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DC, pulse 조건에 따른 구리 도금층 미세 조직 관찰
윤지숙,박찬수,홍순현,이현주,이승준,김양도,Yoon, Jisook,Park, Chansu,Hong, Soonhyun,Lee, Hyunju,Lee, Seungjun,Kim, Yangdo 한국재료학회 2014 한국재료학회지 Vol.24 No.2
The aim of this work was to investigate the effects of electrodeposition conditions on the microstructural characteristics of copper thin films. The microstructure of electroplated Cu films was found to be highly dependent on electrodeposition conditions such as system current and current density, as well as the bath solution itself. The current density significantly changed the preferred orientation of electroplated Cu films in a DC system, while the solution itself had very significant effects on microstructural characteristics in a pulse-reverse pulse current system. In the DC system, polarization at high current above 30 mA, changed the preferred orientation of Cu films from (220) to (111). However, Cu films showed (220) preferred orientation for all ranges of current density in the pulse-reverse pulse current system. The grain size decreased with increasing current density in the DC system while it remained relatively constant in the pulse-reverse pulse current system. The sheet resistance increased with increasing current density in the DC system due to the decreased grain size.
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Monitoring of the amphetamine-like substances in dietary supplements by LC-PDA and LC–MS/MS
윤지숙,권기성,최장덕,조천호 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.5
Recently, amphetamine-like substances derived from the b-phenylethylamine core structure have been detected in dietary supplements. Especially, bmethylphenylethylamine (BMPEA), an amphetamine isomer, has been found in dietary supplements labeled as containing Acacia rigidula. The U. S. Food and Drug Administration determined that BMPEA is not naturally present in food and does not meet the statutory definition of a dietary ingredient. In addition, BMPEA has been classified as a psychotropic drug in South Korea and a doping substance by the World Anti-Doping Agency. The aim of this study was to determine whether dietary supplements contained amphetamine and amphetamine-like substance, including b-phenylethylamine (b-PEA) and BMPEA using LC-PDA and LC–MS/MS. In 10 of 110 samples, illegally added compounds were detected in the following ranges; b-PEA 1.4–122.0 mg/g and BMPEA 4.7–37.6 mg/g. This study will contribute to enhancement of food safety in the South Korea.
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LC-MS/MS를 이용한 식품 중 천속단과 한속단 지표성분 동시분석
윤지숙(Jisuk Yun),김진영(Jinyoung Kim),최장덕(Jangduck Choi),권기성(Kisung Kwon),조천호(Cheon-Ho Jo) 한국식품과학회 2016 한국식품과학회지 Vol.48 No.6
천속단과 한속단은 명칭이 유사하여 ‘속단’으로 통칭하여 판매되고 있으나, 종과 속이 서로 다른 약재일 뿐만 아니라 함유 성분이 다르므로 주의가 필요하다. 천속단은 골격강화, 골절치료 작용이 있는 한약재로 식품원료로는 사용할 수 없으나, 한속단의 뿌리와 잎은 식품원료로 사용이 가능하다. 본 연구에서는 식품중 천속단 혼입여부 판별을 위하여 LC-MS/MS를 이용한 동시분석법을 개발하였으며, 선택성, 직선성, 검출한계, 정량한계, 정확성, 정밀성을 검토하였다. 국내 유통중인 식품 중 어린이 키성장을 표방하는 제품 17건을 구매하여 천속단 혼입여부에 대한 실태조사를 하였다. 분석결과 17건의 시료 중 5건의 시료에서 천속단이 검출되었으며, 천속단 지표성분의 함량은 loganin 0.19-14.45 μg/mL, sweroside 0.13-4.61 μg/mL, akebia saponin D 0.59-19.29 μg/mL이었다. 본 연구에서 개발된 동시분석법은 식품 중 불법 혼입된 천속단을 간단하면서도 신속하게 판별하는데 도움이 될 것으로 사료된다. Phlomis umbrosa and Dipsacus asperoides are distinct species, even though they have a similar appearance. Dipsacus asperoides is used as a Chinese medicinal plant for and has bone strengthening and fracture healing but Phlomis umbrosa has no effect on bone growth. Recently, these plants were used in children’s food to improve their bone growth, without distinction in food. Intakes of Dipsacus asperoides in food may be dangerous, because it has never been used in food and its safety has never been tested in humans. We developed liquid chromatography with tandem mass spectrometry method to distinguish these plants in food. The method was validated for linearity, limits of detection, limits of quantification, accuracy and precision. In 5 of 17 samples, we identified Dipsacus asperoides, containing loganin 0.19-14.45 μg/mL, sweroside 0.13-4.61 μg/mL and akebia saponin D 0.59-19.29 μg/mL. The developed method might be useful to identify Dipsacus asperoides in adulterated food.
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윤지숙(Jisuk Yun),최장덕(Jangduck Choi),권기성(Kisung Kwon),조천호(Cheon-Ho Jo) 한국식품과학회 2016 한국식품과학회지 Vol.48 No.5
성기능개선, 원기증강 등에 효과가 있는 것으로 판매되고 있는 식품 등에서 발기부전치료제 및 그 유사물질 등이 불법적으로 혼입되어 검출되고 있다. 이들 부정물질이 혼입된 불량식품은 안전성과 유효성이 입증되지 않았기 때문에 국민건강을 위협할 수 있다. 그러므로 본 연구에서는 식품과 가짜 발기부전치료제에 불법 혼입된 부정물질을 모니터링하기 위하여, 해외 인터넷 사이트와 오프라인 매장에서 성기능개선, 원기증진을 표방하는 식품과 최음제, 가짜 발기부전치료제 161건을 구입하였다. 시험방법의 유효성 검증을 위하여 선택성, 직선성, 검출한계, 정량한계, 정확성, 정밀성을 검토하였다. LC-PDA와 LC-MS/MS를 이용하여 부정물질 54종을 동시분석한 결과, 48건의 시료에서 7종의 부정물질(요힘빈, 이카린, 실데나필, 타다라필, 클로로프레타다라필, 데메틸실데나필, 디메틸치오실데나필)이 검출되었다. 성기능개선이나 최음제로 판매되는 제품에는 부정물질이 혼입될 가능성이 있으므로, 이들 제품을 구입할 때는 각별한 주의가 필요하다. Illegally adulterated compounds, such as impotency drugs and their synthetic analogues, which have been purported to enhance sexual potency or mood, have been constantly detected in foods including dietary supplements. The adulterated foods with these illegal compounds may threaten public health because their safety and efficacy have not been verified. This study was aimed at investigating illegal compounds in foods and counterfeit products. 54 illegal compounds were assayed using a simultaneous analytical method involving liquid chromatography equipped with photo diode array (LC-PDA) and LC coupled with tandem mass spectrometry (LC-MS/MS). The method was validated in terms of selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy. In 48 of 161 samples, we identified 7 different illegal compounds, including sildenafil, tadalafil, chlropretadalafil, demethylsildenafil, dimethylthiosildenafil, icariin and yohimbine. When purchasing products marketed for erectile dysfunction or aphrodisiacs, ulmost care should be taken owing to the possible presence of these illegal compounds.
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식품 중 부정 혼입된 비만치료제 및 사용금지 성분 실태조사
윤지숙(Jisuk Yun),최장덕(Jangduck Choi),권기성(Kisung Kwon),조천호(Cheon-Ho Jo) 한국식품과학회 2016 한국식품과학회지 Vol.48 No.5
비만은 전세계적으로 심각한 문제로 인식되고 있다. 최근 체중감소와 유지를 위한 다이어트제품에 비만치료제와 그 유사물질, 사용금지 성분들이 발견되고 있다. 정부의 감시를 피하기 위하여, 비만치료제의 화학구조를 일부 변형시킨 유사물질이 지속적으로 합성되고 있다. 부정물질이 혼입된 다이어트식품을 신속하게 검사하기 위하여, 국내에서 판매되는 제품과 해외 인터넷 사이트에서 128건의 다이어트제품을 구매하였으며, HPLC-PDA와 LC-MS/MS로 21종의 부정물질을 동시분석하였다. 시험법의 유효성 검증은 선택성, 직선성, 검출한계, 정량한계, 정확성, 정밀성을 검토하였다. 분석결과 31건의 시료에서 부정물질이 검출되었으며, 검출수준은 시부트라민 9.9-135.3 mg/g, 요힘빈 0.2-17.5 mg/g, 이카린 1.8 mg/g이었다. 본 연구의 부정물질 분석법은 간단하면서도 신속한 분석법으로, 식품안전관리를 위한 기초자료로 활용될 수 있을 것으로 사료된다. Globally, obesity has been recognized as a serious health problem. Recently, slimming foods for weight loss and maintenance were found to contain anti-obesity drugs, their analogues, and prohibited ingredients. To avoid inspections by the Government, structurally modified analogs have been continuously synthesized. For rapid determination of adulterated slimming products, we simultaneously analyzed 21 illegal compounds using liquid chromatography (LC) equipped with photo diode array, and LC coupled with tandem mass spectrometry. The validation of the method was performed with regard to selectivity, linearity, limit of detection, limit of quantification, precision, and accuracy. We purchased 128 samples from the Korean market and online sources in the year 2015. In 31 samples, 3 illegal compounds were detected as follows: 9.9-135.3 mg/g of sibutramine, 0.2-17.5 mg/g of yohimbine, and 1.8 mg/g of icariin. This simultaneous detection method might prove to be a simple and rapid analysis for monitoring illegal compounds in slimming foods.
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김혜경,윤지숙 한국교원교육학회 2018 한국교원교육연구 Vol.35 No.1
본 연구는 급변하는 미래사회의 좋은 미술교사의 역량에 대한 교사의 관점을 파악하기 위 한 것이다. 이를 위해 선행연구 탐색을 통해 좋은 교사의 의미를 탐색하였다. 또한 기존 연구 에서 좋은 교사의 의미에 대한 이견들에 대하여 미술교사를 대상으로 설문지와 인터뷰를 통 해 다양한 의견을 수렴하였다. 연구결과, 미래사회에서의 교육은 학습 환경과 교육환경의 변 화에 따라 학교의 역할, 교수 학습의 방법, 학습자와 교사의 역할이 변화 될 것이라고 예측되 었다. 또한 사회 문화적 변화를 통하여 교육 협력적 상황이 강조되고, 지식전달자의 입장에서 조력자의 역할을 강조하는 미래사회의 미술교사가 좋은 교사라는 공통된 의견이 도출되었다. 이 연구는 그동안 진행된 좋은 교사에 대한 연구들과 비교하여 미술교과의 특성을 반영한 ‘좋은 미술교사’에 대한 접근을 통해 미술교사가 생각하는 ‘좋은 미술교사’의 의미를 탐색했다 는 점에서 의의를 갖는다. This study was to find out a good art teacher’s competences for the rapidly changing future society. To achieve this purpose, the meaning of a good teacher was explored using advanced researches. And also, art teachers’ diversity of opinions of existing research about good art teacher’s different view were collected by using questionnaire and interview for current art teachers. As a result, a following research results were drawn; a role of school education, teaching and learning methods, roles of learners and teachers might be changed according to changes of learning and education environment in the future society. Besides, a common ground that a good art teacher will emphasize educational cooperation, and his(her) role will be not knowledge transmitter but learner assistance in the future was found out. This study was meaningful in terms of exploring the a good art teacher’s meaning reflecting characteristics of art teacher throughout their views different from advanced researches about a good teacher.
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이광옥,윤지숙 이화여자대학교 간호과학연구소 2001 Health & Nursing Vol.13 No.2
The purpose of this study is to develop the community health service center program through community assessment results. The tool used include community character, health level, community resource dimension. The subjects of the study are 645 client in service area. Data are collected from survey, interview, oversight, literary review, institution visiting. The results of the study are as follows; 1. The accident prevention program is needed for the potential risk of accidents related to resident structure. 2. The leisure program for the elderly is needed for psychological health of them. 3. The preventive health care program is needed for their client health prevention. 4. The education program is needed for prevention of health threatening factors such as insufficient exercise. 5. The program of disease group counseling and disease education is needed for sick person. 6. The advertising program of the community health service center program is needed for potential clients. 7. The visit nurse program for the living alone elderly is needed for their health management.
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