단보 : 울릉도에서 분리한 미기록 진균 종 보고

안금란 ( Geum Ran Ahn ),김성환 ( Seong Hwan Kim ) 한국균학회 2015 Mycobiology Vol.43 No.1

울릉도는 동해에 위치한 화산섬으로 해양성기후이며 생태적으로 다양한 식물들이 존재하는 곳이다. 이 지역에서 진균에 대한 보고가 미진하여 나리분지의 식물과 도동의 흙 시료로부터 진균을 분리하고 형태학적 방법과 분자생물학적 방법으로 동정하였다. 그 결과 C. aleuritis, H. valdu-nensis, P. uvicola 와 P. caudate가 분리 동정되었다. 이들 진균은 국내에서 처음 보고되는 것이다. Ulleungdo is a volcanic island inhabited by a variety of animals and plants in an oceanic climate in the East Sea. The presence of filamentous fungi has been rarely reported in Ulleungdo. Thus, in this study some plants in the Nari basin and soils in the Dodong region were sampled. Fungi were isolated from the soil and identified by microscopical and molecular biological methods. Coniothyrium aleuritis, Hypocrea valdunensis, Pestalotiopsis uvicola and P. caudate were identified and found to be undescribed species in Korea.

국내에서 분리된 미기록 진균 18종 보고

안금란 ( Geum Ran Ahn ),최민아 ( Min Ah Choi ),김지은 ( Ji Eun Kim ),서은지 ( Eun Ji Seo ),김준영 ( Jun Young Kim ),김성환 ( Seong Hwan Kim ) 한국균학회 2017 韓國菌學會誌 Vol.45 No.4

2016년 버섯 재배사 내 실내공기, 식물병반(동백나무의 잎, 줄딸기, 은방울꽃, 생강나무), 버섯 배양배지 재료, 오염된 가구 등에서 진균을 분리· 동정하였다. 동정된 진균 중 18종이 국내 미기록임을 확인하였다. 동정된 진균은 버섯 재배사 내 실내공기에서 6종, 식물병반에서 6종, 버섯 배양배지 재료에서 5종, 오염된 가구에서 1종이 각각 분리되었다 이들 동정된 균주에 대한 형태학적 특성과 ITS rDNA, 18S rDNA, 28S rDNA, calmodulin gene, translation elongation factor gene, β-tubulin gene 등의 염기서열에 기반한 분자계통학적 관계에 대하여 기술하였다. During a survey of culturable fungi in Korea, 18 unrecorded fungal species were isolated and identified from the indoor air of mushroom cultivation houses, the materials used for preparation of mushroom cultivation media, wild plants, and funitures. This study reports the descriptions of the 18 unrecorded fungal species: Aspergillus creber, Ceratocystis paradoxa, Colletotrichum spaethianum, Coniochaeta velutina, Coprinellus xanthothrix, Epicoccum sorghinum, Leptosphaeria rubefaciens, Myrothecium gramineum, Paraconiothyrium fuckelii, Penicillium erubescens, Penicillium melinii, Penicillium pulvillorum, Penicillium sabulosum, Penicillium turbatum, Pestalotiopsis portugalica, Pilidiella castaneicola, Rachicladosporium pini, and Umbelopsis nana. For all the identified species, the morphological characteristics including the features of colony formed on media, images of light microscopy, and molecular phylogenetic relationships based on nucleotide sequences of the internal transcribed spacer ribosomal DNA (ITS rDNA), 18S rDNA, 28S rDNA, β-tubulin gene, calmodulin gene, and translation elongation factor gene were described.

보문 : 표고버섯 재배사에서 분리한 국내 미기록 진균 보고

안금란 ( Geum Ran Ahn ),권혁우 ( Hyuk Woo Kwon ),고한규 ( Han Kyu Ko ),김성환 ( Seong Hwan Kim ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.1

진균 오염은 톱밥배지를 이용한 실내 표고 재배에 있어서 피해를 주는 요인 중 하나이다. 표고 재배사를 대상으로 진균을 모니터링하는 중, 재배사의 실내공기와 재배사내에서 채집한 버섯파리로부터 여러 진균을 분리 동정하였다. 동정된 진균 중에는 Mucor nidicola, Perenniporia medullapanis, Discosia artocreas, Pseudozyma prolifica, Ascochyta hordei 등을 국내 미기록종으로 보고하고, Penicillium charlesii와 Penicillium brevicompactum의 국내존재를 확인하였다. 본 논문에서는 이들 미기록 균류에 대한 형태적 특성과 더불어 internal transcribed spacer (ITS) rDNA region 또는 calmodulin 유전자 염기서열에 기반한 계통학적 관계에 대해 기술하였다. Fungal contamination is a detrimental factor affecting sawdust media-based shiitake cultivation in greenhouses. During fungal monitoring of greenhouses used for shiitake cultivation, eight fungal species were isolated and identified from indoor air and mushroom flies collected in the greenhouses. The current study reported five species as new in Korea, viz. Ascochyta hordei, Discosia artocreas, Mucor nidicola, Perenniporia medulla-panis, and Pseudozyma prolifica, and confirmed two species, Penicillium charlesii and Penicillium brevicompactum, which were previously recorded in Korea without molecular taxonomic validation. The morphological characteristics and phylogenetic relationships based on nucleotide sequences of the internal transcribed spacer rDNA region or calmodulin gene were described for all identified species.

국내 미기록 진균 5종 보고

안금란 ( Geum Ran Ahn ),김보영 ( Bo Young Kim ),이근식 ( Geun Sick Lee ),현민우 ( Min Woo Hyun ),이찬중 ( Chan Jung Lee ),김성환 ( Seong Hwan Kim ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.4

During a survey of the activities of fungi in steamed sweet potato, stored garlic, agricultural by-products for mushroom cultivation media, and pinewood chips from a pinewood nematode-infected tree, numerous fungal samples were isolated and identified. This study identified five species that have not been previously reported in Korea, namely Geomyces pannorum, Neopestalotiopsis javaensis, Penicillium allii, Penicillium chermesinum, and Ophiognomonia setacea. For all identified species, the cultural features of colonies formed on growth media, their morphological characteristics observed by a light microscope, and their molecular phylogenetic relationships based on nucleotide sequences of the internal transcribed spacer rDNA region or calmodulin gene were described.

약용식물 잎에서 분리한 국내 미기록 진균

안금란 ( Geum Ran Ahn ),노형진 ( Hyeungjin Noh ),이동형 ( Dong Hyeung Lee ),김수산 ( Susan Kim ),김준영 ( Jun Young Kim ),김성환 ( Seong Hwan Kim ) 한국균학회 2019 韓國菌學會誌 Vol.47 No.4

As an effort to explore fungal diversity, fungal survey was undertaken in 2018 in Jangheung, Korea. For the survey, medicinal plant leaves were sampled from Selaginella tamariscina, Serratula coronata ssp. insularis, Scutellaria baicalensis, Scrophularia buergeriana and subjected to fungal isolation. Four unrecorded fungal species, Paraboeremia selaginellae, Colletotrichum camelliae, Alternaria eichhorniae, and Phomopsis capsici were obtained from the survey. This study described their morphological characteristics including colony features formed on media, light microscopic images and molecular characteristics of nucleotide sequences of the ITS and 28S rDNA regions.

모과나무 잔가지에서 분리한 국내 미기록 점균류 Chaenomeles sinesis 보고

안금란 ( Geum Ran Ahn ),김보영 ( Bo Young Kim ),윤여홍 ( Yeo Hong Yun ),손승렬 ( Seung Yeol Son ),김성환 ( Seong Hwan Kim ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.4

Chaenomeles sinensis, called as Chinese quince, belongs to the family Rosaceae and is widely distributed in Korea, China, and Japan. A microorganism was isolated from part of a twig of C. sinensis that showed an abnormal appearance. The microorganism was identified as the slime mold Stemonaria longa of the division Myxomycota, which was previously unrecorded in Korea. The present study reports the morphological characteristics of the isolated fungus and a phylogenetic relationship based on the β-tubulin gene sequences.

원목 및 톱밥배지 버섯 재배사 내 실내 공기서 분리한 미기록 진균 보고

안금란 ( Geum Ran Ahn ),김지은 ( Ji Eun Kim ),김준영 ( Jun Young Kim ),김성환 ( Seong Hwan Kim ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.4

Oak mushroom is cultivated using logs and sawdust media as substrates. In this study, fungi were isolated during a monitoring of indoor air in the oak mushroom cultivation houses located in Cheongyang-gun of Chungnam, Geoje-gun of Gyeongnam, Gumi-si of Gyeongbuk, Jangheung-gun of Jeonnam and Yeoju-si of Gyeongggi-do. Identification of the fungi based on morphology and molecular analysis of the internal transcribed spacer region and 28S rDNA, translation elongation factor translation elongation factor 1 a gene, and β-tubulin gene revealed that six fungi, Cenangium acuum, Neopestalotiopsis surinamensis, Metarhizium marquandii, Periconia macrospinosa, Trichoderma petersenii, and Trichoderma paratroviride that have not been recorded previously in Korea.

경기도 연천 비무장지대 근역에서 분리한 국내 미기록 Eupenicillium, Mortierella, Trichoderma 진균 종 보고

안금란 ( Geum Ran Ahn ),김지은 ( Ji Eun Kim ),오윤석 ( Yun Seok Oh ),이경민 ( Kyoung Min Lee ),진협 ( Hyeop Jin ),김민욱 ( Min Uk Kim ),김준영 ( Jun Young Kim ),김성환 ( Seong Hwan Kim ) 한국균학회 2018 韓國菌學會誌 Vol.46 No.4

As an effort to explore fungal diversity, fungal survey was undertaken in 2017 in the vicinity of demilitarized zone (DMZ) located in Yeoncheon-gun, Gyeonggi-do, Korea. For the survey, wild plants and soils were sampled and subjected to fungal isolation. A total of 18 genera and 23 species including five unrecorded fungal species, Mortierella sclerotiella, M. sossauensis, M. verticillate, Eupenicillium saturniforme, and Trichoderma hispanicum, were obtained from the survey. This study described their morphological characteristics including colony features formed on media, light microscopic images and molecular characteristics of nucleotide sequences of the internal transcribed spacer (ITS) region, 18S and 28S rDNA, β-tubulin gene, calmodulin, and translation elongation factor 1α (tef1α) nDNA genes.

울릉도에서 분리한 미기록 진균 종 보고

안금란,김성환,Ahn, Geum Ran,Kim, Seong Hwan 한국균학회 2015 韓國菌學會誌 Vol.43 No.1

울릉도는 동해에 위치한 화산섬으로 해양성기후이며 생태적으로 다양한 식물들이 존재하는 곳이다. 이 지역에서 진균에 대한 보고가 미진하여 나리분지의 식물과 도동의 흙 시료로부터 진균을 분리하고 형태학적 방법과 분자생물학적 방법으로 동정하였다. 그 결과 C. aleuritis, H. valdunensis, P. uvicola 와 P. caudate가 분리 동정되었다. 이들 진균은 국내에서 처음 보고되는 것이다. Ulleungdo is a volcanic island inhabited by a variety of animals and plants in an oceanic climate in the East Sea. The presence of filamentous fungi has been rarely reported in Ulleungdo. Thus, in this study some plants in the Nari basin and soils in the Dodong region were sampled. Fungi were isolated from the soil and identified by microscopical and molecular biological methods. Coniothyrium aleuritis, Hypocrea valdunensis, Pestalotiopsis uvicola and P. caudate were identified and found to be undescribed species in Korea.

표고 현장적응 시험 버섯 재배사내 공기에서 검출한 국내 미기록 진균 보고

안금란,안홍석,권혁우,고한규,김성환,Ahn, Geum Ran,Ahn, Hong Seok,Kwon, Hyuk Woo,Ko, Han Gyu,Kim, Seong Hwan 한국버섯학회 2016 한국버섯학회지 Vol.14 No.4

Four fungal species, during indoor air monitoring for fungi that possibly affect the field testing of a newly bred shiitake cultivar in cultivation houses located in Cheongyang, Chungnam Province and Jangheung, Jeonnam Province. Of these species, two are known to be plant pathogens and the other two are saprobes but potent contaminators in the mushroom cultivation environment. This study reports the morphological characteristics and phylogenetic relationships of these four species based on nucleotide sequences of the internal transcribed spacer (ITS) and 18S rDNA region, including their known information. 청양과 장흥에 소재한 버섯 재배사에서 새로 육종된 표고 품종의 현장 실증검증 도중 문제를 일으킬 수 있는 잠재적 진균을 파악하고자 재배사내 공기 모니터링을 수행하여 오던 중 국내에 기록이 없는 Mortierella parvispora, Doratomyces purpureofuscus, Periconia byssoides, Periconia pseudobyssoides 등 네 종의 진균을 분리하여 동정하였다. 이중 두 종은 식물병원균으로 알려진 종이었고 다른 두 종은 부생성 균으로 다량의 포자를 생산하고 버섯재배 환경에서 오염균으로 작용할 가능성이 있는 균이었다. 본 연구에서는 이들 동정된 진균에 대한 형태적 특성, internal transcribed spacer (ITS) 와 18S rDNA region 염기서열 분석에 기반한 계통학적 관계, 그리고 알려진 정보 등에 대하여 보고하고자 한다.