제시 용지면 산란계 밀집 사육지역에서 시작하여 5월 12일 경북 경산시 토종닭 농장을 끝으로 11개 시 도 19개 시 군 구에서 총 33건이 발생되어 2,6410억원의 경제적 피해가 발생하였다. 우리 전라북도는 최초 발생농가를 포함 4개 시 군(익산, 정읍, 김제, 순창)에서 17건(닭13, 오리 4)이 발생하여 전국에서 HPAI발생이 가장 많았던 도로 남는 불명예를 안게 되었다. 이에 막대한 경제적 피해가 다시 발생하지 않도록 HPAI의 현장 방역에 대해 나름대로 경험한 것과 느꼈던 것들을 사례별로 기술하고자 한다.

변증록(辨證錄) 권지십일(卷之十一)에 대(對)한 연구(硏究)

이종환,김주태,김상운,엄동명,이시형,Lee, Jong-Hwan,Kim, Joo-Tae,Kim, Sang-Un,Eom, Dong-Myung,Lee, Si-Hyeong 대한한의정보학회 2011 大韓韓醫情報學會誌 Vol.17 No.1

The topic of this study is the translation of "Byun Jeung Rok Vol.XI(辨證錄十一卷)". Byun Jeung Rok is a complete book of medicine which was written by Jin Sa tak(陣士鐸) in the early years of the Ching dynasty. Of this, the eleventh volume is composed of 6 disease-patterns(病證) and 41 subtopics. The organized characteristics of "Byun Jeung Rok(辨證錄) Vol.XI" is as follows. Chapter 1 refers to the symtom, agent and prescription of Hysterorrhea(帶下病). It explains five color Hysterorrhea; white, red, black, yellow and blue. Chapter 2 refers to the symtom, agent and prescription of Oligemic Menoschesis(血枯). It explains two cases of Menoschesis. Chapter 3 refers to the symtom, agent and prescription of Metrorrhagia(血崩). It explains eight cases of Metrorrhagia. Chapter 4 refers to the symtom, agent and prescription of Dysmenorrhea(月經不調). It explains fourteen cases of Dysmenorrhea. Chapter 5 refers to the symtom, agent and prescription of Infertilitas(不姙). It explains ten cases of Infertilitas. Chapter 6 refers to the symtom, agent and prescription of Hyperemesis(惡阻). It explains two cases of Hyperemesis. Obviously the Byun Jeung Rok(辨證錄) is one of the very important texts in the oriental medicine. But there is no exact Korean translation, so I study this six chapters and translate them into Korean. Finally, I hope this thesis can be an aid of development in the knowledge informatization of Korean medicine.

Role of Rho A and F-actin for uropod formation in T lymphocytes

이종환,Lee, Jong-Hwan Korean Society of Life Science 2007 생명과학회지 Vol.17 No.2

외부 병원체 침입으로 이동하고 있는 T 세포는 두가지 뚜렷한 형태적인 변화, 즉, leading edge와 uropod를 형성하여 효과적으로 T세포 이동에 영향을 미친다. Uropod 구조물은 이동하는 림프구들의 뒤쪽에서 관찰 할 수 있는 아주 독특한 구조로 CD44, ERM, F-actin과 같은 단백질들이 서로 영향을 미치며 모인다. F-actin cytoskeleton은 세포의 형태를 유지하는 기본적인 틀을 제공한다. Rho A small GTPase는 이러한 cytoskeleton을 재구성하는 organizer로 역할을 한다고 보고되어 왔다. 지금까지, 다양한 경로를 통하여 Rho A가 활성화 되어 진다고 보고 되었다. 본 실험에서 PDZ 도메인이 세포 내부 RHo A에 GDP가 결합된 불활성화 형태의 Rho A를 GTP가 결합된 활성화 형태로 전환한다는 것을 알았고, F-actin cytoskeleton을 재구성 하며, PDZ 도메인을 함유한 세포는 uorpod 구조물이 없어졌으며 세포 이동 속도도 감소하는 것을 알았다. 따라서 Rho A와 F-actin cytoskeleton 사이의 신호 전달 과정이 uropod 형성에 아주 중요한 기능을 할 것이라는 것을 알았다. Two distinct morphological features, leading edge and uropod, in mobile T lymphocyte are crucial for efficient directional movement. The uropod is a unique rear protrusion in migrating lymphocytes, in which several proteins, including CD44, ERM (ezrin/radixin/moesin), and F-actin cytoskeleton are concentrated and concerted. F-actin cytoskeleton is a basic mold for the shape maintenance. Rho A small GTPase acts as cytoskeleton organizer, So far, various pathways potentially can induce the Rho activation. PDZ domain is able to increase active Rho A form (Rho-GTP) level, reorganize F-actin cytoskeleton, disrupts the uropod structure and cell migration was diminished, suggesting that signaling pathways between Rho and F-artin cytoskeleton are related to uropod formation.

경비업법 제7조 제1항 "관리권 범위"에 대한 법적 해석

이종환,이민형,Lee, Jong-Hwan,Lee, Min-Hyung 한국경호경비학회 2011 시큐리티연구 Vol.- No.26

Security Business Act of Korea is leaning toward the aspect of regulation, while it provides few provisions about vesting the authority, which causes problems such as legal loopholes to be raised in regard to the rules about authorizing the private security personnel to perform security affairs properly and defining the limit of power. So, it should be done to interpret the law in order to draw the legal basis of empowering as well as to set limits of the authority of security main agent, and Article 7, Section 1, of Security Business Act is the very provision that involves the legal basis. In the scope of 'Management Power', the statutory authority in the first clause of Article 7 of Security Business Act, the security personnel can use force for self-defense, defense of others and property, and prevention of crimes. In addition, the powers of interrogation, access control, and eviction notice are involved in its scope. The private security personnel as the occupation assistant can take precautions and if the infringement on the benefit and protection of the law is imminent or done, he or she can use force within the limits of the passive resistance and the means of defiance on the basis of 'Management Power'. The private security personnel, however, can exercise the force only if the necessary conditions of legal defense, emergency evacuation, and legitimate act of criminal law are fulfilled. 경비업법의 주요 내용을 살펴보면, 경비업에 대한 규제적 측면에 치우쳐 있으며, 구체적인 권한 부여에 관한 규정이 명확히 제시되어 있지 않다. 이것은 경비업무을 실질적으로 수행하고 있는 경비원의 권한에 대한 근거 규정의 미비와 그러한 권한의 한계를 명확히 설정하지 못하는 문제를 야기하게 된다. 이에 따라 경비업법상 경비업 주체의 업무 수행의 한계를 부여함과 동시에 경비업무의 원활한 수행을 담보할 수 있는 권한 근거 규정을 도출할 수 있는 해석이 이루어져야 하며, 그러한 단서를 제공하는 것이 경비업법 제7조 제1항의 "관리권"이라 할 수 있다. 경비업법에 규정된 관리권의 범위에는 점유권에 기한 자력구제권을 근거로 하여 자력방위 및 자력탈환권이 포함되며, 사실적 지배행위로서 정지 및 질문권, 출입통제 및 퇴거요구권, 물품 검색 반입 보류 및 금지권 등이 인정된다고 할 것이다. 점유보조자의 지위에서 경비원은 관리권으로부터 도출될 수 있는 사전예방 활동을 할 수 있으며, 이러한 예방활동에도 불구하고 법익 침해가 예상되거나 이루어진 경우 관리권에 근거하여, 소극적 저항행위와 저항수단으로서의 유형력 범위 안에서 실력으로 침해를 저지 할 수 있다. 그리고 그러한 물리적 유형력을 행사하기 위해서는 형법상 위법성조각사유인 정당방위 긴급피난 정당행위의 요건을 구비하여야 한다.

Differential Expression of Chemokine MCP-1, MIP-1α, MIP-2 in Lipopolysaccharide-stimulated Neonatal and Adult Rat Brain

이종환,Lee, Jong-Hwan Korean Society of Life Science 2006 생명과학회지 Vol.16 No.5

외부 병원체의 침입에 의한 병원독소로 발병된 뇌염증에서 미성숙뇌는 성숙뇌에서 보다 많은 백혈구의 침윤을 일으킨다. 케모카인은 뇌염증부위로 염증세포의 침윤을 매개하는 물질이다. 본 연구는 미성숙뇌의 뇌염증에서 백혈구침윤의 기전을 연구하기 위하여 내독소로 유발된 뇌염증에서 케모카인의 일종인 MCP-1, $MIP-1{\alpha}$, MIP-2의 발현을 연구하였다. 신생쥐와 성숙쥐의 미상핵 국소에 LPS $(0.5\;{\mu}g/{\mu}l)$를 정위주사 한 후 시간대별로 RT-PCR과 면역조직화학검사을 통하여 각각 mRNA상과 단백질상에서의 발현을 비교분석하였다. 광학현미경상 LPS 주입 후 6 시간 후부터 주사부위의 염증세포의 침윤이 시작되었으며 24 시간 후에 가장 뚜렷한 현상을 보였다. RT-PCR 결과, MCP-1, $MIP-1{\alpha}$, MIP-2 mRNA 발현은 24 시간 때에 최고치를 나타냈었다. 미성숙뇌의 각 케모카인의 mRNA발현은 성숙뇌에 비해 MCP-1은 약 2.6배, $MIP-1{\alpha}$는 약 1.4배, MIP-2는 약 1.2배 발현양이 더 많다. 면역조직화학검사는 광학현미경결과와 RT-PCR결과와 상응하여 각 케모카인들이 24 시간 때에 가장 양성반응이 뚜렷이 나타났다. 이러한 현상은 뇌염증에서 백혈구의 침윤은 케모카인을 생성하는 소교세포, 성상세포, 내피세포 등의 영향을 받는 기전에 의하여 조절되었다는 것을 나타내며 이들 케모카인 형성환경의 역할을 명확히 밝히고 질병의 진행에서 케모카인의 활성을 조정하는 방법을 연구하면 향후 뇌염증을 포함한 여러 가지질환에서 신경세포의 손상을 막는 치료법의 개발이 가능하게 될 것이다. Severe brain injuries induced by toxin pose one of the most important problems on our health care because of their high morbidity and mortality, are implicated to leucocyte infiltration more premature or immature brain than mature brain. Chemokines are the induction meditators for infiltration of inflammatory cells to the inflammation sites. In order to study the mechanism of leucocyte infiltration, the expression of several chemokines, MCP-1, $MIP-1{\alpha}$ and MIP-2 was studied in lipopolysaccharide(LPS)-stimulated neonatal and adult brain. One week old Sprague-Dawley rats or adult male rats weighing 300-350 g were used for the experiment. After anesthetization, $1\;{\mu}l$ LPS (0.5 mg/ml) subsequently was injected in the right caudate nucleus of the brain with stereotaxic frame. Animals were sacrificed at 6 hours, 24 hours, and 72 hours after injection. The present study was carried out using RT-PCR for the mRNA and immunohistochemistry for the expression of the proteins. In the neonatal rat brain, prominent interstitial edema with significant accumulation of leukocytes was detected at 24 and 72 hours after LPS injection. A semiquantitative analysis of RT-PCR revealed that the MCP-1, $MIP-1{\alpha}$, and MIP-2 mRNA expression peaked at 24 hours in neonatal and adult rat brain. Neonatal rats showed about 2.6, 1.4, and 1.2 times more expression of the MCP-1, $MIP-1{\alpha}$, and MIP-2 than that of the adult rats in the brain tissue. Immunohistochemical analysis also showed that MCP-1 immunoreactivity was paralleled with the RT-PCR results. MCP-1 protein was significantly detected at 24 and 72 hours in the brain parenchyma. $MIP-1{\alpha}$protein was highly expressed at 24 hours. The results of leukocyte infiltration in H&E stain was parallelled with that of the immunohistochemistry. Chemokine proteins were markedly detected at 24 hours after injection of LPS and neutrophil influx into intraparenchymal was prominent at 24 hours. These results suggest that the leukocyte infiltration in the intracranial infection may be controlled by mechanisms influenced by chemokine producing cells in the central nervous system such as microglia, astrocyte and endothelial cell.

한국재래산양의 태아 및 신생아의 제4위 발달에 관하여

이종환,허찬권,김종섭,곽수동,Lee, Jong-hwan,Huh, Chan-kwen,Kim, Chong-sup,Kwak, Soo-dong 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.2

The development of abomasum in fetuses between 60, 90, 120 days of gestation and neonates of Korean native goats was investigated by light, scanning and transmission electron microscopy. The results obtained were summarized as follows ; 1. The abomasum wall appeared to be differentiated into the epithelium, lamina propria, muscle layer, and serosa at 60 days of gestation. The epithelium was stratified columnar and these nuclei were located near the apical two thirds portion of the cell at 60 days of gestation, and then transformed into simple columnar epithelium with the flat basal nuclei. 2. The inner circular and outer longitudinal muscle layers were observed at 90 days of gestation and the blood vessels had become quite well developed as various arterioles, venules and capillaries of different size during this age. 3. Gastric pits were seen at 90 days of gestation and continued gradually to increase depth during gestation. 4. The mucous, parietal and chief cells appeared in epithelium at 90 days of gestation and continued gradually to increase in number during gestation. In 120 days fetuses and neonates, muscle layer had become very thickeness. 5. Scanning electron microscopically, the inner surface of the abomasum already consisted of wavy spiral folds which had many fine wrinkles at 60 days of gestation. In 90 day old fetuses, each spiral fold was enlarged and its surface was tended to be split into many straight longitudinal ridges and among these ridge were found shallow grooves, At 120 days, the subdivided swellings of ridges were progressively complicated in shape. In the neonates, the inner surface was flat and holed with many gastric pits. 6. Transmission electron microscopically, the epithelium was straified columnar and these nuclei were irregular shape at 60 days fetus. The parietal, chief and mucous cells were observed in 90 day old fetuses and continued gradually to increase in number during gestation. 7. The development of the abomasum was relatively slow at early stages, it was accelerated greatly in the last of gestation.

Special Issues - NDIS 기반의 Mobile 배전설계시스템 개발 및 활용

이종환,Lee, Jong-Hwan 대한전기협회 2010 전기저널 Vol.- No.407