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유용운,홍성운,김태환,Ryu, Yong-Wun,Hong, Seong-Woon,Kim, Tae-Hwan 한국현미경학회 1989 Applied microscopy Vol.19 No.2
To investigate the research of the tumor seeker using liposomes with negatively stained. The fine structure and size in liposomes composed of PC, DSPC, DAPC and SM phospholipid were observed. And the yield of vesicle affected with sonication by mechanical force were determined. The results were as follows. 1. The stain of 2% UA are obtained a good resolution from electron microscopic observation to compare the negatively liposomes with PTA, AM and UA solution. 2. The fine structure of liposomes except DAPC, PC and CH alone samples could be observed from EM experiment of liposomes composed of PC, DSPC, DAPC and SM phospholipid. 3. The results of experiment from 10, 20, 40 and 60 times sonication with 30sec, prepared 0.1mM NTA-SM-liposomes were obtained unilamella vesicles from groups of multilamella vesicles of phospholipid. 4. About 50nm diameter liposomes obtained through membration filtration step to prepare homogenized liposomes.
Furosemide 이뇨중의 내당능 및 Insulin 반응에 미치는 K 또는 Ca 보충투여의 영향
성호경(Sung, Ho-Kyung),고주환(Koh, Joo-Hwan),유용운(Ryu, Yong-Woon) 대한생리학회 1976 대한생리학회지 Vol.10 No.2
Effect of furosemide administration on glucose tolerance, insulin-and HGH response following parenteral glucose loading was studied in healthy subjects. Effects of potassium or calcium on the furosemide induced changes were also observed in the same subjects. Furosemide administration resulted in a considerable reduction in the magnitude and duration of insulin response although no obvious changes were observed in glucose disappearance from the circulation. Oral potassium or calcium supplement to the furosemide treated subjects showed a reversion toward normal of the insulin response. The author suggested that a decreased endogenous insulin production resulting from the potassium and/or calcium depletion is responsible for the changes observed. In those subjects who receive parenteral nutrition if administration of furosemide is essential, it should be supplemented by potassium and/or calcium.