본태성 고혈압 환자의 치료경과에 따른 뇨중 N - Acetyl - β - D - Glucosaminidase 의 활성도

안중기(Joong Ki Ahn),이승호(Seung Ho Lee),하종영(Chong Young Ha),김원호(Won Ho Kim),고재기(Jae Ki Ko) 대한내과학회 1988 대한내과학회지 Vol.34 No.5

N/A The urinary excretion of N-acetyl-p-glucosamidase (NAG) is increased in patients whose renal function is impaired by a variety of kidney disease, and may provide an index of renal injury. To assess its role in essential hypertension, we measured urinary levels of NAG in 44 subjects with essential hypertension (and no evidence of renal disease), 14 subjects with well controlled hypertension and 41 normal control. NAG values were measured before therapy and after 2 months of antihypertensive therapy. The mean urinary NAG value (±S.E.) for the normo-tensive subjects was 31.6±2.42nmol hr-1mg creatinine-1. The mean value for the untreated hypertensive subjects was 121±9.09 (P<0.01). Systolic blood pressure was directly correlated with NAG levels. The urinary level of NAG in stage II hypertension (146±11, 16 nmol hr-1mg creatinine-1) was higher than that in the normal control (31.6±2.4nmol hr-1mg creatinine-1 , P<0.01), and stage I hypertension (81.8±8.49nmol hr-1mg creatinine-1, P<0.01). Fourteen patients followed for two months attained their ultimate blood pressure reduction within two months (from a mean of 176/110mmHg to one of 138/93mmHg), and therefore the urinary NAG level had changed significantly at two months (from 115,7±15.8 to 75.7±6.55, P<0. 05), These data suggest that NAG is frequently elevated in patients with high blood pressure even though there is no other evidence of renal damage, and that it can be reduced by successful antihypertensive therapy.

인공와우 이식자에서 자극 잡파를 이용한 고장 평가

허승덕,김상렬,안중기,정동근,강명구,Heo, Seung-Deok,Kim, Sang-Ryeol,Ahn, Joong-Ki,Jung, Dong-Keun,Kang, Myung-Koo 한국음성학회 2007 음성과학 Vol.14 No.2

The aim of this study is to analyze the correlation between current intensity and amplitude of stimulus artifact on the cochlear implantee, and to find out basic information to check the device failure. Subjects were a prelingual child and 3 postlingual adults with more than severe hearing losses. The charge-balanced biphasic pulses were presented at stimulus rates of 11 pulses per second, each pulse width of $25{\mu}s$ with monopolar mode(MP1+2). Current intensities were delivered at 27.5, 33.7, 41.3, 50.5, 61.9, $75.8{\mu}A$. Stimulus artifacts were recorded by evoked potential system. This procedure was performed just before the initial stimulation, and then, the amplitude of stimulus artifacts were compared with each current intensity. The amplitude of stimulus artifacts was increased significantly according to the current intensity (p<0.01). The results suggest that the change of the amplitude of stimulus artifact can be used as a good cue to check the device failure in the cochlear implantee.

이중 쐐기형 초음속 흡입구의 압력회복률에 대한 신뢰성 기반 최적설계

이창혁(Chang-Hyuck Lee),안중기(Joong-Ki Ahn),배효길(Hyo-Gil Bae),권장혁(Jang-Hyuk Kwon) 한국항공우주학회 2010 韓國航空宇宙學會誌 Vol.38 No.11

본 연구에서는 이중 쐐기형 초음속 흡입구의 압력회복률에 대한 신뢰성 최적설계를 수행하였다. 주어진 설계영역에서 다양한 설계변수의 불확실성을 고려하여 흡입구의 압력회복률을 확률적으로 모델링하였으며, 목적함수로는 흡입구 항력을 선정하였다. 신뢰성 최적설계에 앞서 전산해석비용을 줄이기 위해 실험계획법과 크리깅 모델을 이용하여 적절한 설계공간을 탐색하였다. 신뢰성 기법의 정확도 검증을 위해 몬테카를로 시뮬레이션을 수행하였으며 이 결과를 신뢰성 기법 결과가 잘 추종함을 확인하였다. 신뢰성 기반 최적설계를 수행한 결과, 설계변수의 불확실성을 고려함으로써 시스템의 신뢰성을 확보하였다. 시스템 설계의 다양한 불확실성을 고려하기 위해서는 신뢰성 기반 최적설계가 유용한 접근방법임을 확인할 수 있었다. In this study, RBDO(Reliability Based Design Optimization) was performed for a supersonic double-wedge inlet. By considering uncertainty of design with given design space, the pressure recovery was transformed into the probabilistic constraint while the inlet drag was considered as a deterministic objective function. To save computational analysis cost and to search good design space, Latin-Hypercube design of experiment and the Kriging model were incorporated and then RBDO was performed. Monte-Carlo simulation was performed to verify the accuracy of AFORM(Advanced First Order Reliability Method). It was found that AFORM result agreed very well with the Monte-Carlo simulation result. The system reliability was guaranteed by considering uncertainty of the design variables. In case of considering diverse uncertainty of system design, RBDO was found to be useful.

B형 만성활동성간염 환자에서 Leukoryte Adherence Inhibition ( LAI ) 반응과 그와 관련된 Prostaglandin E2 ( PGE2 ) 직 변동

허영상(Yeong Sang Heo),안득수(Deuk Soo Ahn),김대곤(Dae Ghon Kim),안중기(Joong Ki Ahn),송석현(Suk Hyun Song),이남심(Nam Sim Lee) 대한내과학회 1989 대한내과학회지 Vol.36 No.1

N/A A leukocyte adherence inhibition (LAI) assay was performed for the evaluation of cellular immune dysfunction in patients with chronic active hepatitis (CAH) B and the effects of prostaglandin E, (PGE,), endogenous and exogenous immune modulator, on LAI reaction were investigated. 1) In patients with CAH, the non adherence index (LAI) was 18.8±1.7 (mean±SE)%, and there was a significant (p<0.001) decrease of LAI as compared to 48.8±1.6% in the antibody positive healthy control group. In chronic HBV carriers LAI was 6.2±1.5%. There was no significant difference in contrast to 8.8±1.3% in the negative normal control group. 2) Changes of the endogenous PGE2 level in the supernatant of the mononuclear cell mixture for LAI were measured and the differences of PGE, levels by specific antigen stiumlation (HBs) and by non specific antigen stimulation (OVA) were calculated. In CAH the difference was -11.4±3.6pg/ml and this was significantly decreased as compared to 19.3±6.3 pg/ml in the positive control group. In chronic HBV carriers the difference was 6.2±1.5 pg/ml and was as low as that in the negative normal control. 3) When only exogenous PGE2 was added to the mononuclear cell mixture, significantly (p<0.01) elevated NAI was observed as compared to the untreated control. On treatment with exogenous PGE, and indomethacin (PG inhibitor), or exogenous PGE, and LiCl (adenyl cyclase inhibitor), inhibitions of increasing NAI were observed. From the above results, in patients with CAH, LAI reflected cellular immunity was declined and this might be related to decreased release of endogenous PGE2 Furthermore, it was suggested that endogenous and exogenous PGE2 should accentuate LAI reaction.

만성 간염 환자에서 S-Adenosyl-L-Methionine 의 임상효과

안중기,하종영,김대곤,안득수 의과학연구소 1988 全北醫大論文集 Vol.12 No.1

전북 의대 부속병원 내과에서 입원 치료중이거나 외래에서 통원 치료를 받고 있는 간침생검상 만성 활동성 간염환자 8명과 임상적으로 만성 간염으로 추정되는 22명을 대상으로 SAMe와 Vit-C를 각각 30일간 매일 투여하여 다음과 같은 결과를 얻었다. SAMe 투여군 (n=21)에서 AST는 118.3±38.6U/L에서 71.4±25.2U/L로 (P<0.05), ALT는 263.14±69.3U/L에서 13.5± 57.3(P<0.01)로 통계학적으로 유의한 감소를 보였다. 21명중 16명에서 AST와 ALT의 지속적인 감소를 보였으며 5명에서는 투여 경과중 경도의 증가를 보였다. VIt-C 투여군 (n=9)에서는 투여 경과 중 유의하지 않는 증가 또는 감소를 보였다. 이상의 결과는 SAMe 투여로 만성 간염 환자군에서 76%의 치료 효과를 보여서 SAMe의 만성 간염 치료에 유용하게 사용될 것으로 사료된다.

Retinoic Acid 가 Bromodeoyuridine 표지 Hep G2 간암 세포주의 세포 주기 역동성에 미치는 효과

안득수,김대곤,안중기,장동석,이수택,김이엽,이세라 대한내과학회 1993 대한내과학회지 Vol.45 No.5

Objectives: Measuring the total cellular DNA and BrdU content simultaneously by using the monoclonal antibody to BrdU and propidium iodide (PI) has been possible. So it has been advanced that the making a correct diagnosis and the predicting a prognosis in cancer as well as the evaluation of the responses of anti-cancer therapy, We intended to exam new anticancer chemotherapeutic agent, retinoic acid (RA) which has been reported to show antiproliferative effect and also differentiation-inducing effect on tumor cells, for the treatment of the hepatocarcinoma prevailed in our country. This study was designed to probe beneficial effects of RA for preventing tumor recurrence and secondary metastasis through the application of it to recently wide spreaded chemoembolization therapy in heaptomas. Methods: A semi-logarithmic plot of the proliferation was made in Hep G2 cells, and the changes of the proliferative activities from adding RA to the medium were evaluated in according to various concentrations of it. After staining the Hep G2 cells with fluoroscence conjugated anti-BrdU and PI, the tumor cell kinetics was analyzed and the effects of RA on the changes of the kinetics were also evaluated in the univariate and bivariate distribution of flowcytometric measurement of DNA content/BrdU incorporation. Results: Hep G2 cells stained with FITC conjugated anti-BrdU and with propidium iodide (PI), were anal- yzed in the distribution of bivariate BrdU/DNA content or univariate DNA content to investigate the tumor kinetics. Data from this expreiments showed that actual doubling time (Td) is 51 hrs, potential doubling tiem (Tpot) is 29 hrs, the mean DNA synthesis time (Ts) is 9. 3 hrs, the labelling index is 27.6%. From the evaluation of the effects of RA, known to enhance the differentiation and inhibit the growth of tumor cells on Hep G2 cells, RA inhibited the proliferation of Hep G2 cells significantly and the inhibition lasted for 10 days. To understand the growth inhibition of RA on Hep G2 cells in terms of tumor cell kinetics, bivariate BrdU/DNA content distributions were analyzed. Early stage of culture (day 1) showed increase in S phase percentage of cells and decrease in Go/G1 phase. But late stage of culture (day 4) show decrease in S phase percentage of cell to minimum and increase in Go/G1 phase at either 1 μM or 0.1 μM concentration of RA as compared with those of control. The influences on cell cycle and the antiproliferative effect were more pronounced at 1 μM than at 0.1 μM concentration. Conclusion: These results demonstrated that RA inhibits the proliferation of Hep G2 cells and the anti-proliferative effect is suggested to be driven from arresting cell cycle progression from Go/G1 to S-phase in tumor cell kinetics.

14 Gauge 절단침과 22 Gauge 흡인침을 사용한 경피적 폐침생검술의 비교

김원호,조광호,이양근,유관희,안중기,송석현 대한내과학회 1987 대한내과학회지 Vol.32 No.3

Percutaneous needle lung biopsy was done in pulmonary or mediastinal lesions using 22Gauge fine aspiration needle or ld Gauge cutting needle. 1) The results were as follows, men were 27, women were 17, mean age was 56±12 years. 2) Mediastinal lesions were in 6 patients and pulmonary lesions were in 3B patients. Among pulmonary lesions, right lungs were involved in 25 patients and left lungs were involved in the rest of 13 patients. 3) In 14 patients, the lesions were less than 4 cm in diameter, and in 30 patients, the lesions were larger than 4~cm, but the size of the lesions was not correlated with malignancy. 4) Malignancy was noted in 17 patients(58%) among 31 patients with respiratory symptoms and in 11 patients (58%) among 19 patients with respiratory symptoms with in 3 months. 5) Overall success rate was 84%, 93.3% with cutting needle and 64.3/ with aspiration needle. 6) Benign lesions were 18(48,6%), maliant lesions were 19(51.4%). Cell type diagnosis was possible in 90% with cutting needle and 30% with aspiration needle. 7) Complications were minimal, overall complication rate 9%, mild pneumothorax occured in one patient(2%) and mild hemoptysis occured in 3 patients(7%). With cutting needle, complication rate was 10%, mild pneumothorax in one patient and mild hemoptysis in 2 patients With aspiration needle, complition rate was 7%, mild hemoptysis in one patient. 8) Percutaneous needle lung aspiration biopsy was difficult for the diagnosis of benign because of only a scant specimen could be obtained and the diagnosis of malignant lesions depended on pap. smear. Percutaneous needle lung cutting biopsy under chest C-T and biplane fluroscopy had lower complication rate and high diagnostic succsess rate also useful for the diagnosis of benign lesion.