우리나라에 서식하고 있는 모기 ( Culex pipiens )의 항원 추출과 항원성 분석

이기영,이기선(Ki Sun Lee),이수영(Soo Young Lee),정병주,김규언,류정우(Jeong Woo Ryu),염혜영(Hae Yung Yun),이한일(Han Il Lee),용태순(Tai Soon Yong),전성후(Soung Hoo Jeon) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 1999 소아알레르기 및 호흡기학회지 Vol.9 No.3

목 적 : 모기에 물린 후 나타나는 여러 가지 반응들은 혈청내 모기 특이 IgE 항체와 관련이 있는 것으로 알려져 있다. 이미 서구에서는 모기에 물린 후 팽진, 구진 등의 피부 국소반응과 출혈성 피부 병변, 혈관 부종, 천식 및 쇼크에 이르는 전신반응에 대한 증례 보고와 면역학적 연구가 시행되어 왔으나, 국내에서는 이에 관한 연구가 전무한 실정이다. 모기 항원은 모기의 몸체, 두흉부, 침샘, 침 등을 이용한 immunoblot 분석을 통해 알 수 있으며, 모기의 종에 따라 그 항원성은 상이하다. 정제된 항원의 제조는 모기 알레르기 환자의 진단 및 면역치료에 필수적이나, 상품으로 시판되는 모기 항원들은 현재까지 그 항원 농도와 단백질 농도가 일정치 않아 표준화가 필요한 것으로 판명되었다 이에 우리나라에서 서식하는 Culex pipiens의 항원을 추출하여 그 항원성을 분석해 보았다. 방 법 : 연세의대 소아과, 아주의대 소아과 알레르기 클리닉에 내원하여 시행한 피부 반응검사상 모기 항원에 양성 반응을 보인 환아들을 대상으로 하였다. 국립보건원 의동물학과로부터 사육중인 Culex pipiens를 공급받아 마취하에 두흉부를 분리하여 homogenizer를 이용하여 수용성 균질액을 만들고, 원심분리하여 filter에 투과시켜 조항원을 만들었다. Bradford method에 따라 단백질 농도를 측정하고, SDS-PAGE를 시행하여 분리한 후, nitroucellulose membrane에 전이시킨 후, 모기 항원으로 시행한 피부반응검사상 양성 반응을 보인 환아의 혈청을 이용하여 immunoblot을 실시하여 IgE와 결합하는 단백띠를 관찰하였다. 결 과 : 1) Culex pipiens 조항원의 단백질 농도는 10.88 mg/ml 이었다. 2) Culex pipiens 조항원의 SDS-PAGE 결과 20여개의 단백띠가 분리되었다. 3) Immunoblot 결과 70 kD, 60 kD, 34 kB의 단백띠가 IgE와 결합하였다. 결 론 ; 우리 나라에서 서식하는 모기 중 Culex pipiens의 조항원을 제조하였으며, 70 kD, 60 kD, 34 kD의 단백띠가 알레르기를 유발하는 항원으로 추측되며, 향후, 침샘 및 침을 이용한 정제된 항원을 이용한 연구가 시행되어야 할 것으로 기대된다. Purpose : Adverse reactions to mosquito bites have been recognized for some time, and these usually consist of large swellings, generalized urticaria, angioedema, and less frequently asthma. There have been some efforts to define immunologic characteristics of mosquito allergens throughout the world. But, in Korea, the immunologic analysis of mosquito allergen have not been made. Methods : Extracts of locally distributed female mosquito Culex pipiens, were prepared from their heads and thoraxes. By sodium dodecylsulfate-polyacrylamide gel electrophoresis, proteins of two extracts were isolated. Then their proein bands were transferred to nitrocellulose membrane. Immunoblotting was performed with sera of allergic patients to mosquito bites, then completed by antibodies to human IgE. Results : Immunoblotting showed IgE binding proteins weighing 70 kD, 60 kD, 34 kD in Culex pipiens. Conclusion : We found that mosquito antigens induced IgE response, and it suggested that species-specific antigens exist. Further investigation using salivary gland extracts from Culex genus are needed in identifying specific allergens.

치조골 흡수가 포오스트로 인한 치근내 응력에 미치는 영향에 관한 삼차원 유한요소법 분석

이기영,장익태,Lee, Ky-Young,Chang, Ik-Tae 대한치과보철학회 1997 대한치과보철학회지 Vol.35 No.4

There're many cases that should be reconstructed with post and core when clinical crown is destructed. But this post and core restoration may cause damaging stress on the teeth. Previous finite element study was restricted to normal bone model relatively close to cemen-toenamel junction. Moreover, the test of a model with diminished bone support was rare. The purpose of this study is to test the effects of alveolar bone loss on the magnitude, stress distribution and displacement of post reconstructed teeth. In this study, it was assumed that the coronal portion of upper incisor was severely destructed. After conventional endodontic treatment, it was restored with post and core. The PFM restoration was made on it. This crown was cemented with ZPC. Alveolar bone was classified by 4 types of bone, such as normal, 2 mm, 4 mm, 6 mm bone, according to the bone loss. Meanwhile, the material of post are divided into 2 types of materials, such as gold, co-cr. Force was applied to two directions. One was fuctional maximum bite force (300 N) applied to the spot just lingual to the incisal edge with the angle of 45 degree to the long axis of the tooth, and the other one was horizontal force (300 N) applied to the labial surface. The results analyzed with three dimensional finite element method were as follows : 1. Stress was concentrated on the adjacent dentin of the post apex, one third portion of the post apex and the labial & lingual mid-portion of the root in all case. The stress of middle third of the root was apparently concentrated on the labial aspect. 2. The stress on adjacent dentin of the post apex and one third of the post apex increased as alveolar bone height moved apically. This increase was dramatic beyond 4 mm bone loss model. 3. The stress of the post apex was spreaded to the middle third of the post and greater than gold post in the case of metal post. 4. The displacement of the neck of post was the greatest in one of the post-cement interface and this increased as alveolar bone height moved apically. Besides the displacement of the metal post is slightly lower than one of the gold post.

무인잠수정 기반 기뢰대항전체계 개발을 위한 소요기술 분석

이기영,Lee, Ki-Young 한국군사과학기술학회 2011 한국군사과학기술학회지 Vol.14 No.4

One of the most significant UUV(Unmanned Underwater Vehicle) applications is MCM(Mine Countermeasure), which makes good use of UUV characteristics to provide covert, rapid, controlled and efficient survey of a potential minefield without risking a human operator. In this paper, a survey of the today's MCM missions where UUVs will play a role, the vehicle systems that are either under development or planned in the future are presented. And examines principal technical challenges and outline new enabling technologies. Particularly, this paper analyses current approaches to tacking these technologies and technological limitation of UUVs as a MCM platform, and research efforts to develop the technology necessary to meet the domestic MCM mission needs.

부부의 공유여가시간과 결혼만족도

이기영,김외숙,구혜령 ( Ki Young Lee,Oi Sook Kim,Hye Ryung Koo ) 대한가정학회 1995 Human Ecology Research(HER) Vol.33 No.3

논문편 : 관리기능에 대한 체계론적 접근

이기영,조영희 ( Ki Young Lee,Young Hee Cho ) 대한가정학회 1992 Family and Environment Research Vol.30 No.4

고속도로 차로통행기준제도 고찰

이기영,김태호,Lee, Gi-Yeong,Kim, Tae-Ho 대한교통학회 2009 교통 기술과 정책 Vol.6 No.1

낮은 속도비에서의 직렬 이중 제트-교차흐름의 유동 구조

이기영,Lee, Ki-Young 한국군사과학기술학회 2015 한국군사과학기술학회지 Vol.18 No.4

The flow field structures of dual jet-in-cross-flow were examined experimentally for in-lined perforated damage holes configuration using particle image velocimetry. Ensemble averaged in-plane velocity and vorticity data in the jet were determined to study the mean jet structure. Jets are formed by pressure differences between upper and lower airfoil surface. The flow structure of vicinity of the thru holes consist of a vortical structure that wrap around the jets like a horseshoe and develop further downstream through a pair of stream-wise vortices. The shape, size and location of the horseshoe vortex were found to be dependent on the angle of attack. In spite of the existence of battle damage holes, the effect on the control force was insignificant when the damage size was not large enough.

무인헬기 - 헬기처럼 이륙해 비행기처럼 난다

이기영,Lee, Gi-Yeong 한국과학기술단체총연합회 2011 과학과 기술 Vol.508 No.-

과학기술이 가사노동수행양식에 미친 영향 - 가사노동의 기계화를 중심으로 -

이기영,김성희 ( Ki Young Lee,Sung Hee Kim ) 대한가정학회 1995 Human Ecology Research(HER) Vol.33 No.1

사상멤버쉽함수에 의한 화자적응 단어인식

이기영,최갑석,Lee, Ki-Yeong,Choi, Kap-Seok 한국음향학회 1992 韓國音響學會誌 Vol.11 No.3

본논문에서는 불특정화자 음성인식의 문제점이 되는 개인차에 의한 변동을 흡수하기 위하여 사상멤버쉽함수에 의한 화자적응 단어인식 방법을 제안하였다. 이방법의 학습과정에서는 미지화자의 표준화자의 스펙트럼패턴 사이에서 작성된 사상코드북에 퍼지이론을 도입하여 사상멤버쉽함수를 작성하였으며, 인식과정에서는 미지화자의 음성패턴을 사상멤버쉽함수에 의해 표준화자의 음성패턴에 적응된 패턴으로 재구성하고 뉴럴-퍼지패턴매칭에 의해 단어를 인식하였다. 본 방법의 타당성을 평가하기 위하여, 28개의 DDD 지역명을 대상으로 실험한 결과, 종래의 사상코드북에 의한 벡터양자화 화자적응방법에서는 64.9[%], 퍼지벡터양자화 화자적응방법에서는 76.1[%]의 인식율을 얻었으나, 사상멤버쉽함수에 의한 화자적응방법에서는 95.4[%]의 향상된 인식율을 얻으므로써 인식성능의 우수함을 확인하였다. 또한 사상멤버쉽함수의 작성과정에서는 반복된 학습과정이 불피요하며, 기억용량과 계산량도 사상코드북에 의한 화자적응방법보다 각각 1/30, 1/500배 정도였다. In this paper, we propose the speaker adaptive word recognition method using a mapped membership function, in order to absorb a fluctuation owing to personal difference which is a problem of speaker independent speech recognition. In the training procedure of this method, the mapped membership function is made with the fuzzy theory introducded into a mapped codebook, between an unknown speaker's spectrum pattern and a standard speaker's one. In the recognition procedure, an input pattern of an unknown speaker is reconstructed to the pattern which is adapted to that of a standard speaker by the mapped membership function. To show the validity of this method, word recognition experiments are carried out using 28 DDD area names. The recognition rate of the conventional speaker-adaptive method using a mapped codebook by VQ is 64.9[%], and that made by a fuzzy VQ is 76.2[%]. Throughout the experiment using a mapped membership function, we can achieve 95.4[%] recognition rate. This shows that our proposed method is more excellent in recognition performance. Moreover, this method doesn't need an iterative training procedure to make the mapped membership function, and memory capacity and computation requirements for this method are reduced to 1/30 and 1/500 time of those for the conventional method using a mapped codebook, respectively.