단세포전기영동법(Single Cell Gel Electrophoresis Assay)을 이용한 농약 살포자의 DNA손상 평가

이연경(Yeon Kyeng Lee),이도영(Do Young Lee),이은일(Eunil Lee),이동배(Dong Bae Lee),류재천(Jae Chun Ryu),김해준(Hae-Joon Kim),설동근(Donggeun Sul) 한국환경성돌연변이발암원학회 2001 한국환경성돌연변이·발암원학회지 Vol.21 No.2

Single cell gel electrophoresis (SCGE) assay, also called comet assay, is a rapid and sensitive method to detect DNA damage in single cell level. To evaluate the DNA damage of lymphocytes of pesticides sprayers, SCGE assay was carried out for 50 pesticides sprayers and 58 control subjects. They were interviewed with structured questionnaire to get the information about the kinds and amount of pesticide. Insecticides and fungicides were predominant among pesticides. Major components of pesticides were organophosphorus, organosulfate, cartap, carbamates, and triazole. Sprayed pesticides were classified into two groups. Group I included organophosphorus, organoarsenic, organotin, tetrazine, triazole and gramoxone, which were known to cause DNA damages. Group II pesticide were carbamates, surfactants, organosulfates, etc., which were not found as DNA damaging agents in scientific documents. Olive tail moments of 100 lymphocytes were measured by<br/> KOMET 3.1 program for each person. The means of tail moments were compared between farmers exposed to pesticides and control subjects. Farmers showed higher tail moments than control subjects (2.07±1.40 vs 1.53±0.77, p<0.05). The means of tail moments also were compared among group I sprayers (n=36), group II sprayers (n=24) and, control subjects, and the means of tail moments were 3.45±3.20, 2.66±2.20 and 1.53±0.77 respectively. The difference between means of group I sprayers and controls was statistically significant (p<0.05). In conclusion, this study showed higher DNA damage in farmers exposed to pesticides than control subjects, and<br/> comet assay could be useful as a biological monitoring method of genotoxic pesticides for farmers.

드라이클리닝 근로자들의 유기용제 폭로와 자각증상

김수영,김정윤,이연경,이석구,이영수,조영채,이태용,이동배,Kim, Soo-Young,Kim, Jeong-Yun,Lee, Yeon-Kyeng,Lee, Sok-Goo,Lee, Young-Soo,Cho, Young-Chae,Lee, Tae-Young,Lee, Dong-Bae 대한예방의학회 1998 예방의학회지 Vol.31 No.4

To investigate the exposure levels of organic solvents and subjective symptoms of dry-cleaning workers, 77 male and 52 female dry-cleaning workers who had been worked in a small city of Chungnam province, and a large city, Taejon were selected for the study group. Air concentrations of organic solvents in the working environment were analyzed, and subjective symptoms of dry-cleaning workers were surveyed, from July to August 1996. The results obtained were as follows : 1. The concentrations of organic solvents in the working environment were within permissible TLV-TWA limits. 2. For the 13 symptom clusters, the most frequently complained symptom clusters were fatigue as 71.3%, and followed by depression and urinary disturbances as 53.5% and 51.9%. Other symptom clusters complained were below 50%. 3. Positive response rates of subjective symptoms were significantly higher in worker groups such as lived in a large city, female, higher education level, more frequently alcohol drinking, higher concentration of organic solvent in working environment, work in alone. 4. Workers who had used solvent B showed 2.3 point higher scores of subjective symptoms than those of solvent A. Of the subjective symptoms scores, amnesia and nervousness were higher in solvent B user group than solvent B user group. 5. As a result of factor analysis, 3 factors such as depression, urinary disturbance and neurologic disturbance were selected. 6. As a result of the logistic regression analysis, sex, the number of fellow workers, working time, region, job tenure, smoking, alcohol drinking, ventilating system, concentration of organic solvent in working environment and place of residence were selected for the related variables. For the conclusion, even though the concentrations of organic solvents in the working environments of dry-cleaning workers were within permissible limit of TLV-TWA, many dry-cleaning workers complained symptoms, such as fatigue, depression, urinary disturbances and so on. And the factors affecting to the symptoms of dry-cleaning workers were the number of fellow workers, work hours, region, job tenure, smoking and alcohol drinking.

일부 농촌지역 비닐하우스 재배자들의 농부증 실태와 관련요인

이인배(In-Bae Lee),이연경(Yeon-Kyeng Lee),장성실(Sung-Sil Chang),이석구(Sok-Goo Lee),조영채(Young-Che Cho),이동배(Dong-Bae Lee),이태용(Tae-Yong Lee) 한국농촌의학 지역보건학회 1999 농촌의학·지역보건 Vol.24 No.1

보건소 예방접종 전산프로그램의 운영 현황 분석

이건세 ( Kun Sei Lee ),이석구 ( Seok Goo Lee ),이무식 ( Moo Sik Lee ),신의철 ( Eui Chul Shin ),김영택 ( Young Taek Kim ),이연경 ( Yeon Kyeng Lee ) 한국보건행정학회 2003 보건행정학회지 Vol.13 No.2

Background: Immunization has been one of the most effective measures preventing from infectious diseases. However, children routine vaccination rate of Korea was 68.2% and it was not higher than expected. Korean government revised the School Health Law for every primary school children to submit the vaccination certificate record from 2005. It is quite important national infectious disease prevention policy to keep the immunizations rate high and monitor the immunizations rate continuously. To do this, National Institute of Health introduced the National Immunization Registry(NIR) Program at 2000. Objective: The aims of this study was to evaluate the Immunization Registry program which has been implementing since 2000 at the Public Health Centers(PHC). Methods: The mail survey was done from November 2001 to January 2002. 169 (69%) Public Health Centers among 244 PHC were responded. Results: The respondents of PHC said the Immunization Registry(IR) program had reduced the workload (18.5%). 69.2% said they inputted the immunization data into the IR program after the shots were given. 86.5% said they hadn`t checked or retrieved the children lists who had missed the scheduled immunization. Only 17.2% said the speed of internet for the IR program was good. It showed that 20% of respondents hadn`t written down documents, records on immunization any more. Even there were a lot of negative results, the respondents of PHC thought that the IR program was effective. They especially agreed that the IR program could make the job accurate (81.5%), convenient (71.3%), and reduced the chances of making mistakes (71.3%), increase the service quality (78.5%). And they were well adapting the job process of the IR (79.63%). Bivariate analysis showed that the software program was the important determinants of IR success. The only Bit Computer software program has been evaluated to be less satisfactory than the Integrated (Posdata operating system + Bit software) program. Other variables, such as age, duration of present job, and location of PHC (metropolitan, small city, rural area) were not significantly related. Conclusion: It seemed that the success of NIR might depend on the software program. Because Integrated program, which has been developed from 1994, include not only the general operating and management program for PHC but also IR program. It was natural to prefer Integrated program to Bit software program. So we can suggest that it is essential for the NIR to be successful that not only the immunization software program but also hardware equipments and public health information system should be further improved.

스마트폰을 이용한 예방접종 정보 모바일 서비스에 관한 연구

김창수,배근량,이연경,김명진,Kim, Chang-Su,Bae, Geun-Ryang,Lee, Yeon-Kyeng,Kim, Myong-Jin 한국정보통신학회 2010 한국정보통신학회논문지 Vol.14 No.11

예방정보 기록은 지역사회의 예방접종률을 파악하고, 예방접종의 효과를 모니터링 할 수 있으며, 또한 예방접종 사고시 원인조사의 근거로 사용할 수 있다. 따라서 예방접종 기록을 보다 효율적으로 관리하고 민간 및 공공기관에서 실시한 예방접종 정보가 통합적으로 관리할 수 있는 시스템의 개발이 필요하게 되었다. 이에 정부에서는 예방접종 기록 등록 전산화 사업을 추진하여 2009년 예방접종 등록 시스템 개발사업을 완료하였다. 예방접종 정보시스템의 사용자의 편의성 효율성을 고려한 다양한 사용자의 요구사항이 증가되고 있다. 이에 본 논문에서는 최근 스마트폰 보급확산 및 사용자 증가에 따라 예방접종 정보에 대한 사용자 편의성 및 접근성을 강화하고, 시간과 공간의 제약 없이 다양한 예방접종 정보를 활용할 수 있도록 스마트폰을 활용한 모바일 서비스를 설계 및 구현하였다. Immunization information recorded on the Immunization rate in the community to identify the effects of Immunization can be monitored Immunization during an accident can be used as a basis for investigating the cause. Thus, Immunization records, and more efficient management in the private and public institutions have conducted Immunization information systems to manage the development of integrated system has to be. So, the government projects that promote Immunization records were computerized registration. And, in 2009 the development of Immunization registration system was completed. In this paper, we use the information to a variety of Immunization using smart phone design and implement mobile service.

일부 청소년의 요중 코티닌 농도와 자매염색분체 교환빈도

백동기,이태용,노영만,이연경,조영채,이동배,장성실,Paek, Dong-Ki,Lee, Tae-Yong,Roh, Young-Man,Lee, Yeon-Kyeng,Cho, Young-Chae,Lee, Dong-Bae,Chang, Seong-Sil 대한예방의학회 2001 예방의학회지 Vol.34 No.3

Objectives : To evaluate the internal burden and hazardous effects associated with smoking in middle and high school students. Methods : We analysed urinary cotinine(U-cotinine) concentrations and the frequency of Sister Chromatid Exchanges (SCE). A comparison was done of U-cotinine concentrations and the frequency of SCE in peripheral lymphocytes across school levels (middle vs. high) and smoking types (direct: daily & occasional smoking, indirect; usual indirect & non-smoking), in 122 males. Results : The middle school student group comprised 6.8% daily smokers, 15.9% occasional smokers, 40.9% daily indirect smokers, and 35.4% nonsmokers, while the high school student group comprised 18.0%, 20.5%, 35.7%, and 21.8%, respectively. The U-cotinine concentration and the frequency of SCE among the middle school students were $79.11{\mu}g/l$ and 2.0 per cell, respectively, which were significantly lower than the $146.85{\mu}g/l$ (p=0.078) and 2.6 per cell (p=0.005) of the high school students. Among the 40 direct smokers, these two biomarkers were $236.66{\mu}g/l$ and 2.59 per cell, significantly higher than the $67.33{\mu}g/l$ (p=0.0001) and 2.1 per cell (p=0.003) among indirect smoking groups. The variation in individual U-cotinine concentration ranged widely in both the indirect and direct smoking groups. Conclusion : Urinary cotinine concentrations and the frequency of Sister Chromatid Exchange seem to objectively and effectively evaluate student exposure whether it was direct or indirect smoking. Consequently, these biomarkers may be useful in monitoring the objective efficacy of anti-smoking programs in adolescent populations.

충남 일부 농촌지역 주민들의 농부증에 관한 조사

송주영(Joo-Young Song),이연경(Yeon-Kyeng Lee),이석구(Sok-Goo Lee),이태용(Tae-Yong Lee),조영채(Young-Chae Cho),이동배(Dong-Bae Lee) 한국농촌의학 지역보건학회 1998 농촌의학·지역보건 Vol.23 No.1

건강면접조사의 현황과 발전 방향

강은정(Eunjeong Kang),김명희(Myoung-Hee Kim),이연경(Yeon-Kyeng Lee),최보율(Bo Youl Choi) 한국역학회 2007 Epidemiology and Health Vol.29 No.2

  The Health Interview Survey in the Korean National Health and Nutrition Examination Survey is a major source for planning and evaluating the national health promotion policy. The aim of the study is to find the areas for improvement of the current Health Interview Survey in light of its stated purpose and to propose possible means for the improvement. In terms of the survey content, there were several areas that needed improvement: the lack of comparability across the waves, the limit of the number of questions due to the Nutrition Survey and the Examination Survey, non-availability of seasonal statistics, and the dearth of evidence on socioeconomic position variables. To make improvements in the survey content, there is a need to clarify the purpose of the HIS in the KNANES. Second, more items need to be developed to produce the indicators of the Health Plan 2010. Third, core questions and elective questions should be defined. Fourth, multi-level socioeconomic position indicators need to be developed. In terms of the survey methods, the difficulty of managing interviewers and the inefficiency of data input and processing were found to be the areas for improvement. Possible solutions include a survey through the year and the introduction of the CAPI system. In addition, we suggest that a two-year survey period to better synchronize with the local health interview surveys which should be the major data source for the community health promotion plan.

일부 보건소에서 실시한 인플루엔자 예방접종 실태

임현술(Hyun-Sul Lim),배근량(Geun-Ryang Hae),민영선(Young-Sun Min),김영택(Young Taek Kim),이연경(Yeon Kyeng Lee) 한국역학회 2004 Epidemiology and Health Vol.26 No.2

Purpose: This study was conducted to improve the Korean influenza management system and to determine the status of influenza vaccination in some community health centers through a survey of the officers in charge of influenza vaccination. Methods: The authors conducted a questionnaire survey bye-mail for the officers in charge of influenza vaccination of 8 community health centers in Daegu-si, 5 community health centers in Ulsan-si and 25 community health centers in Gyeongsangbuk-do. The authors developed a questionnaire including selection methods of the influenza vaccination objects, results of influenza vaccination in 2002, problems of influenza vaccination, the population within the respondents' jurisdiction and so on. Results: The proportion of influenza vaccination at community health centers among all residents in the 2002-2003 influenza season was 3.8% in Daegu-si, 14.8% in Ulsan-si, 15.1% in urban Gyeongsangbuk-do and 24.9% in rural Gyeongsangbuk-do, The proportion of influenza vaccination at community health centers among priority cases for vaccination was 12.9% in Daegu-si, 43.1% in U1san-si, 39,3% in urban Gyeongsangbuk and 41.6% in rural Gyeongsangbuk-do. The officers in charge of influenza vaccination stated that the problems of influenza vaccination were deficiencies in preparatory examination such as manpower shortage. Twenty-five persons stated as a problem that influenza vaccinations conducted in the hospitals were hardly reported, and fifteen of these clarified that this was due to hospital indifference. Conclusions: The authors examined all community health centers of Daegu-si, Ulsan-si, and Gyeongsangbuk-do, and investigated the vaccination states and problems that health officers were dealing with. Therefore, this study is meaningful with its basic data for the management of domestic influenza vaccination.

일부 농촌지역 주민들의 인플루엔자 예방접종 실태

임현술(Hyun-Sul Lim),민영선(Young-Sun Min),배근량(Geun-Ryang Bae),김영택(Young-Taek Kim),이연경(Yeon-Kyeng Lee) 한국농촌의학 지역보건학회 2004 농촌의학·지역보건 Vol.29 No.1