벼종자 미랑 단백질의 프로테오믹스 연구를 위한 글루테린 저장 단백질의 제거방법

우선희,김세영,김태선,조성우,조건,정근욱,김선림,조용구,김홍식,송범헌,이철원,정승근,박영목,Woo, Sun-Hee,Kim, Se-Young,Kim, Tae-Seon,Cho, Seong-Woo,Cho, Kun,Chung, Keun-Yook,Kim, Sun-Lim,Cho, Yong-Gu,Kim, Hong-Sig,Song, Boem-Heon,Lee, Chul-W 한국작물학회 2006 한국작물학회지 Vol.51 No.suppl1

본 연구는 벼종자 미량 단백질의 프로테오믹스 연구를 위하여 벼종자에 고 함량으로 존재하는 벼종자 글루테린 저장 단백질을 제거하는 방법에 관한 것이다. 따라서 본 연구는, (A) 벼종자에 액체 질소를 가하고 분쇄하여 벼종자 가루를 만드는 분쇄단계; (B) 상기 분쇄된 벼종자 가루를 물에 현탁하여 현탁액을 만드는 현탁단계; (C)상기 현탁액 중 미용해 물질을 제거하는 분리단계를 포함하는, 벼종자 미량 단백질의 프로테오믹스 연구를 위한 벼종자 글루테린 저장 단백질의 제거방법에 관하여 검토하였다. 본 연구의 결과, 단순하고 신속하며 저렴하고 효율적인 방법으로 미량 비글루테린 단백질들을 용이하게 동정할 수 있을 것으로 판단되었다. Abundant proteins often cause problems in proteome study. Glutelin family proteins (hereafter referred to glutelin) are present in rice proteome sample as over-whelming constituents with very high abundance. In order to increase the number of identified proteins in rice proteome study, we developed a newly improved method for sample preparation through the removal of glutelin. When the protein samples from rice seed were extracted by the conventional trichloroacetic acid (TCA) extraction method, glutelin accounts for about 60% of total rice seed proteins in SDS gels. Using our new water extraction method, glutelin consists of only about 10% of total proteins. After analyzing on a two-dimensional gel electrophoresis (2-DE), 937 protein spots were detected using the conventional TCA extraction method. On the other hand, 1240 proteins could be seen using the new water extraction method. The selectivity for non-glutelin and less abundant protein by the water extraction method was also confirmed by ESI-Q/TOF mass spectrometry analysis. Thus, the new water extraction method developed here can be efficiently used to study the proteome analysis of rice storage seed.

프로테옴 해석에 의한 벼 게놈 기능해석과 응용

우선희,김홍식,송범헌,이철원,박영목,정승근,조용구,Woo, Sun-Hee,Kim, Hong-Sig,Song, Berm-Heun,Lee, Chul-Won,Park, Young-Mok,Jong, Seung-Keun,Cho, Yong-Gu 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3

In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is the most prevalent technique to rapidly identify a large number of proteome analysis. However, the conventional Western blotting/sequencing technique has been used in many laboratories. As a first step to efficiently construct protein cata-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein sports are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins(i, e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 45% of total rice cDNA have been deposited in the EMBL database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that tuned out to be calreticulin, gibberellin-binding protein, which is ribulose-1.5-bisphosphate carboxylase/oxygense active in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins(http://genome.c.kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Also, the information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful be in the plant molecular breeding.

이차원 전기영동에 의한 단백질의 분리

우선희,김선림,정승근,김홍식,Woo Sun-Hee,Kim Sun-Lim,Jong Seung-Keun,Kim Hong-Sig 한국작물학회 2004 Korean journal of crop science Vol.49 No.suppl1

점토광물 처리에 따른 토양개량 효과 평가

우선희 ( Sun Hee Woo ),김덕현 ( Deok Hyun Kim ),정근욱 ( Keun Yook Chung ),이석언 ( Seok Eon Lee ),안중우 ( Jung Woo An ) 한국토양비료학회 2011 한국토양비료학회 학술발표회 Vol.2011 No.1

In vitro culture of hybrid ovule between Fagopyrum esculentum Moench and F. homotropicum Ohnishi

Sun Hee Woo(禹仙熙),Taiji Adachi(足立泰二),Seung Keun Jong(鄭丞根) 한국육종학회 1997 한국육종학회지 Vol.29 No.3

Interspecific hybridization in buckwheat is of great importance to transfer desirable genes from wild species to cultivars as well as to broaden genetic variation in cultivars. In vitro ovule culture following the bud pollination was made to overcome postzygotic cross/self-incompatibility in interspecific hybridization between Fagopyrum esculentum Moench (common heterostylous buckwheat) and F. homotropicum Ohnishi (homostylous buckwheat). The percentage of fertilized ovaries of F. esculentum flowers (thrum-type) pollinated with F. homotropicum was 50%, while that of the reciprocal cross was as low as 10% and their ovaries were smaller. Vigorous pollen tube elongation following the bud pollination indicated the possibility for overcoming of breeding barriers in interspecific crosses between buckwheat species. Pre-embryo formation was observed 48 hours after pollination and ovules cultured up to 5 days after pollination tended to develop callus. But ovules isolated 11 days after pollination had apparently lost their viability. Hybrid plantlets were produced from only the ovules dissected 5 days after bud pollination. Regeneration rate was better in ovule culture on White medium than other mediums tested. Interspecific hybrid plants between F. esculentum and F. homotropicum were regenerated with the rate of 24.2% in average.

보정 프로그램을 이용한 Plastic 렌즈 Core의 보정에 관한 연구

우선희(Sun-Hee Woo),이동주(Dong-Joo Lee) 한국생산제조학회 2008 한국생산제조학회지 Vol.17 No.4

In order to meet the optical performance in the process of the micro lens manufacturing with plastics, it is important to embody accuracy in shape and surface roughness to the intended design. Since it is difficult to machine exactly the mold core of lens fit to the designed shape, in this paper, a simple program using MATLAB is developed for shape correction of the mold core after first machining it. This program evaluates correction parameters(aspheric coefficients and curvature) and generates aspheric NC data for compensating the core surface in prior machining process. The program provides the way to manufacture plastic injection molding lens with aspheric shape of high precision, and is expected to be effective for correction and to shorten the processing time.

노인요양시설 조력자 수에 따른 피난소요시간 예측

우선희(Sun-Hee Woo),최준호(Jun-Ho Choi) 한국화재소방학회 2022 한국화재소방학회 학술대회 논문집 Vol.2022 No.춘계

현재 우리나라는 인구 고령화가 진행되고 있으며, 이에 따라 노인요양시설의 수도 증가하고 있다. 노인요양시설에는 이동능력이 비교적 낮은 노인들의 재실 비율이 높기 때문에 다른 용도의 건물에 비해 피난계획 수립이 까다로워야 한다. (Huang, 2019) 특히, 자력 피난이 불가능한 침상(와상)환자도 존재하기 때문에 조력자의 적정 수를 계산하는 것이 매우 중요하다. (Kim, 2021) 이에 본 연구에서는 Pathfinder 피난 시뮬레이션 모델링을 통해 조력자의 수와 자력피난이 불가능한 환자의 비율을 바꿔가며 피난소요시간(RSET)을 예측, 분석하였다. 피난시뮬레이션 시나리오 구성을 위해 자력 피난이 가능한 환자와 불가능한 환자의 비율을 기준으로 총 6개의 케이스로 분류하였다. 또한, 조력자 수를 변수로 설정하고 「노인복지법」에 의거하여 최소 2명에서 최대 48명까지 총 24개 케이스로 구분하였다. 즉, 자력피난이 가능한 환자의 비율과 조력자의 수에 따라 피난시뮬레이션 시나리오를 총 144개로 분류하고 Pathfinder 프로그램을 구동하여 RSET을 예측하였다. 이 때, 재실자의 위치나 반응시간(Pre-evacuation time)은 모두 동일하게 입력하여 시나리오가 바뀌더라도 이들에 대한 오차는 발생하지 않도록 설정하였다. 피난 시뮬레이션을 통해 계산된 RSET을 통계학적으로 분석한 결과, 자력 피난이 불가능한 환자의 재실비율과 조력자의 수에 따라 RSET에 유의미한 차이가 발생하는 것으로 나타났다. (p<.05) 그리고 자력 피난이 불가능한 환자의 비율이 늘어날수록 RSET은 증가하고, 조력자 수가 늘어남에 따라 RSET이 감소하는 패턴을 확인하였다.

Dual Pickup 대물렌즈의 생산을 위한 주요 Parameter 및 특성에 관한 연구

우선희(Sun-Hee Woo),이동주(Dong-Ju Lee) 한국생산제조학회 2007 한국생산제조학회지 Vol.16 No.3

In order to operate CD and DVD compatibly in a pickup system, the objective lens comprise diffractive optical element(DOE) zone and aspheric curvature on its lens surface. The DOE objective lens is effective to simplify this dual-purpose pickup system of the 655㎚ and 785㎚ wavelength by using only one lens, but requires more precision manufacturing process and system due to the complicated shape. This paper presents the overall manufacturing process of this objective lens and describes main parameters in each process, for the correction of the aspheric surface in its core, the shrinkage compensation after injection molding, and the uniformity compensation by adjusting molding conditions.

전단 자극에 의한 심방 근세포 칼슘 웨이브의 발생 : Phospholipase C-이노시톨 1,4,5-삼인산 수용체 신호전달의 역할

김준철(Joon-Chul Kim),우선희(Sun-Hee Woo) 大韓藥學會 2015 약학회지 Vol.59 No.4

Cardiac myocytes are subjected to fluid shear stress during each contraction and relaxation. Under pathological conditions, such as valve disease, heart failure or hypertension, shear stress in cardiac chamber increases due to high blood volume and pressure. The shear stress induces proarrhythmic longitudinal global Ca2+ waves in atrial myocytes. In the present study, we further explored underlying cellular mechanism for the shear stress-induced longitudinal global Ca2+ wave in isolated rat atrial myocytes. A shear stress of ~16 dyn/cm2 was applied onto entire single myocyte using pressurized fluid puffing. Confocal Ca2+ imaging was performed to measure local and global Ca2+ signals. Shear stress elicited longitudinally propagating global Ca2+ wave (~80 μm/s). The occurrence of shear stress-induced atrial Ca2+ wave was eliminated by the inhibition of ryanodine receptors (RyRs) or inositol 1,4,5-trisphosphate receptors (IP3Rs). In addition, pretreatment of phospholipase C (PLC) inhibitor U73122, but not its inactive analogue U73343, abolished the generation of longitudinal Ca2+ wave under shear stress. Our data suggest that shear-induced longitudinal Ca2+ wave may be induced by Ca2+-induced Ca2+ release through the RyRs which is triggered by PLC-IP3R signaling in atrial myocytes.

Dual Pickup 대물렌즈의 생산을 위한 주요 Parameter 및 특성에 관한 연구

우선희(Sun-Hee Woo),이동주(Dong-Ju Lee) 한국생산제조학회 2006 한국공작기계학회 춘계학술대회논문집 Vol.2006 No.-

In order to operate CD and DVD compatibly in a pickup system, the objective lens comprise diffractive optical element(DOE) zone and aspheric curvature on its lens surface. The DOE objective lens is effective to simplify this dual-purpose pickup system of the 655㎚ and 785㎚ wavelength by using only one lens, but require more precision manufacturing process and system due to the complicated shape. This paper presents the overall manufacturing process of this objective lens and describes main parameters in each process, for the correction of the aspheric surface in its core, the shrinkage compensation after injection molding, and the uniformity compensation by adjusting molding conditions.