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한우(韓牛)의 증식저해(增殖沮害) 요인(要因)에 관한 연구(硏究) -기생충학적(寄生蟲學的) 및 의화학적(醫化學的) 조사(調査)-
장두환,신재두,Jang, Du Hwan,Shin, Jae Doo 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.2
Parasitological and biochemical studies for 82 heads (12.2%) showing sterility syndrom out of 672 heads of Korean native cattle were undertaken by analysis of blood values for their contents and examination of their vaginal swabs for Tritrichomonas foetus 1. Tritrichomonas infection of Korean cattle revealed 7 positive cases (8.54%) out of 82 infertile heads. 2. Contents of their serum protean, carotenoid, vitamin A and phosphorus were determined in summer and winter season and the following results were obtained. a) In summer season, average blood value of 30 cattle showing sterility syndrom were determined as protein 7.52g/100ml, carotenoid 165.1g/100ml, vitamin A 173.9 IU/100ml and phosphorus 7.48mg/100ml, respectively. b) In winter. season, average blood value of 52 cattle showing sterility syndrom were determined as protein 7.76g/100ml, carotenoid 413.3g/100ml, vitamin A 174,3 IU/100ml and phosphorus 7.54mg/100ml, respectively.
Aspergillus niger 가 생산하는 섬유소 분해효소의 정제 및 특성
박관화,오태광,신재두 한국농화학회 1981 Applied Biological Chemistry (Appl Biol Chem) Vol.24 No.3
Three fractions of carboxymethyl-cellulase (F-Ⅰ, F-Ⅱ, and F-Ⅲ) and β-glucosidase form Aspergillus uiger were partially purified by ammonium sulfare fractionation. Sephadex G-150 and DEAE Sephadex column chromatography. The optimum conditions such as pH and temperature and thermal inactivation properties of the enzymes were investigated. Arrhenius plots of F-Ⅱ and F-Ⅲ appeared as straight lines, whereas that of F-Ⅰ was biphasic. The Z-values of F-Ⅱ and F-Ⅲ were 8℃ and 10℃ respectively, while that of F-I was 4℃ over 60∼70℃ and 383℃ over 70∼98℃. Three fractions and the crude extract of carboxymethyl-cellulase exhibited a similar optimum pH 4.3 and temperature of 60℃, while Z-value of crude activity of both purified and crude extract of β-glucosidase was shown at pH 4.7 and 60℃, and z-value of the enzyme was 7℃.