인삼 사포닌이 일산화탄소중독 및 노화과정에서 생쥐의 뇌신경세포 분포에 미치는 영향

신정희(Jeung Hee Shin),이인란(Ihn Rhan Lee),조금희(Geum Hee Cho),윤재순(Jae Soon Yun) 대한약학회 1992 약학회지 Vol.36 No.3

The effects of ginseng saponins on the distribution of nerve cells in cerebral cortex of carbon monoxide (CO)-intoxicated mice were studied in the young (5~8 weeks) and aged (43~52 weeks) mice. Mice were exposed to 5000ppm of CO for 40 minutes (72% HbCO). After that, nerve cells in motor(area 4), somatosensory(area 3) and visual(area 17) area of cerebral cortex was observed. In young mice, the number of nerve cells in each area was significantly decreased on 1st, 7th and 14th day after CO intoxication. In aged mice, that was also decreased after CO intoxication. Especially the number of the nerve cells in motor and somatosensory area was significantly decreased on lst and 7th day, while that in visual area was decreased only on 1st day. The number of nerve cells in young mice pretreated with ginseng saponins were significantly decreased less on 7th and 14th day than that of untreated mice. The number of nerve cells in each area of normal aged mice was larger than that of normal young mice. The results suggest that CO exposure causes local degeneration or disturbance of nerve cells and delayed neurologic sequelae, while ginseng saponins might play a role of protective action on the nerve cells which were damaged by CO.

인삼 사포닌이 일산화탄소중독 및 노화과정에서 흰쥐의 뇌에너지 대사물 함량 변화에 미치는 영향

신정희(Jeung Hee Shin),최현진(Hyung Jin Choi),강지원(Ji Won Kang),박혜영(Hea Young Park),윤재순(Jae Soon Yun) 大韓藥學會 1992 약학회지 Vol.36 No.3

This study was performed to investigate the effects of ginseng saponins on the cerebral energy metabolite''s contents influenced by carbon monoxide(CO) intoxication. Each experimental group was divided into young (5~8 weeks) and aged (43~52 weeks) rats, and they were exposed to 5,000 ppm CO (72% HbCO) for 30 min. One of the other groups was pretreated with ginseng saponins for 5 days before CO intoxication. The contents of cerebral energy metabolites in cerebral cortex, stratum and hypothalamus were measured. In cerebral cortex of both young and aged rats, the levels of ATP and creatine phosphate were significantly decreased, while those of lactate were significantly increased. There was no difference between the levels of cerebral energy metabolites of young and aged rats. Pretreatment of ginseng saponins before CO intoxication lowered decrease of the levels of cerebral energy metabolites and ATP levels were significantly recovered. On the other hands, contents of lactate in stratum and hypothalamus of young rats were significantly increased and the levels of ATP and creatine phosphate in stratum and hypothalamus were completely recovered at 2 weeks after CO intoxication. The results suggest that ginseng saponins have an ameliorating action against disturbance of the cerebral energy metabolites by CO intoxication.

인삼 사포닌이 일산화탄소와 노화에 의한 신경전달계 변화에 미치는 영향

윤혜정(Hae Chung Yun),신정희(Jeung Hee Shin),최현진(Hyun Jin Choi),윤재순(Jae Soo Yun) 대한약학회 1992 약학회지 Vol.36 No.1

The present study examined the effects of carbon monoxide (CO) intoxication and aging on learning and memory deficit in young (5~8 weeks) and aged (52~66 weeks) mice, using the step down and step through passive avoidance failure techniques. We also investigated the effects of ginseng saponins on memory deficit Significant decrease in memory registration, retention and retrieval function in young mice and decrease in memory registration and retention function in aged mice were observed. Normal young mice were apt to perform to a great degree of passive avoidance response than normal aged mice, but there was no difference between both groups by CO exposure, Administration of ginseng saponins showed an improvement on passive avoidance failure induced by CO exposure.

은행잎 추출성분이 고혈압쥐의 뇌중 신경전달물질 함량에 미치는 영향

박혜영(Hea Young Pchoo),주지연(Ji Yeon Ju),김명희(Myung Hee Kim),신정희(Jeung Hee Shin) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.1

Effects of Ginkgo biloba leaf extract (GBE) and its components kaempferol-coumaroyl glucosyl rhamnoside (Kc), quercetin-coumaroyl glucosyl rhamnoside (Qc), ginkolide (G) and bilobalide (B) on blood pressure and on the levels of neurotransmitters in the spontaneously hypertensive rat brain were examined. Blood pressure decreased about 10 mmHg after administration of GBE but without significance. The concentration of norepinephrine increased (3 times) and that of DOPAC was decreased (38%) after administration of Qc. And 3-MT concentration was increased (36%) by Kc administration in striatum. While Qc administration increased the concentration of Ach (260%) and Ch(29%) significantly, B administration increased Ch concentration in cerebral cortex.

Eicosanoid 유도체가 흰쥐 혈압 변화에 미치는 영향

윤재순(Jae S . Yun),윤연숙(Yeon S . Yun),신정희(Jeung Hee Shin),최현진(Hyun J Choi),최진아(Jin A Choi) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.2

Arachidonic acid (AA, C20 : 4, ω-6) and eicosapentanoic acid (EPA,C20: 5, ω-3), which are polyunsaturated fatty acids forming eicosanoids, were tested for their effects on blood pressure in Wistar rats and SHR. AA is the most important precursor for the biosynthesis of eicosanoids which include the prostaglandins, prostacyclin (PGI₂), thromboxane A₂ (TXA₂) and the leukotriens. TXA₂ is a potent vasoconstrictor and a powerful inducer of platelet aggregation causing myocardial infarction and hypertention. In contrast, PGI₂ induces vasodilation and inhibits platelet aggregation. In this study, AA markedly increased blood pressure, but its effect was antagonized by both EPA, a structural analog of AA, and dazmegrel, a TX synthetase inhibitor. Also, AA enhanced the antihypertensive effects of hydralazine and captopril, and EPA reduced TXA₂ production. These results indicate that the hypotensive effects of EPA might be closely related to the decrease in TXA-2 biosynthesis due to competitive inhibition by structural similarity of the EPA to the AA, the precursor of TXA₂.

인삼 사포닌이 일산화탄소중독 및 노화과정에서 흰쥐의 뇌에너지 대사물 함량 변화에 미치는 영향

신정희,최현진,강지원,박혜영,윤재순 梨花女子大學校 藥學硏究所 1992 藥學硏究論文集 Vol.- No.2

This study was performed to investigate the effects of ginseng saponins on the cerebral energy metalbolite's contents influenced by carbon monoxide(CO) intoxication. Each experimental group was divided young (5~8 weeks) and aged (43~52 weeks) rats, and they were exposed at 5,000 ppm CO(72% HbCO) for 30 min. One of the other groups was pretreated with ginseng saponins for 5 days before CO intoxication. The contents of cerebral energy metabolites in cerebral cortex, stratum and hypothalamus were measured. In cerebral cortex of both young and aged rats, the levels of ATP and creatine phosphate were significantly decreased, while those of lactate were significantly increased. There was no difference between the levels of cerebral energy metabolites of young and aged rats. Pretreatment of ginseng saponins before CO intoxication lowered decrease of the levels of cerebral energy metabolites and ATP levels were significantly recovered. On the other hands, contents of lactate in stratum and hypothalamus of young rats were significantly increased and the levels of ATP and creatine phosphate in stratum and hypothalamus were completely recovered at 2 weeks after CO intoxication. The results suggest that ginseng saponins have an ameliorating action against disturbance of the cerebral energy metabolites by CO intoxication.

인삼 사포닌이 일산화탄소중독 및 노화과정에서 생쥐의 뇌신경세포 분포에 미치는 영향

신정희,이인란,조금희,윤재순 梨花女子大學校 藥學硏究所 1992 藥學硏究論文集 Vol.- No.2

The effects of ginseng saponins on the distribution of nerve cells in cerebral cortex of carbon monoxide (CO)-intoxicated mice were studied in the young (5~8 weeks) and aged (43~52 weeks) mice. Mice were exposed to 5000ppm of CO for 40 minutes (72% HbCO). After that, nerve cells in motor(area 4), somatosensory(area 3) and visual(area 17) area of cerebral cortex was observed. In young mice, the number of nerve cells in each area was significantly decreased on 1st, 7th and 14th day after CO intoxication. In aged mice, that was also decreased after CO intoxication. Especially the number of the nerve cells in motor and somatosensory area was significantly decreased on 1st and 7th day, while that in visual area was decreased only on 1st day. The number of nerve cells in young mice pretreated with ginseng saponins were significantly decreased less on 7th and 14th day than that of untreated mice. The number of nerve cells in each area of normal aged mice was larger than that of normal young mice. The results suggest that CO exposure causes local degeneration or disturbance of nerve cells and delayed neurologic sequelae, while ginseng saponins might play a role of protective action on the nerve cells which were damaged by CO.

인삼 사포닌이 일산화탄소중독 및 노화과정에서 생쥐의 뇌신경세포 분포에 미치는 영향

신정희,이인란,조금희,윤재순 이화여자대학교 생명과학연구소 1992 생명과학연구논문집 Vol.3 No.-

The effects of ginseng saponins on the distribution of nerve cells in cerebral cortex of carbon monoxide (CO)-intoxicated mice were studied in the young (5~8 weeks) and aged (43~52weeks) mice. Mice were exposed to 5000 ppm of CO for 40 minutes (72%HbCO). After that, nerve cells in motor(area 4), somatosensory(area 3) and visual ((area 17) area of cerebral cortex was observed. In young mice, the number of nerve cells in each area was significantly decreased on 1st. 7th and 14th day after CO intoxication. In aged mice, that was also decreased after CO intoxication. Especially the number of the nerve cells in motor and somatosensory area was significantly decreased on 1st and 7th day, while that in visual area was decreased only on 1st day. The number of nerve cells in young mice pretreated with ginseng sapoinins were significantly decreased less on 7th and 14th day than that of untreated mice. The number of nerve cells in each area of normal aged mice was larger than that of normal young micel. The resultssuggest that CO exposure causes local degeneration or disturbance of nerve cells and delayed neurologic sequelae, while ginseng saponins might play a role of protetive action on the nerve cells which were damaged by CO.

Piracetam이 마우스의 기억력 및 대뇌피질내 Acetylcholine량 변화에 미치는 영향

尹再順,申精姬,李容筍,劉永瓚 梨花女子大學校 韓國生活科學硏究院 1991 韓國生活科學硏究院 論叢 Vol.47 No.-

The central cholinergic synapse is known to be closely connected with memory. Previously, piracetam has been reported to improve the intellectual ability. We used a scopolamine-induced amnesiac mouse to study the effects of piracetam on memory retrieval by passive avoidance learning test and on the acetylcholine level of cerebral cortex. The amnesia was produced in mouse by the intraperitoneal injection(i.p.) of scopolamine at a dose of 1 ㎎/㎏ body weight(b.w.). Upon administration of piracetam to amnesiac mouse at doses of 100 and 200 ㎎/㎏ b.w., i.p., 30 min, prior to retrieval test, the significant improvement on memory impairment was observed by examination 24 hr after training. The level of acetylcholine in cerebral cortex of mouse was decreased by the scopolamine treatment, however, this decrease was attenuated by piracetam. These results suggest that the effect of piracetam on memory may be attributed to activation of cholinergic nerve.

인삼 사포닌이 일산화탄소와 노화에 의한 신경전달계 변화에 미치는 영향 : 기억력 장해에 미치는 영향 Effect on the Memory Impairment

윤혜정,신정희,최현진,윤재순 梨花女子大學校 藥學硏究所 1992 藥學硏究論文集 Vol.- No.2

The present study examined the effects of carbon monoxide (CO) intoxication and aging on learning and memory deficit in young(5~8 weeks) and aged (52~66 weeks) mice, using the step down and step through passive avoidance failure techniques. We also investigated the effects of ginseng saponins on memory deficit. Significant decrease in memory registration, retention and retrieval function in young mice and decrease in memory registration and retention function in aged mice were observed. Normal young mice were apt to perform to a great degree of passive avoidance response than normal aged mice, but there was no difference between both groups by CO exposure. Administration of ginseng saponins showed an improvement on passive avoidance failure induced by CO exposure.