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Lipopolysaccharide로 유도된 BV-2 세포주의 염증반응에 대한 미세조류 부산물 당 가수분해물의 저해 활성
엄성환 ( Sung Hwan Eom ),최인후 ( In Hu Choe ),권혁주 ( Hyuck Ju Kwon ),이재화 ( Jae Hwa Lee ),이상훈 ( Sang Hoon Lee ) 한국키틴키토산학회 2015 한국키틴키토산학회지 Vol.20 No.1
In this study, we investigated anti-neuroinflammatory activities of enzymatic extracts of the microalgae residues, which were derived from the lipid extraction process, to improve the economic feasibility of algal biofuel. After lipid extraction, three microalgal residues (Chlorella sp., Nannochloropsis sp., Phaeodactylum tricornutum) were enzymatically hydrolyzed by four carbohydrases (Celluclast 1.5 L, Dextrozyme DX 1.5X, Promozyme D2, and Viscozyme L). Antioxidative activities of various enzymatic extracts from algal residues evaluated by measuring 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and alkyl radical-scavenging activities. All enzymatic extracts had scavenging potencies to ABTS + and alkyl radicals. Among the enzymatic extracts, Celluclast 1.5 L extracts from Nannochloropsis sp. residues (CN) showed the highest inhibitory activities on LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in murine BV-2 microglia, and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dosedependentmanner. CN also significantly reduced the generation of proinflammatory cytokines, such as interleukin-6 (IL-6), interleukin- 1β (IL-1β), and tumor necrosis factor (TNF)-α in a dose-dependent manner. Hence, these antioxidant and anti-neuroinflammatory effects of CN might assist potential of microalgae residues as nutraceuticals for treatment and prevention against neurodegenerative diseases that are accompanied by microglial activation.