레드 비트 뿌리 추출물의 항산화 및 항염증 효과

이미란,강창희,부희정,Yi, Mi-Ran,Kang, Chang-Hee,Bu, Hee-Jung 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.3

본 연구는 제주도에서 재배된 레드 비트(B. vulagaris) 뿌리의 항산화 및 항염 효과를 알아보기 위하여 70% EtOH 추출물과 순차적 용매 분획물들을 확보하여 총폴리페놀 함량 및 ABTS 라디칼 소거 활성 측정을 통한 항산화 효능평가와 대식세포인 RAW264.7 세포에 LPS를 자극한 후 iNOS/NO, $COX-2/PGE_2$ 및 전염증성 cytokine을 유도하여 염증 억제 효과를 알아보았다. 그 결과, 총 폴리페놀이 37.02 mg GAE/g로 가장 높게 나온 EtOAc 분획물이 라디칼 소거활성도 가장 우수하게 나타났으며($IC_{50}$ $42.9{\mu}g/mL$), 대조군으로 사용한 BHT($IC_{50}$ $57.4{\mu}g/mL$)보다 좋은 활성을 보였다. 항염 활성 분석을 위하여 NO/iNOS, $PGE_2/COX-2$, 및 염증성 cytokine($TNF-{\alpha}$, IL-6, $IL-1{\beta}$)의 생성 억제 효능을 분석한 결과, 총 폴리페놀 함량 결과와는 유의적이지 않게 hexane 분획물이 처리 농도 범위(50, 100, 200, $300{\mu}g/mL$)에서 NO/iNOS, $PGE_2/COX-2$, 및 염증성 cytokine 생성을 유의적으로 억제하였다. iNOS와 COX-2 단백질 발현 억제 효과를 통해 NO와 $PGE_2$ 생성 억제에 영향을 끼치고 있음을 확인하였고, 염증성 cytokine 중에는 IL-6의 생성을 가장 강하게 억제함으로써 전체적으로 항염 활성에 영향을 미치고 있음을 확인할 수 있었다. 이러한 결과들로부터 레드비트 뿌리의 EtOAc 분획물에서의 항산화 효능 확인과 hexane 분획물의 세포내 항염 효과를 알 수 있었으며, 향후 유효 물질 동정을 통한 기전 연구를 하는 데 대한 기초자료로 활용할 수 있을 것이라 사료된다. This study was designed to examine the in vitro antioxidant and anti-inflammatory effects of red beet (Beta vulagaris) root. Red beet root was extracted using 70% ethanol and then fractionated sequentially with n-hexane, ethyl acetate and butanol. Antioxidative ability was evaluated by bioassays using total polyphenol contents and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid diammonium salt) radical scavenging activity. Ethyl acetate fraction of red beet root was best on total polyphenol contents ($37.02{\pm}0.37mg\;GAE/g$) and ABTS radical scavenging effects ($IC_{50}$ $42.9{\pm}9.5{\mu}g/mL$). For the anti-inflammatory activity in RAW264.7 cells, the hexane fraction showed the highest inflammatory effect. Dose response studies were performed to determine the inhibitory effect of hexane fraction of red beet root on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The hexane fraction of red beet root inhibited the NO and $PGE_2$ production and the protein level of iNOS and COX-2, and protein expression of pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6 and $IL-1{\beta}$), in a dose-dependent manner. These results suggest that red beet root has considerable potential as a functional food ingredient with antioxidative and anti-inflammatory effects.

레몬그라스 식초의 초산발효 특성과 항산화 활성

이미란,강창희,부희정,Yi, Mi-Ran,Kang, Chang-Hee,Bu, Hee-Jung 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.5

본 연구는 제주도에서 재배된 레몬그라스를 활용한 식초의 초산발효 특성과 품질특성 그리고 항산활 활성을 알아보았다. 레몬그라스를 고두밥대비 5% 첨가한 현미주정을 제조한 결과 알코올 함량이 7.2%였다. 여기에 초산균인 Acetobacter sp. RIC-V 종초를 10% 가하여 초산발효하여 레몬그라스 식초를 제조하였다. 초산발효 결과 pH가 3.62에서 3.13으로 낮아졌으며, 총산도는 1.43%에서 7.21%로 증가하였다. 레몬그라스 식초의 유리당 분석결과 fructose가 0.81 g/100 g으로 나타났고, glucose, sucrose, maltose는 검출되지 않았다. 유기산 분석결과는 acetic acid가 3,658.58 mg%로 가장 높았으며, citric acid, lactic acid가 각각 214.66 mg%, 159.44 mg%로 나타났고, malic aicd, tartaric acid, oxalic acid 모두 소량 함유한 것으로 분석되었다. 유리아미노산 분석결과 glutamic acid가 0.233 g/100 g으로 가장 높은 함량을 보였고, histidine, alanine, proline, aspartic acid이 각각 0.129 g/100 g, 0.113 g/100 g, 0.103 g/100 g, 0.069 g/100 g으로 나타났다. 총 폴리페놀 함량 분석 결과는 레몬그라스 식초와 레몬그라스 무첨가 식초가 각각 $568.6{\mu}g\;GAE/mL$, $490.9{\mu}g\;GAE/mL$이며, 레몬그라스 식초에 레몬그라스 분말과 추출물의 첨가량이 높을수록 높아져 레몬그라스 분말 5% 첨가군이 $895.7{\mu}g\;GAE/mL$로 가장 높았다. ABTS 라디칼 소거활성은 레몬그라스 식초와 레몬그라스 무첨가 식초 2.5%농도에서 각각 58.0 43.2%로 분석되었으며, 이 또한 레몬그라스 분말과 추출물의 첨가량이 높을수록 높아져 레몬그라스 분말 5% 첨가군이 91.0%로 가장 높은 저해율을 보였다. 결과 레몬그라스 식초는 총산도 7.21로 높고, 유기산, 아미노산 함량이 우수하며, 항산화 활성이 우수한 것으로 나타나 고품질의 기능성 식초로 활용 가능할 것으로 사료된다. This study investigated acetic acid fermentation properties and antioxidant activity of vinegar by addition of lemon grass to develop high quality vinegar by using lemongreass. Traditional brown rice wine contained 5% lemongrass powder and had an alcohol content of 7.2%. The wine was fermented by Acetobacter. sp. RIC-V and made into lemongrass vinegar (LV). The pH and total acidity of the LV were 3.13% and 7.21%, respectively. Fructose was detected whereas glucose, sucrose, and maltose were not detected. Among organic acids, acetic acid was highest at 3658.6 mg%; trace amounts of lactic acid, citric acid, malic acid, tartaric acid, and oxalic aicd were detected. Of the 17 free amino acids, glutamic acid, histidine, alanine, and proline were mainly detected. To conduct total polyphenol content and ABTS radical scavenging activity, 3% and 5% lemongrass powder (P3LV, P5LV) and 1%, 2%, and 3% of lemongrass extract (E1LV, E2LV, E3LV) were added to LV, respectively. Total phenolics increased as the added lemongrass powder and extract increased. Total phenolics were 490.9, 559.4, and $895.7{\mu}g$ gallic acid equivalents/mL in brown rice vinegar, LV, P5LV. ABTS radical scavenging activities were 43.2%, 58.0%, and 91.0% in brown rice vinegar, LV, P5LV, respectively. These results show that lemongrass vinegar has considerable potential as a high quality functional vinegar with antioxidative effects.

35종 에센셜 오일의 항산화, 항균 및 멜라닌 합성 저해 효과

이미란 ( Mi-ran Yi ),부희정 ( Hee-jung Bu ) 한국미용학회 2017 한국미용학회지 Vol.23 No.4

This study was designed to examine the antioxidant, whitening and antimicrobial effects of 35 species essential oils. Antioxidative ability was evaluated by bioassay using total polyphenol contents, 2,2`-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2, 2- diphenyl-1-1-picrydrazyl (DPPH) free radical scavenging activity. Ocimum basilicum (basil) and cymbopogon citratus (lemongrass) essential oils showed excellent total polyphenol contents 1831.8, 1202.0 μg gallic acid equivalents/mL, repectively. While, radical scavenging activity were exhibited basil and vetiveria zizanoides (vetiver) essential oils powerfully; ABTS radical scavenging activities of both basil essential oil and vetiver essential oil were 98.2±0.7% and 99.4±0.9%. DPPH free radical scavenging activities of the two oils were 94.2±0.1% and 94.5±0.6% respectively, at a concentration of 1%. The antimicrobial activity of essential oil against skin pathogens was determined by paper disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Essential oils of labiatae, graminaeae, annonaceae and lauraceae were showed nice antimicrobial activities against strain of two and over among Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Propionibacterium acnes (P. acne). Especially, essential oils of graminaeae demonstrated good antimicrobial effects against P. acne. For the study of whitening activity in B16F10 melanoma cells, basil, citrus aurantium (petitgrain), cymbopogon nardus (citronella), eucalyptus radiata, pinus sylvestris (pine), pelargonium graveolens (geranium rose) oils inhibited significantly melanin contents 50.5±2.2, 65.8±2.74, 76.4±0.8, 68.1±2.2, 74.5±1.9, 79.5±4.1% respectively, at concentration of 0.04% without cytotoxicity. These results suggested that antimicrobial effect of essential oils against P. acne may have not only similar characteristic among plants in the same family, but also the considerable potential as a various skin care ingredient with antioxidative, antimicrobial and whitening effects.

콜라비 추출물의 항산화 및 항염 효능

이미란(Mi Ran Yi),강창희(Kang Chang Hee),부희정(Hee Jung Bu) 한국유화학회 2017 한국응용과학기술학회지 Vol.34 No.2

본연구는 콜라비 추출물의 항산화 및 항염 효능을 조사하기 위하여 수행하였다. 콜라비는 70% 에탄올을 이용하여 조추출한 후 헥산, 에틸아세테이트, 부탄올을 이용하여 용매 극성에 따라 순차적으로 분획하였다. 항산화 활성은 총폴리페놀 함량 측정과 ABTS 라디칼 소거활성을 측정하여 평가하였다. 에틸아세테이트 분획물이 총폴리페놀 함량(27.33±0.26 mg GAE/g)과 ABTS 라디칼 소거활성(IC50 172.9±1.6 ㎍/mL)이 가장 높게 측정되었다. 항염 활성은 RAW 264.7 세포를 이용하였으며, 에틸아세테이트 분획물이 가장 높은 항염 활성을 보였다. 에틸아세테이트 분획물의 LPS로 유도된 RAW 264.7 세포에서 전염증성 매개인자들에 대한 저해효과를 농도별로 측정하여 확인하였다. 콜라비 에틸아세테이트 분획물은 NO, PGE₂ 생성과 iNOS와 COX-2 및 TNF-α, IL-6, IL-1β와 같은 전염증성 사이토카인들의 단백질 발현을 농도의존적으로 저해하였다. 이러한 결과들은 콜라비가 항산화 및 항염 효능을 가지는 소재로서의 개발 가능성이 있음을 시사한다. This study was designed to examine the in vitro antioxidant and anti-inflammatory effects of kohlrabi (Brassica oleracea var. gonglodes) extract. Kohlrabi was extracted using 70% ethanol and then fractionated sequentially with n-hexane, ethyl acetate and butanol. Antioxidative ability was evaluated by bioassays using total polyphenol contents and ABTS (2,2 -azino-bis (3-ethylbenzothiazoline-6- sulphonic acid diammonium salt) radical scavenging activity. Ethyl acetate fraction of kohlrabi was best on total polyphenol contents (27.33±0.26 mg GAE/g) and ABTS radical scavenging effects (IC50 172.9±1.6 ㎍/mL).For the anti-inflammatory activity in RAW 264.7 cells, the EtOAc fraction showed the highest inflammatory effect. Dose response studies were performed to determine the inhibitory effect of EtOAc fraction of kohlrabi on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The EtOAc fraction of kohlrabi inhibited the NO and PGE₂ production and the protein level of iNOS and COX-2, and protein expression of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β), in a dose-dependent manner. These results suggest that kohlrabi has considerable potential as a ingredient with antioxidative and anti- inflammatory effects.

감귤 미숙과 식초의 품질 특성과 항산화 활성

이미란(Mi-Ran Yi),황준호(Joon-Ho Hwang),오유성(You-Sung Oh),오현정(Hyun-Jeong Oh),임상빈(Sang-Bin Lim) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.2

폐자원인 감귤 미숙과를 이용한 식초를 개발하기 위하여 초산 생성능이 우수한 균주를 분리 동정하였고, 감귤 미숙과를 발효하여 식초를 제조한 후 품질 특성, 총 페놀 및 총 플라보노이드 함량, 항산화 활성을 측정하였다. 감귤슬러지 자연발효물로부터 5개 균주를 분리하였으며, 그중 초산균으로 Acetobacter fabarum(A. sp. RIC I)과 A. pomorum(A. sp. RIC II, V)이 동정되었는데, A. sp. RIC V가 초산 생성능이 가장 우수하여 감귤 미숙과즙의 초산발효 균주로 선정하였다. A. sp. RIC V를 이용하여 감귤 미숙과즙의 첨가비율을 30, 35, 40%로 달리하였을 때 발효 13일 후 산도는 각각 5.38, 5.38, 5.32%, 발효 효율은 각각 73, 72, 70%이었다. 미숙과즙 초산발효 시 산도가 5%에 도달하는 기간은 과즙첨가비율이 30%일 때는 11일, 35와 40%일 때는 9일이었다. 유리당으로 fructose와 glucose는 과즙 첨가비율이 높을수록 높은 함량을 나타내었으나 sucrose는 과즙 첨가비율과 관계없이 모두 비슷하였다. 유기산의 경우에는 미숙과 과즙의 첨가비율이 높을수록 acetic acid를 비롯한 총 유기산이 증가하였다. 감귤 미숙과 식초와 완숙과 식초의 총 페놀 함량은 각각 1,546.6과 230.9 μg GAE/mL이었고, 총 플라보노이드 함량은 각각 1,004.7과 175.1 μg QE/mL이었다. DPPH 자유라디칼 소거활성은 감귤 미숙과와 완숙과 식초가 각각 29%와 5%이었고, ABTS 소거능은 각각 62.0%와 17.9%였다. SOA 소거활성은 감귤 미숙과 식초와 완숙과 식초가 각각 60.9%와 41.7%이었고, XO 억제활성은 각각 32.5%와 5%이었다. 이상의 결과로부터 A. sp. RIC V를 이용하여 감귤 미숙과즙을 35와 40% 첨가하였을 때 초산발효가 가장 우수하였으며, 감귤 미숙과 식초는 높은 총 페놀과 총 플라보노이드 함량으로 인하여 높은 항산화 활성을 나타내어 기능성 식초로 활용할 수 있을 것이다. To develop vinegar with immature Citrus unshiu (IC), bacterial strains with high acetic acid-producing capabilities were isolated and identified, after which their quality characteristics, total phenolic and total flavonoid contents, and antioxidant activities were measured. Five bacterial strains were isolated from naturally fermented C. unshiu, and three were identified as Acetobacter fabarum (A. sp. RIC I) and A. pomorum (A. sp. RIC II, V). A. sp. RIC V showed the highest acetic acid-producing capability and was thus chosen as the candidate strain for further acetic fermentation using IC juice. Vinegars made with 30, 35, and 40% IC juices showed acidities of 5.38, 5.38, and 5.32% and fermentation efficiencies of 73, 72, and 70%, respectively. The fermentation periods required to reach greater than 5% acidity were 11, 9, and 9 days for vinegars containing 30, 35, and 40% IC juices, respectively. Fructose and glucose contents of the vinegars increased along with total organic acid contents including acetic acid, with increasing IC juice contents. Total phenolics were 1,546.6 and 230.9 μg GAE/mL, whereas total flavonoids were 1,004.7 and 175.1 μg QE/mL in vinegars made with IC and mature C. unshiu (MC) juices, respectively. DPPH free radical scavenging activities were 29% and 5%, ABTS radical scavenging activities were 62.0% and 17.9%, SOA scavenging activities were 60.9% and 41.7%, and XO scavenging activities were 32.5% and 5% in vinegars made with IC and MC juices, respectively. Therefore, vinegars made with 35% and 40% IC juices using A. sp. RIC V as the acetic acid fermentation strain showed potent antioxidant activities with greater total phenolic and flavonoid contents, promoting their use as functional vinegars.

기하학적 형태표현에 관한 연구

이미란(Mi Ran Yi) 한국일러스아트학회 2004 일러스트레이션학 연구 Vol.15 No.-

Through the stream of history, it was realized that there was limit in expressidng the complexity and diversity of geometrical form. Geometrical form is used to describe shapes as cognized as attempted scientific method which combines space artand create form. It has surely changed our notion of aesthetics to review the harmony, suggesting a new direction to the complicated form of geometric. The purpose of this study is to propose a new design pnnaples and to analyze the Geometrical form and applying Gecniethcal method. As this study is based on geometry as a natural science, I infected to histon method and provide some methods of generating properties.

SI작업의 일환으로 판매환경 집기디자인에 관한 연구 - A 브랜드를 중심으로 -

이미란(Mi Ran Yi),권석준(Suk Jun Kwon),박우장(Woo Jang Park) 한국가구학회 1999 한국가구학회지 Vol.10 No.1

This study aims to organize the research ways of interior design for clothing stores as a management strategy in the market changing rapidly. The public met their needs in a mass production system. However, the brand-name companies began to create the conditions of consumption for people, there appeared people with new lifestyles and new way of thoughts so that their propensity to consume resulted in consumption activities, and it became varied, individual, and highly qualified.<br/> Therefore, in view of consumers' priority, based on 51 sales environment this paper aims to create a new image by re-designing the interior and furniture of shops which will help to enhance the image of brand name. Therefore, the brand-name companies will expect a large sale for the new product and it will apply to sales environments of other products and influence them. The purpose of this paper is to find a desirable way to increase sales efficiency for the companies.<br/> In order to get a concept and propriety of sales environment design. examined mutual relation to each study of interior design, marketing, and fashion design.<br/> Through referring to the documents research of these three fields I could get a conception and basic structure for them. Based on the theoretical knowledge, this study was made to show to examine and generalize the existing sales environments and situations and set up a new concept for this. It will apply to interior design and store holds and present a new design alternative for improvement.<br/>

인동꽃 에센셜 오일의 항산화 및 항염 활성

전아림 ( Ah-lim Jeon ),이미란 ( Mi-ran Yi ),강창희 ( Chang-hee Kang ),부희정 ( Hee-jung Bu ) 대한미용학회(구 대한미용과학회) 2016 대한미용학회지 Vol.12 No.4

This study examined the antioxidant and anti-inflammatory effects of essential oil from the flowers of Lanicera japonica in order to verify whether the oil can be used as a cosmetic ingredient. Antioxidative capacity was evaluated by bioassay using total polyphenol and flavonoid contents as well as 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. Total polyphenol and flavonoid contents were 31.8±1.1 mg GAE/g and 42.0±0.6 mg QE/g, respectively. ABTS radical scavenging activity was 84.6±0.3% and DPPH free radical scavenging activity was 42.2±1.3% (at a concentration of 500 μg/mL). The antimicrobial activity of essential oil from the flowers of L. japonica against skin pathogens was determined using the paper disc diffusion method, the minimum inhibitory concentration, and the minimum bactericidal concentration values. Essential oil from L. japonica flowers showed antimicrobial activities against Staphylococcus aureus and Propionibacterium acnes. For the study of anti-inflammatory activity in RAW 264.7 cell, essential oil from L. japonica flowers inhibited not only nitrogen oxide production but also the expression of pre-inflammatory cytokines such as, tumor necrosis factor-α, interleukin (IL)-6 and IL-1β in a dose-dependent manner. Especially, at a concentration of 400 μg/mL, essential oil from L. japonica flowers strongly inhibited the cytokines production by 96.4±0.2, 72.6±4.5, 99.1±5.7, 97.6±2.6%, respectively. These results suggests that essential oil from L. japonica flowers has considerable potential as a cosmetic ingredient with antioxidative and anti-inflammation effects.

손바닥 선인장 엽상경의 품질 특성과 항산화 효과

황준호(Joon-Ho Hwang),이미란(Mi-Ran Yi),김재원(Jae-Won Kim),부희정(Hee-Jung Bu),강창희(Chang-Hee Kang),임상빈(Sang-Bin Lim) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.3

제주산 손바닥 선인장의 엽상경을 열수와 70% 에탄올로 추출한 후 분획하여 총 페놀 및 총 플라보노이드 함량과 항산화 활성 및 항염증 활성을 측정하였다. 총 페놀 함량은 에틸아세테이트 분획물이 784 mg GAE/g으로 가장 높았고 그 다음으로 부탄올 분획물(452), 헥산 분획물(220) 순이었다. 총 플라보노이드 함량도 에틸아세테이트 분획물이 214 mg GE/g으로 가장 높았고 헥산 분획물(113), 부탄올 분획물(76) 순이었다. 에틸아세테이트 분획물, 부탄올 분획물, 헥산 분획물의 DPPH 라디칼 소거능(IC50)은 각각 103, 359, 469 μg/mL였고, ABTS 라디칼 소거능(IC50)도 각각 105, 379, 605 μg/mL로 에틸아세테이트 분획물이 가장 높았다. 유해산소 억제 능력(ORAC)은 에틸아세테이트 분획물이 391 μM TE로 가장 높은 활성을 보였고 부탄올 분획물(117), 헥산 분획물(64) 순이었지만, superoxide anion 소거 활성(IC50, μg/mL)은 에틸아세테이트 분획물(40), 부탄올 분획물(69), 70% 에탄올(98) 순이었다. RAW264.7 세포에 의한 NO 생성 저해능(IC50, μg/mL)은 헥산 분획물(62), 에틸아세테이트 분획물(104), 부탄올 분획물(465) 순으로 높은 활성을 보였다. 세포독성 대비 NO 생성 저해 활성을 나타내는 SI 지수는 에틸아세테이트 분획물이 4.63으로 가장 높았고, 다음으로는 헥산 분획물(3.37), 부탄올 분획물(2.14), 증류수 추출물(1.66) 순으로, 양성 대조군으로 사용한 quercetin(6.25)보다는 낮았다. 결론적으로 제주산 손바닥 선인장 엽상경의 추출물과 분획물 중에서 에틸아세테이트 분획물이 항산화 활성과 항염 활성이 가장 높았으며, 이는 총 페놀과 총 플라보노이드 함량에 기인하는 것으로 추정되었다. Prickly pear cactus cladodes were extracted with hot water and 70% ethanol, followed by fractionation with n-hexane (HF), ethyl acetate (EF), n-butanol (BF), and distilled water. Total phenolics and total flavonoid contents as well as antioxidative and anti-inflammatory activities were then measured. Total phenolic contents were 784, 452, and 220 mg gallic acid equivalents (GAE)/g, whereas total flavonoid contents were 214, 76, and 113 mg quercetin equivalents (QE)/g in EF, BF, and HF, respectively. DPPH and ABTS radical scavenging activities (IC50) were 103 and 105 μg/mL in EF, 359 and 379 μg/mL in BF, and 469 and 605 μg/mL in HF, respectively. Oxygen radical absorbance capacity was highest at 391 μM TE in EF (in decreasing order of 117 μM TE in BF and 64 μM TE in HF), whereas superoxide anion radical scavenging activity (IC50) was highest at 40 μg/mL in EF (in decreasing order of 69 μg/mL in BF and 98 μg/mL in 70% ethanol extract). Inhibitory activity (IC50) of nitric oxide (NO) production induced by LPS-activated RAW264.7 cells was highest at 62 μg/mL in HF (in decreasing order of 104 μg/mL in EF and 465 μg/mL in BF). The selectivity index (ratio of inhibitory activity of NO production to cell cytotoxicity) was highest at 4.63 in EF (in decreasing order of 3.37 in HF and 2.14 in BF). In conclusion, EF showed potent antioxidant and anti-inflammatory effects with high phenolic and flavonoid contents.

제주산 로즈마리 에센셜 오일의 항염 및 피부 상재균에 대한 항균 활성

김소희(So-Hee Kim),이미란(Mi-Ran Yi),김창수(Chang-Soo Kim),김정미(Jung-Mi Kim),부희정(Hee-Jung Bu) 한국유화학회 2018 한국응용과학기술학회지 Vol.35 No.3

본 연구는 제주산 자생 로즈마리 에센셜 오일의 항염 및 피부상재균에 대한 항균 활성을 확인하기 위한 것이다. 로즈마리 에센셜 오일은 물 증류법으로 추출하였다. 로즈마리 에센셜 오일의 항염 효능을 확인하기 위하여 RAW 264.7 세포에서 LPS에 의해 유도된 NO와 PGE 2 의 생성을 농도 의존적 으로 억제하는 것을 확인하였다. Western blot 실험을 통해 이들을 생합성하는 효소인 iNOS, COX-2 단백질의 발현이 농도 의존적으로 감소하는 것을 확인하였다. 또한 전염증성 cytokine인 TNF-⍺와 IL-6의 생성 억제 효능을 확인하였다. 항생제 내성균주 각 2종을 포함한 S. epidermidis 3종, P. acnes 3종에 대한 항균 활성을 실험한 결과, paper disc법에서 저해환이 관찰되었고, MIC, MBC 실험을 통하여 균생육 저해와 사멸를 확인하였다. 위의 실험 결과로부터 로즈마리 에센셜 오일의 항염증, 항균 효능을 확인 하였으며 향후 화장품 및 스킨케어 소재로서 활용 가능성을 확인하였다. The purpose of this study was to investigate the anti-inflammatory effect and the antimicrobial activity to skin flora of essential oil from rosemary that naturally grown in Jeju. rosemary essential oil was extracted by water distillation essential oil extraction method. In order to confirm the anti-inflammatory activity of rosemary essential oil, it was confirmed that the production of NO and PGE 2 induced by LPS in RAW 264.7 cells was inhibited in a concentration-dependent manner. Western blot analysis showed that the expression of iNOS and COX-2, which are biosynthetic enzymes, decreased in a concentration-dependent manner. In addition, production of TNF-⍺ and IL-6 the pro-inflammatory cytokines were inhibited. Antimicrobial activities of three S. epidermidis and three P. acnes strains including two antibiotic resistant strains were observed in paper disc method and MIC and MBC tests showed inhibition of bacterial growth and death. From the results of the experiment, we confirmed that rosemary essential oil has the anti–inflammatory and antimicrobial efficacy and it could be used as a cosmetic and skin care material in the future.