템플릿 정합법을 이용한 온-오프 형태 DNA 칩의 탐색자 구분

유문호,Ryu, Mun-Ho 한국정보처리학회 2006 정보처리학회논문지B Vol.13 No.6

This paper proposes a nonlinear template matching measure, called counting measure, as a signal detection measure that is defined as the number of on pixels in the spot area. It is applied to classify probes for an on-off type DNA chip, where each probe spot is classified as hybridized or not. The counting measure also incorporates the maximum response search method, where the expected signal is obtained by taking the maximum among the measured responses of the various positions and sizes of the spot template. The counting measure was compared to existing signal detection measures such as the normalized correlation and the median for 2390 patient samples tested on the human papiliomavirus (HPV) DNA chip. The counting measure performed the best regardless of whether or not the maximum response search method was used. The experimental results showed that the counting measure combined with the positional search was the most preferable. 본 연구는 카운팅 정합도라 불리는, 비선형 정합도를 척도로 하는 템플릿 정합법을 이용하여 온-오프 형태 DNA칩의 탐색자를 구분하는 방법을 제안한다. 또한, 스팟 템플릿의 다양한 위치나 크기에 따른 응답의 최대값을 찾는 최대 응답 탐색법을 제안한다. 각 스팟의 교잡반응 여부만을 분류하는 온-오프 형태의 DNA 칩의 탐색자를 구분하는 용도에 제안된 방법을 적용하였다 2390명의 환자에 대한 인유두종 바이러스 DNA 칩 검사에 적용하여, 카운팅 정합도를 기존의 정규상관도나 메디안과 비교하였다. 카운팅 정합도가 최대 응답 탐색법에 관계없이 최고의 성능을 보였다. 실험결과 위치탐색을 결합한 카운팅 정합도가 가장 적절하였다.

템플릿 정합법을 이용한 온-오프 형태DNA 칩의 탐색자 구분

유문호 한국정보처리학회 2006 정보처리학회논문지. 소프트웨어 및 데이터 공학 Vol.13 No.7

This paper proposes a nonlinear template matching measure, called counting measure, as a signal detection measure that is defined as the number of on pixels in the spot area. It is applied to classify probes for an on-off type DNA chip, where each probe spot is classified as hybridized or not. The counting measure also incorporates the maximum response search method, where the expected signal is obtained by taking the maximum among the measured responses of the various positions and sizes of the spot template. The counting measure was compared to existing signal detection measures such as the normalized correlation and the median for 2390 patient samples tested on the human papillomavirus (HPV) DNA chip. The counting measure performed the best regardless of whether or not the maximum response search method was used. The experimental results showed that the counting measure combined with the positional search was the most preferable. 본 연구는 카운팅 정합도라 불리는, 비선형 정합도를 척도로 하는 템플릿 정합법을 이용하여 온-오프 형태 DNA칩의 탐색자를 구분하는 방법을 제안한다. 또한, 스팟 템플릿의 다양한 위치나 크기에 따른 응답의 최대값을 찾는 최대 응답 탐색법을 제안한다. 각 스팟의 교잡반응 여부만을 분류하는 온-오프 형태의 DNA 칩의 탐색자를 구분하는 용도에 제안된 방법을 적용하였다. 2390명의 환자에 대한 인유두종 바이러스 DNA 칩 검사에 적용하여, 카운팅 정합도를 기존의 정규상관도나 메디안과 비교하였다. 카운팅 정합도가 최대 응답 탐색법에 관계없이 최고의 성능을 보였다. 실험결과 위치탐색을 결합한 카운팅 정합도가 가장 적절하였다.

방향을 정돈해 가는 과정의 삶

유문호 샘터사 2000 월간 샘터 Vol.31 No.12

논총(論叢) : 전략특집(戰略特輯) ; 현대지상군(現代地上軍)

유문호 ( Mun Ho Yu ) 국방대학교 안보문제연구소 1958 국방연구 Vol.2 No.-

온-오프 타입 DNA 칩의 자동판독 시스템

유문호 ( Munho Ryu ),김종대 ( Jong-dae Kim ) 한국정보처리학회 2006 한국정보처리학회 학술대회논문집 Vol.13 No.2

본 연구는 진단용 DNA 칩의 자동판독 시스템을 제안하는 것을 목적으로 한다. 일반적인 자동판독 시스템의 사양을 정의하고 그 구현방법을 제안하였다. 응용 예로서 자궁경부암 진단용 DNA칩을 대상으로 GenePix 스캐너 프로그램 환경에 적용하였다. 영상획득은 GenePix 의 라이브러리를 사용하여 HTML 언어로 구현하였고, 영상의 판단과 보고서 생성은 Microsoft Visual C++ 6.0 를 사용하여 COM 형태로 구현하였다. 결과 보고서는 한글 2002 문서에 환자 정보와 결과 정보 등에 해당하는 곳에 미리 정의된 표지문자열들을 삽입하여 템플릿을 만들었다. 판독 시스템은 템플릿을 읽어들여 처리 결과의 내용으로 표지문자열들을 치환하여 보고서를 생성하였다. 제안한 시스템을 통해서 스캐닝을 통한 영상획득, 영상의 판독, 결과 보고서 생성으로 구성된 전체 판독과정이 사용자의 개입 없이 자동으로 처리될 수 있었다. 본 시스템은 기존에 수작업을 자동화여 판독 시간을 단축하고 판독 기준을 정량화하여 진단용 DNA 칩이 대량검사 활용되는데 공헌할 것으로 기대된다.

ZigBee를 이용한 뇌졸중 치료용 무선 전기 자극기 개발

김국화,유문호,신용일,김형일,김남균,양윤석,Kim, G.H.,Ryu, M.H.,Shin, Y.I.,Kim, H.I.,Kim, N.G.,Yang, Y.S. 대한의용생체공학회 2007 의공학회지 Vol.28 No.1

Stroke is the second most significant disease leading to death in Korea. The conventional therapeutic approach is mainly based on physical training, however, it usually provides the limited degree of recovery of the normal brain function. The electric stimulation therapy is a novel and candidate approach with high potential for stroke recovery. The feasibility was validated by preliminary rat experiments in which the motor function was recovered up to 80% of the normal performance level. It is thought to improve the neural plasticity of the nerve tissues around the diseased area in the stroked brain. However, there are not so much research achievements in the electric stimulation for stroke recovery as for the Parkinson's disease or Epilepsy. This study aims at the developments of a wireless variable pulse generator using ZigBee communication for future implantation into human brain. ZigBee is widely used in wireless personal area network (WPAN) and home network applications due to its low power consumption and simplicity. The developed wireless pulse generator controlled by ZigBee can generate various electric stimulations without any distortion. The electric stimulation includes monophasic and biphasic pulse with the variation of shape parameters, which can affect the level of recovery. The developed system can be used for the telerehabilitation of stroke patient by remote control of brain stimulation via ZigBee and internet. Furthermore, the ZigBee connection used in this study provides the potential neural signal transmission method for the Brain-Machine Interface (BMI).

관성 센서와 지자계 센서를 사용한 인체 방향 추적 시스템

최호림,유문호,양윤석,Choi, H.R.,Ryu, M.H.,Yang, Y.S. 대한의용생체공학회 2011 의공학회지 Vol.32 No.2

This study proposes a human body orientation tracking system by inertial and earth magnetic sensors. These sensors were fused by indirect Kalman filter. The proposed tracking system was configured and the filter was implemented. The tracking performance was evaluated with static and dynamic tests. In static test, the sensor was fixed on the floor while its static characteristics was analyzed. In dynamic test, the sensor was held and moved manually for 30 seconds. The dynamic test included x, y, z axis rotations, and elbow flection/extension motions that mimic drinking. For these dynamic motions, the tracking angle error was under $4.1^{\circ}$ on average. The proposed tracking method is expected to be useful for various human body motion analysis.

ZigBee 기반의 무선 뇌 자극기와 네트워크를 이용한 원격 뇌졸중 회복 시스템의 개발

윤효정,양윤석,유문호,김정자,김남균 제어로봇시스템학회 2007 제어로봇시스템학회 합동학술대회 논문집 Vol.2007 No.12

ZigBee-based Wireless Neuro-Stimulator for Improving Stroke Recovery

김국화,윤효정,유문호,김형일,양윤석,신용일 한국뇌신경과학회 2010 Experimental Neurobiology Vol.19 No.3

Stroke is a leading cause of adult disability and the second-leading cause of death in Korea. It is also the third-leading cause of death in the United States, leading to a serious demand for new interventions to improve the quality of life in stroke survivors. To this end, direct cortical stimulation using an epidural electrode has been reported with promising results in animal and human studies, showing the potential for enhancing the recovery in chronic stroke patients. For optimal results, doctors must be able to modify the stimulation pattern as frequently as needed over a period of time for a given patient. However, severe aftereffects caused by stroke limit patients' activities, making regular doctor visits for treatment difficult. This study aims to develop a prototype of a telemedicine system to enhance stroke recovery by using a ZigBee- based wireless neuro-stimulator. The ZigBee is a stable platform for many low-power wireless applications. To allow stroke patients to remotely obtain neuro-stimulation treatments from their doctors, we connected the ZigBee to the internet. The system also allows doctors to personalize treatment based on the history of the stimulation parameters. The system developed here can also be beneficial as a common platform for a wide range of brain diseases and clinical care for which electric stimulation is used.

ZigBee 기반의 무선 뇌자극기를 이용한 원격 뇌졸중 치료 시스템

윤효정,양윤석,유문호,김정자,김남균,Yun, H.J.,Yang, Y.S.,Ryu, M.H.,Kim, J.J.,Kim, N.G. 대한의용생체공학회 2007 의공학회지 Vol.28 No.5

Stroke patients need regular medical treatments and rehabilitation training from their doctors. However, severe aftereffects caused by stroke allow them minimum activities, which make it difficult for them to visit doctor. Recently, electric brain stimulation treatment has been found to be better way compared to conventional ones and many are interested in using this method for the treatment of stroke. In this study, we have developed a remote medical treatment system using wireless electric brain stimulator that can help the stroke patients to get a treatment without visiting their doctors. The developed remote medical treatment system connects the doctors to the brain stimulator implanted in the patients via the internet and ZigBee communication built in the brain stimulator. Also, the system receives personal information of the connected patients and cumulates the total records of electric stimulation therapy in a database. Doctors can easily access the information for better treatment planning with the help of graphical visualization tools and management software. The developed remote medical treatment system can be applied to the electric stimulation treatments for other brain diseases with a minor change.