Comparison of Seasonal Heavy Metal Concentrations of Total Suspended Particles in the Environmental Atmosphere

유석민,변진여,이명섭,조민정,이지현,장유라,김세은,정인영,박경화,신선경,박유미 한국분석과학회 2023 학술대회논문집 Vol.2023 No.11

성견에서 발치 후 즉시 식립 임플란트에 설치한 자석이 주위 조직에 미치는 영향

유석민,조인호,신수연,Yu, Seok-Min,Cho, In-Ho,Shin, Soo-Yeon 대한치과보철학회 2009 대한치과보철학회지 Vol.47 No.4

연구목적: 전기장과 전자기장은 1970년대부터 여러 기술들이 개발되어 골절의 치유가 어려운 경우에도 치유를 촉진하는 것으로 알려져 왔다. 한편 임플란트 술식이 성공하려면 임플란트가 견고하게 골과 결합하여 오랫동안 기능성 부하에 견딜 수 있어야 한다. 그러나 이 과정은 통상 6-12개월의 오랜 기간이 소요되며, 발치 후에는 치조제가 전반적으로 감소하며 치조제의 근단측, 협설측 흡수가 일어난다. 그래서 이러한 문제점들을 극복하기 위해 임플란트의 즉시 식립이 제시되었다. 그러나 여전히 치아 상실 후 즉시 임플란트를 식립하여도 발치와 함께 일어나는 골개조가 억제되지는 않았고, 치아 발거 후에는 치조제 높이가 지속적으로 감소한다고 하였다. 이에 본 연구에서는 이러한 가설 즉, 정적 자기장을 형성하는 영구자석을 임플란트 즉시 식립 후 임플란트 상부에 설치하여 신선 발치와에서 생리적으로 일어나는 조직개조에 의한 골흡수를 억제시킬 수 있는지를 알아보고자 하였다. 연구재료 및 방법: 임플란트는 직경 4.0 mm, 길이 8.5 mm로 실험군, 대조군 각각 8개씩 총 16개를 실험에 이용하였다. 30 kg 전후 성견 4마리의 하악 양측 제 3, 4 소구치 발거 후 임플란트를 즉시 식립하였고, 실험군은 임플란트 상부에 자석을 부착한 후에, 대조군은 임플란트 상부에 cover screw을 연결한 후에 결손부에 골이식재나 차폐막 없이 판막을 재 위치시키고 봉합하였다. 형광현미경 관찰을 위하여 식립 1주, 6주, 11주에 각각 oxytetracycline hydrochloride, calcein, 그리고 alizarin red S 순서로 정맥주사 하였다. 12주의 치유과정을 거친 후 희생시켜 조직 시편을 제작하였고 광학현미경과 형광현미경 하에서 골-임플란트 접촉율 및 골면적율을 측정하고 치조골 흡수량을 측정하여 관찰하였다. 결과 및 결론: 골접촉율 측정 결과 설측에서의 골접촉율 비교시 유의성이 없었으나 협측에서는 실험군이 유의성 있게 높았다 (P<.05). 골면적율 측정 결과 실험군이 대조군에 비해 높았으나 유의성은 없었다. 또한 치조정 높이의 소실은 실험군이 대조군에 비해 유의하게 더 적었고 (P<.05), 협설골벽의 치조정 높이의 소실은 협측이 설측에 비해 유의하게 더 컸다 (P<.05). 이상의 결과로 볼 때 성견 하악에서 발치 후 즉시 임플란트 식립시 설측벽에 비해 협측벽의 골소실이 다소 크나, 발치 후 즉시 임플란트를 식립하고 자석을 부착할 경우 골형성에 유리한 조건을 제공하여, 치아 발거 후 발생하는 생리적인 골개조 반응으로 인한 골흡수를 최소화할 뿐만 아니라 임플란트 안정과 성공에 기여할 수 있을 것으로 생각된다. Statement of problem: The clinical use of electric and electomagnetic fields for fracture healing applications began in the early 1970s. Since then, several technologies have been developed and shown to promote healing of fractures. Developments of these devices have been aided in recent years by basic research and several well controlled clinical trials not only in the medical field but in dentistry. Purpose: The purpose of this study was to compare alveolar bone reduction following immediate implantation using implants onto which magnets were attached in fresh extracted sockets. Material and methods: Four mongrel dogs were involved. Full buccal and lingual mucoperiosteal flaps were elevated and third and fourth premolars of the mandible were removed. Implants with magnets and implants without magnets were installed in the fresh extracted sockets and after 3 months of healing the animals were sacrificed. The mandibles were dissected and each implant sites were sampled and processed for histological examination. Results: The marginal gaps that were present between the implant and walls of the sockets at the implantation stage disappeared in both groups as a result of bone fill and resorption of the bone crest. The buccal bone crests were located apical of its lingual counterparts. At the 12 week interval the mean of marginal bone resorption in the control group was significantly higher than that of the magnet group. The majority of specimens in magnet group presented early bone formation and less resorption of the buccal marginal bone compared to the control group. Conclusion: Within the limitations of this study, it could be concluded that implants with magnets attached in the early stages of implantation may provide more favorable conditions for early bone formation and reduce resorption and remodeling of marginal bone.

납 안정동위원소를 이용한 아연제련시설 인근지역의 납 오염 특성

유석민 ( Suk-min Yu ),김혁 ( Hyuk Kim ),박유미 ( Yu-mi Park ),박광수 ( Kwang-su Park ),박진주 ( Jin-ju Park ),김지영 ( Jee-young Kim ),석광설 ( Kwang-seol Seok ),김영희 ( Young-hee Kim ) 한국환경분석학회 2016 환경분석과 독성보건 Vol.19 No.3

This study was conducted to evaluate lead(Pb) pollution near a zinc smelter using Pb stable isotopes as environmental tracers. Environmental samples such as air particles, precipitation, soils, conifer leaves, and tree rings were analyzed; input and output materials were also collected from the zinc smelter. The 207Pb/206Pb ratios of the output materials from the zinc smelter were in the range of 0.865-0.881, and plotted on a mixing line with zinc ores from South America and Australia as the two end-members. The 207Pb/206Pb ratios of air particles, precipitation, and conifer leaves were in the ranges of 0.822-0.887, 0.853-0.882, and 0.86 -0.877, respectively, and fall within the line attributed to the smelter. In contrast, the 207Pb/206Pb ratios of soils fell within a broader range of 0.694-0.893. Soils with a higher 207Pb/206Pb ratio of 0.850 were plotted on the smelter line and showed high Pb concentrations of 207.5±141.9 mg/kg. Soils with a lower 207Pb/206Pb ratio of 0.850 were plotted on the Korean ore line and showed low Pb concentrations of 60.5±44.2 mg/kg. The 207Pb/206Pb ratios of tree rings showed an increasing trend toward the isotopic composition of emission gases, implying that the source of Pb pollution changed from the local mine to the zinc smelter.

대도시 보건소 동단위 지역담당 방문보건 시범사업 성과 평가

유호신,황라일,석민현,진달래 한국보건간호학회 2005 한국보건간호학회 학술대회지 Vol.2005 No.12

닭 췌장 Protein Methylase II의 분리정제 및 성질

유태무(Tae Moo Yoo),남궁석민(Suck Min Namkoong),홍성렬(Sung Youl Hong),이향우(Hyang Woo Lee) 대한약학회 1991 약학회지 Vol.35 No.6

Protein methylase II (S-adenosyl-L-methionine:protein carboxyl-0-methyltransferase; EC 2.1.1.24., PM II) was purified from chicken pancreas by subcellular fractionation, DEAE-cellulose chromatography, QAE-Sephadex A-50 chromatography, Sephadex G-75 chromatography, and Sephadex G-75 rechromatography. The purified PM II gave a single band upon polyarcrylamide gel electrophoresis both in the presence of SDS and in Tris glycine buffer without SDS. The pI value of purified PM II was identified as 5.7 on isoelectric focusing gel. Properties and activities of PM II were studied and the following results were obtained. 1) PM II from chicken pancreas was purified approximately 221-fold with a yield of 1.3%. 2) The purified PM II appear constituted of a single polypeptide chain of a molecular weight 46,800 daltons. 3) Hemoglobin exhibited the highest of methyl-accepting activity among the substrates tested. 4) The purified PM II has a Km of 4.67 X 10-6M and a Vmax of 37.5 pmoles of methyl-14C/min/mg enzyme for SAM-14CH3 as methyl donor in the presence of histone type II-As. 5) It is found that S-adenosyl-L-homocysteine is a competitive inhibitor for PM II with KI value of 3.23 X 10-5M.

기능획득 돌연변이 인삼 모상근의 대량생산

유장렬,고석민,인동수,정화지,최동욱 한국식물생명공학회 2007 JOURNAL OF PLANT BIOTECHNOLOGY Vol.34 No.4

This study describes conditions for the mass production of activation-tagged mutant hairy root lines of ginseng by cocultivation with Agrobacterium rhizogenes. Because it is not currently possible to produce progeny from transgenic ginseng, a loss-of-function approach for functional genomics cannot be appliable to this species. A gain-of-function approach is alternatively the choice and hairy root production by cocultivation of A. rhizogenes would be most practical to obtain a large number of mutants. Various sources of explants were subjected to genetic transformation with various strains of A. rhizogenes harboring the activation-tagging vector pKH01 to determine optimum conditions for the highest frequency of hairy root formation on explants. Petiole explants cocultivated with A. rhizogenes R1000 produced hairy roots at a frequency of 85.9% after 4 weeks of culture. Conditions for maximum growth or branching rate of hairy roots were also investigated by using various culture media. Petiole explants cultured on half strength Schenk and Hildebrandt medium produced vigorously growing branched roots at a rate of 2.6 after 4 weeks of culture. A total of 1,989 lines of hairy root mutants were established in this study. These hairy root lines will be useful to determine functions of genes for biosynthesis of ginsenosides.

기능획득 돌연변이 인삼 모상근의 대량생산

고석민,인동수,정화지,최동욱,유장렬,Ko, Suk-Min,In, Dong-Soo,Chung, Hwa-Jee,Choi, Dong-Woog,Liu, Jang-Ryol 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.4

본 연구는 아그로박테리움 공동배양법을 이용한 기능획득 인삼 모상근의 대량생산을 위한 조건 확립에 대한 것이다. 일반적으로, 인삼과 같이 형질전환을 통한종자의 확보가 어려운 식물에서는 loss-of-function을 이용한 기능유전체 연구에 한계가 있다. 한편, 유전자의 기능을 활성화시키는 방법 (gain-of-function)인 activation tagging 기술은 이러한 문제점을 극복할 수 있는 대안이 될 수 있으며, Agrobacterium rhizogenes를 이용한 모상근 생산 시스템은 대량의 돌연변이체를 안정적으로 용이하게 얻을 수 있다는 점에서 최적의 시스템이라고 할 수 있다. 본 연구에서는 activation-tagging된 효율적인 형질전환 모상근 생산에 있어서의 최적의 아그로박테리움 균주 및 인삼조직, 배지조성 등에 대한 조건을 확립하였으며, 다양한 배지에서의 형질전환 모상근의 생장률 및 분지율, 표현형 등을 조사하였다. 엽병 절편을 activation-tagging vector pKH01을 가지고 있는 A. rhizogenes R1000와 공동배양하였을 때 배양 4주후 85.9%의 빈도로 모상근이 생산되었다. 모상근의 최대 생장과 분지도를 나타내는 배양조건을 조사한 바 엽병절편을 1/2 SH 배지에서 4주 배양하였을 때 왕성하게 생장하였으며 2.6의 분지도를 보여주었다. 최종적으로 1,989개체의 독립적인 형질전환 모상근 line을 생산하였으며, 이들 모상근 line은 인삼 진세노사이드 생합성 관련 유전자의 발굴 및 기능해석에 유용하게 쓰일 것이다. This study describes conditions for the mass production of activation-tagged mutant hairy root lines of ginseng by cocultivation with Agrobacterium rhizogenes. Because it is not currently possible to produce progeny from transgenic ginseng, a loss-of-function approach for functional genomics cannot be appliable to this species. A gain-of-function approach is alternatively the choice and hairy root production by cocultivation of A. rhizogenes would be most practical to obtain a large number of mutants. Various sources of explants were subjected to genetic transformation with various strains of A. rhizogenes harboring the activation-tagging vector pKH01 to determine optimum conditions for the highest frequency of hairy root formation on explants. Petiole explants cocultivated with A. rhizogenes R1000 produced hairy roots at a frequency of 85.9% after 4 weeks of culture. Conditions for maximum growth or branching rate of hairy roots were also investigated by using various culture media. Petiole explants cultured on half strength Schenk and Hildebrandt medium produced vigorously growing branched roots at a rate of 2.6 after 4 weeks of culture. A total of 1,989 lines of hairy root mutants were established in this study. These hairy root lines will be useful to determine functions of genes for biosynthesis of ginsenosides.

단백질 메틸화효소류 및 S-아데노실-L-메치오닌 연결효소의 활성도에 미치는 사염화탄소-유발 간독서의 영향

남궁석민(Suck Min Namkoong),유태무(Tae Moo Yoo),홍성렬(Sung Youl Hong),이향우(Hyang Woo Lee) 대한약학회 1992 약학회지 Vol.36 No.1

In order to test relationships between hepatotoxicity and transmethylation, activities of protein methylases and SAM (S-adenosyl-L-methionine)-synthetase wee examined in liver tissues of rats treated with CCl4. Also the concentrations of SAM and SAH were measured by HPLC in rat liver. The results are as follows. (1). Activities of protein methylases were not significantly changed in 24 hours after CCl4 treatment. However, in 48 hours, activities of protein methylases were significantly increased in comparison with that of control. (2). Activity of SAM-synthetase was increased steadily in the time course after CCl4 treatment.(3). S-adenosyl-L-methionine concentration of liver tissues in CCl4-treated group was elevated in 24 hours, and then decline thereafter. But the SAH concentration was slightly decreased in the time course after CCl4 treatment. These results indicated that SAM was very actively used in transmethylation reactions of CCl4 damaged rat liver, suggesting the strong relationships between hepatotoxicity and transmethylation phenomena.

대도시지역 동단위 지역담당 방문간호서비스 효과분석 : 뇌졸중 환자를 대상으로

유호신,석민현,황라일,진달래 지역사회간호학회 2005 지역사회간호학회지 Vol.16 No.1

An Evaluation of the Visiting Nursing Service Model Based on the District Management System -focused on stroke patients -Ryu, Ho-Sihn · Suk, Min-Hyun Hwang, Rah-Il · Jin, Dal-Rae Purpose: This study was conducted to examine the effect of visiting nursing of the visiting nursing services based on the district management system on the subjects of stroke patients. Method: A nonequivalent control group pretest-posttest design was applied to 50stroke patients (31 from the experimental group, 19 from the control group). To evaluate the effects of visiting nursing services, health status(SF-36) and activity daily living(ADL/IADL) were measured from June 2003 to November 2004. Result: Health condition and the activities of living (ADL/IADL) of the subjects who received visiting nursing service were shown to be improved. Conclusion: Visiting health service model based on the district management system in public health center is considered to be an effective measure of visiting nursing services in large cities.

Production of Human Serum Albumin in Chloroplast-Transformed Tobacco Plants

유장렬,고석민,Hyun-Chul Kim,Byung-Ho Yoo,우제욱,정화지,Dong-Woog Choi 한국식물생명공학회 2006 JOURNAL OF PLANT BIOTECHNOLOGY Vol.33 No.4

Human serum albumin (HSA) is the most abundant proteinin plasma and is the most often used intravenous protein inmany human therapies. However, HSA is currently extractedonly from plasma because commercially feasible recombinantexpression systems are not available. This studyattempted to develop an efficient system for recombinantHSA production by chloroplast transformation of tobacco. AHSA cDNA was isolated from a cDNA library constructedwith human liver tissue. Chloroplast transformation vectorswere constructed by introducing various regulatory elementsto HSA regulatory sequences. Vectors were delivered byparticle bombardment into leaf explants and chloroplasttransformedplants were subsequently regenerated intowhole plants. Southern blot analysis confirmed that the HSAcDNA was incorporated between rps12 and orf70B of thechloroplast genome as designed. Western blot analysisrevealed that hyper-expression and increasing the stabilityof HSA were achieved by modification of the regulatorysequences using the psbA5'UTRs in combination with elementsof the 14 N-terminal amino acids of the GFP and theFLAG tag. However, only plants transformed with the vectorcontaining all of these elements were able to accumulateHSA.