韓國國會의 機能分析에 관한 硏究

李長夏 서울市立大學 1985 국내석사

백서의 두정골 결손부 모델에서 maltodextrin-thrombin collagen이 골 재생에 미치는 효과의 평가

이장하 강릉원주대학교 일반대학원 2020 국내박사

백서 두정골의 결손부에 maltodextrin-thrombin (MT) collagen에 적신 콜라겐 플러그를 삽입한 그룹, 결손부에 콜라겐 플러그만을 삽입한 그룹, 결손부를 그대로 둔 그룹 간에 신생골 형성의 정도를 비교, 분석하여 MT collagen이 신생골의 형성에 미치는 영향을 알아보고자 한다. 이를 위해 36마리의 백서를 무작위로 12마리 씩 3그룹으로 나눈 후, 두개골의 정중봉합부에 직경 8㎜ trephine bur로 한 개의 골창을 형성한 후, 각각의 골 결손부를 다음과 같이 처치한다. Group 1은 결손부에 아무런 처치를 하지 않고 다시 봉합한다. Group 2는 골 결손부에 콜라겐 플러그만을 삽입하고, Group 3은 LMT collagen을 적신 콜라겐 플러그를 삽입한 후 다시 봉합한다. 4주, 8주 후에 각 그룹마다 6마리씩 희생시킨 후, 두개골 부위 표본을 채취하여 micro-computerized tomography(μ-CT)와 HE staining과 MT staining, 그리고 RUNX-2 및 osteoprotegerin에 대한 immunohistochemical(IHC) 분석을 시행한다. 결과적으로, LMT collagen을 적용한 collagen plug를 골 결손부에 적용하였을 경우, 아무 처치를 하지 않은 경우나 collagen plug만을 적용한 경우에 비해 신생골의 골 형성이 4주에서는 통계적으로 유의한 차이가 없었지만, 8주에서 통계적으로 유의하게 증가한 것을 알 수 있었다. 따라서 LMT collagen과 scaffold로 기능할 collagen plug의 조합이 골 결손부의 수복에 있어서 다양한 가능성과 선택지를 임상가에게 제공할 수 있을 것으로 기대된다. 주제어 : Maltodextrin-thrombin (MT) collagen, Fibrin, Lidocaine, Scaffold, Collagen plug, Bone regeneration, Rat calvarial model 이 성과는 2018년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(No.2018R1C1B5086579). (2020) We compared and analyzed the new bone formation between the group in which the collagen plug soaked in maltodextrin-thrombin (MT) collagen was inserted in the defect of the calvarial bone in the rat, the group in which only the collagen plug was inserted in the defect, and the group that left the defect intact, and investigate the effect of MT collagen on the new bone formation. To this end, 36 rats were randomly divided into 3 groups of 12, and a single bone window was formed with a trephine bur with a diameter of 8 mm on the median suture of the calvarial bone, and each bone defect was treated as follows. Group 1 is sutured again without any treatment on the defect. In Group 2, insert only the collagen plug into the bone defect, and in Group 3, insert the collagen plug soaked with LMT collagen, and then suture. After 4 weeks and 8 weeks, 6 animals were sacrificed in each group, and then samples of the skull area were collected and subjected to micro-computerized tomography (μ-CT), HE staining, MT staining, and immunohistochemical (IHC) analysis for primary antibodies of RUNX-2 and osteoprotegerin. As a result, there was no statistically significant difference in bone formation at 4 weeks when the collagen plug to which LMT collagen was applied was applied to the bone defect, compared to the case where no treatment was applied or only the collagen plug was applied. However, it was found that it increased statistically significantly at 8 weeks. Therefore, it is expected that the combination of LMT collagen and a collagen plug that will function as a scaffold can provide clinicians with various possibilities and options for repairing bone defects. Key words : Maltodextrin-thrombin (MT) collagen, Fibrin, Lidocaine, Scaffold, Collagen plug, Bone regeneration, Rat calvarial model This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIT) (No.2018R1C1B5086579).

맨드라미(Celosia cristata L.) 汁液의 담배 모자이크 바이러스에 대한 感染抑制 效果

이장하 강원대학교 대학원 1996 국내석사

Inhibitory effects of tobacco mosaic virus (TMV) infection were conducted on Nicotiana tabacum cv. Xanthi nc using the crude sap extracted from leaf, flower, stem and root of Celosia cristata L. As a result, crude sap extracted from leaf and root showed inhibitory effect as 100%. When TMV was inoculated on the half-leaf of Xanthi nc plant after coating the crude sap, inhibitory effect was similar to the result of TMV inoculation mixed with the crude sap. However, inhibitory effect of TMV infection before coating the sap was not found, In 80% TMV inhibition of the sap, thermal inactivation point was 80℃ and the inhibitory effect retained at the dilution of 1/500. Longevity in vitro of the sap was maintained even after 30 days. The crude sap from C. cristata was also inhibited the turnip mosaic virus and cucumber mosaic virus with high levels.

국내 노지재배 고추의 바이러스 발병현황과 ChiVMV의 저항성 분자표지 개발

이장하 강원대학교 2013 국내박사

Viral Diseases of Pepper in Field Cultivation in Korea and Development of Resistant Molecular Markers for Chilli veinal mottle virus in Capsicum annuum L. Jang Ha, Lee Department of Applied Biology Graduate School, Kangwon National University Abstract In this study, viral disease samples were obtained in pepper fields in 11 major pepper-growing districts in Gangwon-do and in 83 areas from other provinces except Gyeongsnagnam-do and Jeju island in Korea during the 2006 and 2010. In order to assess their infection types, the field surveys had been conducted based on viral disease severity, virus type, and virus infection type of typical symptoms on leaves. A means of single and mixed viruses-infection were 46.6% and 48.0% from those periods, respectively, suggesting that viruses are most severely influence agent to decrease pepper production in field cultivation in Korea. In a single infection, Cucumber mosaic virus (CMV) was defined the most prevalent virus on the basis of its disease severity ratings, through 55.3% and 77.4%. Then, Board bean wilt virus 2 (BBWV 2), Pepper mild mottle virus (PMMoV), and Pepper mottle virus (PepMoV) were shown to be caused by altering severe viral disease agents in pepper around 5 to 10% disease severity. Whereas, Tomato spotted wilt virus (TSWV) has been occurred in a limited areas involving Chungcheongnam-do and Jeollanam-do, and in case of Tobacco mild green mottle virus (TMGMV) has not been detected in tested samples in present study, even though previous reports had been shown its occurrence. Thus, viral disease severity in pepper can be severe by CMV, BBWV 2, PMMoV, and PepMoV, and those virus types should be continued to be critical for decreasing pepper production in field cultivation in Korea. Additionally, mixed-infections were frequently observed beside single infection from collected pepper samples from all areas. The ratios of mixed-infection, therefore, were studied to evaluate the level of mixed-infections disease severity and the definition of virus type individually. These data showed that different types of viruses were presented and CMV was the most abundant virus for mixed-infection just as in case of single infection. Among them, the highest mixed-infection disease severity was CMV + BBWV and then the types of mixed-infection such as CMV + PepMoV and CMV + PMMoV were followed on the basis of mixed-infection severity. However, further work is needed to assess more than double mixed-infection types such as triple or more. A biological control study using avirulent CMV KPV14 in 2008 and 2009 was performed. Four cultivars (PR Manidda, Supermanidda, Daecheon, PR Daecheon) in treated and non-treated avirulent CMV KPV14 were used in this study. The ratio of infection in virulent CMV was 28.3% in Daecheon, 19.4% in PR Manidda, 12.5% in Supermanidda, 7.1% in PR Daecheon higher than non-treated cultivar was. The ratio of infection in BBWV 2 was 38.2~55.0% in non-treated cultivar, and 41.9~53.1% in treated cultivar. These results was no significant between them. Furthermore, avirulent CMV KPV14 increased BBWV 2 occurrence in treated cultivar. Chilli veinal mottle virus (ChiVMV) is one of the most destructive pepper pathogens in Asia. Development of a ChiVMV-resistant cultivar is necessary to control ChiVMV infection on pepper farms. However, the genetic source of ChiVMV resistance has not been previously identified only the recessive resistance gene has been recognized. In order to further examine hereditary ChiVMV resistance and to establish a relevant breeding program, we firstly screened 30 pepper lines from several countries and, through inoculation tests, discovered a new resistant line. Here, we report a new genetically dominant resistant chilli pepper. Secondly, we found two amplified fragment length polymorphisms linked to dominant resistance and converted them into high-resolution melting markers, which were located on chromosome 6 of the pepper. Furthermore, we obtained a cleaved amplified polymorphic sequence marker closer to the ChiVMV resistance locus using comparative mapping. The newly discovered marker, related to a single dominant gene, will help researchers to develop a new ChiVMV-resistant pepper cultivar.

Retrospective study on alveolar bone grafting in cleft patients

이장하 강릉원주대학교 일반대학원 2016 국내석사

Purpose: In this retrospective study, we evaluated and compared the outcome depending on the type of different ossification process in patients with alveolar cleft that was performed the endochondral bone (iliac bone or rib bone) grafting or intramembranous bone (mandibular bone) grafting, and want to find more favorable conditions for successful bone grafting. Materials and methods: Medical records and radiologic images of patients who were performed the alveolar bone grafting due to alveolar cleft were analyzed retrospectively. Through postoperative and follow-up radiologic images of patients, height of interdental bone septum was classified into four types based on the highest point of alveolar ridge. Then the height of interdental bone septum and area of graft bone were evaluated according to the type of the graft bone. In addition, the occurrence of complications and additional bone graft, result of postoperative orthodontic treatment, and eruption of impacted tooth were investigated. Results: Finally, 33 patients were included in this study. There was no significance difference in the change of interdental bone height and area of graft bone according to the type of bone. There was no statistically significant difference in the success rate of surgery according to the type of bone. One patient received an additional bone graft surgery during follow-up period. There were no serious complications after surgery in all patients. Conclusion: Both intramembranous bone and endochondral bone can be used for bone grafting of alveolar cleft. The result is a successful, and there was no significant difference in the results between two groups. It is important to select the appropriate bone in accordance with the size and shape of the alveolar cleft site and condition of the patient. 목적: 이 연구에서는 연골성골 (장골과 늑골)이나 골막성골 (하악골) 이식술을 시술 받은 치조열 환자에서 서로 다른 골화 과정을 거친 이식골의 종류에 따른 결과를 비교, 분석하고, 성공적인 골 이식술을 위한 더 좋은 조건을 찾아보고자 하였다. 재료 및 방법: 치조열로 치조골 이식술을 시술 받았던 환자들의 의무기록지와 방사선 사진이 후향적으로 분석되었다. 수술 직후와 추적검사 기간 동안 촬영한 환자의 방사선 사진을 통해 치간골 높이를 치조정의 최상방점을 기준으로 4가지로 분류하였다. 수술의 결과와 치간골 높이의 변화를 이식한 골의 종류에 따라 평가하였다. 추가적으로 합병증의 발생이나 추가적인 골이식 여부, 수술 후 교정치료 결과, 매복치아의 맹출 여부 등을 조사하였다. 결과: 최종적으로 33명의 환자들이 이 연구에 선택되었다. 이식한 골의 종류에 따른 치간골 높이의 변화에는 유의할만한 차이가 없었다. 이식골의 종류에 따른 술식의 성공률에도 통계적으로 유의한 차이는 없었다. 한 명의 환자는 추적검사기간 동안 추가적인 치조골 이식술을 시술 받았지만, 모든 환자에서 수술 후 심각한 합병증은 관찰되지 않았다. 결론: 골막성골과 연골성골 모두 치조골 이식술에 사용될 수 있고, 그 결과는 성공적이다. 치조열의 크기가 형태, 화자의 상태에 따라 적절한 골을 선택하는 것이 더 중요하다.

러시아 아방가르드의 類型別 造形世界와 理念에 關한 硏究 : 絶對主義,構成主義,生産主義를 中心으로

이장하 弘益大學校 1987 국내석사

접착제법으로 제조된 알루미늄 허니콤 판재의 기계적 특성평가

이장하 서울대학교 대학원 1994 국내석사

學校敎育目標設定과 具現에 關한 實態分析硏究

이장하 建大行政大學院 1968 국내박사

Teratogenic Effects of 2-Naphthol and 2-Hydroxy Fluorene on Xenopus laevis development.

이장하 경북대학교 대학원 2023 국내석사

Poly Aromatic Hydrocarbons (PAHs)는 자동차 배기가스, 공장 매연, 흡연, 그을린 음식과 같은 인위적인 발생과 산불, 화산폭발과 같은 환경적인 발생을 통해 유기화합물이 불완전 연소되면서 발생하여 토양에 흡수되거나 대기중으로 분출되어 인체에 접촉한다. 과도한 노출로 인해 PAHs가 체내에 축적되면 폐 암, 췌장암, 전립선암 등을 유발한다고 알려져 있다. 본 연구에서 사용한 2- Naphthol과 2-Hydroxy Fluorene은 PAHs의 한 종류이며 5기 국민환경보건 기초조사에 따르면 어린이, 청소년, 성인의 소변에서 검출되는 환경유해인자이 다. 본 연구에서는 FETAX 실험을 통해 두 물질이 Xenopus laevis 배아에 노 출되었을 때 어떠한 발생학적 영향을 미치는지 확인해 보았다. 우선 2-Na에 노출되었을 때 배아는 length reduction, edema, tail flexure, small eye와 같은 기형들이 관찰되었고 2-HF에 노출되었을 때는 edema, tail flexure, pigment loss, misfolded gut, lens expansion과 같은 기형들이 관찰되었다. 여러 기형들 중에 본 연구에서는 눈과 관련된 기형에 집중하기로 하였다. 눈 전체적으로 발 현하는 six3, 망막 특이적으로 발현하는 rx1, 수정체 특이적으로 발현하는 cryba1, prox1을 마커로 사용하여 Whole-mount In Situ Hybridization (WISH), qRT-PCR 실험을 통해 눈 관련 마커들의 mRNA 공간적 발현 패턴과 총 발현 양을 알아보았다. 2-Na에 노출된 배아에서는 대조군 배아에 비해 six3, rx1의 발현양은 줄어들고 cryba1, prox1의 발현양은 변화가 없는 것으로 나타났다. 즉 수정체를 제외한 눈 크기가 줄어든 것을 확인하였다. 2-HF에 노출된 배아에 서는 대조군 배아에 비해 cryba1, prox1의 발현양은 증가하고 six3, rx1의 발현양은 줄어들었다. 즉 수정체의 크기는 늘었지만 눈 전체적인 크기는 변화가 없음을 확인하였다. 두 물질의 노출이 어떠한 방법을 통해 기형을 유발하는지 확인하기 위해 세포 발달에 중요한 두가지 요인인 세포이동의 변화와 Reactive Oxygen Species (ROS)의 축적을 β-Gal Staining과 HyPer 실험을 통해 확인해 보았다. 그 결과 2-Na과 2-HF에 노출된 배아 모두에서 대조군 배아에 비해 세포의 이동이 중앙선 부근으로 제한되는 것을 확인하였고 ROS 또한 대조군 배아에 비해 많이 축적되는 것을 확인하였다. 결론적으로 본 연구의 실험 결과 에 따르면 Xenopus 배아 발달 과정에서 2-Na와 2-HF에 노출되면 눈 관련 mRNA의 공간적 발현과 발현양을 조절하고 분화되는 세포의 이동을 제한하며 ROS의 축적을 유발하여 눈 기형을 유발함을 암시한다.