http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
4배체 현사시나무 (Populus alba L. X P. gludulosa Uyeki)의 약배양에 의한 식물체 재분화
손성호,김정희,문흥규,노은운,이윤희,김미희,박진선,이용욱,윤양,이석구,Son, Sung-Ho,Kim, Jung-Hee,Moon, Heung-Kyu,No, Eun-Woon,Lee, Yoon-Hee,Kim, Mi-Hee,Park, Jin-Sun,Lee, Yong-Wook,Yoon, Yang,Lee, Seok-Gu 한국식물생명공학회 1995 식물생명공학회지 Vol.22 No.3
현사시나무 4배체의 약배양에 의하여 2배체 식물을 얻을 수 있었다. 약배양에 의한 캘러스 유도에는 2,4-D가 효과적 인 것으로 나타났다. 증식된 캘러스로부터 식물체 재분화를 위해서는 16시간의 광조건하에 6-8 $\mu$M의 zeatin 처리가 가장 좋은 반응을 보였는데 이때 얻어진 줄기수는 평균 7.7개 였다. 재분화된 식물체는 MS 기본배지에서 쉽게 뿌리를 유기시킬 수 있었으며, 이들 식물체는 폿트묘로 육성하여 온실 및 포지로 이식하였다. 약배양유래의 300클론에 대한 생육상태를 조사한 결과 삽목묘에 비하여 33%는 생장이 저해됨을, 47%는 생장이 더 좋은 것으로 나타났으며, 그중 20%는 삽목묘에 비해 직경이 5배 이상되는 거대 엽을 가지고 있었다. 염색체 수는 대체로 2배체성 혹은 4배성을 띠고 있었으며, RAPD marker를 이용하여 DNA polymorphism을 본 결과 약배양 유래의 몇몇 식물체에 있어 서 밴드 형태적인 차이를 나타내는 개체를 발견할 수 있었다. Diploid plants were obtained by anther culture of tetraploid poplar(Populus alba L. X P.glandutosa Uyeki). The effect 2,4D on callus formation from anther culture was greater than any other auxins tested. The highest average number of multiple shoots per callus was obtained when zeatin was used at levels of 6-8 ${\mu}$M. Regenerated shoots were excised and transferred to MS basal medium. Rooted plantlets were subsequently transferred to pots containing artificial soil mix. Finally 100 plane were transplanted in nursery located in forest Genetics Research Institute. for the 300 anther clones growing in greenhouse for 6 months after transplanting, 33% were slow-growing, 47% were rapid-growing and 20% had huge leaf size with rapid-growing characteristics. Chromosome study showed a narrow range of variation from diploid to tetraploid. DNA polymorphism studies using various RAPD markers revealed some extend of differences among the anther-clones in their band pattern.
참나무류 수형목 (秀型木) 37가계의 기내증식 (器內增殖)
문흥규(Heung Kyu Moon),윤양(Yang Youn),손성호(Sung Ho Son),이석구(Suk Koo Lee),이재선(Jae Sun Yi) 한국산림과학회 1993 한국산림과학회지 Vol.82 No.1
In vitro shoot proliferation and rooting were tested for 2-0 seedlings of half-sib families of 4 plus oaks trees. Nodal segments having axillary buds from 37 families(16 of Quercus acutissima, 10 of Q. variabilis, 7 of Q. serrata, and 4 of Q. mongolica) were cultured on WPM (Woody Plant Medium) supplemented with 0.5 ㎎/ℓ BA (6-benzyladenine) and 0.01 ㎎/ℓ NAA(α-naphthalene acetic acid) and subcultured at 2-3 weeks of intervals fur 6 months. In vitro rooting was carried out on GD(Gresshoff and Doy) medium supplemented with 0.5㎎/ℓ IBA(ndole butyric acid). The capacity for shoot proliferation and rooting was highly varied with families. Generally, white oaks(Q.serrata and Q. mongolica) showed poor response than black oaks(Q. acutissima and Q, variabilis) in shoot proliferation and rooting. Among the total of 37 families, 7 of Q. acutissima, each 2 of Q. variabilis, Q. serrata, and Q. mongolica revealed abilities for continuous shoot proliferation, and the others failed to proliferate. Rooting of the selected oak trees also greatly varied among the families. In Q. acutissima, rooting ratio ranged from 10.0% (CB 25. KG 4) to 89.8% (CB 18). Although 26.7% of KG 16 in Q. variabilis, 3.3% of JN 15 in Q. serrata were rooted, Q. mongolica was not rooted at all in this experimental conditions. No relationship between shoot growth and the rooting ability was observed. Present results suggest the possibility of large-scale micropropagation, but further studies on family differences, shoot-tip necrosis, and callusing of rooting junction are still required to develop reliable micropropagation systems.
이상선,백기엽,가강현,이석구 한국균학회 1991 韓國菌學會誌 Vol.19 No.3
Out of the 36 species (22 families) of horticultural plants collected from the horticultural shop around Cheong Ju, the 17 plant species (47.2%) were infected with VA-mycorrhizae in the root tissues. Also, the chlamydospores or azygospores of VA-mycorrhizae were identified (two genera, three species); Acaulospora spinosa, Glomus etinucatum, and G. tortusom. VA-mycorhizae found from the cultivated plants around Korea National University of Education and other area were also identified (four genera, six species); A. myriocarpa, Gigaspora decipiens, G. caledonium, G. glomerulatum, G. microcarpum, and Scutellospora calospora.
문옥륜,장동민,이석구,강선희,박실비아,최상은 서울大學校保健大學院 1995 國民保健硏究所硏究論叢 Vol.5 No.2
Since the introduction of the National Health Insurance Program as of 1977, the charges for medical treatment have been increased as much as by 15 times. In general, Fee schedules should be decided upon by taking account of the ability to pay for medical services by consumers, and the financial status of both insurers and medical facilities. As the policy makers have mainly concerned about controlling the price of medical service, the level of fee schedules has kept low: the accumulative index of the charge for medical treatment showed only 89% as against the accumulative consumer's price index during the last 18 years. This lower increasing rate has caused us many undesirable outcomes. For example, medical practices have been twisted to orient toward higher earning practices and high cost equipments have been introduced imprudently. This study is to develop a new method of determining the adequate increasing rate for medical fee schedules. The data complied by KIHM were used such as 'Analysis of the Hospital Management, 1992' and the 'study on the Adjusted Increasing Rate for the Insurance Fee Schedules, 1994'. The basic model of updating fee schedules have been developed to include deficit rates and the incentive for medical service quality improvement, and the final adjustment rate was derived from it accordingly. If the authorities decide to increase fee schedules as of January 1995, the adjustment by 12.59-14.63% will be enough and if it becomes June 1995, the rates will go up as much as by 21.58-25.08%. Furthermore, this study shows that the government should regularize the period of increasing time and this will make medical facilities to predict their operational budgets possible.