자외선 B파가 조사된 Hairless Mouse에서 탄닌의 피부 독성 억제효과 및 Heat shock protein 70의 생성억제 효과

안령미 강원대학교 종합약학연구소 1998 약학연구 Vol.10 No.-

카드뮴에 중독된 웅성 흰 쥐의 간, 신장 및 고환의 Glutathione Peroxidase, Glutathione Reduetase, and Glutathione-s-Transferasea의 활성도와 부추의 효과

안령미 한국환경보건학회 1992 한국환경보건학회지 Vol.18 No.1

Effect of freeze drying leek against cadmium poisoning on glutathione peroxidase, on glutathione reductase and on glutathione-s-transferase in liver, kidney and testes of the male rats during the administered period. In this experiment, male rats of Sprague-Dawley strain were used. The rats which were fed for 15 weeks were divided into 4 groups basal diet 3% leek added diet basal diet and cadmium in water and 3% leek added diet and cadmium in water. Cadmium was administered ad libitum 100ppm CdCl$_{2}$ in distilled water. The followings are the result of this experiment. 1. Leek enhanced the glutathione peroxidase activities which were reduced by cadmium treatment in liver, kidney and testes but not significance. 2. Leek reduced glutathione reductase activities which were incresed by cadmium in liver, kidney and testes. 3. Leek incresed the activities of glutathfone-s-transferase in liver but not in kidney and but not in testes. 4. Leek incresed glutathione concentration which was decresed by cadmium treatment in liver and kidney but not testes. This experiment showed that leek-addition group had protective effect against cadmium poisoning and alleviated GR and glutathione-s-transferase activities in tissues. Leek incresed activities of glutathione peroxidase in liver, kidney and testes but not significance. Therefore, this experiment concluded that leek defensive power against long term cadmium poisoning.

카드뮴 독성에 대한 부추(Allium Odorum L.)의 방어효과

안령미,김완태,이희성 한국환경보건학회 1991 한국환경보건학회지 Vol.17 No.2

This study aimed to find out the effect of freeze-drying leek against cadmium poisoning on the cholesterol and enzyme activities in serum and superoxide radical, SOD and catalase in liver and kidney of the male rats during the administrered period. In this experiment, male rats of Sprague-Dawley strain were used. The rats were divided into 3 groups which were fed differently either for 5 weeks or for 10 weeks:basal diet, basal diet and cadmium in water and 3 % leek added diet and cadmium in water. Cadmium was administered ad libiturn 100 ppm CdCl$_{2}$ in water. The followings are the results of this experiment. 1. Leek reduced the cholesterol and the activities of GPT increase resulted from cadmium treatment. 2. Leek reduced the rate of cadmium in liver and kidney. 3. Leek reduced the activities of SOR and catalase in liver and kidney, while it enhanced the activities of SOD. 4. Leek reduced the necrosis and swelling in liver and kidney casused by cadmium treatment. This experiment showed that leek-addition group had protective effect against cadmium poisoning and increased ALPase activities in serum. Leek alleviated GPT activities in serum and cadmium concentration, necrosis, and swelling in liver and kidney. Therefore, this experiment concluded that leek has defensive power against cadmium poisoning.

직물의 자외선차단과 세포에 미치는 방호효과

안령미,이수진,송명견 한국의류학회 1997 한국의류학회지 Vol.21 No.4

The purpose of the study was to investigate a transmittance rate of UVB (Ultraviolet B) through summer fabrics and a protection rate of summer fabric from UVB. The subjects were randomly selected 159 fabrics from Korean common summer fabrics. The protection rates of 159 fabrics from UVB were measured by UVB lamp and UVB sensor, and 14 fabrics among these fabrics were selected for an assay of MTT(3-[4, 5-dimethylthiazol-2-yl] -2, 5 -diphenyltetrazolium). The protection rate of fabrics from cell toxicity of UVB was measured by investigating the difference of the amount of cell toxic substance on between fabrics covered with and without HeLa cell. The average protection rate of 159 fabrics from UVB was 95.08%. As result findings, three negative correlations were found between: 1) the transmittance rate of UVB and the amount of MTT on fabrics (y=0.0373+0.00518 x, r=-0.9323, p<0.001); 2) the air permeability of fabrics and the amount of MTT (r= -0.79, p<0.01); 3) the air permeability of fabrics and the protection rate of fabrics from UVB (r=0.89, p<0.01). However, there was no effect of thickness of fabrics on the protection rate from UVB and the amount of MTT.

자외선에 의한 비타민 D_3 합성과 직물 (제2보) : 동물 실험을 통하여 The animal study

안령미,김정현,송명건 한국의류학회 1998 한국의류학회지 Vol.22 No.5

Because there is a great. concern today about the damaging effect of chronic exposure to sunlight the use of sunscreen providing the photoprotection effect against ultraviolet (UV) was widely increased. As a result of common use of level of photosynthetic Vit. D_3 in human skin decreased these days. In our experiment the animals covered with fabrics with 50% (fabric B) and 100% (fabric A) protection rate against ultraviolet B (UVB) were used to measure serum 25(OH)D_3, ALP, total clacium and phosphorus. Vitamin D deficiency diet group had no effect on concentration of serum phosphorus. But the concentrations of serum 25(OH)D_3 and total calcium were more decreased in vitamin D deficiency diet rats than in normal diet rats. Alkaline phosphatase activity in sunlight irradiated groups covered with 50% (fabric B) and 100% (fabric A) UVB protection fabrics was more significantly decreased than vitamin D deficiency diet group. In conclusion, sunlight irradiateted groups were compared to effective to protect born disease due to the Vit. D deficeincy group.

비타민 D 결핍식이 쥐에서의 태양광성 조사에 의한 malondialdehyde 생성측정과 alkaline phosphatase 변화에 관한 연구

안령미,김정현,장동덕 대한보건협회 1998 대한보건연구 Vol.24 No.1

비타민 D는 칼슘과 인의 세포외액농도를 정상범위로 유지하는 비타민이다. 또한 골의 파괴와 형성과도 관계가 있다. 비타민 D는 두 가지 공급원에 의해서 공급되어 지는데, 첫 번째는 식이에 의한 섭취, 두 번째는 피부에서의 합성이다. 본 연구에서는 일반식이와 비타민 D 결핍식이 쥐에 태양광선을 조사하였을 경우 혈청 25(OH)D_3, 칼슘, 인, alkaline phosphatase(ALP)의 변화를 연구하고자 하였다. 또한 실험동물에 태양광선의 조사가 과산화지질의 지표로 사용되는 malondialdehyde 농도에 미치는 영향을 관찰하였다. 어린 암컷의 SD rats를 1)일반식이, 2) 일반식이+태양광선, 3) 비타민 D 결핍식이, 4) 비타민 D 결핍식이+태양광선의 4군으로 분류하였다. 비타민 D 결핍식이는 혈청 칼슘과 인의 농도에 영향을 미치지 않았다. 하지만 혈청 25(OH)D_3 농도의 경우 일반식이군에 비하여 비타민 D 결핍식이 군에서 유의하게 감소하였으며, 비타민 D 결핍식이군에 태양광선을 조사하였을 때 증가하였다. ALP 활성의 경우 일반식이나 비타민 D 결핍식이군에 비해 태양광선을 조사한 군들에서 감소를 보였다. 또한 일반식이보다 비타민 D 결핍식이군에서 ALP의 활성이 낮았다. Malondialdehyde의 생성은 태양광선 비조사군에 비하여 조사군에서 감소하였다. 결론적으로 실험동물에 태양광선을 조사한 경우 칼슘, 인, 25(OH)D_3의 혈청농도에는 변화를 주지 않았지만 골질환의 인지지표인 ALP에 있어서는 유의한 차이를 나타냈다. 즉 비타민 D 결핍식이를 공급한 쥐에 있어 태양광선을 조사하였을 경우에는 통계적으로 유의하게 ALP가 감소하였다. 이는 비타민 D의 결핍으로 인한 ALP의 상승을 태양광선의 조사에 의해 완화된다는 것을 나타낸다. Vitamin D is a unique vitamin and hormone which maintains extracellular fluid concentrations of calcium and phosphorus within the normal range. Additionally, it is related with bone destruction and bone formation. The vitamin D is supplied with two sources 1) ingested in the diet, 2) produced in the skin. We now have examined the effect of sunlight exposure on serum 25(OH)D_3, calcium, phosphorus and alkaline phosphatase in rat with normal or vitamin D deficiency diet. Also we have observed the malondialdehyde concentration as an index of lipid peroxidation when rats was irradiated with sunlight. Weanling female Sprague Dawely rats were randomly divided into 4 groups: 1) normal diet, 2) normal diet with sunlight exposure, 3) vitamin D deficiency diet, 4) vitamin D deficiency diet with sunlight exposure. Vitamin D deficiency diet had no effect on concentration of serum calcium and phosphorus. But the concentrations of serum 25(OH)D_3 was decreased in rat with vitamin D deficiency diet compared to rats with normal diet. Serum 25(OH)D_3 concentration was increased by sunlight exposure in vitamin D deficiency diet group. In alkaline phosphatase activity, it was decreased in sunlight irradiated groups compared to normal and vitamin D deficiency diet groups. And the activity of alkaline phosphatase was lowered in vitamin D deficiency diet group than normal diet one. Sunlight decreased the synthesis of malondialdehyde in irradiated group compared to non-irradiated group. These results suggest that the sunlight irradiation had lowered the ALP concentration in serum. And it implied that the sunlight exposure may produce an beneficial effect on the bone.

비타민 D 결핍식이 쥐에서의 태양광선 조사에 의한 malondialdehyde 생성측정과 alkaline phosphatase 변화에 관한 연구

안령미,김정현,장동덕 대한보건협회 1998 대한보건연구 Vol.24 No.1

비타민 D는 칼슘과 인의 세포외액농도를 정상범위로 유지하는 비타민이다. 또한 골의 피괴와 형성과도 관계가 있다. 비타민 D는 두 가지 공급원에 의해서 공급되어 지는데, 첫 번째는 식이에 의한 섭취, 두 번째는 피부에서의 합성이다. 본 연구에서는 일반식이와 비타민 D 결핍식이 쥐에 태양광선을 조사하였을 경우 혈청 25(OH)D_3, 칼슘, 인, alkaline phosphatase(ALP)의 변화를 연구하고자 하였다. 또한 실험동물에 태양광선의 조사가 과산화지질의 지표로 사용되는 malondialdehyde 농도에 미치는 영향을 관찰하였다. 어린 암컷의 SD rats를 1)일반식이, 2) 일반식이+태양광선, 3) 비타민 D 결핍식이, 4) 비타민 D 결핍식이+태양광선의 4군으로 분류하였다. 비타민 D결핍식이는 혈청 칼슘과 인의 농도에 영향을 미치지 않았다. 하지만 혈청 25(OH)D_3 농도의 경우 일반식이군에 비하여 비타민 D 결핍식이 군에서 유의하게 감소하였으며, 비타민 D 결핍식이군에 태양광선을 조사하였을 때 증가하였다. ALP 활성의 경우 일반식이나 비타민 D 결핍식이군에 비해 태양광선을 조사한 군들에서 감소를 보였다. 또한 일반식이보다 비타민 D 결핍식이군에서 ALP의 활성이 낮았다. Malondialdehyde의 생성은 탸양광선 비조사군에 비하여 조사군에서 감소하였다. 결론적으로 실험동물에 태양광선을 조사한 경우 칼슘, 인, 25(OH)D_3 의 혈청농도에는 변화를 주지 않았지만 골질환의 인지지표인 ALP에 있어서는 유의한 차이를 나타냈다. 즉 비타민 D 결핍식이를 공급한 쥐에 있어 태양광선을·조사하였을 경우에는 통계적으로 유의하게 ALP가 감소하였다. 이는 비타민 D의 결핍으로 인한 ALP의 상승을 태양광선의 조사에 의해 완화된다는 것을 나타낸다. Vitamin D is a unique vitamin and hormone which maintains extracellular fluid concentrations of calcium and phosphorus within the normal range. Additionally, it is related with bone destruction and bone formation. The vitamin D is supplied with two sources 1) ingested in the diet, 2) produced in the skin. We now have examined the effect of sunlight exposure on serum 25(OH)D_3, calcium, phosphorus and alkaline phosphatase in rat with normal or vitamin D deficiency diet. Also we have observed the malondialdehyde concentration as an index of lipid peroxidation when rats was irradiated with sunlight. Weanling female Sprague Dawely rats were randomly divided into 4 groups : 1) normal diet, 2) normal diet with sunlight exposure, 3) vitamin D deficiency diet, 4) vitamin D deficiency diet with sunlight exposure. Vitamin D deficiency diet had no effect on concentration of serum calcium and phosphorus. But the concentrations of serum 25(OH)D_3 was decreased in rat with vitamin D deficiency diet compared to rats with normal diet. Serum 25(OH)D_3 concentration was increased by sunlight exposure in vitamin D deficiency diet group. In alkaline phosphatase activity, it was decreased in sunlight irradiated groups compared to normal and vitamin D deficiency diet groups. And the activity of alkaline phosphatase was lowered in vitamin D deficiency diet group than normal diet one. Sunlight decreased the synthesis of malondialdehyde in irradiated group compared to non-irradiated group. These results suggest that the sunlight irradiation had lowered the ALP concentration in serum. And it implied that the sunlight exposure may produce an beneficial effect on the bone.

제 3 회 신약개발 연구발표회 초록집 ( 1994 . 4 . 15 ~ 16 ) : 포스터발표 제 2 분과 ( 생명공학 , 약효검색 , 약리대사 ) ; 자외선 B 를 조사한 hairles mouse 피부 단백질의 2 차 전기영동과 유도된 단백질

안령미 한국응용약물학회 1994 학술대회 Vol.1994 No.1

카프뮴 중독자 검색을 위한 메탈로치오네인 분석

안령미 한국환경보건학회 2002 한국환경보건학회 학술대회 자료집 Vol.2002 No.-

카드뮴중독자 검색을 위한 메탈로치오네인 분석 -동물실험을 중심으로-

안령미 한국환경보건학회 2001 한국환경보건학회지 Vol.27 No.4

The purpose of this study was to investigate the metallothionein of acute cadmium poisoning mice as a Cadmium index. Forty male ICR mice were injected with cadmium chloride solution from 1/8LD50 to 1/2LD50 dose. At 24 hours after exposed Cd, I examined Cd and metallothionein (MT) intissues(liver and kidney) and fluids(whole blood and urine) and also measured low molecular proteins, N-acetyl-D-glucosaminidase(NAG) and 2- microglobuline (2-MG) in urine. The concentration of Cd and MT of liver kidney whole blood and urine were increased with dose dependent manner. Urinary Cd and urinary MT and very good significance (p<0.01) and urinary MT had good significance with kidney Cd and NAG but not 2-MG Conclusionally MT in urine was very correlated with kidney Cd and urine Cd, So MT maybe useful as a Cd poisoning index