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양수인,위인선,이종빈 ( Soo In Yang,In Sun Wui,Jong Bin Lee ) 한국환경생물학회 1993 환경생물 : 환경생물학회지 Vol.11 No.1
Toxic effects of Hg^2+, Cd^2+ and Cr^6+ on developing chick embryos were examined by injecting the metal ions (1, 5, 10 and 50㎍ of each in saline solution) into yolk sac of fertilized egg. The injections were performed just before 3 days or 6 days after incubation. It was investigated on the enzyme activities (LDH, MDH, SDH) in liver, heart and pectoral muscle at 14 days after incubations. 1. The degree of decrease in the activities of LDH and MDH were greater than that of SDH. The relative sensitivity of the enzymes was MDH > LDH > SDH. 2. The toxicity of the metal inos is more heavy at early embryonic stage. The relative toxic effect of the metal inos was Cd^2+>Cr^6+>Hg^2+. 3. The relative sensitivity of tissues on the metal ions was liver > pectoral muscle > heart.
목포시 도시근린공원에서 Well-being을 위한 대기환경 특성
임항선 ( Hang-seon Lim ),이정일 ( Jeong-il Lee ),박찬오 ( Chan-o Park ),박종수 ( Jong-soo Park ),정경모 ( Kung-mo Jung ),양수인 ( Soo-in Yang ) 한국환경기술학회 2015 한국환경기술학회지 Vol.16 No.4
본 연구는 목포시 도시근린공원 입암산과 양을산을 선정하여 피톤치드(11종), 음이온, 미세먼지, 총부유세균, 가스상 성분을 측정하였고, 동시간대 대기오염측정소 결과를 비교 분석하여 숲 기능의 장점을 찾아보고자 하였다. 피톤치드는 숲속에서 측정했던 평균농도는 797.5 ~ 986.4 pptv였고, 2시간 산책하면서 측정했던 평균농도는 382.1 ~ 415.7 pptv로 나타났다. 음이온은 바닷가에 위치 입암산공원이 양을산 보다 더 높게 나타났으며, 도시지역과 공지역이 낮게 나타났다. 가스상 물질인 질소산화물은 출근 시간대인 오전 7~10시에 높게 나타났으며원지역을 비교한 총부유세균과 PM-10은 도시지역에 비해 공원, 오존은 도시지역과 공원지역의 농도 차이가 없었으며 시간대별 오존농도는 정오경에 가장 높은 농도를 나타냈다. The study was carried out to investigate the concentration distribution of phytoncides, PM-10, anion, total suspended bacteria, nitrogen oxide and ozone on the air quality of Yangeulsan and Ibamsan urban-area parks on Mokpo city. In phytoncides, the mean value measured in each site showed 797.5 ~ 986.4 pptv in case of measuring air quality at position fixed in specific spot and 382.1 ~ 415.7 pptv in case of mobile measurement for air quality taking a walk during two hours. In anion, it showed that Ibamsan located near by sea was higher than Yangeulsan located near city`s center. In PM-10 and total suspended bacteria, the concentration measured in two urban-area parks was lower than that of city area. In NOx concentration of gaseous material was highest in the morning rush hours 7 to 10 o`clock. In ozone concenration, there was no difference of air quality between urban area parks and city area, while ozone concentration was the highest value in noon when UV rays were the strongest.
재배지역에 따른 농산물의 phytochemical 함량
나환식 ( Hwan Sik Na ),김진영 ( Jin Young Kim ),윤설희 ( Seol Hee Yun ),박학재 ( Hak Jae Park ),최경철 ( Gyeong Cheol Choi ),양수인 ( Soo In Yang ),이지헌 ( Ji Heon Lee ),조정용 ( Jeong Young Cho ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.4
최근 건강 증진을 위해 소비가 증가하고 있는 농산물을 대상으로 phytochemical 성분을 정량하고, 다양한 phytochemical 성분을 동시에 분석하는 방법에 대해 조사하였다. Lycopene, a, β-carotene, cryptoxanthin과 lutein을 대상으로 동시분석을 실시한 결과 회수율은 각각 lycopene 120.7±4.1%, lutein 89.1±3.5%, a-carotene 91.2±2.9%, β-carotene 99.1±4.4%, cryptoxanthin 100.0±5.3%로 나타나 향후 다양한 phytochemical 성분을 동시에 분석하는데 유효하 방법이 될 것으로 판단된다. 농산물을 대상으로 4종의 phytochemical(β-carotene, lycopene, lutein 및 cryptoxanthin)을 분석한 결과, lutein은 시금치>참다래>토마토>블루베리>메론 순이었으며 시료별 분석 결과 시금치(나주, 신안산), 참다래(해남, 보성산)와 블루베리(담양산)가 타 시료에 비해 luein 함량이 더 높은 결과를 보였다. Lyeopene은 토마토와 시금치에서 검출되었으며, 분석 시료 중 흑 토마토 (56.66±7.48 mg/kg)와 장성 토마토(50.28±5.42 mg/kg)의 lyeopene 함량이 가장 높았다. β-carotene의 경우 시금치와 토마토 시료에 가장 많이 함유되어 있었으며 특히, 나주 시금치(65.03±4.83 mg/kg)와 신안 시금치(37.67±5.49 mg/kg)에 다량 함유되어 있었다. Quercetin 분석 결과 블루베리에서 가장 높게 검출되어 담양산 블루베리가 1.054.06±80.54mg/100g으로 수입산(986.57±67.85 mg/100 g)보다 높았으며, kaempferol의 경우도 비슷한 경향을 보였다. 총 폴리페놀의 경우 블루베리(213.60~229.96 mg/100 g)가 가장 높은 결과를 보였고, 시금치(112.50~141.67 mg/100g), 참다래(46.49~70.44 mg/100g) 에서도 높게 검출되었다. 총 플라보노이드 함량을 분석한 결과 블루베리, 시금치 시료에서 타 시료에 비해 함량이 높게 검출되었으며, 비타민 C 함량은 참다래(39.45~86.79 mg/100 g), 한라봉(38.65~50.96mg/100g), 토마토(5.90~15.97 mg/100g), 블루베리 순으로 나타났고, 블루베리에서 총 안토시아닌 함량을 측정한 결과 담양산 블루베리>수입산 블루베리 순으로 나타났다. 이상의 결과로 보아 유통 중인 농산물에 다양한 phytochemical 성분이 존재하는 것으로 확인되었으며, 이는 재배지역, 품종, 숙성 정도 등에 따라 조금식 차이가 나는 것으로 판단된다. The purpose of this research is to distinguish the quantitative determination of phytochemicals in various agricultural products and to optimize an HPLC method for the determination of Iycopene, lutein, a-carotene, i3-camtene and cryptoxanthin. Among the different conditions studied, the most suitable on for our sampesl were the extraction with hexane/acetone/ethanol (50:25:25 v/v/v) diolution of the dry extract in tetrahydrofuran/acetonitrile/methanol (15:3055, v/v/v) injection on a CIS column with methanol/acetonitrile (90:10 v/v) + trithylamine 9 μM as mobile phase, and □detection=475 nm. The mean percent recovery for the HPLC method were 120.7±4.1 % (Iycopene), 89.2 ±3.5% (lutein), 91.2±2.9% (a-carotene) 99.1±4.4% (β-carotene), and 100.0±5.3% (cryptoxanthin). The contents of lutein in tile agricultural products were spinach, kiwi tomato, blueberry melon, respectively. However. the Iycopene contents were the highest in the Black tomato (56.66±7.48 mg/kg) and Jangseong tomato (50.28±5.42 mg/kg). The concentration of β -carotene in all of tile agricultural products ranged from 0.07 mg/kg to 65.03 mg/kg. The quercetin content of the agricultural products increased in the order of blueberry (986.57 -1 054.06 mg/l00 g), kiwi (44.96~55.09 mg/100 g), hallabong (31.92~35.60 mg/100 g) and tomato (26.38-34.94 mg/100g). The highest kaempferol content was found in tile blueberry (47.79~76.15 mg/100 g) with results in all of the tested samples varying between 6.54-48.11 mg/100 g. The total polyphenol contents of the various agricultural products increased in the blueberry (213.60~229.96 mg/l00 g) spinach (112.50~141.67 mg/100 g) and kiwi (46.49~70.44 mg/100 g). The total flavonoid content was the highest in both blueberry and spinach. Vitamin C content was detected in kiwi > hallabong> tomato> blueberry, respectively. The total anthocyanin contents (TAC was detected in the Damyang blueberry and the imported blueberry.
6가크롬이 계배 간세포의 DNA , RNA , 및 단백질 함량에 미치는 영향
이종빈,양수인,위인선 ( Jong Bin Lee,Soo In Yang,In Sun Wui ) 한국환경생물학회 1988 환경생물 : 환경생물학회지 Vol.6 No.1
Cr^(6+)(1, 5 and 10㎍) was injected into the yolk sac of the fertilized eggs at certain time(0, 3 and 6 days) after incubation. At 14 days after incubation, the effects of Cr^(6+) on the mortality and the contents of the DNA, RNA and protein in surviving chick embryo liver were inverstigated. The other group injected Cr^(6+) at 13 days after incubation, the effects of Cr^(6+) on the contents of the macromolecules in chick embryo liver with the passing of time were investigated. When Cr^(6+) was injected just before incubation, the LD_50 was 10㎍. The longer and more expose to Cr^(6+), the more increeasingly the mortality and the decrease in the contents of the macromolecules. Decrease in the content of RNA was the most remarkable as compare to that of DNA or protein. It is suggest that the toxicity of Cr^(6+) firstly effect the transcriptional process. In the condition Cr^(6+) was injected into eggs which were incubated for 13 days. The contents of the macromolecules of liver after 3 days from injection decreased much more that of 1 day from injection. While, as time passed(after 5 and 7 days from injection) and contents of the macromolecules of liver recovered.