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Lipopolysaccharide로 유도된 BV-2 세포주의 염증반응에 대한 미세조류 부산물 당 가수분해물의 저해 활성
엄성환 ( Sung Hwan Eom ),최인후 ( In Hu Choe ),권혁주 ( Hyuck Ju Kwon ),이재화 ( Jae Hwa Lee ),이상훈 ( Sang Hoon Lee ) 한국키틴키토산학회 2015 한국키틴키토산학회지 Vol.20 No.1
In this study, we investigated anti-neuroinflammatory activities of enzymatic extracts of the microalgae residues, which were derived from the lipid extraction process, to improve the economic feasibility of algal biofuel. After lipid extraction, three microalgal residues (Chlorella sp., Nannochloropsis sp., Phaeodactylum tricornutum) were enzymatically hydrolyzed by four carbohydrases (Celluclast 1.5 L, Dextrozyme DX 1.5X, Promozyme D2, and Viscozyme L). Antioxidative activities of various enzymatic extracts from algal residues evaluated by measuring 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and alkyl radical-scavenging activities. All enzymatic extracts had scavenging potencies to ABTS + and alkyl radicals. Among the enzymatic extracts, Celluclast 1.5 L extracts from Nannochloropsis sp. residues (CN) showed the highest inhibitory activities on LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in murine BV-2 microglia, and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dosedependentmanner. CN also significantly reduced the generation of proinflammatory cytokines, such as interleukin-6 (IL-6), interleukin- 1β (IL-1β), and tumor necrosis factor (TNF)-α in a dose-dependent manner. Hence, these antioxidant and anti-neuroinflammatory effects of CN might assist potential of microalgae residues as nutraceuticals for treatment and prevention against neurodegenerative diseases that are accompanied by microglial activation.
유산균 발효에 의한 톳 (Hizikia fusiforme) 추출액의 항산화 및 항염증 활성 증가
송호수 ( Ho Su Song ),엄성환 ( Sung Hwan Eom ),강영미 ( Young Mi Kang ),최종덕 ( Jong Duck Choi ),김영목 ( Young Mog Kim ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.2
We previously reported that fermentation by Lactobacillus brevis LB-20 isolated from Kimchi resulted in improvement of the sensory quality of Hizikia fusiforme water extract. This study was conducted to evaluate the possible application of lactic acid bacteria fermentation to improve the functional qualities of H. fusiforme extract. L. brevis LB-20 was inoculated and cultivated in H. fusiforme extract. The antioxidant and anti-inflammatory activities of extract were then assayed both before and following fermentation for two days. Antioxidant activity was determined by assaying levels of radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide, and alkyl radical. Lactic acid bacterial fermentation of H. fusiforme extract resulted in enhancement of antioxidant activity. The greatest enhancement of antioxidant activity was seen in the hydroxyl radical scavenging assay that incorporated 0.5 mg/mL of raw and fermented H. fusiforme extract. Fermented extract exhibited greater (21.95%) inhibition of nitric oxide synthesis than did raw extract (14.66%) at a concentration of 1 mg/mL. The fermented extract exerted its potent anti-inflammatory activity via attenuation of expression of inflammation-related cytokine proteins (TNF-α and iNOS).
대황( Eisenia bicyclis) 추출액의 항산화 및 항염증 활성에 대한 유산균 발효의 영향
한해나 ( Hae Na Han ),엄성환 ( Sung Hwan Eom ),김지훈 ( Ji Hoon Kim ),김덕훈 ( Deok Hoon Kim ),김송희 ( Song Hee Kim ),김윤혜 ( Yunhye Kim ),염승목 ( Seung Mok Yeom ),김영목 ( Young Mog Kim ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.2
This study was conducted to evaluate the effect of lactic acid bacterial fermentation on the antioxidant and antiinflammatory activity of an edible brown alga, Eisenia bicyclis. Lactic acid bacteria were inoculated into and cultivated in E. bicyclis water extract. The antioxidant activity of the extract was assayed before and following fermentation. Antioxidant activity was determined by assaying the levels of radical scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and alkyl radical. The lactic acid bacterial fermentation of E. bicyclis extract resulted in enhanced antioxidant activity. The greatest enhancement of antioxidant activity was seen in the DPPH radical scavenging assay, in which E. bicyclis extract was fermented by Pediococcus pentosaceus MBP-34 strain for 12 h. This fermented extract also exhibited higher inhibitory activity (96.66%) on nitric oxide production compared with other lactic acid bacterial fermented extracts or raw extract (189.60%). In conclusion, fermentation by bacterial strain is an attractive strategy for developing value-added food ingredients.
여드름균(Cutibacterium acnes)에 대한 해조류 추출물의 항균효과
이명석 ( Myeong Seok Lee ),임미진 ( Mi-jin Yim ),이정민 ( Jeong Min Lee ),이대성 ( Dae-sung Lee ),김영목 ( Mog-young Kim ),엄성환 ( Sung-hwan Eom ) 한국수산과학회(구 한국수산학회) 2021 한국수산과학회지 Vol.54 No.1
The study was aimed at developing an alternative therapeutic agent against acne vulgaris, one of the most common skin diseases, to meet the continuing demand for new therapies. Acne vulgaris is often associated with the acne-causing bacteria such as Cutibacterium acnes. To investigate the safety of agents against acne vulgaris, we evaluated the potential antibacterial activities of edible seaweeds against C. acnes in Korea. Forty-one edible seaweeds, including the brown, green, and red varieties, were selected for the antibacterial test. In comparison with other seaweeds, 70% ethanolic extracts of brown seaweeds, such as Cladophora wrightiana var. minor, Eisenia bicyclis, Ecklonia cava, Ishige foliacea, Ishige okamurae, Sargassum filicinum, and Sargassum miyabei Yendo, exhibited potential antibacterial activity against C. acnes with minimum inhibitory concentrations ranging between 64 and 128 μg/mL. To investigate the active anti-acne agents and to enhance our understanding of the antibacterial activities against C. acnes, further solvent-fractionation experiments are warranted. The findings imply that brown seaweeds can be a potential source of natural agents against acne vulgaris.
물김(Pyropia sp.)의 생산 시기별 및 지역별 주요 식품 성분 변화 분석
강민균 ( Min-gyun Kang ),정민철 ( Min-chul Jeong ),박슬기 ( Seul-ki Park ),이장원 ( Jang-won Lee ),조정형 ( Jeung-hyung Cho ),엄성환 ( Sung-hwan Eom ),허만규 ( Man-kyu Huh ),김영목 ( Young-mog Kim ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.5
The study investigated the seasonal and regional changes in the food components of raw laver Pyropia sp.. Samples were obtained monthly from December 2017 to March 2018 at auction markets located in Gangseo-gu, Busan (Nakdong-raw laver), Seocheon-gun, Chungnam, Wando-gun, Jeonnam and Goheung-gun, Jeonnam. The general components, heavy metals and minerals, free amino acids, total phenolic compounds (TPC), and antioxidant activities of the samples were analyzed. No significant physicochemical changes in the moisture, crude lipid, and ash contents were observed in the raw lavers tested. However, the crude protein content of Nakdong-raw laver was the highest in December 2017 at 7.20±0.28% and the content was higher seasonally and regionally than those of the other raw lavers. In addition, the taurine, zinc, manganese and TPC contents of Nakdong-raw laver was higher seasonally and regionally than those of the other raw lavers. The ethanolic extract of Nakdong-raw laver also had higher antioxidant activity, including as DPPH (2,2'-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis [3-ethylbenzothiazoline- 6-sulphonic acid]) radical scavenging activity, although there was no significant correlation between TPC and the antioxidant activity. This study is the first analysis of seasonal and regional changes in the major food components of raw laver cultivated in Korea.
유산균으로 발효한 다시마(Saccharina japonica) 추출물 첨가 명란젓의 품질 특성
황지영 ( Ji-young Hwang ),장종수 ( Jong-soo Jang ),류대규 ( Dae-gyu Ryu ),김경태 ( Kyung Tae Kim ),허만규 ( Man Kyu Huh ),엄성환 ( Sung-hwan Eom ) 한국수산과학회(구 한국수산학회) 2019 한국수산과학회지 Vol.52 No.3
Natural seasoning are trends of food industries to replace synthesized seasoning due to consumer preference. However, unexpected results can be happened in a point of sensory evaluation and physico-chemical properties by adding natural seasoning such as reduced flavor, color change and so on. Therefore, this study aimed to verify the potential of sea tangle Saccharina japonica extract fermented by lactic acid bacteria (STE) for myungran (pollock roe)-jeot instead of present synthesized seasoning. Among various STE concentration (0%, 5%, 10%, and 15%) in myungran-jeot, there was no significant physicochemical changes in a point of crude lipid, pH, total acidity, salinity and color difference. Microbiological profiles was also not significantly different. The 10% STE-myungran-jeot showed higher γ-aminobutyric acid (GABA) content as 24.4 mg/100g at free amino acid analysis than other STE-myungran-jeot without decline of sensory properties. In overall, it revealed that STE could replace the synthesized seasoning and improve the quality of Myungran-jeot. The results suggest that STE can be useful to develop a Korean traditional fermented food.
ICR 마우스를 이용한 Bacillus subtilis JNS 균주의 단회경구투여 독성시험
김경훈(Kyoung-Hoon Kim),정창화(Chang Hwa Jeong),주성제(Seong-Je Joo),박종훈(Jong-Hoon Park),문지영(Ji-Young Moon),조은지(Eun-Ji Cho),이현태(Hyun-Tai Lee),권현주(Hyun-Ju Kwon),김병우(Byung-Woo Kim),엄성환(Sung-Hwan Eom),이은우(Eun-Woo 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.1
청국장에서 분리한 항균활성이 우수한 Bacillus subtilis JNS의 probiotics로서의 활용성을 검증하기 위해 단기투여에 의한 안전성을 확인하고자 실험을 실시하였다. 경구투여용 존데를 이용하여 임상적용 경로인 경구로 투여하였으며, 최고투여용량은 2,000 mg/kg으로 설정하였다. ICR계 암수마우스에서 14일간 관찰한 결과 일반증상, 사망률, 체중, 임상증상 및 육안적 소견 등에서 무처리 대조군과 비교해서 유의성 있는 차이를 보이지 않았다. 부검 후의 주요 장기에 대한 육안적 검사에서 또한 대조군과 비교하여 이상소견이나 병변이 관찰되지 않았다. 이상의 결과로부터 시험물질인 B. subtilis JNS는 경구투여 시 ICR계 암수 마우스에서 독성학적인 변화가 관찰되지 않았으며, 경구투여가 2,000 mg/kg 이상인 저독성의 안전한 물질로 사료된다. The present study was carried out to investigate the in vivo single-dose acute toxicity of Bacillus subtilis JNS isolated from Cheonggukjang, which is a probiotic candidate showing strong and broad antibacterial activity. The test sample was orally administrated to male and female ICR mice at a highest dose of 2,000 mg/kg for 14 days. No significant change in general conditions, mortalities, body weight changes, clinical signs, autopsy findings, or presence of gross lesions was observed in either sex of mice. The results indicate that up to 2,000 mg/kg of B. subtilis JNS had no adverse effect on ICR mice.