호흡기 상피세포에서 roxithromycin에 의한 MUC5B와 MUC8 점액 유전자 발현 및 점액 생성의 억제

우현재,민명기,배창훈,송시연,김용대 대한이비인후과학회 2008 대한이비인후과학회지 두경부외과학 Vol.51 No.7

Macrolide antibiotics are known to inhibit mucus hypersecretion in patients with chronic airway diseases, but its action mechanism is unclear. Several reports demonstrated that macrolides significantly inhibited gene expression of MUC2, MUC4 and MUC5AC in the airway epithelial cells, but little is known about its inhibitory effect for the other important airway mucins. In upper airway tracts, MUC5B and MUC8 are other important secreted mucin genes. Therefore, this study was aimed to investigate the effects of roxithromycin on the IL-1β-induced gene expression and mucin production of MUC5B and MUC8 in NCI-H292 cells and cultured human nasal polyp epithelial cells. Subjects and Method:The effects of roxithromycin on the IL-1β-induced MUC5B and MUC8 expression were analyzed by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay. Results:Roxithromycin attenuated the IL-1β-induced MUC5B and MUC8 gene expression and mucin production with a dose-dependent pattern in NCI-H292 epithelial cells and cultured human nasal polyp epithelial cells. Conclusion:Roxithromycin exerts direct inhibitory effects on the gene expression of MUC5B and MUC8 in airway epithelial cells. These novel findings may explain the clinical efficacy of 14-membered macrolides in the treatment of chronic airway inflammations. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:617-22)

8세 여아 비강에서 발생한 염증성 근섬유모세포종 1예

우현재,배창훈,김용대 대한이비인후과학회 2008 대한이비인후과학회지 두경부외과학 Vol.51 No.5

Inflammatory myofibroblastic tumor (IMT) is an extremely rare, benign neoplasm, which is composed of myofibroblastic spindle cells along with acute and chronic inflammatory cells. The etiology and pathophysiology of IMT still remains controversial. The diagnosis is made on the base of histologic evidence to exclude other diseases because of its nonspecific clinical manifestations such as fever, anemia, thrombocytopenia, and elevated erythrocyte sedimentation rate. Generally, three different treatment modalities are available:a complete surgical resection, oral steroid and radiation therapy. The authors treated an inflammatory myofibroblastic tumor of the nasal cavity in an 8 year-old girl by surgical resection and we presented the case with a review of the literature. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:481-3)

성대 용종으로 오인된염증성 근섬유모세포종 1예

우현재,배창훈,김용대,송시연 대한이비인후과학회 2008 대한이비인후과학회지 두경부외과학 Vol.51 No.2

Inflammatory myofibroblastic tumor (IMT) is an uncommon benign fibroinflammatory mass that is composed of myofibroblastic spindle cells with acute and chronic inflammatory cells. IMT may arise within numerous organs such as lung, intestine, orbit and paranasal sinuses. However, IMT of the larynx is extremely rare. IMT can have systemic manifestations including fever of unknown origin, anemia, thrombocytosis and elevated erythrocyte sedimentation rate. The treatment of IMT is complete excision, but in some cases, additional therapy such as chemotherapy or radiotherapy may be required. Recently, we experienced a case of IMT which was originated from the right true vocal cord and mimicking the vocal polyp. So, we report this case with a review of the literature. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:206-8)

인체 호흡기상피세포에서 MUC8 발현에 대한 15-Deoxy-Delta12,14-Prostaglandin J2의 억제효과

우현재,배창훈,송시연,김용대 대한이비인후과학회 2008 대한이비인후과학회지 두경부외과학 Vol.51 No.5

Among the numerous mucin genes, MUC8 is regarded as one of the most important mucin genes which are related with upper airway disease such as a nasal polyp. 15-Deoxy-delta12,14-prostaglandin J2 (15d-PGJ2), the most recently discovered prostaglandin, has been known to have multiple cellular functions, including anti-inflammatory and cytoprotective effects. However, the effect of 15d-PGJ2 on mucin gene expression or mucin production has not yet been investigated. The purpose of this study was to determine the effect of 15d-PGJ2 on interleukin-1β (IL-1β)-induced MUC8 gene expression and mucin secretion in the cultured NCI-H292 cells and human nasal polyp epithelial cells. Subjects and Method:The MUC8 mRNA levels were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and MUC8 mucin levels were measured using an enzyme-linked immunosorbent assay (ELISA) in the cultured epithelial cells stimulated by IL-1β. 15d-PGJ2 was added 1 hour before stimulation. Results:15d-PGJ2 attenuated the IL-1β-induced MUC8 mRNA expression and mucin secretion with a dose-dependent pattern in both cultured NCI-H292 cells and human nasal polyp epithelial cells. Conclusion:These results suggest that 15d-PGJ2 may be considered as an effective agent for the control of airway mucus hypersecretion through the down-regulation of MUC8 gene. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:435-40)

비강 및 부비동 악성종양의 내시경적 절제술

우현재,배창훈,송시연,김용대 대한이비인후과학회 2007 대한이비인후과학회지 두경부외과학 Vol.50 No.12

Background and Objectives:Endoscopic surgery largely replaced the traditional external aproach in the treatment of sinonasal inflamatory diseases and most benign tumors. However, there is much debate about its aplicability to resection of sinosal sinonasal malignant tumors. Subjects and Method:We retrospectively reviewed the medical records and radiologic findings of 1 patients who had underwent transnasal endoscopic excision for sinonasal malignancy from 1998 to 2003. Al patients were treated with curative intent. Results:The mean age was 60.6 (31-75 years) years and the mean folow-up period was 69 months (52-112 months)(4 cases), plasmacytoma (2 cases), adenoid cystic carcinoma, olfactory neuroblastoma, cylindrical cel carcinoma, squamous cell carcinoma and neuroendocrine carcinoma. In ten cases, the tumor was removed by transnasal endoscopic excision with ade-quate free margin of normal mucosa. In one case, Caldwel-Luc operation was combined with the endoscopic excision. During the folow-up, only one case of malignant melanoma had recured at the lateral neck and retropharyngeal lymph node, but the Conclusion:Considering oncological results and surgery-related morbidities, transnasal endoscopic resection can be used as a good modality in the highly selected early T stage of sinonasal malignancies. (Korean J Otorhinolaryngol-Head Neck Surg 207 ;50 :1118-24)

AEMFCs 촉매를 위한 유동층 반응기 원자층 증착법을 활용한 Ru/C 촉매에 관한 연구

우현재,이우재,권세훈 한국표면공학회 2021 한국표면공학회 학술발표회 초록집 Vol.2021 No.6

원자층 증착 방법을 활용한 Pt-Ru 바이메탈 합금의 산화 특성에 관한 연구

우현재,권세훈 한국표면공학회 2021 한국표면공학회 학술발표회 초록집 Vol.2021 No.12

원자층 증착법으로 합성된 Pt-Ru bimetallic 합금의 두께에 따른 일함수 영향

우현재,이우재,권세훈 한국진공학회 2020 한국진공학회 학술발표회초록집 Vol.2020 No.8

비·부비동 및 비인두 악성 림프종의 예후인자

우현재,배창훈,송시연,박수찬,김용대 대한이비인후과학회 2008 대한이비인후과학회지 두경부외과학 Vol.51 No.10

There were only few studies for malignant lymphoma of sinonasal tract because of its relatively low incidence. The objectives of this study were to investigate clinical features, treatment outcomes, and prognostic factors in patients with malignant lymphoma of sinonasal tract and nasopharynx. Subjects and Method:Twenty patients diagnosed as sinonasal tract lymphoma from 1995 through 2004 were included in the retrospective analysis. Results:All the patients included in this study were Non-Hodgkin’s lymphoma. The ratio of male to female was 1.6:1, and the ages ranged from 16 to 79 years (mean, 55 years). Nasal cavity was the most frequently involved site (42.3%). NK/T cell lymphoma was the most common histologic subtype (56.3%) in the nasal cavity and paranasal sinus, whereas diffuse B cell lymphoma accounted for 60% of lymphomas involving the nasopharynx. The complete response rate and 5-year survival rate for the entire group were 53.8% and 45.5%, respectively. The predictive factors for poor survival were T-cell type, advanced Ann Arbor stage, single modality treatment, presence of B symptom, high serum LDH level, high Eastern Cooperative Oncology Group (ECOG) performance scale score, and high international prognostic index (IPI) score in the univariate analysis (p<0.05). However, Ann Arbor stage and treatment modality were excluded from prognostic factors in the multivariate analysis (p<0.05). Conclusion:The distribution of histologic subtypes was different according to the site of involvement. Histologic classification, Ann Arbor stage, B symptom, serum LDH level, ECOG performance status, treatment modality, and IPI score are important prognostic factors. (Korean J Otorhinolaryngol- Head Neck Surg 2008;51:888-93)

상부 위장관 내시경 후 발생한 경부 국소농양 1예

우현재,배창훈,김용대,송시연 대한소화기내시경학회 2008 Clinical Endoscopy Vol.37 No.3

상부 위장관 내시경은 비교적 안전한 검사이지만 무리한 조작에 의해 상부 위장관의 천공이 드물게 발생할 수도 있으며 이 경우 대부분 하인두 및 경부식도에서 발생한다. 천공이 발생한 즉시 적절한 치료가 시행되면 인접한 경부공간으로 염증이 파급되지 않고 치유될 수 있지만, 치료의 시작이 늦어질 경우 대부분 경부공간을 타고 하방으로 파급되어 종격동염, 심내막염, 췌장염, 패혈증 등의 치명적인 합병증으로 진행된다. 저자들은 상부 위장관 내시경 시행 직후부터 좌측 경부 통증을 호소한 31세 남자 환자에서, 경부식도 천공에 의한 경부 농양을 조기에 확인하여 보존적인 치료를 통해 성공적으로 치료하였다. 상부 위장관 내시경을 받은 환자가 수일 이상의 지속적인 인후통을 호소하는 경우, 인후두부에 특이소견이 없다고 해서 단순한 일과성 통증으로 간주할 것이 아니라 경부측방촬영을 즉시 시행하여 이상소견의 유무를 확인하거나, 이비인후과와의 긴밀한 협진을 통해 식도손상에 의한 심각한 합병증을 조기에 발견할 수 있도록 노력해야 한다.