히르슈슈프룽병 환자에서 미성숙 신경절 세포의 빈도 및 그 의의

양희범,김현영,김수홍,정성은,박귀원,Yang, Hee-Beom,Kim, Hyun-Young,Kim, Soo-Hong,Jung, Sung-Eun,Park, Kwi-Won 대한소아외과학회 2013 소아외과 Vol.19 No.2

Immature ganglion cell (IGC) is known for its relationship with intestinal motility and its impact on postoperative functional outcomes of Hirschsprung's disease (HD). There are few studies on the relationship between intestinal dysmotility and IGC in HD patients. 67 patients pathologically diagnosed with HD and who received definitive operation in Seoul National University Children's Hospital from 2010 to 2011 were included. 10 patients were excluded due to inadequate immunohistochemical staining results. The proximal end of resected ganglionic segment was evaluated with immunohistochemistry examination with MAP-2, a marker of ganglionic cells and bcl-2, a marker of IGCs The median age at operation was 155 (15-4678) day-old. 55 (96.5%) patients positive for bcl-2, were regarded as having IGC, and 2 (3.5%) patients positive for MAP-2 but negative for bcl-2, were regarded as having only mature ganglion cells. In the bcl-2 positive group, there were 7 patients (12.7%) with constipation, 15 patients (27.3%) with soiling, 3 patients (5.5%) with perianal excoriation and 6 patients (10.9%) with medication use. In bcl-2 negative group, intestinal dysmotility was not seen. There was no statistical significance in the two groups. Considering that HD is diagnosed at a young age, the rate of IGC present is very high and it might be inappropriate to relate IGC to functional outcome at young ages.

차 종자 저장 및 파종 처리가 종자의 발아에 미치는 영향

양희범(Hee-Beom Yang),이성철(Seong-Cheol Lee),김세림(Se-Lim Kim),이민석(Min-Suk Lee),이진호(Jin-Ho Lee),김영걸(Young-Gul Kim),송관정(Kwan-Jeong Song) 한국차학회 2008 한국차학회지 Vol.14 No.2

본 연구는 차나무 교배육종 기술체계를 확립할 목적으로 차나무 교배 실생의 획득률을 높일 수 있는 기초 자료를 얻고자 수행하였으며, 차나무에서 채종된 종자의 발아온도, 저온처리 기간, 종피 처리, 침윤 기간, 저장 방법 등 몇 가지 요인이 차 종자 발아에 미치는 영향을 분석하였다. 발아온도는 Saemidori, Topyung 및 Okumidori 3품종 모두 25℃에서 발아율이 가장 높게 나타났다. 그리고 저온처리의 영향은 Saemidori와 Okumidori 두 품종 종자 모두 저온처리 기간 50일, 63일, 75일이 38일보다 발아율과 발아속도가 높게 나타났다. 자연 건조된 Topyung 종자의 경우 종피 제거처리가 발아율이 64%로 종피 부분적 파쇄와 무 처리구보다 훨씬 높았고 Saemidori 종자의 종피 제거 처리구의 최종 발아율은 83.3%로 무 처리구의 47.5%보다 높게 나타났다. 침윤 처리 실험에서 Yabukita 종자의 최종 발아율은 침윤 2, 3, 4일 후 파종된 종자가 높았고 Okumidori 종자는 침지 3, 4일 후 파종된 종자가 높았다. 종자 보관 방법에 있어서 Yabukita 종자는 습윤 보관이 건조 보관한 종자보다 발아율이 높았고 Topyung, Okumidori 종자는 두 처리구들 간에 차이가 없었다. 발아속도는 3품종의 종자에서 모두 습윤 보관의 경우 높게 나타났고 종자 생체중이 증가되었다. The study was conducted to obtain fundamental data related to efficient increase of the seedling population aiming to establish an efficient cross breeding system for tea plants. Several parameters affecting seed germination, such as germination temperature, chilling duration, seed coat treatment, imbibition duration, and storage method, were evaluated. Of germination temperature, the highest germination percentages were obtained at 25℃ for all three varieties of Saemidori, Topyung, and Okumidori. Chilling duration of 50, 63, and 75 days had higher germination percentages and speeds of germination than those of 38 days in both varieties of Saemidori and Okumidori. Final germination percentage by seed coat removal in Topyung dried naturally showed 64 %, much higher than those by partial seed coat crack and control, and that for Saemidori, 83.3%, higher than 47.5% for control. Imbibition duration of 2, 3, and 4 days in Yabukita and those of 3 and 4 days in Okumidori showed higher germination percentages than the others. Wetness of seed storage method indicated higher germination percentage than dryness in Yabukita, however there were no differences in germination percentages of Topyung and Okumidori by storage method. On the other hand, speed of germination was higher at wetness for all three varieties in accordance with increase of seed fresh weight.

차나무 인공교배에 의한 수정 및 종자 형성

양희범(Hee-Beom Yang),이성철(Seong-Cheol Lee),김세림(Se-Lim Kim),이민석(Min-Suk Lee),이진호(Jin-Ho Lee),김태춘(Tae-Choon Kim),김영걸(Young-Gul Kim),송관정(Kwan-Jeong Song) 한국차학회 2008 한국차학회지 Vol.14 No.2

차에서 효율적인 교배육종 시스템을 확립코자 몇 가지 교배조합에서의 연차 간 결실율과 종자 형성율을 평가하였다. 10% 자당, 2% 한천, pH 5.6의 화분 발아 배지에서의 화분 발아 검정은 인공교배에 이용한 화분의 활력이 정상적이었음을 나타냈다. 인공교배 3일 후 이내에 화분관은 화주를 통해 자방의 상단을 침입하는 것으로 나타났다. 교배조합에 따른 결실율은 3.7~57.7%, 열매 당 종자 수는 1.4~3.0 개 내외를 나타냈다. 연차 간 변이에 있어서 열매 당 종자 수의 변이는 미미했고, 결실율의 변이는 다소 크게 나타났다. 자방은 3개의 심실로 구성되고 심실 당 4개의 배주가 위치하여 총 12개 배주가 형성됨을 확인하였다. Aiming to establish an efficient conventional breeding system in tea (Camellia sinensis), fruit set and seed formation with seasonal variation were evaluated at some cross combinations. The pollen germination test was conducted on a medium consisting of 10% sucrose, 2% agar, pH 5.6 and the result indicated that pollens used in artificial pollination had normal germination activity. Pollen tubes penetrated through style into ovary within 3 days after artificial pollination. Fruit set was ranged at 3.7~57.7% and average number of seeds per fruit was varied at 1.4~3.0. Amount of variation was little at average number of seeds per fruit, but much high at fruit set. Morphological analysis determined that one ovary had three carpels and 12 ovules, including 4 ovules a one carpel, respectively.

양자장론을 통한 입자 스핀의 이해

양희범 ( Hee Beom Yang ),신창섭 ( Chang Sub Shin ) 충남대학교 기초과학연구원 2023 충남과학연구지 Vol.40 No.1

In this study, we aim to gain a more detailed understanding of particle spin using the tools of quantum field theory. Firstly, we delve into the fine structure of the hydrogen atom, investigating the discrepancies between the energy levels predicted by the Schrödinger equation and experimental results. These variations are attributed to various interactions, including relativistic effects and spin-orbit coupling. Our emphasis lies particularly on the impact of spin-orbit coupling, exploring the influence of the interaction between the electron's spin, manifested as a magnetic dipole moment, and the potential of the atomic nucleus on energy level changes. Secondly, we focus on the classification of particles into fermions or bosons based on whether their spin is a integer or a half-integer. As each classification leads to different exchange behaviors of particles, we investigate their responses accordingly and derive the spin-statistic theorem.

수박계통간 염색체수준의 유전적변이 분석

김윤성,고찬섭,양희범,강순철,Kim, Youn-Sung,Ko, Chan-Sup,Yang, Hee-Beom,Kang, Sun-Chul 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.2

수박의 형태적 변이의 유전적 원인을 분석해 보기 위해 8개 계통에서 re-sequencing을 수행하였다. 유전적 변이의 수는 염색체에 따라 다르게 나왔으며 발견된 SNP의 약 12.9%만이 유전자내에서 발견되었고 나머지는 프로모터나 유전자 사이의 지역에서 발견되었다. SNP 밀도에 대한 분석 결과 염색체 6번의 말단지역에 변이가 집중되어 있는 것을 알 수 있었다. 또한 염색체 10과 11번에 잘 보존된 지역을 발견하였다. Pathway 분석을 통해 DIMBOA(일종의 항생제)-glucoside 분해 대사가 계통간 가장 차이나는 것으로 확인되었으며 이는 각 계통의 병저항성에서 차이가날 가능성을 시사하는 것이다. 당대사 관련 유전자 변이를 분석한 결과 alpha-galactosidase 유전자에 가장 변이가 많은 것으로 밝혀졌다. 이러한 연구 결과는 육종을 분자수준에서 이해하는 데 도움을 줄 것으로 생각한다. To investigate the genetic basis of phenotypic differences, sequence variations were analyzed in 8 inbred watermelon lines by re-sequencing. The number of sequence variations differed depending on the chromosome. Only 12.9% of SNPs were found within genes, whereas the rest were detected in promoter or intergenic regions. SNP density analysis showed that there was a highly variable region at the end of chromosome 6, which is similar to previously published findings. However, this region with high SNP density did not show much variation between the lines. In contrast, highly conserved regions with a size of 6.5-10 Mb were found in chromosomes 10 and 11. Pathway analysis suggested that the DIMBOA (a natural antibiotic)-glucoside degradation pathway was significantly different between the lines, indicating that the eight lines may have different levels of pathogen resistance. Among the carbohydrate-related genes, the alpha-galactosidase gene was the most variable among the lines. Information from this study will be helpful in understanding the watermelon breeding process at the molecular level.

딥러닝 이미지 분석을 통한 당뇨병 환자 식이요법 서포팅 서비스 개발

서시현(Si-hyun Seo),양희범(Hee-beom Yang),김기융(Ki-yung Kim),박현준(Hyun-jun Park) 한국정보통신학회 2022 한국정보통신학회 종합학술대회 논문집 Vol.26 No.2