고슴도치 위점막의 내분비세포에 관한 면역조직화학적 연구

이재현,이형식,이남수,김종범,Lee, Jae-hyun,Lee, Hyeung-sik,Lee, Nam-soo,Kim, Jong-beom 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.1

한국산 고슴도치의 위점막에 분포하는 내분비세포에 대해 면역조직화학적으로 관찰하였던바, gastrin, somatostatin, 5-HT, glucagon, BPP, motilin, GIP 등 7종의 면역반응세포들이 동정되었으며, 이들 세포들의 부위별 분포와 출현빈도의 특정은 다음과 같다. Gastrin 면역반웅세포는 유문부에서만 아주 다수로 출현하였으며, somatostatin 면역반응세포는 유문부에서 다수, 분문부와 위저부에서는 중등도의 출현올 볼 수 있었다. 5-HT 면역반응세포는 분문부와 유문부에서 다수, 위저부에서는 중등도로 출현하였다. glucagon 면역반응세포는 위저부에서 소수로 그리고 유문부에서 극히 소수로 출현하였고, BPP 면역반응세포는 유문부에 다수로, 위저부에 중등도로 분문부에 소수로 분포하였다. motilin 면역반응세포는 유문부와 위저부에서 소수 또는 극소수로 출현하였으며, GIP 면역반응세포는 유문부에서만 소수의 분포를 볼 수 있었다. The gastric endocrine cells of the Korean hedgehog, Erinaceus korean us were studied immunohistochemically. Seven kinds of endocrine cells-, gastrin-, somatostatin-, 5-HT-, glucagon-, BPP-, motiIin-and GIP-immunoreactive cells- were identified in this study. The chracteristic findings of the regional distribution and relative frequency of them were examined. Gastrin-immunoreactive cells were very numerously detected only in the pyloric region. Somatostatin-immunoreactive cells were more numerous in the pyloric region than in the cardiac and fundic regions. 5-HT-immunoreactive cells were more numerous in the cardiac and pyloric regions than in the tundic one. Glucagon-immunoreactive cells were found few or rarely in the fundic and pyloric regions. BPP-imunoreactive cells were numerously distributed in the pyloric region, moderately in the fundic region and few in the cardiac region. Motilin-immunoreactive cells were found rarely or few in the fundic and pyloric regions. GIP-immnuoreactive Cells were detected onIy in the pyloric region.

한국산 고슴도치 분문부와 위저부의 내분비세포에 관한 전자현미경적 연구

이재현,이형식,이남수,김종범,Lee, Jae-hyun,Lee, Hyeung-sik,Lee, Nam-soo,Kim, Jong-beom The Korean Society of Veterinary Science 1990 大韓獸醫學會誌 Vol.30 No.4

한국산 고슴도치의 분문부와 위저부에 있어서 내분비세포를 전자현미경적으로 관찰하였던 바 EC, ECL, $D_1$, G 및 A-1ike 세포 등 5종의 세포가 동정되었다. EC 세포는 높은 전자밀도의 다형태성 과립을 가지며, 과립내에는 더 높은 천자밀도의 dense body가 보였고, 과립의 크기는 170~500nm였다. ECL 세포는 원형 또는 난원형의 과립을 가지며, 과립 내용물은 전자밀도가 높고 편재하거나 공포상으로 출현하였고, 과립의 크기는 220~450nm였다. $D_1$ 세포는 원형의 과립을 가지며, 과립의 전자밀도는 낮거나 중등도였고, 과립의 크기는 140~400nm였다. G 세포는 원형 또는 난원형의 과립을 가지며, 중등도의 전자밀도를 나다내는 과립은 내용물과 한계막 사이에 좁은 halo를 보였고, 과립의 크기는 200~400nm였다. A-like 세포는 원형의 과립을 가지며, 이들 과립은 높은 전자밀도를 나타내었고, 내용물과 한계막은 밀착하였으며, 과립의 크기는 170~260nm였다. Endocrine cells in the cardiac and fundic regions of the Korean hedgehog were studied ultrastructurally. Five types of endocrine cells classified as EC, ECL, $D_1$. G and A-like cells were identified in these regions. EC cells contained pleomorphic granules, 170~500nm in diameter, with high electron density and highly dense bodies in a dense matrix. ECL cells were characterized by the presence of round or oval granules, 220~450nm in diameter, with high electron density. Some granules of ECL cells showed a small amounts of contents or empty. $D_1$ cells contained round and relatively small granules, 140~400nm in diameter, with low to moderate electron density. G cells were characterized by the presence of round or oval granules, 200~400nm in diameter, with moderate electron density. Some granules of these cells showed a narrow halo between the limiting membrane and the granular matrix. A-like cells contained round granules, 170~260nm in diameter, With high electron density. The granules of these cells showed homogeneous matrix surrounded by the tight membrane.

과도한 마모를 보이는 환자에서, 임플란트를 활용해 수직 고경을 증가시키며 shortened dental arch 개념을 적용한 보철 수복 증례

이재현,김태수,이철원,이원섭,이수영,Lee, Jae Hyun,Kim, Tae Su,Lee, Chul Won,Lee, Won Sup,Lee, Su Young 대한치과보철학회 2016 대한치과보철학회지 Vol.54 No.1

과도한 마모 같은 잔존 치아 구조의 파괴는 전치유도와 저작 기능에 심각한 손상을 초래할 수 있다. 이런 증상을 개선시키기 위해서는 상실된 구치부를 수복하여 적절한 교합을 회복시켜주는 것이 필수적이다. 구치부의 무치악부를 수복하기 위해서는 의치나 임플란트가 사용될 수 있는데, 임플란트로 수복시에는 경제적인 제약으로 위해 shortened dental arch 개념을 적용하는 것이 유리할 수 있다. 본 증례는 86세 남환으로, 다수의 치아가 상실되어 있었고, 구치부 교합의 붕괴와 과도한 치질 소실, 수직 피개를 관찰할 수 있었다. 구치부에서 붕괴된 교합을 재형성해주는 것을 최우선 과제로 정하였으며, 가철성 보철물 대신, 국민건강보험이 적용된 2개의 임플란트와 고정성 국소의치, 레진을 이용하여 보철 수복을 완료하였다. 소구치 부위에서 2 mm의 수직고경을 거상하였으며, shortened dental arch 개념을 적용하였다. 이를 통해 전악 수복에 비하여 적은 비용으로 이를 대체하는 치료를 고정성 보철물로 할 수 있었으며, 심미적, 기능적으로 양호한 결과를 얻었다. Excessive occlusal wear can result in unacceptable damage to the residual teeth structure, anterior guidance and masticatory function. An 86-year-old man presented with worn dentition and anterior deep bite due to loss of the posterior support. Two implants covered by Korean national health insurance, fixed partial denture and direct composite resin were used to restore the dentition, instead of removable prosthesis. The occlusal vertical dimension was increased by 2 mm in the premolar area and shortened dental arch concept was applied. This treatment was a less expensive alternative to full-mouth rehabilitation with positive esthetic and functional outcomes after 7 months of follow-up.

COVID-19가 건설프로젝트 리스크에 미치는 영향

이재현,이성현,이동훈,Lee, Jae-Hyun,Lee, Seong-Hyeon,Lee, Donghoon 한국건설안전학회 2021 한국건설안전학회 논문집 Vol.4 No.1

현재 유행하고 있는 COVID-19는 '세계보건기구' 설립 이래 가장 많은 사망자가 발생한 대유행이다. 수많은 건설 프로젝트에도 상당한 영향을 미칠 것으로 예상된다. COVID-19가 건설업계를 강타한 이후, 그들이 감당해야 할 위험은 매년 줄어들었다. 그러나 COVID-19의 장기화로 인해 대기 지연, 자재 공급, 경제적 손실의 위험이 증가했다. 정확한 대책은 파악이 필요할 것이며 현재 건설사업 중 리스크가 더 큰 비중으로 완화될 수 있어야 한다. 따라서 이번 연구는 COVID-19로 인해 국내 건설사에 건설사업에 영향을 미친 위험요소를 분석하고 규명하는 것을 목적으로 한다. 이전 건설 프로젝트의 리스크를 바탕으로 조사, 가중치 부여 등을 통해 리스크 사례 연구, COVID-19 관련 리스크 등을 추출한다. 각 위험 요소는 AHP 분석 기법을 기반으로 계산된다. 이에 따라 COVID-19로 인해 건설공사 리스크 연구 결과가 달라질 것으로 예상된다. 그것은 현재 상황과 이후의 전염병 상황에 대처하기 위해 제시될 것이다. COVID-19, which is currently in vogue, is a pandemic with the largest number of deaths since the establishment of the "World Health Organization". It is also expected to have a significant impact on countless construction projects. After COVID-19 hit the construction industry, the risk that they needed to cover, decreased every year. However, the prolonged COVID-19 increased the risks of air delays, material supply, and economic losses. The exact measurements will be needed to be identified and the risks of the current construction projects must have a mitigated risk with a greater proportion. Therefore, the purpose of this study is to analyze and identify the risks that have influenced construction projects to the domestic construction companies due to COVID-19. Based on the risks of the previous construction projects, risk case studies, and risks related to COVID-19, are extracted through surveys, weights. Each risk factor are calculated based on the AHP analysis technique. Thus, it is expected that the results of the risk research on construction projects will change due to COVID-19. It will be presented to cope with the current situation and later pandemic situations.

두툽상어 췌장에 출현하는 내분비세포의 면역조직화학적 연구

이재현,이남수,이형식,김종법,Lee, Jae-hyun,Lee, Nam-soo,Lee, Hyeung-sik,Kim, Jong-beom The Korean Society of Veterinary Science 1991 大韓獸醫學會誌 Vol.31 No.1

두툴상어의 췌장 내분비세포를 면역조직화학적으로 관찰하였던바, 5종의 면역반응세포들이 동정되었다. glucagon 면역반응세포는 도관의 상피간 및 췌장외분비선의 선포주위에서 한개 또는 다수의 집단으로 출현하였으며, insulin 면역반응세포는 islet의 주변부 또는 도관의 상피간에 각각 관찰되었다. somatostatin 면역반응세포는 도관의 상피간 또는 췌장외분비선의 주위에 한개 또는 집단으로 분포하였으며, 소수의 5-HT 연역반응세포는 islet의 주변부 및 췌장 외분비선의 선세포간에서 관찰되었다. BPP 면역반응세포는 극소수로 관찰되었으며, GAS/CCK외 chromogranin 면역반응세포들은 관찰되지 않았다. The pancreatic endocrine cells of the cat-shark, S. torazame, were studied using immunohistochemical method. Five kinds of endocrine cells (glucagon-, somatostatin-, insulin-, 5-HT-and BPP-immunoreactive cells) identified in this study. The chracteristic findings of the distributions of five immunoreactive cells were as follows. Glucagon-immunoreactive cells were detected as clustering group in the epithelia of the interlobular duct and singly the pancreatic acini, respectively. Insulin -immunoreactive cells were moderately observed in the epithelia of the interlobular duct or in the periphery of the islet. Somatostatin-immunoreactive cells were distributed in single or mass groups in the epithelia of the interlobular duct and the exocrine gland of the pancreas. A very few 5-HTimmunoreactive cells were seen in the periphery of the islet and the acini of the pancreas-BPP-immunoreactive cell was singly located in the periphery of the pancreatic islet, but GAS/CCK-and Chromogranin-immunoreactive cells were not found in this study.

Ultrastructural Study on the Luminal Epithelium of the Ovariectomized Rat Uterus after Hormonal Treatment

이재현,이헌주,Lee, J.H.,Lee, H.J. Korean Society of Electron Microscopy 1984 Applied microscopy Vol.14 No.2

난소절제한 흰쥐에서 $17{\beta}$-estradiol과 progesterone을 장기간 주사햐여 자궁상피의 형태적 변화를 전자현미경으로 관찰하였다. 난소절제 후 자궁상피는 막성구조물과 지방구 기타의 구조물을 포함하는 vacuole의 출현이 특이적으로 나타났다. 상피는 낮은 입방형이며 세포 유리면에 소수의 짧은 microvilli를 볼 수 있었다. Estradiol투여시 상피는 높은 윈주형을 나타내며, 분비계는 비교적 잘 발달되고, 세포 유리면에 소수의 긴 microvilli도 관찰되었다. Progesterone 처리시의 상피는 세포 첨단부에 다수의 vacuole과 핵하부에 다수의 지방구의 집적이 관찰되었다. 이상의 결과로 보아 자궁상피는 형태학적 및 기능적인 상태에서 estrogen과 Progesterone 양자에 의해 변화를 받으며 이들 호르몬은 상피세포에 대해 독특한 영향을 나타낸다고 생각된다. Morphological changes of the epithelium of the endometrium by prolonged treatment of $17{\beta}$-estradiol or progesterone in ovariectomized rats was studied at the ultrastructural level. The epithelium of the endometrium in ovariectomized rats was characterized by the appearance of a number of vacuoles which was contained with the membraneous structures, lipid droplets and the others. The epithelium was low cuboidal, and a few short microvilli were present at the cell surface. Secretory granules are rarely found. After estradiol treatment, the epithelium was high columnar in shape. The mitochondria was appeared throughout the cytoplasm, however, long or swelling mitochondria was often found. Golgi apparatus and rER were relatively well-developed. Relatively long and sparse microvilli were present at the cell surface. After progesterone treatment, the epithelium was characterized by the appearance of numerous vesicles at the apical region and numerous lipid droplets at the subnuclear region. At the cell surface a number of short and blunt microvilli were found. These data indicated that the endometrium was dependent on estrogen and progesterone for changes in both its morphological and functional state and suggested that each hormone exerted a unique effect on the epithelial cells.

한국재래산양 췌장 내분비세포의 면역전자현미경적 연구 : bovine pancreatic polypeptide와 chromogranin의 공존

이재현,Lee, Jae-Hyun 한국현미경학회 1995 Applied microscopy Vol.25 No.1

Pancreatic endocrine cells of the native Korean goat were investigated immunocytochemically at electron microscopic level. All glucagon-, insulin-, somatostatin- and pancreatic polypeptide(PP)-immunoreactive cells were showed chromogranin(CG) immunoreactivity in the secretory granules of each cells. In addition, bovine pancreatic polypeptide immunoreactivity was found to be colocalized in the secretory granules of the glucagon and insulin cells. These observations support that chromogranin is available as the marker of pancreatic endocrine cells on the native Korean goat and BPP colocalized in the secretory granules of glucagon and insulin cells.

기러기 췌장 내분비세포에 대한 면역조직화학적 연구

이재현,구세광,이형식,Lee, Jae-hyun,Ku, Sae-kwang,Lee, Hyeung-sik 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

The regional distribution and relative frequency of the endocrine cells in the pancreas of the bean goose were investigated by immunohistochemical methods using 6 types of the specific antisera. Spindle shaped serotonin-immunoreactive cells were detected in the exocrine portions. Spherical or spindle shaped glucagon-immunoreactive cells were observed in the exocrine and dark and mammalian type islets. In the dark type islets, numerous cells were dispersed throughout whole islets but they were located in the peripheral regions of the mammalian type islets. No glucagon-immunoreactive cells were detected in light type islets. Round or spherical shaped insulin-immunoreactive cells were observed in the exocrine and dark, light and mammalian type islets. They were observed in the exocrine regions with a few numbers. Extremely rare cells were detected in central portion of the dark type islets but moderate to numerous cells were found in the central regions of the mammalian and light type islets, respectively. Spherical or spindle shaped somatostatin-immunoreactive cells were observed in the exocrine and dark, light and mammalian type islets. A few single cells were detected in the exocrine portions. In the dark type islets, numerous cells were dispersed throughout whole islets but a few to moderate numbers of cells were located in the peripheral regions of the light and mammalian type islets. Moderate numbers of the bovine pancreatic polypeptide-immunoreactive cells were found in the exocrine portions with round, spherical or spindle shape. But no bovine Sp-1/chromogranin-immunoreactive cells were observed in this study.

Ni기 초내열합금의 일방향 응고에서 탄화물의 Trapping과 성장 거동

이재현,진건,이용태,최승주,Lee, Jae-Hyeon,Jin, Geon,Lee, Yong-Tae,Choe, Seung-Ju 한국기계연구원 1996 硏究論文集 Vol.26 No.-

The carbide growth behavior of MAR-M247 LC alloy was investigated by directional solidification and quench method. The carbide volume fraction, trapping and growth behavior were correlated with the growth rate. It was found that the carbide volume fraction decreases at slower growth rate. This decreasing was caused by lower solid-liquid interface trapping ability at the slower growth rate.

산개구리 위장관 내분비세포의 전자현미경적 연구

이재현,이형식,Lee, Jae-hyun,Lee, Hyeung-sik 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.2

In the present paper, the distribution, relative frequences and cell types of endocrine cells in the gastrointestinal tract of the frog (Rana dybowskii) during the hibernating and the active phase were examined by light and electron microscopy. The results obtained are summarized as follow: The reactive cells for Grimelius were frequently found in the gastrointestinal tract, whereas the reactive cells for Hellman-$Hellerstr{\hat{o}}m$ were found numerous in the fundus and pylorus of stomach, a few in the duodenum and lower small intestine, and very few in the rectum during both phases. No reactive cells for Masson-Fontana were found in the gastrointestinal tract during both phases. Elecron microscopically, 4 types of endocrine cells in the fundus of the stomach, 3 types in the pylorus of the stomach and duodenum, and 1 type in the lower small intestine and rectum, respectively, were identified during the hibernating phase. In the active phase, 3 types of endocrine cell in the fundus of the stomach, 2 types in the pylorus of the stomach and duodenum, and 1 type in the lower small intestine and rectum were observed, respectively. In the hibernating phase, more cytoplasmic granules and various types of endocrine cells were generally found than in the active phase.