김(Porphyra sp.)에서 추출한 PDRN (polydeoxyribonucleotide)의 나일틸라피아(Oreochromis niloticus)에서의 상처 치유 효과

양찬영 ( Chan Yeong Yang ),한지성 ( Ji Sung Han ),이원세 ( Won Se Lee ),배준성 ( Jun Sung Bae ),이채원 ( Chae Won Lee ),정은하 ( Eun Ha Jeong ),김관희 ( Gwan Hui Kim ),박관하 ( Kwan Ha Park ) 한국어병학회 2021 한국어병학회지 Vol.34 No.2

Polydeoxyribonucleotide (PDRN)은 특정 adenosine 수용체에 작용하여 상처 치유 과정을 촉진하는 것으로 알려져 있다. 지금까지 어류의 조직에서 얻어진 PDRN과는 다르게, 최근 해초류인 Porphyra로부터 추출된 PDRN이 개발되었다. 본 연구는 나일틸라피아(Oreochromis niloticus)에서 인공적으로 피부의 상처를 유발한 뒤 Porphyra에서 추출한 PDRN을 이용하여 상처 치유 효과를 평가하였다. 상처의 회복은 시험 시작 후 1주내에는 일부만 회복되었지만, 2주내에 거의 완전히 회복되었다. 상처를 유발하기 전 PDRN을 10 및 20 mg/kg의 용량으로 5일간 사료로 투여한 후 상처 치유 효과를 관찰하였다. 상처 치유 효과는 육안적, 면적 및 색도 측정으로 평가하였다. 본 연구에서 관찰된 Porphyra 유래의 PDRN에 의한 상처 치유 효과는 다양한 원인에 의해 발생하는 어류의 상처 치유 목적으로의 활용 가능성을 보여준다. Polydeoxyribonucleotide (PDRN) is known to accelerate wound healing process by acting on specific adenosine receptors. Differently from the PDRN substances obtained so far from piscine tissues, unique PDRN was prepared recently from Porphyra, a seaweed species. In this study, effects of the Porphyra PDRN was evaluated by inducing artificial skin wounds in Nile tilapia Oreochromis niloticus. The wound recovered almost completely in 2 weeks without any treatment, however only a partial recovery was done after one week. Facilitated healing was observed by PDRN fed for 5 days before wound induction at 10 or 20 mg/kg, against the partially recovered 1-week old wound. The healing effects were identified not only by visual observation, wound area measurement, but also by colorimetric estimations. These novel wound healing effects suggest beneficial therapeutic utility of Porphyra-derived PDRN for wound treatments in fishes occurring from various causes.

흰다리새우(Litopenaeus vannamei)에서 광범위 반합성 항생제 doxycycline의 독성연구

이채원 ( Chae Won Lee ),배준성 ( Jun Sung Bae ),양찬영 ( Chan Yeong Yang ),정은하 ( Eun Ha Jeoung ),이지훈 ( Ji-hoon Lee ),박관하 ( Kwan Ha Park ) 한국어병학회 2020 한국어병학회지 Vol.33 No.1

Doxycycline is a semi-synthetic broad-spectrum antibiotic, and it has been used to get rid of bacteria in animals and humans. The use of antibiotics has greatly contributed to the aquaculture production although its misuse sometimes presents public health problems. This study was performed to investigate the toxic effects of doxycycline on whiteleg shrimp (Litopenaeus vannamei) administered for possible infection treatments. The shrimp were allocated into four groups and doxycycline was fed three times a day for 7 days at 0, 20, 50 and 100 mg/kg to each group. After 24 hr following the 7-day treatment, hemolymph and hepatopancreas were used for blood and biochemical analysis: Total hemocyte counts, Total protein, Total cholesterol, Gluscose, Glutamic pyruvic transaminase, Glutamic oxaloacetic transaminase, Glutathione peroxidase, Superoxide dismutase, Glutathione-s-transferases, Total antioxidant capacity colorimetric and Acid phosphatase. In addition, histopathological examination was performed on the hepatopancreas and muscle. It was observed that body weight gain was significantly retarded in 100 mg/kg doxycycline group. Doxycycline was found to induce biochemical or functional disorders at 100 mg/kg as observed many of the blood and biochemical parameters were significantly reduced. In conclusion, it was judged that there will be no major toxicity problems with doxycycline when used for shrimp aquaculture at regular doses.

저염도에서 생육가능한 미생물 probiotics가 저염분 양식의 흰다리새우(Litopenaeus vannamei)의 성장 및 면역능에 미치는 영향

배준성 ( Jun Sung Bae ),이채원 ( Chae Won Lee ),양찬영 ( Chan Yeong Yang ),정은하 ( Eun Ha Jeong ),김은진 ( Eun-jin Kim ),박관하 ( Kwan Ha Park ) 한국어병학회 2020 한국어병학회지 Vol.33 No.2

흰다리새우는 광범위한 염도(1-40 psu)에서 생존 가능하다. 하지만 염도의 변화, 특히 낮은 염도(1-5 psu)는 생존율, 비특이적 면역능 및 항병능과 같은 다양한 생리적 변화에 영향을 미칠 수 있다. 본 연구에서는 73일 동안 경구 투여 및 사육수 첨가를 동시에 수행한 뒤 성장, 비특이적 면역 및 질병 저항성을 측정하였다. 사육수의 염도는 주기적으로 소금을 첨가하여 3 psu를 유지하였다. 그 결과 73일간 사육 후 생존율이 크게 증가하였으며 (5.6 vs. 15.4%), reactive oxygen species (ROS) 생산과 phenol oxidase (PO) 활성의 유의성있는 증가가 확인되었다. 대조적으로 superoxide dismutase (SOD)활성은 영향을 받지 않았다. 새우에서 Vibrio alginolyticus의 인위 감염 시험의 결과, 프로바이오틱스 처치구에서 약간의 폐사 감소가 관찰되었다 (100 vs. 79%). 결과적으로 저염도(3 psu)에서 양식된 흰다리새우의 생산은 저염도에서 생존가능한 프로바이오틱스에 의해 증가 될 수 있을 것이다. The whiteleg shrimp Litopenaeus vannamei can survive in a wide range of salinity (1-40 psu). However, such variation, particularly at low salinity (1-5 psu), can affect various physiological changes such as survival rates, non-specific immunity and disease resistance. In this study, growth, non-specific immunity and disease resistance were measured following simultaneous oral feeding and addition of probiotic microbes into culture water for 73 day period. The salinity of the culture water was maintained at 3 psu by periodical salt additions. The result shows that survival rate increased significantly (5.6 vs. 15.4%) after 73 day rearing. Significant increases were identified in reactive oxygen species (ROS) production and phenol oxidase (PO) activity. However, superoxide dismutase (SOD) activity was not influenced. When the shrimp was artificially challenged with Vibrio alginolyticus, slight mortality reduction was observed in the probiotics-treated group (100 vs. 79%). In conclusion, the production of cultured whiteleg shrimp at low salinity might be increased by probiotics survivable at low salinity levels.

커뮤니티 비즈니스의 중장기 육성전략(3/3차연도)

김태곤(Tae-Gon Kim),김정섭(Jeong-Seop Kim),허주녕(Joo-Nyung Heo),양찬영(Chan-Yeong Yang) 한국농촌경제연구원 2013 한국농촌경제연구원 기본연구보고서 Vol.- No.-

Background of Research Several significant factors such as market opening followed by globalization, regulations relaxation, decreasing population, and aging society encourage the lower growth of agriculture and commercial industry in rural areas, as well as induce the collapse of community. Both decline and growing aspects are shown in a typical rural area, and the polarization has become more intensified. Current policy and administrative service are limited to apply and response to various and complicated regional issues including income disparity and the collapse of community. Recently, as a new form of business based on regional unit or community has emerged, a higher and significant need for improving response strategy focused on small scale or site-contact is magnified. Method of Research As a key method in this study, the outstanding examples or cases were discovered and dissemination potential was verified through the fact finding survey. Case study and applicability are to be reviewed through the case study of regional vitalization overseas. Especially, ‘site discussion forum’ and ‘community business study meeting’ were held to draw site-focused study results. Regarding the specific expertise area, its related information has been collected through commissioned researches and an international symposium. Research Results and Implications Therefore, the purpose of this study is to find out the role and actual condition of community business (CB) and analyze the ripple effect to suggest the promotion strategy of community business. This study aims to: ① create regional level jobs for a short term to improve the added value, ultimately, vitalizing a declined economy in rural area; ② solve social issues intensified in rural areas for a mid and long term to ultimately ‘improve the quality of life’ and ‘realize the balanced society’. Key table of contents for this study includes: ‘concept and role of community’, ‘type and case of community businesses’, ‘policy related to community businesses’, ‘promotion of community business project’, ‘increasing demand of social service and community business’, ‘community restoration and urban-rural exchange of urban agriculture’, ‘promotion strategy of community business’, etc. This study is a final report that combines the results of project undertaken over 3 years. The policy precedes the research for community business and this community business is dispersed and operated based on the needs by governmental department. The community business is divided into 4 areas in this study: agri-business, resource environment, living welfare and urban- rural exchange. This study is differentiated from other existing studies in that it aims to resolve economic problems, properly response to increasing social demands including living and welfare followed by ageing society and ultimately, realize the balanced society.

구피(Poecilia reticulata)에서 수산용의약품 oxolinic acid, neomycin-oxytetracycline, florfenicol의 급성독성 및 Aeromonas salmonicida에 대한 약효 평가

배준성 ( Jun Sung Bae ),이채원 ( Chae Won Lee ),양찬영 ( Chan Yeong Yang ),정은하 ( Eun Ha Jeong ),김아름 ( Areum Kim ),채영식 ( Young-sik Chae ),박정진 ( Jung-jin Park ),박관하 ( Kwan Ha Park ) 한국어병학회 2023 한국어병학회지 Vol.36 No.2

This study evaluated the acute toxicity induced by oxolinic acid (OA), neomycin-oxytetracycline combination (NEO-OTC) and florfenicol (FF) administered to guppy (Poecilia reticulata) by comparing standard formulations with commercial preparations (COOaqua curinpotion, COOaqua terafinpotion and COOaqua profenpotion, respectively) for ornamental fish at concentrations of 2-4%. NEO-OTC exhibited the highest acute toxicity in guppy, with no difference observed between the pstandard formulation and commercial preparation (LC₅₀ = 126.08 mg/L and 112.44 mg/L, respectively). OA acute toxicity was assessed in the form of sodium salt, with an LC₅₀ of 504.61 mg/L for the standard formulation and a slightly increased toxicity of 316.11 mg/L for the commercial preparation. In contrast, no mortality was observed during the 96-hour exposure period with the standard formulation in the form of oxolinic acid. The acute toxicity of FF was measured to be above 1,000 mg/L for the standard formulation; however, the commercial preparation significantly increased to 158.53 mg/L. These results indicate that toxicity can significantly increase in commercial formulations, especially those with low levels of active ingredients. This is presumed to be attributed to the organic solvents or solubilizing agents present in the commercial preparations, which may enhance toxicity. Additionally, guppy infected with Aeromonas salmonicida were effectively protected against mortality by administering OA, NEO-OTC and FF at concentrations of 50 mg/L, 100 mg/L and 15 mg/L, respectively, for 2 hours and at half the dose for 24 hours. This result indicates that liquid formulations containing low concentrations of antibiotics may partially increase toxicity, but there is no problem in effectively treating diseases in ornamental fish.