오마환(烏麻丸)이 노인(老人)의 면역기능(免疫機能)에 미치는 영향(影響)

이송실,이상재,김광호,Lee Song-Shil,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2000 대한예방한의학회지 Vol.4 No.2

BACGROUND: To examine effect of omahwan(烏麻丸)-赤何首烏(Polygonum multiflorum THUNB.), 白何首烏(Cynanchum wilfordii H.), 黑芝流(Sesamum indicum L.)-on the aged human immune system a series of humoral immunological parameters was compared in aged female (age 60-70 years) before and after administration of omahwan(烏麻丸). METHODS: Peripheral blood was obtained from fifteen healthy young (age 20-29, mean=66.3) female volunteers. B cell, T cell(T cells, T helper, and T suppressor/ cytotoxic) subsets was examined with specific monoclonal antibodies and isotype controls, using dural color flow cytometer IL-2 was examined with ELISA kit RESULT: By comparing the immune characteristics of the younger and elder groups the total ratio of T-cell, CD8 T-cell and quantify of IL-2 was significantly lower while CD4/CDB ratio was considerably greater in the elder group. ( p(0.05, student t-test) After giving Omahwan for 30 days to the elder group, by comparing the ratio before and after Prescription the total T cell and CD8 T cell ratio was considerably greater after prescription (p(0.05, Paired t-test) The quantity of IL-2 tended to increase after prescription but has no statistical meaning. CONCLUSION: The results indicate that administration of Omahwan(烏麻丸) differentially affects various aspects of the immune system in aged human.

교등원(交藤圓)이 백서(白鼠)의 산화유발(酸化誘發)을 방어(防禦)하는 작용(作用)에 관(關)한 연구(硏究)

이송실,이상재,김광호,Lee Song-Shil,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2004 대한예방한의학회지 Vol.8 No.2

Objectives : JianTeng-Yuan(交藤圓) is said to be a prescription for preservation of health in ${\ulcorner}$HuaTuo ZhongZangJing(華陀 中藏經)${\lrcorner}$. It is known to have the effect of Bu-Shen(補腎: strengthening kidney) and Yi-Shou(益壽: prolonging the span of one's life). This study investigates whether JTY is effective on inhibition of oxidation stress. Methods : Sprague-Dawley Rats(12-week-old, weight $300{\pm}20g$) were divided into 3 groups. Normal group(n=8) was injected PBS(1ml/body, s.c) at the back neck's skin. Control group(n=8) was injected D-galactose(50mg/kg, 1ml PBS/body, s.c) to induce pathological animals. JTY group was injected the same treatment for the Control group, and fed containing JTY(10%). The whole groups were treated 1 time per day for 6 weeks. After rats were sacrificed and anti-oxidant enzyme(SOD, CAT, G-px) activity, GSH quantity of RBC and tissue(heart, liver and kidney), plasma Vit-C quantity were examined. Besides, the MDA levels of liver and kidney, lipofuscin of heart and endurance of erythrocyte membrane were measured. Results : In the JTY group, RBC's SOD activity decline was halted by 21% of the normal level, compared to the control group ; G-px activity(unit/g of Hb) increased significantly, compared to the normal group ; and the level of Vit-C in plasma increased by 16%. Heart's SOD activity was kept at the same level as that of the normal group ; and CAT activity decline was halted by 26%. Kidney's CAT and G-px activities were kept at the same level as that shown in the normal group, implying the existence of halting effect. Liver also showed a slight halting effect against the decline of anti-oxidant ability, but the effect was not significant(a=0.05). A comparison between the levels of peroxide in SD rats showed that the level of TBARS in plasma increased significantly in the control group and that it was normal in the JTY group. The livers in the JTY group, compared to those in the control group, showed 36% halting effect of the normal level while their kidney's indicated the level significantly lower than the normal level. Heart's lipofuscin increased significantly in the control group, but was alike in both the JTY and the normal groups. Endurance of erythrocyte membrane(%) decreased significantly in the control group while it was kept at the similar level in both the JTY and the normal groups, indicating the halting effect. Conclusions : This study suggests that JTY is effective to defend oxidation stress caused by D-galactose in the animals. It showed that the anti-oxidant ability was maintained and strengthened. On the other hand, it reduced the level of peroxide in animals. In sum, JTY appeared to have the equilibrium normal physiological function in SD rat.

오마환(烏麻丸)이 백서(白鼠)의 혈액내(血液內) CD4+, CD8+ T cell 및 면역기관(免疫器官) 장기지수(臟器指數)에 미치는 영향(影響)

이송실,이상재,김광호,Lee Song-Shil,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2001 대한예방한의학회지 Vol.5 No.1

OMH which is known for its properties of recruiting vitality, is composed of Polygonum multiflorum Thunb. and Sesamum indicum DC.. This formula is known to possess the properties of recruiting vitality, blackening white hair and expanding life span. 16-week-old SD-Rats were treated with OMH for 16 days. After 24 hours, the rats were treated with MTX(Mthotrexate is oral administrated for 4 days(1mg/kg/day), in order to lower immunity. Then, These rats were classified in to groups, the N-16 group(not specially tested), the Control group(MTX), the OMH-L group(2.5% OMH+MTX)&the OMH-H group(10% OMH+MTX) and 6 rats were assigned to each group. After 18 hours from MTX treatment the organ index of the rats from each group Thymus and Spleen were measured. The percentage of CD+4, CD8+ T cell were measured and compared by flow cytometer. 1) Rats from the OHM-L&OMH-H group showed higher organ indexes of the Thymus and Spleen compared to the rats from the Control Group. This proves that OMH possesses the properties to mitigate degenerations of immunity($F_{thymus}=20.162,\;F_{spleen}=5.882$, ANDVA, p<0.05). 2) The rats from the two OMH groups showed higher rates of CD4+ T cell counts compared to the control group(F=26.906, ANOVA, p<0.05). CD8+ T cell showed lower rates compared to the Control group, but showed no differences within the two OMH groups(F=1.254, ANOYA, p>0.05). CD4+/CD8+ showed higher rates in the two OMH groups compared to the control group which can be thought as a proof that OMH prevents depression of immune response(F=10.554, ANOVA, p<0.05). In this test 16 week-old rats were used, which can be considered as the middle and prime age of the human being. These rats were treated with OMH which ended up showing properties of mitigating degeneration of immune responses and maintaining T-cell rates within the blood. It was possible to study that OMH possesses the properties to increase immune responses.

한약물을 이용한 혈관신생 촉진에 대한 최근의 연구동향

이송실,강중원,백용현,최도영,박동석,김덕윤,김강일,박상도,양하루,지미영,이제동,Lee, Song-shil,Kang, Jung-won,Back, Yong-hyeon,Choi, Do-young,Park, Dong-seok,Kim, Deog-yoon,Kim, Kang-il,Park, Sang-do,Yang, Ha-ru,Ji, Mi-young,Lee, Jae-do 대한침구의학회 2004 대한침구의학회지 Vol.21 No.3

Background : Angiogenesis is the proliferation of a network of blood vessels emanating from pre-existing vessels, supplying nutrients and oxygen and removing waste products. Angiogenesis occurs in a variety of normal physiologic and pathologic conditions and is regulated by a balance of stimulatory and inhibitory angiogenic factors. Excessive angiogenesis should be suppressed. However, if blood supply is insufficient, it should be encouraged. Hyul-Mek(血脈) or Hyul-Rark(血絡), known as blood vessels in western medicine, is deeply related to Chung-Ki-Hyul(精 氣 血). The goal of this study is to review the effects of herbal medicines on angiogenesis that is involved in wound healing and enhancement of blood supply. Methods : We conducted a systematic and comprehensive literature search for the identification, retrieval, and bibliographic management of independent studies to locate information on the topic. A computerized search of the published literature of Korea(KISS, RISS), China(CNKI), Japan(Kampo medicine, etc), and western countries(MEDLINE) was performed, and further supplemented with manual searches of print sources(1999 to 2003). Results : The herbal medicines with angiogenic activity were mainly found among herbs that carry replenish Shin-Cheng(補腎益精), foster Eum and improve the circulation of blood(養陰活血), or warm and circulate Kyung-Rark(溫經通絡). In particular, herbs with improve the circulation of blood and clear blood(活血化瘀) activity contain a significant amount of tannin, saponin, and pyrazine. Conclusion : Replenish Ki-Hyul(補氣血) and circulate Kyung-Rark(通經絡) could contribute to the induction of angiogenesis because various growth factors and proliferation, differentiation, and migration of vascular endothelial cells are involved in angiogenic activity.

交藤圓이 白鼠의 酸化誘發을 防禦하는 作用에 關한 硏究

이송실(Song-Shil Lee),이상재(Sang-Jae Lee),김광호(Kwang-Ho Kim) 대한한의학회 2004 대한한의학회지 Vol.25 No.2

경옥고(瓊玉膏)가 노화유발 흰쥐의 항산화능에 미치는 영향

곽병준,이송실,백진웅,이상재,김광호,Kwak Byung-Jun,Lee Song-Shil,Baek Jin-Woong,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2003 대한예방한의학회지 Vol.7 No.2

Objectives : In order to examine the antioxidant activities of Kyungohkgo(瓊玉膏), the study was done through measurement of parameters such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px), Plasma total lipid, Plasma total triglyceride, Plasma total cholesterol, HDL-cholesterol concentrations in rat erythrocytes and plasma. Methods : Sprague-Dawley rats divided into 3 groups, Normal group(l2 weeks old), pathologically induced group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, CONTROL) and Kyungohkgo(瓊玉膏) administered group(D-galactose 50mg/kg and Kyungohkgo extracts 1125.0mg/kg 1time/day for 6 weeks, KOG). Rats were sacrificed and TBARS, SOD, CAT, Plasma total lipid, Plasma triglyceride, Plasma total cholesterol, Plasma HDL-cholesterol concentrations and GSH-px were measured in rat erythrocytes and plasma. Results : Plasma TBARS concentrations of KOG group were significantly lower than those of control. Red blood cell(RBC) SOD activities of KOG group was increased(F=3.619, p=0.052, ANOVA test), and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of KOG group was increased(F=6.844, p=0.008, ANOVA test). The changes of Plasma triglyceride was not significantly different. Plasma total lipid of KOG group showed significant decrease compared to the control group(F=19.337, p=0.0001, ANOVA test). Plasma total cholesterol and HDL-cholesterol concentrations of all experimental groups were not significantly different. Conclusions : According to the above results, it is considered that Kyungohkgo(瓊玉膏) is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

팔괘(八卦)로 해석(解釋)한 장부별(臟腑別) 정신양생법(精神養生法)에 관한(關) 연구(硏究)

백진웅,이송실,이상재,김광호,Baek Jin-Woong,Lee Song-Shil,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2004 대한예방한의학회지 Vol.8 No.1

The research method of 'the grafing I-ching(周易) and oriental medicine'(醫易會通) has been handed down for more than 2000 years to interpret the theory of the medicine by using the system of the ideology and the category of I-ching(周易). Many skillful doctors form the past have developed and advanced the oriental medicine by studying the 'the grafing I-ching(周易) and oriental medicine'(醫易會通) and wrote many theories and had experiment on clinical studies. From many theories of 'the grafing I-ching(周易) and oriental medicine'(醫易會通), I believe using the ideology and the category of eight trigrams(八卦) system to study the internal organs(臟腑) has a great importance. For eight trigrams(八卦) is the nucleus or the basic of I-ching(周易) and the internal organs (臟腑) are the main system of the body. Therefore grafting eight trigrams(八卦) and the internal organs (臟腑) theory has a meaning of grafting I-ching(周易) and the main point of the oriental medicine. But the uncertainties of the theories between doctors still exists and there's no unified theories in matching eight trigrams(八卦) and the internal organs(臟腑). This tells us that the most important part of the 'the grafing I-ching(周易) and oriental medicine'(醫易會通) theory is not logically organized. Therefore the author realized the necessity of the study about grafting eight trigrams(八卦) and the intimal organs (臟腑) theory, Also in order to confirm the significance of the result of the research, first of all there needs to be a research that applies it to the meathod of the mental cultivation(精神養生法), Health preservation(養生) is the basic idea of the oriental medicine and among health preservation(養生), the meathod of the mental cultivation(精神養生法) is most important.

복분자(覆盆子)가 노화유발(老化誘發) 흰쥐의 항산화능(抗酸化能)에 미치는 영향(影響)

박성준,이송실,백진웅,이상재,김광호,Park Sung-Jun,Lee Song-Sil,Baek Jin-Woong,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2004 대한예방한의학회지 Vol.8 No.1

Objectives: Rubi Fructus(fruit of Rubus coreanus Miq.) composed of Polygonum multiflorum THUNB. and some medical herbs is known as formula of senescence delay effect. The purpose of this study is to investigate the effect of Rubi Fructus(fruit of Rubus coreanus Miq.) on antioxidant enzyme activity such as Thiobarbituric acid reactive substance(TBARS), Superoxide dismutase(SOD), Catalase(CAT), Glutathione peroxidase(GSH-px) in rat erythrocytes and blood plasma. Methods: Sprague-Dawley rats were divided into 3 groups, Normal group(supplied enough water and feeds only, Normal Group), D-galatose administered group(injected D-galatose 50mg/kg, 1time/day for 6 weeks, Control Group) and Rubi Fructus (fruit of Rubus coreanus Miq.) administered group(D-galactose 50mg/kg and Rubi Fructus(fruit of Rubus coreanus Miq.) extracts 85.0mg/200g 1time/day for 6 weeks, BBJ Group). Rats were sacrificed and TBARS, SOD, CAT, GSH-px, Plasma total lipid, Plasma triglyceride and cholesterol were measured in rat erythrocytes and blood plasma. Results : Plasma TBARS concentrations of all experimental group were not significantly different. Red blood cell(RBC) SOD activities of BBJ group was increased, and RBC catalase activities of all experimental group were not significantly different. RBC GSH-px activities of BBJ group was increased. Plasma total lipid concentration of BBJ group were significantly lower than those of control. Plasma triglyceride, total cholesterol and HDL-cholesterol concentrations of all experimental group were not signi ficantly different. Conclusions: According to the above results, it is considered that Rubi Fructus(fruit of Rubus coreanus Miq.) is effective in inhibiting lipid peroxidation and increasing antioxidative enzyme activities in D-galactose induced aging rat.

명청시기(明淸時期) 소아추나(小兒推拿)에 관(關)한 문헌적(文獻的) 고찰(考察)

정국훈,이송실,백진웅,이상재,김광호,Ding Guo-Xun,Lee Song-Shil,Baek Jin-Woong,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2004 대한예방한의학회지 Vol.8 No.2

Bibliographic study on pediatric Chuna in the Myung Chung dynasties resulted as the following. 1. Pediatric Chuna experts in Myung dynasty utilized methods of Chuna to a great extense as means of preventing and treating various kinds of pediatric disorders. 2. Pediatric Chuna experts in Myung dynasty put emphasis on diagnosis through the doctor's inspection. 3. Pediatric Chuna experts in Myung dynasty developed and applied special acupoints for pediatric Chuna. 4. Pediatric Chuna experts in Myung dynasty put emphasis on replenishing and discharging according to hand manipulation. 5. Pediatric Chuna experts in Myung dynasty developed and applied methods of massaging with herbal medicine. 6. Pediatric Chuna experts in Myung dynasty put methods of Chuna into songs for handier use. 7. Pediatric Chuna experts in Chung dynasty corrected the errors of the predecessors and further developed the handed down advantages. 8. Pediatric Chuna experts in Chung dynasty specifically combined the theories of Chuna with characteristics of pediatric physiology. 9. Pediatric Chuna experts in Chung dynasty described treatments by symptoms systematically. 10. Pediatric Chuna experts in Chung dynasty, criticizing the trend at the time of despising pediatric Chuna, endeavored to spread pediatric Chuna. 11. Pediatric Chuna experts in Chung dynasty arranged the relationship between hand manipulation of Chuna and herbal medicine. 12. Pediatric Chuna experts in Chung dynasty arranged various kinds of methods for diagnosis, including diagnosis by palpation of the chest and the abdomen. 13. Pediatric Chuna experts in Chung dynasty combined the method of multiple hand manipulation and method of basic hand manipulation. 14. The theories of pediatric Chuna before Myung Chung dynasties only put emphasis on Ki and Blood, not combining with the basic principles of Korean medicine like Eum/Yang and Five Phases. The scholars of Myung Chung dynasties came to successfully combine the principles of Korean medicine like stability theory, Jang and Bu (internal organs) theory, and Eum-Yang theory with the theories of pediatric Chuna. This combination best characterizes the theories of pediatric Chuna in the Myung Chung dynasties.

산사(山査)가 식이성 고지혈증 흰쥐의 혈청지질에 미치는 영향

이승기,이송실,백진웅,이상재,김광호,Lee Sung-Ki,Lee Song-Shil,Baek Jin-Woong,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2003 대한예방한의학회지 Vol.7 No.2

Objectives: CRATAEGII FRUCTUS(山査) is known as the substance which decreases concentration of sarum lipid. The purpose of this study is to investigate the effects of CRATAEGII FRUCTUS(山査) on decreasing concentration of sarum lipid which are triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol in rat high cholesterol diet inducede hyperlipide rats. Methods: Sprague-Dawley rats were divided into 3 groups, Normal group(supplied enough water and feeds only. Normal Group), high fat diet administered group(AIN-76 high fat diet #100496 used beef tallow for 4 weeks, Control Group) and CRATAEGII FRUCTUS(山査) administered group(AIN-76 high fat diet #100496 used beef tallow and CRATAEGII FRUCTUS(山査) extracts 85.0mg/200g 1time/day for 4 weeks. SS Group). Rats were sacrificed and concentration of triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol were measured in rat's sarum lipid Results: There was no significant difference in the level of waight between each experimental group(F=0.243, p=0.787, ANOVA test), Concentration of triglyceride has no significant difference in each group(F=0.210, p=0.813, ANOVA test). There was significant increase of total cholesterol in the Control group compared to those of Normal but there was no significant difference in the SS group compared to the Control group(F=5.237, p=0.0l9, ANOVA test). There was a significant increase of HDL-cholesterol in the SS group compared to the Control group and decrease in Control group compared Normal group(F= 11.437, p=0.001, ANDYA test). Concentration of LDL -cholesterol was significantly different in each group(F= 16.377, p=0.0001, ANDYA test). It was significantly increased in the Control group compared with the Normal group and it has no difference in the SS group compared to the control group. TBARS values(Thiobarbituric Acid Reactive Substances) were significantly different in each group(F=5.242, p=0.0019, ANDYA test). It was significantly increased in the Control group compared with the Normal group and significantly decreased in SS group compared with the Control group. Conclusions: According to the above results, it is considered that CRATAEGII FRUCTUS(山査) is effective in decreaseing concentration of sarum lipid in high cholesterol diet induced hyperlipidemic rats.