보문 : Colletotrichum spp. 유래 scytalone dehydratase 유전자의 유전적 다양성 비교

윤여홍 ( Yeo Hong Yun ),현민우 ( Min Woo Hyun ),서동연 ( Dong Yeon Suh ),김용민 ( Yong Min Kim ),김성환 ( Seong Hwan Kim ),최창원 ( Chang Won Choi ),김영식 ( Young Shick Kim ) 韓國菌學會 2009 韓國菌學會誌 Vol.37 No.2

단보 : 배검은별무늬병균의 Scytalone Dehydratase 멜라닌유전자의 상동성

윤여홍 ( Yeo Hong Yun ),윤성권 ( Seong Kwon Yoon ),손승렬 ( Seung Yeol Son ),김성환 ( Seong Hwan Kim ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.3

일부 자낭균류는 Dihydroxynaphthalene(DHN) 멜라닌을 가지고 있다. 본 연구는 배에 검은별무늬병을 일으키는 V. nashicola균이 어떠한 형태의 멜라닌을 가지고 있는지 확인하고자 DHN 멜라닌 합성유전자중의 하나인 scytalone dehydratase(SD) 유전자의 부분 염기서열을 국내 여러 지역과 일본에서 분리된 11개 균주로부터 분석하였다. PCR 방법을 사용하여 429 bp 크기의 반응산물을 11개 균주 모두로부터 증폭하였고 염기서열을 분석하였다. 증폭된 PCR 산물은 GenBank database에 비교 탐색한 결과 SD 유전자로 판정되었다. 분석된 11개 균주의 SD 유전자에는 모두 1개의 인트론과 122개 아미노산을 코딩하는 2개의 엑손이 존재하였다. 이들 11개 SD 유전자 간에 염기서열은 100%상동성을 보였다. 결정된 V. nashicola SD 유전자의 아미노산 서열의 유사도는 다른 곰팡이와 비교할 때 69~73%로 수준이었다. 본 연구 결과는 V. nashicola균이 DHN 멜라닌 생합성 단계를 운영하고 있음을 입증하였다. Dihydroxynaphthalene (DHN) melanin is known to be present in some ascomycete fungi. To verify the type of melanin in Venturia nashicola that cause scab on pear, we investigated scytalone dehydratase (SD) gene, one of DHN melanin genes, from 11 isolates of V. nashicola from different provinces in Korea and Japan. Through PCR approach, 429 bp amplicon was produced from the 11 isolates and sequenced. All of the PCR-amplified sequences were determined as SD gene through GenBank database search. All the determined sequences were composed of an intron and two exons coding for 122 amino acids of so. The homology of so gene was 100% among the 11 isolates. Sequence identity of the predicted so protein of 122 amino acids ranged 69 to 73% with other fungi. Our results proved that V nashicola operates OHN melanin pathway.

단보 : PCR 기법을 이용한 Phoma glomerate 의 특이검출

윤여홍 ( Yeo Hong Yun ),서동연 ( Dong Yeon Suh ),김현주 ( Hyun Ju Kim ),김성환 ( Seong Hwan Kim ) 한국균학회 2013 韓國菌學會誌 Vol.41 No.1

Phoma glomerata는 식물 잎이나 열매에 병을 일으키는 식물병원균으로 알려져 있다. 국내에서는 아직 피해사례가 없기 때문에 P. glomerata는 국내의 식물검역균으로 관리되고 있다. 본 연구는 국내에 들어오는 목재나 과일에 P.glomerata를 검출할 수 있는 방법 개발코자 수행되었다.Phoma 균주들의 translation elongation factor 1 alpha 유전자 염기서열에 기초하여 P. glomerata 특이적 PCR 프라이머를 디자인 하였고 그 특이성을 검정하였다. PCR 수행결과 P. glomerata에서만 170 bp 크기의 밴드가 증폭되었고, 다른 비교 균주에서는 밴드가 증폭되지 않았다. 검출 감도를 평가하기 위해 기존 PCR방법과 real time PCR방법을 이용하여 실험한 결과 최소 10 pg과 1 pg까지 각각 검출 할 수 있었다. 본 연구결과는 디자인된 PCR 프라이 머가 P. glomerata를 특이적으로 검출하는데 유용 할 것 임을 보여준다. Phoma glomerata (Corda) Wollenw. & Hochapfel is a pathogenic fungus causing spot diseases of plant leaves and fruits. This fungus is important in plant quarantine of seedlings and fruits in Korea. The aim of this study was to develop a sensitive and effective diagnostic method for P. glomerata detection in imported plants. The fungal species-specific PCR primers were designed based on the nucleotide sequences of the translation elongation factor 1 alpha gene and their specificity and sensitivity were tested. The designed primers named as PhoGlo-F and PhoGlo-R amplified specifically a 170 bp sized DNA band of the target gene from the genomic DNA of P. glomerata. No amplicon was produced from genomic DNAs of 16 other Phoma spp. and reference fungal species tested. Moreover, PhoGlo-F/PhoGlo-R primers successfully worked with real-time PCR technique. The detection limit of DNA content by conventional and real-time PCR were 10 pg and 1pg of the genomic DNA of P. glomerata, respectively. We believed that the developed makers would be very useful for P. glomerata detection.

독성화학 물질에 대한 Hydroxides, Oxides, 킬레이트 Cu(tmen)계 화합물의 분해효과

윤여홍 ( Yun Yeo Hong ),최향희 ( Choe Hyang Hui ),정성택 ( Jeong Seong Taeg ),최순자 ( Choe Sun Ja ) 한국공업화학회 2003 공업화학 Vol.14 No.8

독성화학 물질인 sarin, V-agent 및 mustard에 대한 hydroxide계(NaOH, Na₂CO₃, NH₄OH), oxides계(H₂O₂, MMPP, oxone, DCCA), Cu 킬레이트계(Cu(tmen)) 화합물의 농도와 시간에 따른 독성분해 효과를 연구하였다. Hydroxide계 독성분해 물질의 농도범위에 대한 sarin의 분해효율은 NaOH의 효과가 매우 우수한 반면, Na₂CO₃와 NH₄OH의 독성분해 효과는 미미했다. 시간에 따른 분해효율에서 sarin에 대하여 NaOH는 반응초기에 분해효율 92%를 나타낸 반면, V-agent에 대한 NaOH와 sarin에 대한 Na₂CO₃는 반응시간 30 min 이내에 90~100%까지의 증가를 보였다. Sarin에 대한 산화제계 물질의 분해효율은 낮은 반면에 V-agent에 대해서는 비교적 높게 나타났다. 또한 MMPP는 mustard에 대하여 매우 우수한 독성분해 물질로 나타났다. 농도와 시간에 따른 Cu 킬레이트 화합물의 분해효율은 sarin, V-agent, 그리고 mustard에 대해 전반적으로 미약했다. 결국 sarin은 NaOH, V-agent는 MMPP와 DCCA, 그리고 mustard는 MMPP로 독성분해 효율이 크게 나타나 기존에 사용되던 STB나 DS-2를 대체할 수 있었으며, 대부분의 독성분해는 반응초기에 이루어지고 있음이 밝혀졌다. The resolving effect of hydroxides (NaOH, Na_(2)CO_(3), NH_(4)OH), oxides (H_(2)O_(2), Oxone and DCCA) and Cu-chelate (Cu(tmen)) series of compounds on the toxic chemicals, such as sarin, V-agent and mustard were investigated in terms of the concentration and reaction time of the decontamination materials. The toxicity resolving effect of NaOH along the concentration variance on sarin exhibited superior, while that effect of the rest of the materials exhibited poor. According to the reaction time, the resolving effect of NaOH on sarin reached to 92% in the beginning of the reaction, while the effect of NaOH on V-agent and that of Na_(2)CO_(3) on sarin gradually increased up to 90∼100% within 30 min. The resolving effect of all oxides compounds on sarin exhibits negligible, but that on V-agent is relatively high. In particular, MMPP is one of the most effective agents for mustard. Regarding on the reaction time, the most effective reaction is accomplished within few minutes. The resolving effect of Cu-chelate compounds along the concentration and the reaction time on sarin, V-agent, and mustard is all relatively negligible. Thus. the best decontamination chemical agents are NaOH and MMPP for sarin and mustard, respectively, whereas MMPP and DCCA are the best for V-agent. Thus STB and DS-2 of which the commercial decontamination agents, may be replaced by NaOH, MMPP, or DCCA for resolving toxic chemicals. In addition, the resolving effect of the above toxicity resolving effect occurred immediately as the two materials reacted.

표고 재배사 실내 공기에서 분리한 국내 미기록 진균

권혁우,윤여홍,김준영,김성환,고한규,Kwon, Hyuk Woo,Yun, Yeo Hong,Kim, Jun Young,Kim, Seong Hwan,Ko, Han Kyu 한국균학회 2015 韓國菌學會誌 Vol.43 No.1

진균에 의한 오염은 재배사에서 톱밥 배지를 이용한 표고 재배에 중대한 영향을 미치기 때문에 표고 재배사 실내 공기에 존재하는 진균에 대한 모니터링을 수행하던 중 여러 진균을 분리하였다. 분리된 진균 중에는 국내 미기록인 Aspergillus pulverulentus와 Cosmospora butyri 등 2종을 비롯하여 기록은 있으나 균학적 확증이 부족한 Lecanicillium psalliotae와 L. antillanum 등 2종이 존재하였다. 본 논문에서는 이들 균류에 대한 형태적 특성과 더불어 internal transcribed spacer (ITS) rDNA region 또는 ${\beta}$-tubulin 유전자 염기서열에 기반한 계통학적 분석 결과를 기술하였다. Mold contamination is one of the detrimental factors affecting sawdust media-based shiitake cultivation in greenhouses. During mold monitoring of indoor air of greenhouses, several fungi were isolated. Among them, Aspergillus pulverulentus and Cosmospora butyri were found to be new in Korea and Lecanicillium psalliotae and L. antillanum were known in Korea without taxonomic validation. In this study the morphological characteristics and phylogenetic analysis based on the internal transcribed spacer (ITS) rDNA region or ${\beta}$-tubulin gene of the four identified species were described.

경계성 난소종양 수술 시 우연히 발견한 충수돌기 유암

문윤진 ( Mun Yun Jin ),정세민 ( Jeong Se Min ),박승근 ( Park Seung Geun ),김민정 ( Kim Min Jeong ),백소야 ( Baeg So Ya ),박혜영 ( Park Hye Yeong ),윤여홍 ( Yun Yeo Hong ),지용헌 ( Ji Yong Heon ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.3

A case with carcinoid tumor of the appendix was encountered incidentally during an elective abdominal surgery for borderline mucinous ovarian tumor in a 16-year old female. Carcinoid tumor of appendix is the most common neoplasm of the gastrointestinal tract in childhood and adolesence. They are usually regarded as a benign neoplasm and an uncommon source of metastasis and recurrence. We report this case with reference to the value of grosss examination of the appendix and incidental appendectomy during gynecologic abdominal operations.

단보 : 표고 재배사 실내 공기에서 분리한 국내 미기록 진균

권혁우 ( Hyuk Woo Kwon ),윤여홍 ( Yeo Hong Yun ),김준영 ( Jun Young Kim ),김성환 ( Seong Hwan Kim ),고한규 ( Han Kyu Ko ) 한국균학회 2015 Mycobiology Vol.43 No.1

진균에 의한 오염은 재배사에서 톱밥 배지를 이용한 표고 재배에 중대한 영향을 미치기 때문에 표고 재배사 실내공기에 존재하는 진균에 대한 모니터링을 수행하던 중 여러 진균을 분리하였다. 분리된 진균 중에는 국내 미기록인 Aspergillus pulverulentus와 Cosmospora butyri 등 2종을 비롯하여 기록은 있으나 균학적 확증이 부족한 Lecanicillium psalliotae와 L. antillanum 등 2종이 존재하였다. 본 논문에서는 이들 균류에 대한 형태적 특성과 더불어 internal transcribed spacer (ITS) rDNA region 또는 β-tubulin 유전자 염기서열에 기반한 계통학적 분석 결과를 기술하였다. Mold contamination is one of the detrimental factors affecting sawdust media-based shiitake cultivation in greenhouses. During mold monitoring of indoor air of greenhouses, several fungi were isolated. Among them, Aspergillus pulverulentus and Cosmospora butyri were found to be new in Korea and Lecanicillium psalliotae and L. antillanum were known in Korea without taxonomic validation. In this study the morphological characteristics and phylogenetic analysis based on the internal transcribed spacer (ITS) rDNA region or β-tubulin gene of the four identified species were described.

식물인간 상태의 임신 관리 및 분만 1예

조지연(Ji Yeon Cho),윤여홍(Yeo Hong Yun),정세민(Sae Min Chung),서상용(Sang Yong Seo),지용헌(Yong Hun Chee) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.2

In the management of the traumatized gravid and her fetus, diagnostic tests and therapy should be directed primarily at the care of the mother and should not be delayed or compromised because of the pregnancy. Because many factors can damage when the mother is unconscious, fetal well-being should be assessed by serial biophysical profile scoring. If the mother's condition is stable as in the chronic vegetative state, elective delivery might best be performed at a gestational age of 32 to 34 weeks.

보문 : 진균과 식물의 Phenylalanine Ammonia-Lyase 그리고 세균의 Histidine Ammonia-Lyase 간의 면역학적 관계 분석

현민우 ( Min Woo Hyun ),윤여홍 ( Yeo Hong Yun ),서동연 ( Dong Yeon Suh ),한지혜 ( Ji Hae Han ),김성환 ( Seong Hwan Kim ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3

Phenylalanine ammonia-lyase (PAL) from the maize pathogen Ustilago maydis was analysed immunologically to obtain insights into the structural relationships between plant PAL and fungal PAL and between PAL and histidine ammonia-lyase (HAL). Cross-reactivity was found among all the PAL proteins from different species tested, using antibodies raised against both plant and fungal PALs. Both anti-Alfalfa and anti-popular PAL antibodies strongly recognized plant PALs but only weakly recognized fungal PALs. Antibodies raised against U. maydis PAL only weakly recognized the Rhodotorula glutinis yeast PAL. The anti-U. maydis PAL antibodies showed low affinity for the plant PALs but they bound strongly to Pseudomonas bacterial HAL. Significant cross-reactivity between the two plant PAL antibodies and the bacterial HAL was also observed. Both the anti-Ustilago PAL and the anti-poplar PAL antibodies displayed similar enzyme inhibition patterns, including moderate inhibition of bacterial HAL activity. However, the bacterial HAL antibody inhibited only Ustilago PAL. The PAL and HAL antibodies tested showed no inhibition against yeast PAL. This is first report on the immunological relationships between PAL and HAL.

복식 전 자궁 적출술 후 대퇴신경 장애

정세민 ( Chung Sae Min ),문윤진 ( Moon Yun Jin ),박승근 ( Park Seung Geun ),박혜영 ( Park Hye Yeong ),조지연 ( Cho Ji Yeon ),윤여홍 ( Yun Yeo Hong ),지용헌 ( Chee Yong Hun ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.7

Total abdominal hystrectomy is the most common surgery of Gynecology. It`s complication are taken very important. Although neuropathy, especially femoral nerve injury, is rare, recently we have experienced a case of femoral neuropathy after total abdominal hystrectomy. We present this case with a brief review of literature.