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이동규,박제호,정인수,김태만,윤정현,Lee, Dong-Gyu,Park, Jae-Ho,Jung, In-Su,Kim, Tae-Man,Yoon, Jeong-Hyun 한국방사성폐기물학회 2011 방사성폐기물학회지 Vol.9 No.3
사용후핵연료 운반용기 표면온도가 $85^{\circ}C$를 초과할 경우, 대인용 보호막(Personnel Barrier) 또는 운반용 덮개(Transport Hood)를 설치하여 운반 중 운반용기 표면에 사람이 직접 접근할 수 없도록 하여야 한다. 운반용 덮개가 설치된 경우, 열적 안전성 평가의 한 가지 경우인 정상조건 열해석 시, 외부환경 경계조건(환경온도 및 외부복사온도)으로 적용하기 위해서 운반용 덮개 내부 열 환경 조건(내부 공기온도 및 운반용 덮개 표면온도)을 계산해야 한다. 따라서 본 연구에서는 운반용 덮개 내부 공기온도 및 표면온도를 계산하기 위한 해석적 방법 및 열전달 특성에 대한 분석을 수행하였고 CFD 해석 결과와 비교를 통해 타당성을 검증하였다. In case that the maximum temperature of any surface readily accessible during transport of a spent nuclear fuel (SNF) transport cask exceeds $85^{\circ}C$ in the absence of insolation under the ambient temperature of $38^{\circ}C$, personnel barriers or transport hood shall be used to prevent people from casual contact with the transport cask surface. Usually the air temperature within the hood and the hood surface temperature are calculated and further utilized as boundary conditions(free stream temperature and external radiation temperature) for thermal evaluation under normal conditions of transport. In this study, these temperatures are derived using the analytical method based on the heat transfer mechanism around the transport cask under transport hood assuming the thermal equilibrium. By comparing the analytical solutions with the results from the detailed calculations with CFD-computer-code FLUENT 12.1 it is verified that the analytical method is still efficient tool to estimate the temperatures and these temperatures can be further used as boundary conditions for thermal evaluation under normal conditions of transport.
pBRG-4 를 이용한 Metarhizium anisopliae 의 형질전환
이동규,예완해,황철원,권석태,강선철 ( Dong Gyu Lee,Wan Hae Yeh,Cher Won Hwang,Suk Tae Kwon,Sun Chul Kang ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.3
We have established a transformation system for entomopathogenic fungus, Metarhizium anisopliae, in order to develop mycoinsecticide by recombinant DNA techniques. Protoplasts of M. anisopliae would be transformed to a benomyl-resistant by introducing pBRG-4 plasmid DNA, which contains a β-tubulin gene of Aspergillus flavus conferring resistance to benomyl and a pyr4 gene of Neurospora crassa, in the presence of 5% polyethylene glycol and 10 mM calcium chloride. Transformants occuring at a frequency of 10 colonies per 50 ㎍ pBRG-4 DNA grew on the 5 ㎍/㎖ concentrations, of benamyl, while the wild type was inhibited by 2.5 ㎍/㎖. From the Southern analysis using genomic DNAs isolated from M. anisopliae transformants, the positive signals suggested that the β-tubulin gene had integrated in the M. anisopliae genome by homologous recombination.