적합수혈후 초래된 지연성 용혈작용이라 추정한 1 례 보고

이신우,김병권,이정웅,박무길 대한마취과학회 1975 Korean Journal of Anesthesiology Vol.8 No.1

Hemolytic transfusion reactions may occur after the administration of donor bloods even when they have been shown compatible apparently by crossmatch tests. Such episodes present a diagnostic challenge and raise serious doubts about our understanding of blood group incompatibility. Fever, hemoglobinuria, oliguria and marked fall in hematocrit values developed in a patient 30 hours after she had received one unit of apparently compatible blood during operation under general anesthesia. Results of routine serologic studies at the time of the transfusion reaction were normal. She died on 40 hours after operation.

국내·외 GM식물의 개발 및 산업화 현황

이신우,Lee, Shin-Woo 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.3

지난 십년까지 상업화가 승인된 GM작물들의 특성을 보면 첫 단계에는 제초제저항성, 해충저항성, 바이러스 저항성 유전자가 도입된 작물 등으로 농업적 생산성을 증가시키기 위한 단일유전자가 도입된 것들이었으나 최근 들어 지방산 조성을 변경하거나, 특정 필수아미노산의 함량을 증가시키거나, 또는 화색을 변경 시킨 제 2세대 GM식물들의 승인건수가 점차 증가하는 추세에 있다. 뿐만 아니라, 현재 국가별로 심사 중에 있거나 환경위해성 평가용 자료를 생산하기 위하여 제한된 구역에서 포장시험을 수행하고 있는 것으로 알려진 GM 작물들을 보면 가뭄, 냉해 등에 내성이 강한 무생물적 스트레스 내성 작물, 녹색 성장의 주역이 될 biomass 증가, 또는 바이오디젤연료 생산 작물, 그리고 각종 의료용 및 산용 특수물질을 생산하는 분자농업용 작물 등이 주를 이루고 있는 것으로 조사되었다. 또한 아직은 실험실 또는 연구실 수준에서 성공하여 전문 학술지 또는 다양한 언론매체를 통하여 보도된 자료들을 분석한 결과에서도 이러한 차세대 GM 작물들의 개발건수가 현저하게 증가하고 있다. 특히 분자농업용 GM작물의 개발 건수가 가장 많은 것으로 조사되었다. 국내의 경우에도 비슷한 추세로 다양한 연구과제가 진행 중에 있는 것으로 조사되었으며. 국내에서 개발되어 최초로 환경위해성 평가를 신청한 GM 작물은 제초제 저항성잔디로서 현재까지 심사가 진행 중에 있으며, 이외에도 향후 신청을 위하여 포장시험이 진행 중에 있는 GM 작물들이 벼, 고추, 배추 등 약 10여종으로 이들 중 벼에 관한 건수가 가장 많았으며 그 중에서도 흑명나방 저항성 벼가 가장 많은 연구가 진행되어 곧 환경 위해성 평가를 신청할 것으로 조사되었다. During a last decade, the introduced traits in commercialized GM crops have been diversified from a simple trait such as herbicide resistance gene or insectresistance gene which are related to the crop production into more complicated traits such as modification of fatty acid or essential amino acid composition, modified coloring pattern of flower. In addition, it was investigated that several other GM crops bearing more refined traits expected to lead next generation are also awaiting for risk assessment (RA) or under field test for the preparation of RA in the near future. These GM crops include abiotic stress resistance including drought or cold, increased biomass, production of bioethanol or diesel, production of pharmaceuticals or functional materials for industrial. In particular, in 2008 and 2009, it was reported that the highest number of GM crops for molecular farming are under developed in laboratory or green house level in all the world. Likewise, in Korea, 171 events from 49 plant species are under developed to introduce several important traits. At present, about 10 events are under field test to select elite lines for RA application. For the first time, herbicide resistance turfgrass developed by Korean research team has been submitted for RA and currently under requested for additional data. Moreover, GM rice resistant to leaf roll (folder) disease is expected as a next event to be submitted for RA application.

리게티 음악에 나타난 아프리카 음악의 영향 : 피아노 에튀드 제 1 권을 중심으로 A Study of Etudes pour piano, premier livre

이신우 서울대학교 서양음악연구소 2000 음악이론연구 Vol.5 No.-

Ligeti's interest in African Music, particularly in its polyrhythmic structure, initially began in 1982 when his former student Roberto Sierra introduced him a collection of instrumental and vocal ensemble music of the Banda-Linda tribe from the Central African Republic, recorded by the Ethnomusicologist Shimha Arom. During the spring of 1984, while Ligeti was in Jerusalem, he met Shimha Arom, at which time he had an opportunity to see Atom's transcriptions in full score of sub-Saharan music and to hear about their melodic and rhythmic structure. Six short pieces in Piano E´udes Book I (1985) are his first musical products written after his serious encounter with African Music, and those six pieces explicitly show considerable features of being influenced by African Music. African systems of timing are based on a number of certain principles that cannot be equated with Western metrical systems: (1) unchanging repetition of cycles of equal length, (2) asymmetrical internal divisions of periods (metrical units), (3) elementary units as a mental background pulsation. Following these principles of timing, Central African Music is proceeded in highly complex polyrhythmic polyphony, which can be more specifically defined as ostinatos with variations, imitation, melodic and rhythmic counterpoint and the hocket technique. This paper examined the influence of Central African Music on Ligeti's Piano E´tudes Book I. Some characteristics of Central African Music including the above were discussed, in order to explain how Ligeti applied African polyrhythmic features into his Piano E´tudes Book I. The second movement of Ligeti's Trio fur violine, horn und klavier (1982) was also briefly studied to demonstrate its further changes of metrical systems in Piano E´tudes Book I.

외래 DNA단편이 잔존하지 않는 유전자교정식물에 대한 GMO규제 범위의 제외에 관한 국제 동향

이신우 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.3

Gene edited crops can be classified as SDN-1, SDN-2 and SDN-3 group depending on their mutation’s range and the usage of donor DNA. The SDN-1 and SDN-2 crops, in particular, could be developed as 100% transgenefree, which do not contain any DNA fragment of the vector or guide RNA used for gene editing such as CRISPR Cas9 system. Therefore, there are no scientific methods available for the detection of these crops and differentiation with the one produced by conventional cross breeding techniques. Additionally, it would be impossible to properly implement the existing GMO regulation law, in particular, the national legislation for “GMO labelling”. In this regard, Australia has announced that SDN-1 crops will not be subjected to the existing GMO regulation. Furthermore, Argentina and Brazil have established a new policy that GE crops with no transgene (100% transgene-free crops) should be exempted from the scope of the GMO. In addition, Japan has also announced that “an organism that has no remnants of inserted nucleic acid processed extracellularly is not subjected to the Cartagena Act”. It means that SDN-2 crops can also be exempted from the scope of GMO. In this trend, in South Korea, I suggested that gene edited crops with no remnants of inserted foreign DNA fragments should be excluded from the existing GMO regulation. Thus, I expect that diverse elite crop lines should be developed by using advanced gene editing technologies.

Erratum: 진보된 DNA barcoding 기술을 이용한 당귀(Angelica)속 식물의 기원 판별 기술에 관한 연구 동향

이신우,신용욱,김윤희,Lee, Shin-Woo,Shin, Yong-Wook,Kim, Yun-Hee 한국식물생명공학회 2022 JOURNAL OF PLANT BIOTECHNOLOGY Vol.49 No.1

글로벌 GM 작물 실용화를 위한 중국의 GM 작물 안전관리제도 분석

이신우,조광수,왕지,곽상수 한국식물생명공학회 2012 식물생명공학회지 Vol.39 No.3

We analysed the current status of development of GM crops and national biosafety framework including legislation-related agricultural GMO in China to provide the policy for the development of global GM crops in Korea. In China, several GM crops including cotton, petunia, tomato, sweet pepper, poplar, and papaya have been approved for commercialization and they have been cultivated at more than 4 million ha. In addition, GM rice and GM maize have also obtained approval for productive testing in 2009. China will be the first country to approve GM rice for commercialization. Prior to commercialization in China, all GM crops must be approved by government authority for biosafety assessment specified by national legislation including restricted field testing, enlarged field testing, productive testing and safety certificate. According to China’s legislation, agricultural GMOs have been classified by research and testing, production and processing. All GMOs must go through 3 steps of field testing (restricted, enlarged and productive). Prior to conducting each field testing, it has to be approved by government authority. It is assumed that at least one to two years will be taken for each step of field testing (total 4 to 8 years to obtain the final safety certificate) along with a large amount of budget. 중국은 GM 면화, 페튜니아, 토마토, 피망, 포플러, 파파야 등의 상업화를 승인하여 그 재배면적도 1996년부터전 세계에서 6위를 차지하고 있다. 특히 지난 2009년에GM 벼와 GM 옥수수의 대규모 포장시험이 허가된 이후밀과 함께 주곡 작물에 대한 연구가 활발하게 진척되고있어 향후 중국에서 가장 먼저 GM 쌀의 상업화가 승인될 것이라 전망된다. 현재까지 상업화가 허가된 대부분의 GM 작물들은 중국에서 자체적으로 개발된 것으로 중국의 농업용 GMO 안전관리법령에 따라 소규모 포장시험, 중간규모 포장시험, 대규모 포장시험단계를 순서대로 허가를 얻어 수행한 실험결과를 바탕으로 안전하다는결론에 도달하여 상업화를 위한 안전증명서를 발급받은GM event이다. 법령에 의하면 외국에서 수입하고자 하는농업용 GMO는 연구와 시험, 생산, 가공용 등 3가지 그룹으로 구분하고 상업용으로 수입되는 농업용 GMO 역시3가지 포장시험 즉 소규모, 중간단계, 대규모 그리고 마지막 안전증명서를 발급받는 단계별 승인절차를 거쳐야하는 것으로 조사되었으며 초기단계부터 신청서를 제출하여 승인을 얻어야 시험용 시료의 반입이 허용된다. 이러한 단계별 포장시험에 소요되는 기간이 최소한 각 단계별로 1 ~ 2년 이상 소요되어 외국에서 개발된 GM 작물이 중국에 반입되어 재배 등 상업화가 되기까지는 상당한 시간과 예산이 소요될 것으로 전망된다. 본 논문에서는 중국의 안전관리체계와 국내의 안전관리체계와 비교하여 크게 차이가 나는 단계별 포장시험의 구분체계, 허가절차 등을 세밀하게 검토하여 향후 국내에서 개발 중에 있는 global GM 작물의 실용화에 참고자료로 활용하도록 하였다.

유전자편집 작물의 개발 현황 및 농업생명공학기술의 국가 경쟁력 강화

이신우 한국식물생명공학회 2018 JOURNAL OF PLANT BIOTECHNOLOGY Vol.45 No.3

In this paper, mechanisms of gene editing technologies including ZFN, TALENS and CRISPR were briefly discussed with mutual advantages and disadvantages. Classification criteria of gene edited, site-directed mutagenesis (SDN) crops for regulatory purpose were also discussed. The number of studies using CRISPR technology was high and studies conducted on Arabidopsis thaliana and rice were highest, followed by tobacco, tomato, wheat, and corn. It has been applied to a variety of plants such as other grain crops, flower crops, vegetable crops, and fruit trees. The number of studies focused on practical application or commercialization in the future were also increasing yearly, and the scope of studies also expanded to include research on metabolic engineering for mass production of useful proteins or substances, development of disease resistant crops against viruses, bacteria, and fungi, abiotic environmental stressresistant crops, and increased yields. In addition to this, it was revealed that application range is becoming more diversified, including the development of parthenocarpic tomatoes, hybrid rice lines using male sterility and increased shattering resistance Brassica napus. It was also revealed that the number of CRISPR gene edited crops permitted by the USDA(APHIS) increases yearly, to be released in the international seed market soon.

유전자교정작물 내 비의도적 돌연변이의 안전성 논란에 관한 과학적 고찰

이신우,김윤희 한국식물생명공학회 2020 JOURNAL OF PLANT BIOTECHNOLOGY Vol.47 No.3

The number of commercially approved gene-edited crops is gradually increasing, and in South Korea, it has led to intense investment in gene-edited crop development to increase international competitiveness. However, as with genetically modified crops, the safety of gene-edited crops regarding unexpected risks for humans and the environment is subject to an ongoing debate. In particular, unintentional “off-target effects” have become the center of controversy. In this review, we discuss typical plant characteristics (including somatic variation and ploidy), the extent of various off-target effects in genetically modified crops generated via horizontal transfer in nature, and the off-target effects in commercial genetically modified crops. We conclude that most off-target effects possibly occurring in gene-edited crops are not expected to be critically harmful to humans or the environment. Therefore, existing regulation for genetically modified crops should be enough for the risk assessment of gene-edited crops.

한성부의 ‘작은 일본’, 진고개 혹은 本町 : 서울 근대 도시사 연구의 새로운 장을 열다.

이신우 인천대학교 인천학연구원 2016 인천학연구 Vol.24 No.-

국내・외 GM식물의 개발 및 산업화 현황

이신우 한국식물생명공학회 2010 JOURNAL OF PLANT BIOTECHNOLOGY Vol.37 No.3

During a last decade, the introduced traits in commercialized GM crops have been diversified from a simple trait such as herbicide resistance gene or insectresistance gene which are related to the crop production into more complicated traits such as modification of fatty acid or essential amino acid composition, modified coloring pattern of flower. In addition, it was investigated that several other GM crops bearing more refined traits expected to lead next generation are also awaiting for risk assessment (RA) or under field test for the preparation of RA in the near future. These GM crops include abiotic stress resistance including drought or cold, increased biomass, production of bioethanol or diesel, production of pharmaceuticals or functional materials for industrial. In particular, in 2008 and 2009, it was reported that the highest number of GM crops for molecular farming are under developed in laboratory or green house level in all the world. Likewise, in Korea, 171 events from 49 plant species are under developed to introduce several important traits. At present, about 10 events are under field test to select elite lines for RA application. For the first time, herbicide resistance turfgrass developed by Korean research team has been submitted for RA and currently under requested for additional data. Moreover, GM rice resistant to leaf roll (folder) disease is expected as a next event to be submitted for RA application. 지난 십년까지 상업화가 승인된 GM작물들의 특성을보면 첫 단계에는 제초제저항성, 해충저항성, 바이러스저항성 유전자가 도입된 작물 등으로 농업적 생산성을증가시키기 위한 단일유전자가 도입된 것들이었으나 최근 들어 지방산 조성을 변경하거나, 특정 필수아미노산의 함량을 증가시키거나, 또는 화색을 변경 시킨 제 2세대GM식물들의 승인건수가 점차 증가하는 추세에 있다. 뿐만 아니라, 현재 국가별로 심사 중에 있거나 환경위해성평가용 자료를 생산하기 위하여 제한된 구역에서 포장시험을 수행하고 있는 것으로 알려진 GM 작물들을 보면가뭄, 냉해 등에 내성이 강한 무생물적 스트레스 내성 작물, 녹색 성장의 주역이 될 biomass 증가, 또는 바이오디젤연료 생산 작물, 그리고 각종 의료용 및 산용 특수물질을 생산하는 분자농업용 작물 등이 주를 이루고 있는 것으로 조사되었다. 또한 아직은 실험실 또는 연구실 수준에서 성공하여 전문 학술지 또는 다양한 언론매체를 통하여 보도된 자료들을 분석한 결과에서도 이러한 차세대GM 작물들의 개발건수가 현저하게 증가하고 있다. 특히 분자농업용 GM작물의 개발 건수가 가장 많은 것으로조사되었다. 국내의 경우에도 비슷한 추세로 다양한 연구과제가 진행 중에 있는 것으로 조사되었으며. 국내에서 개발되어 최초로 환경위해성 평가를 신청한 GM 작물은 제초제 저항성잔디로서 현재까지 심사가 진행 중에있으며, 이외에도 향후 신청을 위하여 포장시험이 진행중에 있는 GM 작물들이 벼, 고추, 배추 등 약 10여종으로이들 중 벼에 관한 건수가 가장 많았으며 그 중에서도 흑명나방 저항성 벼가 가장 많은 연구가 진행되어 곧 환경위해성 평가를 신청할 것으로 조사되었다.