Glutathione S - transferase ρ의 정제 및 특성

이재용,한미영,김두식 ( Jae Yong Lee,Mi Young Han,Doo Sik Kim ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

Glutathione S-transferase ρ(EC 2.5.1.18) has been purified to homogeneity from human erythrocytes. A combination of gel filtration, ion exchange and hydroxylapatite chromatographic procedure yields the specific activity of 20.8 units/㎎. The purified enzyme gives a single band corresponding to 24,000 M.W. on a sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme molecule is characterized to be an acidic protein (pl 4.6) having a dimeric structure with 48,000 M.W. composed of identical size of polypeptide chains. Apparent K_m, and V_(max) were determined to be 1.1 mM and 1.0 mmol/1/min for 1-chloro-2,4-dinitro benzene respectively while 0.3 mM and 0.55 mmol/l/min for glutathione. Results obtained from chemical modification studies suggest that essential amino group(s) critically connected to the catalytic function of glutathione S-transferase ρ.

Glutathione S-Transferase ${\rho}$의 정제 및 특성

이재용,한미영,김두식,Lee, Jae-Yong,Han, Mi-Young,Kim, Doo-Sik 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

사람의 적혈구로부터 glutathione S-transferase $\rho$를 순수하게 분리 정제하였다. 황산암모늄 분별 침전에 이어 세단계의 크로마토그라피 분리과정을 거쳐 정제한 glutathione S-transferase $\rho$의 비활성도는 20.8 units/mg이었다. 정제된 효소단백질은 Sephadex G-100 gel filtration과 SDS-polyacrylamide gel 전기영동 분석결과에 의하여 subunit 분자량 24,000인 dimer 구조임을 확인하였으며 측정된 isoelectric pH는 4.6 이었다. 1-chloro-2, 4-dinitrobenzene에 대한 $K_m$, $V_{max}$는 각각 1.1 mM, 1.0mmol/l/min 였고 glutathione에 대해서는 0.3 mM과 0.55mmol/l/min로 관찰되었다. 화학변형 실험의 결과로부터 glutathione S-transferase $\rho$의 촉매가능과 관련된 아미노 group의 존재 가능성을 추정하였다. Glutathione S-transferase $\rho$ (EC 2.5.1.18) has been purified to homogeneity from human erythrocytes. A combination of gel filtration, ion exchange and hydroxylapatite chromatographic procedure yields the specific activity of 20.8 units/mg. The purified enzyme gives a single band corresponding to 24,000 M.W. on a sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme molecule is characterized tone an acidic protein (pI 4.6) having a dimeric structure with 48,000 M.W. composed of identical size of polypeptide chains. Apparent $K_m$ and $V_{max}$ were determined to be 1.1 mM and 1.0 mmol/l/min for 1-chloro-2, 4-dinitro benzene respectively while 0.3 mM and 0.55 mmol/l/min for glutathione. Results obtained from chemical modification studies suggest that essential amino group(s) critically connected to the catalytic function of glutathione S-transferase $\rho$.

러버쉬팅변환을 통한 「동궐도(東闕圖)」의 평면도 제작 가능성 연구 - 창덕궁 금천교 주변을 중심으로 -

이재용,김영모,Lee, Jae-Yong,Kim, Young-Mo 국립문화재연구원 2017 헤리티지:역사와 과학 Vol.50 No.4

The present study attempted to produce the floor plan of the surroundings near Geumcheongyo Bridge in Changdeokgung Palace of the Late Joseon Period through the use of rubber sheeting transformation based on the drawing principles of "Donggwoldo(東闕圖)". First, the study compared the actual sizes of the major buildings that have existed since the production of "Donggwoldo(東闕圖)" with the sizes depicted in the picture to reveal that the front elevation of the buildings was produced by reducing it by approximately 1/200. However, the study could not confirm the same production proportions for the side elevation. Only the lengths of the side elevation were depicted at around half of the actual proportions, and as the diagonal line angles were found to be at an average of $39^{\circ}$, the study confirmed they were drawn in a manner similar to cabinet projection. Second, the study created an obliquely projected floor plan by inversely shadowing the drawing principles of "Donggwoldo(東闕圖)" and produced a floor plan of the surroundings near Geumcheongyo Bridge in Changdeokgung Palace through the use of rubber sheeting transformation. Projective transformation was confirmed as most suitable during the transformation, and with standard error of 2.1208m, the relatively high accuracy of the transformation shows that the production of a floor plan for "Donggwoldo(東闕圖)" is significant. Furthermore, it implies the possibility of producing floor plans for various documentary paintings produced using the paralleled oblique drawing method in addition to "Donggwoldo(東闕圖)". Third, the study evaluated the accuracy of the spatial information provided by the produced floor plan by comparing the three items of Geumcheongyo Bridge location, Geumcheongyo Bridge and Jinseonmun Gate arrangement, and Geumcheon stone embankment location. The results confirmed the possibility of utilizing the floor plan as a useful tool which helps understand the appearance of the surroundings at the time of "Donggwoldo(東闕圖)" production because it is parallel to the excavation results of the Geumcheongyo Bridge and its context. Therefore, the present study is significant in that it seeks the possibility of producing spatial information recorded in "Donggwoldo(東闕圖)" by applying rubber sheeting transformation and consequently in that it presents a new methodology for understanding the appearance of the East Palace of the Late Joseon Period. 본고는 "동궐도(東闕圖)"의 작도원리를 기반으로 러버쉬팅변환(Rubber Sheeting Transformation)을 통해 조선후기 창덕궁 금천교 주변의 평면도 제작을 시도하였으며, 연구결과는 다음과 같다. 첫째, "동궐도(東闕圖)" 제작 당시부터 현존하는 주요 전각의 실제 크기와 그림에 묘사된 크기를 비교하여 전각의 정입면이 약 1/200으로 축소되어 작성되었음을 밝혀냈다. 그러나 측입면에서는 동일한 제작 비율이 확인되지 않았다. 다만, 측입면의 길이가 실제의 약 절반 정도로 그려지고, 사선 각도가 평균 $39^{\circ}$로 파악됨에 따라 캐비닛 투영(Cabinet Projection)과 유사한 방식으로 작도되었음을 확인하였다. 둘째, "동궐도(東闕圖)"의 작도 원리를 역추적하여 "사투영평면도"를 작성하고 러버쉬팅변환을 통해 "동궐도(東闕圖)"의 창덕궁금천교 주변 평면도를 제작하였다. 변환 시 투영변환(projective transformation)이 가장 적합한 것으로 확인되었고, 표준 오차는 2.1208m로 비교적 높은 정확도를 나타냄으로써 "동궐도(東闕圖)"의 평면도 제작이 유의미하였다. 이는 "동궐도(東闕圖)"뿐만 아니라 평행사선도법으로 작성된 각종 기록화의 평면도 제작 가능성을 시사한다. 셋째, 작성된 평면도가 제공하는 공간 정보의 정확성을 검토하기 위해 금천교의 위치, 금천교와 진선문의 배치, 금천의 석축 위치를 시기에 따라 비교하였다. 그 결과 작성된 평면도의 내용이 금천교 발굴조사결과와 그 맥락을 함께 함으로써 러버쉬팅변환이 "동궐도(東闕圖)" 제작 당시 모습을 이해하는데 유용한 도구로서의 활용 가능성이 검증되었다. 본 연구는 러버쉬팅변환을 적용하여 "동궐도(東闕圖)"에 수록된 공간정보를 2차원 평면도로 제작하기 위한 가능성을 모색하고, 이를 통해 조선후기 동궐의 모습을 이해하기 위한 새로운 방법론을 제시하였다는데 의의가 있다.

U-City의 성공적 구현을 위한 테스트베드 기본계획의 기준 수립에 관한 연구

이재용,피민희,정주영,신동빈,Lee, Jae-Yong,Pi, Min-Hee,Jung, Ju-Young,Shin, Dong-Bin 한국공간정보학회 2010 한국공간정보학회지 Vol.18 No.1

최근 많은 R&D 사업에서는 연구개발 완료 후, 연구 성과물들을 검증하기 위한 테스트베드사업이 다양하게 추진되고 있으나 일관적이고 체계성 있는 기준이 설정되어 있지 않은 실정이다. 따라서 본 연구에서는 체계적인 U-City 테스트베드 구축을 위해 기본계획의 기준을 도출하고, 도출된 기준에 근거하여 청라지역을 사례로 U-Eco City 테스트베드 기본계획(안)을 수립하여 검증하고자 한다. 이와 같이 설정된 U-City 테스트베드 기본계획 기준을 통해 테스트베드 특성에 맞는 체계화되고 합리적인 테스트베드 구축이 진행될 수 있을 것이다. Even after many constructions of test-beds to verify R&D results, there is no standard to set up a consistent and systematic test-bed master plan. Therefore, this research derives standards of a u-city test-bed master plan. To verify these standards, the research constructs the potential U-City master plan targeting Cheongna district in Incheon. Standards of a test-bed master plan which is suggested by this research will bring strengths to construct a test-bed such as clear construction procedures from presenting directions of planning, saving cost, effective management of equipments, and so on.

20세기 미술사조와 현대일러스트레이션에 관한 연구

이재용(Lee Jae young) 한국일러스아트학회 1998 일러스트레이션학 연구 Vol.3 No.-

Illustration has permeated people`s lives deep into their skin from the bottom of the pop culture. It has created its own values enough to concoct the biased view which considers it a simple method for commercial communications. Differently from the pure art which put stress on art spirit isolated from the general people, illustration has not only presented the possibilities of shortening the distance between the pure art and the general people but also established its own position in art as a new genre. In order to analyze the basis of the general aspects of modern illustration, this study tried to examine how the illustration was affected by the artistic understanding and expression methods of the 20th century painting and to grasp the modern concepts and formation ideology of the illustration.

산업디자인의 학문적 특성과 역할에 관한 연구 : 이론과 실천 사이의 과정으로써의 학문적 특성을 중심으로

이재용(Lee Jae Young) 한국디지털디자인학회 2004 디지털디자인학연구 Vol.4 No.1

리더십의 유형에 따른 근로자의 직무만족이 결과행위에 미치는 영향에 관한 연구

이재용(Lee, Jae-Yong),조정화(Cho, Jeong-Hwa),김영재(Kim, Young-Jae) 동아대학교 경영문제연구소 2013 經營論叢 Vol.34 No.-

The purpose of this study is to examine the effects of workers" job satisfaction on the outcome behaviors like organizational committment, organizational citizenship behavior by using managers" leadership style as the moderating variable. For this purpose, this study conducted a questionnaire survey of 428 workers from businesses located in Gyeongsang province. Data from the survey were statistically analyzed using One-Way ANOVA and ANCOVA. Findings of the study can be summarized as follows. Working condition improvement is required to make real the effects of superiors" transactional leadership on improving the subordinates" organizational committment, organizational citizenship behavior. This indicates that business organizations need to pay a great attention to upgrade their working conditions. Good relationships between the superiors and the subordinates are the key to making the former"s transformational leadership really effective. This suggests that business organization should promote so-called job-enrichment, for example, diversifying tasks given to their workers and allowing them to perform such tasks in a more autonomous way.

탁수에 의한 금강모치(Kumgang fat minnow; Rhynchocypris kumgangensis) 개체군의 생태적 영향1a

이재용 ( Jae Yong Lee ),최재석 ( Jae Seok Choi ),김재구 ( Jai Ku Kim ),장영수 ( Young Su Jang ),이광열 ( Kwang Yeol Lee ),김범철 ( Bom Chul Kim ) 한국환경생태학회 2008 한국환경생태학회지 Vol.22 No.2

어류개체군에 대한 탁수의 영향을 파악하고자, 2004년부터 2005년까지 탁수하천(대기, 자운천)과 비탁수하천(봉산천, 계방천)에 분포하는 금강모치 개체군의 체장-체중 관계와 비만도지수의 변화를 알아보았다. 탁수하천과 비탁수하천 간의 금강모치 평균전장을 비교해본 결과, 유의한 차이가 없는 것으로 나타났다. 남한강에서 체장-체중 관계에 따른 회귀계수 b값은 봉산천에서 3.21, 그리고 대기천에서 3.07로 나타났다. 이에 반하여, 북한강에 위치한 탁수하천의 회귀계수 b값은 비탁수하천의 값보다 약간 높았다. 그 값은 계방천에서 3.20, 자운천에서 3.23으로 각각 나타났다. 우리의 연구 결과는 금강모치 개체군 단계에서 만성 탁수의 영향이 적음을 보여준다. To investigate the effect of turbid water on fish population, the length-weight relationship and its condition factor were applied to Kumgang fat minnow Rhynchocypris kumgangensis collected in turbid (the Daegi and the Jaun streams) and non-turbid stream (the Bongsan and the Gyebang streams) for a year from 2004 to 2005. The mean length of minnow between turbid water and non-turbid water streams was not significantly different. In the South River system, the regression coefficients (b) based on length-weight relationship were 3.21 in the non-turbid Bongsan stream and 3.07 in the turbid Daegi stream, respectively. On contrary, the regression slope in the turbid stream was a little higher than that of the non-turbid stream located in the Bukhan River system. The values were 3.20 in the Gyebang-stream and 3.23 in the Jaun stream, respectively. Our result suggested that chronic turbid water effect on the level of fish population has a little or no effect.

부항 장치용 압력 제어 밸브 개발

이재용 ( Jae Yong Lee ),심동욱 ( Dong Wook Shim ),안수광 ( Soo Kwang An ),김은석 ( Eun Seok Kim ),이병렬 ( Byung Ryul Lee ),양기영 ( Gi Young Yang ) 경락경혈학회 2021 Korean Journal of Acupuncture Vol.38 No.4

Objectives : This study aimed to develop a cupping pressure control valve for limiting maximum negative pressure while achieving clinical therapeutic outcomes to minimize side effects induced by excessive negative pressure of air extraction cupping devices. Methods : To determine the clinical necessity and suitability of the cupping pressure control valve, this study was designed to measure the change in pressure with or without the valve using both a manual and an electric suction pump. Results : While the maximum pressure was limited by the pressure control valve, the pressure did not increase above a certain level regardless of the type of manual or electric pump. Conclusions : This study will contribute to the development of a safer and more effective base technology for cupping treatment in oriental medicine.

공동주택 관리소장의 서번트 리더십이 입주자만족도와 관리업체 재계약율에 미치는 영향

이재용(Lee, Jae-Young),이도화(Lee, Do-Hwa),최서현(Choi, Seo-Hyun) 한국주거학회 2016 한국주거학회 논문집 Vol.27 No.5

This study aims to verify the effects of the apartment office managers’ servant leadership on the residents’ satisfactions and housing management company contract renewal ratio, and to examine whether the number of apartment units and the apartments’ average size have the moderating effects upon the relationship between servant leadership and the residents’s satisfaction and housing management company contract renewal ratio. For the purpose of testing the hypotheses, the questionnaire survey was conducted targeting 1,000 apartment office staffs and 8,000 apartment residents of 200 apartment complex in the region of Ulsan, Kyungnam, and Busan, and the statistical analyses were carried out for the useful questionnaires of 961 apartment office staffs and 7,119 apartment residents. The main results are as follows: First, the higher the office managers servant leadership is, the higher the residents’ satisfactions. Second, the number of apartment units and the average size have moderating effects on the relationship between the office managers’ servant leadership and housing management company contract renewal ratio. Finally, the summary, implications, and limitations of this study, and the future research direction were discussed in the conclusion.