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팔레놉시스 생육에 적합한 배양액내 NO₃⁻와 NH₄⁺ 비율
이영란(Young Ran Lee),이용범(Yong Beom Lee),예병우(Byeong Woo Yae),이동수(Dong Soo Lee) (사)한국생물환경조절학회 2010 시설원예‧식물공장 Vol.19 No.4
본 연구는 팔레놉시스의 생육에 적합한 배양액내 NO₃?와 NH₄?의 비율을 찾고 그리고 그 비율이 화경의 품질에 미치는 영향에 대하여 알아보고자 수행되었다. 실험에 사용된 배양액내 NO₃?와 NH₄?의 비율은 100% : 0%, 90% : 10%, 80% : 20%, 70% : 30%였다. 배양액내 팔레놉시스의 생육은 NO₃?만을 공급할 때보다 NO₃?와 NH₄?를 함께 공급할 때 생육이 증가하였다. 특히 NH₄?의 비율이 10%일 때 생체중과 건물중이 가장 높았다. 그러나 배양액내 NH₄?의 비율이 10%에서 30%로 증가함에 따라 지상부와 뿌리의 생육은 감소되었다. 화경의 길이, 개체당 화경수와 소화수 모두 NH₄? 비율이 0%에서 10%로 증가함에 따라 높았으나 30%로 높아질수록 감소하였다. 즉, 팔레놉시스의 생육에 적합한 배양액내 NO₃?와 NH₄?의 비율은 90% : 10%이었다. This experiment was carried out to find optimum ratio of NO₃? to NH₄? in nutrient solution for the growth of Phalaenopsis hybrid and find the effect of the ratio of NO₃? to NH₄? on the flower stem (inflorescence) quality. The ratio of NO₃? to NH₄? of nutrient solution used in this experiment was 100% : 0%, 90% : 10%, 80% : 20% and 70% : 30%. Phalaenopsis showed better growth when NH₄? was supplied concurrently with NO₃? as nitrogen source than supplied with only NO₃?. Especially, increasing the ratio of NH₄? from 0% to 10% the fresh weight and dry weight of Phalaenopsis hybrid was highest. But, the growth of shoot and root was diminished when the proportion of NH₄? in nutrient solution was increased from 10% to 30%. Inflorescence length, the number of inflorescence and flower per plant all increased as NH₄? increased from 0% to 10% but, decreased from 10% to 30%. These results suggest that the optimal ratio of NO₃? and NH₄? in nutrient solution for the growth of Phalaenopsis including inflorescence was founded to be 90%:10%.
분무경 시스템에서 팔레놉시스 유묘 생육에 적합한 분무간격
이동수(Dong Soo Lee),권오근(Oh Keun Kwon),이용범(Yong Beom Lee),예병우(Byeong Woo Yae),이영란(Young Ran Lee) (사)한국생물환경조절학회 2010 생물환경조절학회지 Vol.19 No.4
분무경시스템에서 분무간격과 분무시간은 식물의 생육에 필요한 양분과 수분을 공급하기 위한 중요한 요소들이다. 이 실험은 분무경에서 분무간격이 팔레놉시스 유묘의 생육에 미치는 영향에 대하여 알아보고자 수행되었다. 배지 없이 뿌리가 나출된 생체중 16g 크기의 유묘를 사용하였다. 분무간격은 각각 10, 20, 30, 40, 50분 간격이었으며, 분무시간은 10분이었다. 총생체중, 총건물중, 분지된 뿌리수와 상대생장률은 20분과 30분 간격에서 10분, 40분, 50분 간격보다 높았다. 특히, 뿌리의 생체중은 30분 간격에서 가장 높았다. 엽장은 30분 간격에서 길었으나, 엽폭은 처리간 차이가 없었다. 10분 간격 처리에서 유묘한 개체가 30일간 소비한 물량은 0.71L였으며, 분무간격이 증가함에 따라 그 양은 감소하였다. 15일간 소비된 양분은 칼륨이외에는 처리간 차이가 없었으며, 칼륨은 30분 간격에서 가장 많이 흡수되었다. 결과를 요약하면, 분무경 시스템에서 팔레놉시스 유묘 생산을 위한 적정 분무간격은 30분이었다. The irrigation interval and time for the supply of nutrient and water to the plant is important in the aeroponic system. This study was conducted to investigate the effect of irrigation interval on the growth of Phalaenopsis hybrid seedlings in the aeroponic system. Young bare-root plants (four leaves, 16 g in fresh weight) were used for this experiment. The composition of nutrient solution was, in meㆍL?¹, 9 N, 3 P, 4 K, 4 Ca and 2 Mg. The electrical conductivity (EC) and pH of nutrient solution used was 1.2 dSㆍm?¹ and 5.8, respectively. Irrigation intervals were 10, 20, 30, 40, and 50 minute and each irrigation time was 10 minute. The total fresh and dry weight, the number of branched roots, and relative growth rate at the 20 and 30 min. was greater than 10, 40, 50 min. interval. Especially, the fresh weight of roots at 30 min. interval was the highest. Leaf length was the highest at 30 min. interval but there was no difference in leaf width. The amount of water consumed for a month was 0.71 L per plant and it was reduced with increasing irrigation interval. There was no difference in the amount of consumed mineral contents for 15 days except for potassium. Potassium absorbed was the highest at 30 min. irrigation interval. As a result, the optimum irrigation interval was 30 min for the production of Phalaenopsis hybrid seedlings in the aeroponic system.
매트재배에서 Ardisia 소형분화 생산에 적합한 배지의 물리성
이동수(Dong Soo Lee),권오근(Oh Keun Kwon),이영란(Young Ran Lee),이용범(Yong Beom Lee) (사)한국생물환경조절학회 2009 생물환경조절학회지 Vol.18 No.4
배지의 적정 수분함량과 기상율은 분화식물 생산에 있어 중요한 환경 요인들이다. 매트관수시스템에서 Ardisia 소형분화 생산에 적합한 배지의 물리성을 구명하고자 실험을 수행하였다. 실험에 사용된 공시 작물은 산호수와 자금우였다. 피트모스 배지에 왕겨와 펄라이트를 각각 부피비로 20, 40, 60%로 혼합하였다. 배지의 총공극은 왕겨의 혼합비율이 증가함에 따라 증가하였으나, 펄라이트는 혼합비율이 증가함에 따라 감소하였으며, 왕겨와 펄라이트의 혼합비율이 증가함에 따라 기상율은 증가하였으나, 배지의 수분함량은 감소하였다. 배지의 기상율은 왕겨 혼합배지에서 펄라이트 혼합배지보다 높았으며 증가율은 왕겨혼합배지에서 더 높았다. 배지의 CO₂ 농도는 기상율이 증가함에 따라 감소하였는데, 왕겨 혼합배지에서 펄라이트 혼합배지보다 CO₂ 농도가 더 높았다. 산호수와 자금우의 생체중과 건물중은 왕겨를 60% 혼합한 배지에서 가장 높았으나, 지상부와 지하부의 건물 비율은 가장 낮았다. 매트관수 시스템에서 Ardisia속 식물인 산호수와 자금우의 소형분화 생산에 적합한 배지의 물리성은 총공극과 기상율, 포트용수량이 각각 82.8, 25.6, 57.2%이었다. Adequate conditions of water content and aeration of container media are major environmental factors in the production of pot plant. This experiment was carried out to find optimum physical property of media for the production of small potted Ardisia in capillary mat irrigation system. The plant materials used in this experiment were Ardisia pusilla and Ardisia japonica. Seven substrates were formulated by blending perlite or fresh rice hulls at 20%, 40%, 60% (v/v) with sphagnum peat. Total pore space (TPS) increased by blending sphagnum peat with fresh rice hulls, but decreased by blending sphagnum peat with perlite. As fresh rice hull (FRH) and perlite content increased, air filled pore space (AFP) of substrate increased but container capacity (CC) decreased. Substrate blended with fresh rice hull was higher AFP than blended with perlite and the rate of increase was higher for FRH-containing substrate. As AFP increased, the CO₂ concentration in the pot decreased and the CO₂ concentration of substrate blended with FRH was higher than blended with perlite. The fresh and dry weight of Ardisia pusilla and A. japonica was the highest in the substrate contained 60% FRH, but the ratio of shoot dry weight to root dry weight was the lowest. The optimum total pore space, air-filled pore space, water holding capacity of substrate for the growth of Ardisia pusilla and A. japonica in the capillary mat irrigation system were 82.8%, 25.6%, and 57.2% respectively.
SRAP과 SSR 마커를 이용한 국내 육성 팔레놉시스 품종의 유전적 다양성 분석과 품종판별
박부희(Pue Hee Park),박용진(Yong-Jin Park),김미선(Mi Seon Kim),이영란(Young Ran Lee),박필만(Pil Man Park),이동수(Dong Soo Lee),예병우(Byeong Woo Yae) 한국원예학회 2013 원예과학기술지 Vol.31 No.3
본 연구의 목적은 SSR과 SRAP 마커 시스템을 이용하여 팔레놉시스 14품종 간 유전적 거리를 비교하고, SSR 마커를 이용하여 품종 간 구분을 하기 위한 것이다. 전체적으로 111개의 SSR 프라이머와 30조합의 SRAP primer를 먼저 스크리닝하였다. 국립원예특작과학원에서 보존중인 국내 육성품종을 포함한 14품종의 팔레놉시스에서 12개의 SSR 프라이머와 30조합의 SRAP 프라이머에서 높은 다형성을 보였다. 증폭된 DNA 단편들은 acrylamide gel에서 분리시킨 후 silver staining 방법으로 검출하였다. SSR 마커 55개와 SRAP 419개로, 총 474개의 마커를 획득하였으며 이를 유전적 다양성 분석에 사용하였다. 다형성 밴드들은 MVSP 3.1 프로그램을 이용하여 유전적 유사도와 UPGMA clustering 분석을 위해 scoring 되었다. 14 팔레놉시스 품종은 SRAP과 SSR 분석을 통해 각각 0.683과 0.66의 유사도 지수에서 3그룹으로 분류되었다. 또한 SSR 20번과 22번만으로도 이들 육성 품종을 구분할 수 있었다. 이 결과는, SSR 분석은 팔레놉시스 품종간 구분에 효과적이고 SRAP은 염기서열의 정보가 없을 때 유전적 다양성 분석에 유용하다는 것을 보여준다. 이번 연구된 SSR과 SRAP 마커들은 팔레놉시스의 유전자형 판별, 유전자원 보존, 유전적 근연관계를 분석하는데 유용한 기술이 될 것이다. The aims of this study were to compare genetic distances among 14 Phalaenopsis varieties using simple sequence repeat (SSR) and sequence-related amplified polymorphism (SRAP) marker systems and to determine the discrimination using SSR. A total of 111 SSR primers and 30 SRAP combinations were initially screened. Twelve SSR primers and thirty SRAP combinations showed high polymorphism among the 14 Phalaenopsis varieties including domestic breeding varieties, conserved in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected by silver staining method. A total of 474 polymorphic bands, including 55 by SSRs and 419 by SRAPs, were identified and used for genetic diversity analysis. Polymorphic bands were scored for calculating a simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. Fourteen Phalaenopsis varieties were classified into three major groups at similarity coefficient value of 0.683 and 0.66 using SRAP and SSR, respectively. Also we could discriminate these domestic breeding Palaenopsis varieties using only SSR 20 and SSR 22. The results indicate that SSR analysis is effective for discrimination among Phalaenopsis varieties and SRAP is useful for genetic diversity when there is no sequence information. These studied SSR and SRAP markers will be useful tools for genotype identification, germplasm conservation and genetic relationship study in Phalaenopsis.
한국 서해 상괭이 (Neophocaena asiaeorienfalis)의 먹이습성과 섭식량
박겸준 ( Kyum Joon Park ),안용락 ( Yong Rock An ),이영란 ( Young Ran Lee ),박지은 ( Ji Eun Park ),문대연 ( Dae Yeon Moon ),최석관 ( Seok Gwan Choi ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.1
We investigated the stomach contents of fin less porpoises collected in the Yellow Sea. Prey organisms in the stomachs of 109 finless porpoises were identified as 11 species of fish, 8 species of shrimp and 4 species of cephalopod. Index of Relative Importance analysis revealed that the porpoises fed mainly on the Japanese sand shrimp (Crangon affinis). The energy density of the prey was estimated to be 5.46 kJ. The daily energy requirement was estimated to be 18,051 kJ/day for a porpoise with an average weight of 32.49 kg. The estimated annual consumption by fin less porpoises in the Yellow Sea was 25,454 tons. The average commercial catch in the Yellow Sea by Korean fisheries was 135,913 tons from 2005 to 2009. Assuming that the abundance of finless porpoises is stable, porpoises ate approximately 18.7% of the commercial catch. The fishery catch may be influenced by the consumption by fin less porpoises, while fishery bycatch is a cause of porpoise mortality in the Yellow Sea.
목시조사 (2000-2010)에 의한 한국 연안 고래류의 종류 및 분포
손호선 ( Haw Sun Sohn ),박겸준 ( Kyum Joon Park ),안용락 ( Yong Rock An ),최석관 ( Seok Gwan Choi ),김장근 ( Zang Geun Kim ),김현우 ( Hyun Woo Kim ),안두해 ( Du Hae An ),이영란 ( Young Ran Lee ),박태건 ( Tae Geon Park ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.5
in the late 1970s, the National Fisheries Research & Development institute (NFRDI) started cetacean research to submit the Korean whale catch record to the International Whaling Commission. This continued until the moratorium on commercial whaling in 1986. The NFRDI resumed Cetacean research with a pilot whale sighting survey in 1999. Subsequently, the NFRDI has conducted 53 cetacean sighting surveys within the Korean exclusive economic zone between 2000 and 2010. The surveys took a total of 760 days and cruising for 23.866 nautical miles. The finless porpoise Neophocaena asiaeorientalis was sighted most frequently (735 times), followed by the minke whale Balaenoptera acu-torostrata (396 times), the long-beaked common dolphin Delphinus capensis (102 times), and the Pacific white-sided dolphin Lagenorhynchus obliquidens (27 times). Minke whales were disiributed in the Yellow Sea and coastal area of the East Sea from spring to fall. Pacific white-sided dolphin sightings were restricted to the middle and upper coastal areas of the East Sea in summer. Common dolphins were sighted from east of the southern coast to the eastern coast of the Korean Peninsula from spring to fall. Finless porpoise occurred in all Korean coastal areas, except the middle and upper eastern coast.