http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Mistletoe lectin I/D-galactose의 인식결합에 기초한 Mistletoe lecti I에 대한 용액상 효소결합분석법에 관한 연구
이인숙,이은아,전종순,Rhee Paeng, Insook,Lee, Eun Ah,Chon, Chong Soon 한국분석과학회 2000 분석과학 Vol.13 No.5
Mistletoe lectin I(ML I)에 대한 간단하고 빠른 용액상 효소결합 분석법을 렉틴의 당 특이성을 이용하여 개발하였다. ML I에 특이성을 가지고 있는 D-galactose를 사용하였으며, 용액상 분석법의 효소로는 malate dehydrogenase(MDH)를 사용하였다. 분석신호물질로 사용되는 MDH-galactose 접합체는 isothiocyanate 방법을 통해 합성하였으며, 이 접합제는 thiourea 결합을 하고 있다. ML I의 존재하에, ML I은 D-galactole와의 특이 인식결합을 통해 MDH-galartose 접합체의 활동도를 억제한다. 그러므로, 존재하는 ML I의 농도는 MDH-galactose 접합제의 촉매활동도의 억제도에 비례하게 된다. 따라서, 본 용액상 효소결합 분석법을 통하여 ${\mu}g/mL$ 수준의 ML I의 측정이 분석 시간 10분 이내에 가능하였다. A simple and rapid homogeneous enzyme-linked binding assay for mistletoe lectin I(ML I) was developed using a coupled enzyme system of malate dehydrogenase (MDH) and D-galactose. A highly substituted MDH-galactose conjugate was prepared by employing an isothiocyanate method for formation of thiourea bond. In the presence of ML I, ML I inhibits the activity of the conjugate based on the ML I/D-galactose specific interaction. Thus, the concentration of ML I can be related to the homogeneous inhibition of the MDH-galactose conjugate. Using this method. ML I can be measured at the level of microgram per milliliter within 10 minutes.
이인숙,김은영,이보경,팽기정,Rhee, Insook,Kim, Eun Yong,Lee, Bokyoung,Paeng, Ki-Jung The Korean Electrochemical Society 2015 한국전기화학회지 Vol.18 No.2
A method for degradation of the perchlorate anion ($ClO{_4}^-$) has been studied using electrochemically generated zero-valent iron (ZVI) deposited on a porous carbon electrode. The first strategy of this study is to produce the ZVI via the electrochemical reduction of iron (II) on a porous carbon electrode coated with a conducting polymer, instead of employing expensive $NaBH_4$. The present method produced well distributed ZVI on conducting polymer (polypyrrole thin film) and increased surface area. ZVI surface can be regenerated easily for successive reduction. The second strategy is to apply a mild reducing condition (-0.3 V) to enhance the efficiency of the degradation of perchlorate with ZVI without the evolution of hydrogen. The electrochemically generated ZVI nanoparticles may offer an alternative means for the complete destruction perchlorate without evolution of hydrogen in water with high efficiency and at low cost.
고분자결합 금속포르피린을 촉매로 한 2, 4, 6-트리클로로페놀의 산화 분해반응
박혜옥,이보영,이인숙,Park, Hye-Ok,Lee, Bo-Young,Rhee Paeng, Insook 한국분석과학회 2001 분석과학 Vol.14 No.1
금속포르피린과 그들의 고분자결합 유도체를 산화촉매로 한 2, 4, 6-trichlorophenol(TCP)의 산화분해반응을 수행하였다. 산화제로는 $KHSO_5$와 tert-butylhydroperoxide(TBHP)가 사용되었다. 금속포르피린은 TCP의 산화분해반응에서 매우 낮은 촉매활성을 보인 반면, 금속포르피린이 새로이 합성된 고분자나 XAD2 수지에 화학결합 되어있는 고분자결합 금속포르피린을 촉매로 사용한 TCP의 산화 분해반응에서는 매우 높은 촉매활성을 보여주었다. 또한, 피리딘이나 이미다졸과 같은 전자 주개 리간드가 축방향에 놓여 있는 고분자의 경우에는 더 큰 활성을 나타내었다. Maleic acid와 chloromaleic acid는 생성물로서 ESI-MS로 확인되었다. 특히, XAD2 수지에 결합된 촉매의 경우 산화제에 대한 안정성과 용매에 대한 불용성으로 인해 촉매로서 재사용이 가능함을 확인하였다. Oxidative decomposition of 2, 4, 6-trichlorophenol(TCP) was studied in aqueous solution. Iron and manganese protoporphyrin [or tetrakis(p-carboxyphenylporphyrin)] and their polymer supported derivatives were used as catalysts, and $KHSO_5$ and tert-butyldroperoxide(TBHP) as oxidants. Metalloporphyrin itself shows very poor catalytic activity in oxidative decomposition of TCP with oxidant. However, very high catalytic activity was observed when metalloporphyrin was chemically bound to newly synthesized polymers or XAD2 resin. Additionally, it revealed much higher catalytic activity in the presence of water-soluble polymers having a electron-donating axial ligand such as pyridine and immidazole. Maleic acid and chloromaleic acid were found in the resulting solution by ESI-MS. Especially, XAD2-supported metalloporphyrins can be reused as catalysts due to insolubility to solvent, and stability against oxidant.
수은 막전극을 이용한 수용액 중 과염소산이온의 전기화학적 환원
명노승,김은영,지형우,금나래,이인숙,팽기정,Myung, Noseung,Kim, Eun Young,Jee, Hyung-Woo,Keum, Narae,Rhee, Insook,Paeng, Ki-Jung 한국전기화학회 2016 한국전기화학회지 Vol.19 No.3
A method for electrochemical degradation of the perchlorate anion ($ClO_4{^-}$) using mercury film electrode has been studied. Electrochemical method has relatively simple pre-treatment. However, electrochemical method should avoid interference from hydrogen evolution at the applied potential to degradation of perchlorate ion, and thus applied electrode should have large hydrogen overvoltage which suppressed the hydrogen evolution at the working reduction potential to prevent hydrogen evolution. In this study, we used mercury film electrode as a working electrode which has a large overvoltage. Ag / AgCl (sat. NaCl) was used as a reference electrode, and platinum was used as a counter electrode. Mercury film electrode was made by cyclic voltammetry (CV) method. The deposition time was decided as 10 minute, and the stability of the mercury electrode in perchlorate solution was confirmed by CV. The reduction potential of perchlorate was checked by using CV method, and decomposition of perchlorate was performed by using chronoamperometric (CA) method. Also, ion chromatography (IC) was used to confirm the degradation rates of perchlorate. 과염소산이온의 전기화학적 분해에 관한 연구가 진행되었다. 전기화학적 방법은 비교적 단순한 전처리 방법으로 가능하다. 하지만 전기화학적 방법은 과염소산이온이 분해되는 전압을 가해주었을 때 수소발생으로 인한 방해가 발생하기 때문에 사용하는 전극의 수소 과전압이 큰 것을 사용하여 수소발생을 줄이는 것이 요구되어 왔다. 본 연구에서는 수소 과전압이 큰 수은박막전극을 작업 전극으로 사용하였다. Ag / AgCl (sat. NaCl) 전극을 기준전극으로 사용하였으며 Pt를 상대전극으로 사용하였다. 수은박막전극은 순환 전압-전류법(Cyclic voltammetry, CV)으로 제작하였는데 과염소산 용액에서의 CV를 고려하여 수은박막전극의 안정성을 위해 10분 동안 전착시켰다. 과염소산이온의 환원전위는 CV 방법에 의해 설정되었고, 분해실험은 시간대 전류법 (Chronoamperometry, CA)로 시행하였다. 과염소산이온의 분해율 확인을 위해 이온 크로마토그래피(Ion chromatography, IC)를 사용하였다.
Fast mixing device를 이용한 Protein sample의 Time-Resolved Resonance Raman Spectroscopy
이인숙 서울여자대학교 자연과학연구소 2003 자연과학연구논문집 Vol.15 No.-
The application of fast mixing devices on the heme protein samples was described. Obviously, it is important to determine whether protein samples are stable under conditions encountered with new devices. Human Hemoglobin (Hb) solution was introduced into Platinum sphere mixing (PSM) system at 20 lb/in² gas pressure. The results were checked by UV-Vis spectroscopy and resonance Raman spectroscopy, and the spectra are superimposable upon those obtained from the initial Hb solution, respectively. The ligand binding reaction (oxygenation) of deoxy Hb was foIIowed by PSM. Time-resolved resonance Raman spectroscopy shows that the oxygenation reaction of Hb can be measured with PSM device for resonance Raman studies. Also, resonance Raman spectrum of product of the reaction between met Hb and HzOz supports that this Hb-H₂O₂ complex is ferryl Hb.