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      • KCI등재

        도시형 한옥주거지의 물리적 변화에 따른 사회적 특성변화

        이승엽,Lee Seung-Yeob 한국주거학회 2006 한국주거학회 논문집 Vol.17 No.2

        Korean urban traditional housing abandoned by the social apathy promptly disappearing form our sight, or ruining. It tells that the strategies we are using today are impossible to fulfill socio-economic needs of residents here. So it needed to find out the strategies which can change this place better to live in. Consolidating the street parking lots fer cars can be one of the important solutions to the area. By using cul-de-sac, after setting up small unit of communities, we will have chance to have a small public space, and at the same time it can help to set up our tradition of people's intimacy inside the community. Most people have their own cars, and the need of parking lots becomes more serious. So, a meeting place like public well in the past must replace parking lots. On the other hand, it is important to re-consolidate existing land to obtain appropriate land size for other uses. Today, the use of private space (house, private lots, etc..)and public space(streets, local offices) is very important. NIMBY (Not In My Back Yard) attitude should also be reconsidered. All these negative social aspects come from negative social relationship, thus we should consider them with care and deep understandings. The traditional housing of Korea should not be abandoned as slum, but this should be transformed in terms of the conservation since it is superior in its ecological and energy conservation aspect.

      • KCI등재

        털머위 (Farfugium japonica)의 캘러스 유도 및 식물체 분화에 미치는 생장조절제의 영향

        이승엽,유성오,배종향,이중호,Lee, Seung-Yeob,Yoo, Sung-Oh,Bae, Jong-Hyang,Lee, Joong-Ho 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.1

        털머위의 조직배양에 의한 식물체의 기내 미세증식을 위하여, 잎과 잎자루 조직으로부터 캘러스 유도 및 식물체 분화에 미치는 몇 가지 생장조절제들의 영향을 조사하였다. 캘러스 유도 및 생장은 잎과 잎자루 조직 모두 1∼2 mg/L 2,4-D와 1∼2 mg/L BA의 혼합배지에서 양호하였으며, 치상 조직간 캘러스 유도율은 비슷하였으나, 캘러스 형성시기는 잎절편에서 보다 잎자루 조직에서 더 빠르고 왕성하였다. 캘러스로부터 식물체 분화를 위한 적정 생장조절제의 농도는 1 mg/L NAA와 2 mg/L BA 혼합배지였다. 분화된 식물체는 캘러스와 함께 동일배지에 60일 정도 계대배양하면 다아체를 형성하였다. 분화된 유식물체는 0.5 mg/L IAA를 첨가한 MS배지에 옮겨 30일간 생육시킨 후, 초장 50 mm 이상의 소식물체를 vermiculite에서 순화시켰을 때 생존율은 95% 이상으로 높았으며, 토양활착도 잘 되었다. The leaf and petiole segments of Farfugium japonica were cultured to investigate the influence of growth regulators on their callus induction and plant regeneration. The callus induction and growth showed a good response both leaf and petiole on MS media supplemented with 1∼2 mg/L 2,4-D and 1∼2 mg/L BA. Callus induction and growth were more effective in petiole segments than leaf one. The highest percentage of plant regeneration was obtained from 60-day-old calli on MS medium supplemented with 1 mg/L NAA and 2 mg/L BA. When subcultured to the same medium for about 60 days, multiple shoots were developed from regenerating callus. The shoots produced roots after transferring to rooting medium containing 0.5 mg/L IAA. The plantlets over 50 mm in height were successfully acclimatized in vermiculite, and the survival rate was over 95%.

      • KCI등재

        네리네(Nerine bowdenii)의 기내 인편배양시 자구형성에 미치는 생장조절제와 Sucrose 농도의 영향

        이승엽,안정호,박윤점,Lee, Seung-Yeob,Ahn, Jeong-Ho,Park, Yun-Jum 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.2

        네리네의 인편 배양에 의한 기내 증식을 위하여, 쌍인편으로부터 자구형성에 미치는 NAA와 BA 및 sucrose 농도의 영향을 조사하였다. 네리네의 쌍인편으로부터 기내 자구형성에 가장 적합한 배지는 1.0mg/L NAA와 2.0mg/L BA를 첨가한 MS배지였다. 적정 sucrose 농도는 30 g/L에서 가장 양호하였으며, 90g/L 이상의 sucrose 첨가배지에서의 자구형성은 심하게 억제되었다. 비대된 인편을 단축경과 함께 5mm크기로 잘라 60일 간격으로 1, 2, 3차 계대 배양한 결과, 절편당 자구수는 6.5, 7.3, 8.2개로 다수의 기내자구를 지속적으로 생산할 수 있었다. 기내 형성된 3mm 이상의 자구는 생장조절제를 첨가하지 않은 MS배지에 옮겨 60일간 생육시킨 후, 초장 50mm이상 자란 식물체를 버미큐라이트와 펄라이트를 1:1 혼합한 배양토에 순화시켰을 때, 생존율은 95% 이상으로 높았다. The twin-scale segments of nerine (Nerine bowdenii) were cultured to investigate the influence of NAA, BA and sucrose concentrations on in vitro bulblet formation. The formation of bulblets from twin-scale segments showed a good response both the percentage of bulblet formation and the number of bulblets per explant on MS medium supplemented with 1mg/L NAA and 2 mg/L BA. Formation of bulblet showed the highest efficiency on medium containing 30g/L, and the formation of bulblets was strongly inhibited on medium containing over 90g/L. When the twin-scale segments formed bulblets were subcultured three times to the same medium by 60 day subculture interval, the number of bulblets per explant was 6.5, 7.3 and 8.2 in order of first, second and third. The bulblets over 3mm in diameter were hypertrophied and rooted after transferring to the hormone-free MS medium. The plantlets over 50mm in height were successfully acclimatized in the soil mixed with the same volume of vermiculite and perlite, and the survival rate was over 95%.

      • KCI등재

        효율적인 수은이온 검출을 위한 피렌-보론산 기반의 형광센서 개발

        이승엽,이성호,Lee, Seung Yeob,Lee, Seoung Ho 한국환경과학회 2020 한국환경과학회지 Vol.29 No.2

        A new chemosensor based on a self-assembled system has been devised to detect Hg<sup>2+</sup>ions efficiently. We demonstrated that the amphiphilic building blocks consisting of pyrene and boronic acid (1) aggregate in aqueous solutions and provide an outstanding sensing platform for sensitive detection. The self-assembled 1 exhibited high selectivity and sensitivity for Hg<sup>2+</sup>ion detection via fluorescence quenching, where the Hg<sup>2+</sup>ion detection ensued from a fast transmetallation of 1. The Stern-Volmer (SV) quenching constant for its fluorescence quenching by Hg<sup>2+</sup>ions was approximately 1.58 × 10<sup>8</sup> M<sup>-1</sup>. In addition, self-assembled 1 exhibited excellent sensing abilities at nano-molar concentration levels when tap water and freshwater samples were contaminated with of Hg<sup>2+</sup> ions.

      • KCI등재후보

        벼 組織培養 由來 變異系統의 主要特性 및 種子 蛋白質 電氣泳動相

        Seung Yeob Lee(李承燁),Jae Kil Lee(李載吉),Hyun Jung Kang(姜鉉中),Hyun Tak Shin(申鉉卓),Seon Yong Lee(李善龍) 한국육종학회 1995 한국육종학회지 Vol.27 No.3

        To select the useful rice variants by tissue culture, anther, isolated microspore or inflorescence of four japonica rice cultivars were cultured in vitro. Regenerated plants showed wider range of variation than original cultivar in several characteristics. 18 variants were selected from the progenies(R₂) derived from anther(AC), isolated microspore(MC) or inflorescence culture(IC). The selected variants(R₂) were investigated for agronomic characteristics and grain quality. Five AC lines derived from Koshihikari were delayed for 4~10 days in heading date than original cultivar. Culm length and panicle length were simultainously reduced in most of the selected lines, while Dongjinbyeo-IC 1 and Norinmochi No. 1-AC 13 were semidwarf without reducing the panicle length. Daecheongbyeo-MC 2, -MC 3, -MC 13 and -MC 21 were more highly tillered than original cultivar. Grain number per panicle of five variants, Dongjinbyeo -AC 7, -IC 1, Daecheongbyeo -MC 3, -MC 13 and -MC 21, was significantly increased and the highest one was 103 grains per panicle in Dongjinbyeo-IC 1. One-thousand-grain weight was significantly reduced in most of the variants except Daecheongbyeo-AC 11 and-AC 13. Milled rice yield of Daecheongbyeo-MC 3, and -MC 21 were significantly increased compared with the original cultivar, but that of other lines was the similar or reduced one. Amylose content and alkari digestibility of selected lines were similar to those of original cultivars. Chalkiness of milled rice were more increased in Dongjinbyeo-IC 1, Koshihikari-AC 3, -AC 4 and -AC 7, and Daecheongbyeo-MC 3 compared with the original cultivars. SDS - PAGE banding patterns of seed protein showed no differance between variants and original cultivars. The specific band(pH 4.2~4.8) of esterase in Norinmochi No. 1-AC 13 was stronger than that in original cultivar, and the band wasn’t detected in non-glutinoues cultivars, Dongjinbyeo and Daecheongbyeo.

      • KCI등재

        토경 및 수경재배에서 돌나물의 생육, 비타민 C와 무기성분 함량 변이

        이승엽(Seung-Yeob Lee),김효진(Hyo-Jin Kim),배종향(Jong-Hyang Bae) 한국원예학회 2011 원예과학기술지 Vol.29 No.3

        여름철 고품질 돌나물(Sedum samentosum)을 생산하기 위하여, 4지역의 수집종에 대한 토경재배와 NFT 수경재배에 따른 생육특성, 수량 및 품질, 비타민 C와 무기성분 함량 등을 조사하였다. 초장은 18.4-21.8㎝ 범위로 재배방식 간에 유의한 차이를 보이지 않았다. 개체당 신초수, 엽장, 엽폭, 엽수, 생체중 및 건물중 등은 토경재배보다 수경재배에서 유의한 증가를 보였으며, ‘군산’, ‘완도’ 수집종에서 단위 면적당 생체중 및 건물중이 높았다. 줄기두께는 수경재배보다 토경재배에서 증가하였으며, 수집종간에도 유의한 차이를 보였다. 줄기의 경도는 수경재배(1.3㎏ · ㎝?²)보다 토경재배(2.0㎏ · ㎝?²)에서 유의하게 높았으나, 수집종 간에는 차이를 보이지 않았다. 관능검사(1-9)에 의한 쓴맛은 보통이 었으며, 쓴맛 정도와 비타민 C 함량은 재배방식 간에 유의한 차이를 보이지 않았다. 무기성분 함량은 Fe를 제외한 대부분이 토경재배보다 수경재배에서 높은 경향이었으며, K, Na, Zn 함량은 2배 이상 높았다. The growth, yield and quality of Sedum sarmentosum between soil and hydroponic cultivation were compared to produce high-quality shoots in summer season. The plants were collected from four places in Korea and cultivated in a plastic film house with 50% shading. A 1:1:1 mixtures of sand, upland soil and substrate (N-P-K: 330-220-400 ㎎ · ℓ?¹) was used for soil cultivation, and a nutrient film technique (NFT) with Yamazaki-lettuce nutrient solution used for hydroponics. After 30 days of cultivation, plant height ranged from 18.4 to 21.8 ㎝ and showed no significant difference between soil and hydroponic cultivation. Number of shoot, leaf length, leaf width, number of leaf, fresh weight, and dry weight significantly increased in hydroponics compared to those in soil cultivation. Fresh and dry weights in hydroponics were greater in ‘Gunsan’ and ‘Wando’ collections than those in other collections. Stem diameter and compression-force significantly increased in soil cultivation rather than hydroponics with significant differences among collections. However there were no significant differences in shoot bitterness and vitamin C content between cultivation methods or among the collections. Most mineral contents except Fe in hydroponics were higher than those in soil cultivation. K, Na, Zn contents in hydroponics were over 2 times higher than those in soil cultivation.

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