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2P-357 Precursor rebalancing for optimal production of isoprenoids in Escherichia coli
우성화,정주영,임현규,김성경,석주연,정규열 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Biosynthesis of isoprenoids via the DXP pathway requires equimolar glyceraldehyde 3-phosphate and pyruvate to divert carbon flux toward the products of interest. Here, we demonstrate that precursor balancing is one of the critical steps for the production of isoprenoids in E. coli. First, the implementation of the synthetic lycopene production pathway as a model system and the amplification of the native DXP pathway were accomplished using synthetic promoters and redesigned 5′-UTR. Next, fine-controlled precursor balancing was investigated by tuning phosphoenolpyruvate synthase (PpsA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The results showed that tuningdown of gapA improved the specific lycopene content by 46% compared to the overexpression of ppsA. The lycopene in the strains with downregulated gapA increased by 96% compared to that in the parental strain. Our results indicate that gapA is the best target for precursor balancing to increase biosynthesis of isoprenoids.
Vibrio sp. dhg; New Powerful Platform for the Biorefinery of Brown Macroalgae
우성화,임현규,서상우,정규열 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Although brown macroalgae holds an enormous potential as an alternative feedstock, its utilization by conventional microbial platforms has been limited due to the inability to metabolize one of the principal sugars, alginate. Here, we isolated Vibrio sp. dhg, a novel, fast-growing bacterium that can efficiently assimilate alginate. Based on systematic characterization of the genomic information of Vibrio sp. dhg, we were able to develop a genetic toolbox for its engineering, and successfully demonstrated its ability to rapidly produce a broad spectrum of chemicals (ethanol, 2,3-butanediol, and lycopene) from brown macroalgae sugar mixtures with a high productivity and yield. Collectively, the Vibrio sp. dhg strain is a powerful platform for the conversion of brown macroalgae sugars whose usage will dramatically accelerate the production of value-added biochemicals.
우성화,임대균,석주연,오민규,정규열 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Biosynthesis of isoprenoids via the DXP pathway requires equimolar glyceraldehyde 3-phosphate and pyruvate to divert carbon flux toward the products of interest. Here, we demonstrate that precursor balancing is one of the critical steps for the production of isoprenoids in E. coli. First, the implementation of the synthetic lycopene production pathway as a model system and the amplification of the native DXP pathway were accomplished using synthetic promoters and redesigned 5'-UTR. Next, fine-controlled precursor balancing was investigated by tuning phosphoenolpyruvate synthase (PpsA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The results showed that tuning-down of gapA improved the specific lycopene content by 46% compared to the overexpression of ppsA. The lycopene in the strains with down-regulated gapA increased by 96% compared to that in the parental strain. Our results indicate that gapA is the best target for precursor balancing to increase biosynthesis of isoprenoids.
Recent Advances in the Utilization of Brown Macroalgae as Feedstock for Microbial Biorefinery
우성화,문조현,성준영,백동엽,손양준,정규열 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.6
A stable and economical supply of biomass is essential for meeting the increasing demand for bioproducts. In this regard, numerous studies on the utilization of macroalgal biomass as feedstock are in progress to exploit its high productivity, high carbohydrate content, low cost, and easy pretreatment. Macroalgal biomass is classified as green, red, and brown depending on the color of its photosynthetic pigments and chlorophyll. Owing to considerable differences in ecology, habitat, and carbohydrate composition, it is necessary to study each macroalgae type as a separate and distinct biomass feedstock. Among them, the utilization of brown macroalgae as a feedstock is expected to not only reduce process costs but also alleviate environmental pollution. Therefore, in this review, we provide a comprehensive and detailed summary of recent advances in the microbial bioconversion of brown macroalgae. Brown macroalgal carbohydrates primarily comprise mannitol, laminarin, alginate, and fucoidan, which require different hydrolases or catabolic enzymes for microbial bioconversion. Therefore, depending on the carbohydrate type, relevant papers were identified and summarized. Given the numerous reports on the successful and efficient utilization of alginate, which is the most abundant carbohydrate in brown macroalgae, we reasoned that the potential of brown macroalgaebased biorefinery is relatively high. Moreover, follow-up studies can contribute to enhance the economic feasibility and commercialization of brown macroalgae-based biorefinery products.
함정 내 가변 음향환경을 고려한 기계학습 기반 능동 소음 제어 연구
우성화,임성훈,한태우,박재호,엄원석 한국소음진동공학회 2023 한국소음진동공학회 논문집 Vol.33 No.5
The effectiveness of active noise control, commonly based on the popular filtered-x least mean square algorithm, depends on the fidelity of secondary path estimation between the secondary source and the error microphone. The corresponding secondary path of a time-varying acoustic environment such as the cabins and compartments of a naval ship is also subjected to a change, which invariably degrades the active noise control (ANC) performance unless the secondary path is recalibrated and updated in real time. This study presents a method for real-time secondary path update based on a deep neural network and investigated its applicability for the active control of machinery noise in a naval ship interior. The method’s feasibility is tested in a one-dimensional airborne duct utilizing actual engine noise recorded in a naval ship. Given some time-varying boundary conditions of the duct, the ANC system with the proposed secondary path update demonstrates excellent noise reduction performance as large as 13 dB.