카드륨 독성에 대한 부추 (Allium Odorum L.) 의 방어효과

안령미(Ryoung Me Ahn),김완태(Wan Tai Kim),이희성(Hi Sung Lee) 한국환경보건학회 1991 한국환경보건학회지 Vol.17 No.2

This study aimed to find out the effect of freeze -drying leek against cadmiurn poisoning on the cholesterol and enzyme activities in serum and superoxide radical, SOD and catalase in liver and kidney of the rnale rats during the administrered period. ln this experiment, male rats of SPragμe-Dawley strain were used. The rats were divided into 3 groups which were fed different1y either for 5 weeks or for 10 weeks : basal diet, basal diet and cadmium in water and 3 % leek added diet and cadmium in water. Cadmium was administered ad libltum 100 ppm CdCh in water. The followings are the results of this experiment. l. Leek reduced the cholesterol and the activities of GPT increase resulted from cadmium treatment. 2. Leek reduced the rate of cadmium in liver and kidney. 3. Leek reduced the activities of SOR and catalase in liver and kidney, while it enhanced the activities of SOD. 4. Leek reduced the necrosis and swelling in liver and kidney casused by cadmium treatment. This experiment showed that leek -addition group had protective effect agair:st cadmium pois oning and increased ALPase activities in serum. Leek alleviated GPT activities m serum and cad•rnium concentration, necrosis, and swelling in liver and kidney, Therefore, this experiment con c1uded that leek has defenslve power against cadmium poisoning.

보문 : 자외선에 의한 비타민 D3 합성과 직물 ( 제 1 보 )

안령미(Ryoung Me Ahn),송명견(Myung Kyun Song) 한국의류학회 1997 한국의류학회지 Vol.21 No.5

한국 성인의 요중 크레아티닌 농도 변화에 대한 성, 연령 그리고 체질량지수(BMI)의 관련성 연구

이진헌,안령미,Lee, Jin-Heon,Ahn, Ryoung-Me 한국환경보건학회 2010 한국환경보건학회지 Vol.36 No.3

The purpose of this study was to investigate the relevance of gender, age, and BMI (Body Mass Index) to changes in the urinary creatinine concentration in Korean adults. We recruited and surveyed 2,156 persons $\geq$20 years of age from 98 districts across the country, and collected urine samples for analyzing the creatinine concentration. Participants were 41.6% men and 58.4% women. In terms of age, the percentage of the population in their 20's and $\geq$60 years of age was relatively similar, with 13.6% and 17.8%, respectively, while the percentages in their 30's, 40's and 50's were also relatively similar, with 22.0%, 24.0%, and 22.6%, respectively. The proportion of participants in their 20's and 30's who were underweight (according to the BMI) was 25% and 19.2%, respectively, but only 6.2~6.4% of those $\geq$40 years of age were underweight. This showed that the underweight proportion in elder generations was less than that in younger generations. Meanwhile, the proportion who were overweight was 13.7%, 18.8%, 28.6%, 27.9%, 32.0% when the participants were in their 20's, 30's, 40's, 50's and $\geq$60 years of age, respectively (p<0.000). This showed that the overweight proportion increased along with increasing age. The overall urinary creatinine concentration was 10 mg/dl (arithmetic mean: AM), and 92.2 mg/dl (geometric mean: GM). The urinary creatinine concentration in men (132.6 mg/dl, AM) was significantly higher than that in women (93.3 mg/dl, AM (p<0.000)). Showing a similar trend in men and women, urinary creatinine concentrations were highest when the participants were in their 20's (135.6 mg/dl, AM), and tended to decrease with increasing age. Urinary creatinine concentrations in overweight and obese subjects (AM of 117.9 mg/dl and 118.0 mg/dl, respectively) were significantly higher than in other groups, and this trend was similar in men and women. In conclusion, we found that urinary concentrations were significantly affected by gender, age, and BMI, and that care should therefore be exercised when correcting urinary metabolites according to the urinary creatinine concentration.

Antiretroviral Effects of 2',3'-Dideoxycytidine and Recombinant $Interferon-{\alpha}-A$ on the Infection of Anemia-inducing Murine Friend Virus

안형수,안령미,김동섭,Ann, Hyung-Soo,Ahn, Ryoung-Me,Kim, Dong-Seop The Korean Society of Pharmacology 1995 대한약리학잡지 Vol.31 No.3

Anemia-inducing strain of Friend virus (FVA)는 적혈구 progenitor cell의 증식을 촉진하는 생쥐 retrovirus의 일종이다. FVA에 감염된 생쥐는 생성이 촉진된 progenitor cell이 분화되지 못하고 비장내에 축적되므로서 비장비대를 초래한다. 이에 본 실험에서는 FVA에 감염된 생쥐의 비장비대를 지표로 사용하여 2',3'-dideoxycytidine (ddC) 및 $interferon-{\alpha}-A\;(rIFN-{\alpha}-A)$의 항retrovirus효과를 측정하였다. 매일 ddC (100 mg/kg) 및 $rIFN-{\alpha}-A$ (10 KU/mouse)를 각각 단독 또는 병용하여 18일간 복강내 투여시 비장의 비대가 각각 15.1%, 52.7%, 61.6% 억제되었다. 또 다른 실험군으로 ddC를 식수중에 용해하여 (0.1 mg/ml) 경구로 18일간 투여시, 그리고 ddC의 경구투여와 병용하여 $rIFN-{\alpha}-A$을 위와 마찬가지 용량으로 복강내 투여시, 비장비대를 각각 38.4% 및 83.2% 억제하였다. 이 결과는 ddC의 투여시 복강내 주사보다는 경구투여가 더 유효하며, ddC와 $rIFN-{\alpha}-A$는 병용투여시 상가적인 효과가 있음을 제시한다. ddC 투여시 progenitor cell의 특성상 변화를 검토하기 위해, $Ca^{++}$ uptake $[^3H]cyclohexyladenosine$ (CHA) binding 실험을 실시하였다. CHA bindng 실험결과 성숙된 적혈구에서는 저친화성의 결합부위 하나뿐인데 반해, progenitor cell에서는 고친화성과 저친화성의 두가지 결합부위를 나타내었다. $Ca^{++}$ uptake 측정결과 성숙된 적혈구에 비해 대조군의 정상적인progenitor cell은 약 20배 증가를 나타내었으며, ddC를 연속투여한 군에서도 유사한 결과를나타내었다. 이때 CHA에 의한 $Ca^{++}$ uptake의 억제효과를 측정한 바, ddC 100 mg/kg 투여군의 경우 76%로 대조군의 86% 보다 억제효과가 크게 나타났으며, 이들 모두는 adenosine 길항약인 theophylline의 전처치시 대조군과 유사하게 회복되었다. The anemia-inducing strain of Friend virus (FVA) is a murine retrovirus which stimulates the proliferation of erythroid progenitor cells. The progenitor cells synthesized by FVA-stimulation are unable to proceed with differentiation and accumulate in the spleen resulting in splenomegaly in infected mice. Using FVA-inoculated mice as a model, we have investigated the antiretroviral effects of 2',3'-dideoxycytidine (ddC) and recombinant $interferon-{\alpha}-A\;(rIFN-{\alpha}-A)$ on FVA infection. The extent of the infection was determined by measuring the weights of the spleens. Daily intraperitoneal injection of ddC (100 mg/kg body weight), $rIFN-{\alpha}-A$ (10 KU/mose) and the combination of both drugs to FVA inoculated mice for 18 days resulted in suppression of the growth of spleens by 15.1%, 52.7% and 61.6%, respectively. When ddC was dissolved in drinking water (0.1 mg/ml) and administered to a group of FVA inoculated mice ad libitum, and $rIFN-{\alpha}-A$ (10 KU/mouse) was intraperitoneally injected daily to another group of ddC (0.1 mg/ml) drinking mice for 18days, the growth of spleens was suppressed by 38.4% and 83.2%, respectively. These results indicate that administration of ddC via drinking water is more effective in suppressing FVA infection than the daily injection of ddC, and that the combined effects ddC and $rIFN-{\alpha}-A$ are not synergistic but additive. In order to determine whether ddC treatment alters the characteristic of the progenitor cells with respect to $Ca^{++}$ uptake, $Ca^{++}$ uptake in erythroid cells and the effect of cyclohexyladenosine (CHA) on the $Ca^{++}$ uptake were studied. $Ca^{++}$ uptake in the erythroid progenitor cells was about 20-fold greater than in mouse erythrocytes and the inhibition of $Ca^{++}$ uptake by CHA was the greatest in the progenitor cells from FVA infected mice which were treated with ddC. The inhibition was obviated by theophylline. Results of CHA binding studies showed that the erythroid progenitor cells contain both high and low affinity CHA binding sites, whereas mose erythrocytes contain only the low affinity CHA binding sites.

ICR mouse에 있어 UVB조사로 유도된 접촉 과민반응에 대한 EGb 761의 억제 효과

최욱희,안형수,안령미,Choi, Wook-Hee,Ann, Hyoung-Soo,Ahn, Ryoung-Me 한국환경보건학회 2005 한국환경보건학회지 Vol.31 No.1

Exposure of skin to UVB radiation can cause the induction of inflammation and impairment of contact hypersensitivity(CHS) response. Several studies have shown that polyphenolic compounds isolated from EGb 761 afford protection against UVB. In this study, we demonstrated that topical application of EGb 761, before 1MED(1.4 KJ/$m^2$), 1.5MED (2.1 KJ/$m^2$), 2MED (2.8 KJ/$m^2$) of UVB exposure to ICR mice prevented UVB-induced inflammation and inhibition of the contact hypersensitivity response. The skin-fold swelling from 1MED, 1.5MED, 2MED of UVB exposure highly significantly increased after twice irradiation. Topical application of EGb 761(0.1%, 1%, 4%), 5 days prior to UVB exposure reduced skin thickness compared to non-treated mice. Exposure of shaved abdominal skin of mice to 1MED, 1.5MED and 2MED of UVB radiation resulted in suppression of contact sensitization through the skin to 56.23%, 65.12%, 74.02%, compared to normal unirradiated skin. Topical application of EGb 761(0.1%, 1%, 4%), 5 days prior to or 5 days after exposure to 1MED and 2MED of UVB resulted in protection against suppression of contact hypersensitivity in mouse dorsal skin. These protective effects were dependent on the dose of EGb 761 employed. The present study show that EGb 761 protect UVB-induced inflammation and immune suppression. Also, we suggest that EGb 761 can provide protection from photoimmunosuppression.

ICR mouse의 피부와 간에서 자외선 B파에 의한 Heme Oxygenase-1의 유도

최욱희,김태환,안령미,Choi, Wook-Hee,Kim, Tae-Hwan,Ahn, Ryoung-Me 한국환경보건학회 2008 한국환경보건학회지 Vol.34 No.1

The induction of heme oxygenase-1(HO-1) by UV radiation provides a protective defence against oxidative stress, and has been well demonstrated in skin irradiated with UVA, but not UVB. In this study, we show that the induction of cutaneous HO-l can be attributed to UVB radiation. The expression of HO-1 mRNA was assessed in vivo by reverse transcription-polymerase chain reaction (RT-PCR) analysis, and HO-1 enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 1d to 3d after UVB $(3KJ/m^2)$ exposure. The results of gene expression were same pattern of HO-1 enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 4d after UVB exposure, but HO-1 activity in liver increased to 2d. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissue directly or indirectly from oxidative stress.

Anemia-inducing Murine Friend Virus 감염에 대한 2 ,3 -dideoxycytidine 및 Interferon-α-A의 항retrovirus효과

안형수(Hyung Soo Ann),안령미(Ryoung Me Ahn),김동섭(Dong Seop Kim) 대한약리학회 1995 대한약리학잡지 Vol.31 No.3

Anemia-inducing strain of Friend virus (FVA)는 적혈구 progenitor cell의 증식을 촉진하는 생쥐 retrovirus의 일종이다. FVA에 감염된 생쥐는 생성이 촉진된 progenitor cell이 분화되지 못하고 비장내에 축적되므로서 비장비대를 초래한다. 이에 본 실험에서는 FVA에 감염된 생쥐의 비장비대를 지표로 사용하여 2 ,3 -dideoxycytidine (ddC) 및 interferon-α-A (rIFN-α-A)의 항retrovirus효과를 측정하였다. 매일 ddC (100 mg/kg) 및 rIFN-α-A (10 KU/mouse)를 각각 단독 또는 병용하여 18일간 복강내 투여시 비장의 비대가 각각 15.1%, 52.7%, 61.6% 억제되었다. 또 다른 실험군으로 ddC를 식수중에 용해하여 (0.1 mg/ml) 경구로 18일간 투여시, 그리고 ddC의 경구투여와 병용하여 rIFN-α-A을 위와 마찬가지 용량으로 복강내 투여시, 비장비대를 각각 38.4% 및 83.2% 억제하였다. 이 결과는 ddC의 투여시 복강내 주사보다는 경구투여가 더 유효하며, ddC와 rIFN-α-A는 병용투여시 상가적인 효과가 있음을 제시한다. ddC 투여시 progenitor cell의 특성상 변화를 검토하기 위해, Ca⁺⁺ uptake [³H]cyclohexyladenosine (CHA) binding 실험을 실시하였다. CHA bindng 실험결과 성숙된 적혈구에서는 저친화성의 결합부위 하나뿐인데 반해, progenitor cell에서는 고친화성과 저친화성의 두가지 결합부위를 나타내었다. Ca⁺⁺ uptake 측정결과 성숙된 적혈구에 비해 대조군의 정상적인progenitor cell은 약 20배 증가를 나타내었으며, ddC를 연속투여한 군에서도 유사한 결과를나타내었다. 이때 CHA에 의한 Ca⁺⁺ uptake의 억제효과를 측정한 바, ddC 100 mg/kg 투여군의 경우 76%로 대조군의 86% 보다 억제효과가 크게 나타났으며, 이들 모두는 adenosine 길항약인 theophylline의 전처치시 대조군과 유사하게 회복되었다. The anemia-inducing strain of Friend virus (FVA) is a murine retrovirus which stimulates the proliferation of erythroid progenitor cells. The progenitor cells synthesized by FVA-stimulation are unable to proceed with differentiation and accumulate in the spleen resulting in splenomegaly in infected mice. Using FVA-inoculated mice as a model, we have investigated the antiretroviral effects of 2 ,3 -dideoxycytidine (ddC) and recombinant interferon-α-A (rIFN-α-A) on FVA infection. The extent of the infection was determined by measuring the weights of the spleens. Daily intraperitoneal injection of ddC (100 mg/kg body weight), rIFN-α-A (10 KU/mose) and the combination of both drugs to FVA inoculated mice for 18 days resulted in suppression of the growth of spleens by 15.1%, 52.7% and 61.6%, respectively. When ddC was dissolved in drinking water (0.1 mg/ml) and administered to a group of FVA inoculated mice ad libitum, and rIFN-α-A (10 KU/mouse) was intraperitoneally injected daily to another group of ddC (0.1 mg/ml) drinking mice for 18days, the growth of spleens was suppressed by 38.4% and 83.2%, respectively. These results indicate that administration of ddC via drinking water is more effective in suppressing FVA infection than the daily injection of ddC, and that the combined effects ddC and rIFN-α-A are not synergistic but additive. In order to determine whether ddC treatment alters the characteristic of the progenitor cells with respect to Ca⁺⁺ uptake, Ca⁺⁺ uptake in erythroid cells and the effect of cyclohexyladenosine (CHA) on the Ca⁺⁺ uptake were studied. Ca⁺⁺ uptake in the erythroid progenitor cells was about 20-fold greater than in mouse erythrocytes and the inhibition of Ca⁺⁺ uptake by CHA was the greatest in the progenitor cells from FVA infected mice which were treated with ddC. The inhibition was obviated by theophylline. Results of CHA binding studies showed that the erythroid progenitor cells contain both high and low affinity CHA binding sites, whereas mose erythrocytes contain only the low affinity CHA binding sites.

천연물 도포가 UVB 파로 손상된 C57BL/6 mouse 피부의 색소침착과 염증생성에 미치는 영향

최욱희,안형수,최태윤,진소영,안령미,Choi, Wook-Hee,Ann, Hyoung-Soo,Choi, Tae-Youn,Jin, So-Young,Ahn, Ryoung-Me 한국환경보건학회 2006 한국환경보건학회지 Vol.32 No.5

Ultraviolet(UV) radiation causes a variety of biological effects on the skin, including inflammation, pigmentation, photoaging and cancer. Free radicals are involved in inflammatory skin reactions induced by UVB radiation. In this study, we investigated the effects of antioxidants(Tea, Korean red ginseng, Ginkgo biloba extract) on UVB-induced skin damage. Tea, KRG and EGb 761 were topically treated to dorsal skin of ICR mouse. The mice were also treated soon after IMED ($1.4KJ/m^{2}$) of UVB irradiation. Skin pigmentation of irradiated mouse was observed by a chromameter after 2 weeks. Topical application of Tea, KRG and EGb 761 for 2 weeks decreased skin pigmentation compared to DVB control group(p<.05). Tea, KRG and EGb 761 also reduced UVB-induced infiltration of inflammatory cells. These results showed that Tea, KRG and EGb 761 as a topical application may have preventive effect against UVB-induced skin damage.

여름용 모자의 UVA 투과량

송명견(Song Myung Kyun),한문정(Han Mun Jung),안령미(Ahn Ryoung Me) 한국지역사회생활과학회 2003 한국지역사회생활과학회지 Vol.14 No.3

Monocrotaline에 의해 유발된 폐고혈압 흰쥐에 있어 Enalapril 및 Ginkgo biloba Extract (EGb 761)의 병용 투여시 억제효과

이영미(Young Mi Lee),안형수(Hyung Soo Ann),임세진(Se Jin Lim),안령미(Ryoung Me Ahn) 대한약학회 1999 약학회지 Vol.43 No.4

Effects of Ginkgo biloba extract(EGb 761) on the anti-pulmonary hypertensive action of enalapril were evaluated in rats. Pulmonary hypertension was induced by monocrotaline treatment (60mg/kg, I.p.) in normotensive rats. In the systolic pulmonary artery pressure, the control group was 33 +/- 2mmHg, comparing to the normal group of 19 +/- 1 mmHg. That of enalapril group(20mg/kg/day, p.o.) was 26 +/- 2 mmHg, and that of combined group of enalapril and Ginkgo biloba extract (EGb 761;60mg/kg/day, p.o.) was 20 +/- 2 mmHg. In the isolated lung preparation, acetylcholine, which was endothelium dependent vasodilator, induced the decrease of pulmonary artery perfusion pressure(-2.0 +/- 0.7mmHg) in normal group, but the increase of that of 3.4 +/- 0.6 and 3.0 +/- 0.9mmHg in control and enalapril group, respectively. And that of the combined group was -0.5 +/- 0.2mmHg. In the isolated pulmonary artery, acetylcholine(10-5M) induced the relaxation of 65 +/- 6% in normal group, but 15 and 8% in control and enalapril group, respectively. And that of the combined group was resulted 55 +/- 2%. These results suggested that co-administration of Ginkgo biloba extract(EGb 761) potentiated the anti-pulmonary hypertensive effects of enalapril through the increase of pulmonary vasodilation due to the protection of endothelial cell by antioxidant action of Ginkgo biloba extract (EGb 761).