농업부산물의 이용성향상에 관한 연구 : 제3보 탈지미강(脫脂米糠)부터 phytin 분리 Park 3. Separation of phytin from defatted rice bran

신재두 한국농화학회 1968 Applied Biological Chemistry (Appl Biol Chem) Vol.9 No.1

Defatted rice bran is mixed with diluted acid solution, the mixture is agitated some hrs. at constant temparature. After the mixture is filtered, thus filtrate is obtained. This filtrate is phytin extract solution. (Test-1) The alkali is added to this filtrate and filtered out, then the precipitation of phytin is obtained. (Test-2) At the test-1, the effect of kind of acid, conc. of acid, amount of extract sol'n., time of extraction, temp. of extraction, to the extract amount of phytin is tested. Consequently, the following facts are known. 1. Amount of phytin extract is greater HCI extraction than H₂SO₄ extraction. 2. At 0.3% HCI, the amount of phytin extract is greatest of all HCl extraction. 3. The sufficient amount of acid solution is 8-10 times of amount of defatted rice bran. 4. The time of extraction at room temperature is sufficient 8-12 hrs. 5. When extract temperature is 20-30℃, the amount of phytin extraction is greater of all temp.. 6, When defatted rice bran 20 g is shanken with 160 ㎖ of 0.3% HCl for 10 hr. at room temp., in this case the amount of phytin extract is 11.34% of defatted rice bran, it is 93% of theoretical yield. At the test-2 the effect of kind of precipitation agent, degree of nutralization to the amount of phytin prc,ipitation is tested. 1. Degree of nut. is best at pH 6.8-7.0. 2. When use of Ca(OH)₂ the amount of phytin precipitation is more than use of KOH, NaOH, or NH₄OH. 3. At pH 6.0-7.2, the solubility of phytin is followed. K-phytate〉 NH₄-phytate〉 Na-phytate〉 Ca-phytate 4. When phytin extract solution is nutralized with Ca(OH)₂ to pH 7.0, the amount of phytin precipitation is 94.78% of theoretical yield.

農業副産物의 利用性向上에 關한 硏究 : 第1報 棉實殼부터 Furfural 製造

申載斗 서울대학교 1966 서울대학교 論文集 Vol.17 No.-

1. Lot of agricultural waste products for example rice bran and hull which is contained many useful substances, vainly have been wasted or burned as a low efficiency fuel. Among many agricultural waste products auther has been examined to liberate the furfural especially from cotton seed hull. 2. Furfural is one of a heterocyclic aldehyde which is obtained from agricultural waste praducts(cotton seed hull) and mineral acid. 3. Preparation of Furfural A: Dilute acid method; cottonseed hull is reacted with many amount dilute acid. B. Concentrated acid method with small amount of 1% acid. 4. A: Cotton seed hull I heated with dilute sulfuric acid several times of hull in autoclave under the pressure and the gasis liberated, after then distrilate is cooled.

Tannin extract의 製造에 관한 硏究 : 도토리묵 廢水에서 Tannin 回收

申載斗 세계평화교수협의회 1976 아카데미論叢 Vol.4 No.1

도토리 및 상수리 등에서 Tannin을 침출한 희석용액으로부터 Tannin extract를 경제적으로 제조할 목적으로 실험한 결과는 1. 원료에서 Tannin을 침출할 때 원료분쇄S물을 상온에서 그 8배량의 물로 90분씩 3회 침출하면 원료중의 Tannin을 거의 전부 침출시킬 수 있다. 2. 침출액에서 불용성 Tannin산염을 침전시킬 때 침전제로서 연화합물을 사용함이 좋다. 3. 원료로부터 Tannin extract를 약 9% 수득할 수 있다. Tannin수율은 원료의 90% 정도이다. 4. 본 연구로 제조된 Tannin extract의 품질은 시판품보다 좋다.

農産副産物의 利用性向上에 關한 硏究 : 第2報 韓國産米糠中의 Phytin에 關한 硏究-1 Part 2. Studies of the Phytin of the rice bran produced in Korea.

申載斗 서울대학교 1967 서울대학교 論文集 Vol.18 No.-

Studies on contents and molecular formular of the phytin of the defatted rice bran produced in each districts of Korea were performed. And the new method of phytin determination was resulted through the performed experiments. Phytin contenes of rice bran showed average 13 to 14 percentage. The molecular formular of Ammonim Phytin of rice bran, which produced in Janghang of cungnam, Junjoo of Junbuk, Taegoo of Kyongbuk province, was found to be C_6H_8O_24P_6Mg_4Ca(NH_4)_2 5H_O, and that, which was produced in Taejon of chungnam, Suwon of Kyongi, and Kwangjoo of Junnam province, was found to be C_6H_8O_24P_6Mg_3Ca_2(NH_4)_2 5H_2O, which had one less Mg atom one more Ca atom. The method of phytin determination studied through this experiments seem to be accurate. To a 100 milliters of the hydrochloric acid solution regulated 0.5 to 0.1 percentage, containing 0.01 to 0. percentage of phytin, add 10 milliters of 0.2% potassium thiocyanate solution, and 1 milliters of 3% hydrogen perxide solution, then titrate in a short minuites the phytin with standard 0.5% ferric chloride N-HCl solution the cotents boiling. 1.574mg Fe corresponds to 0.1g Phytin.

毛髮에서 L-Cystine의 分離

申載斗 서울大學校 農科大學 1983 서울대농학연구지 Vol.8 No.1

Human hair was hydrolyzed with boiling HCI solution (30%) for five hours. The hydrolyzate was neutralized with Na₂CO₃ solution up to pH 4.6 and filtered. The filtrate was stored in a cool place for three days to obtain the crude crystal of L-cystine. The yield was 91.8% in terms of recovery. The crude cystine was dissolved in the two per cent hydrochloic acid the solution was treated with active carbon. The filtrate was neutralized with the ten per cent aqueous solution of ammonia up to pH 4.2 and the purified crystal of L-cystine was obtained after cooling. Purity of the cystine wad 99.3% and the recovery 98.33%. The recovery of L-cystine from human hair containing 15.42% cystine and 3.3% tyrosine was 77.8%.

施肥에 따른 水稻의 Phytin成分 硏究

申載斗 서울大學校 農科大學 1981 서울대농학연구지 Vol.6 No.1

The defatted rice bran was obtained from rice plant grown in the rice field which dressed with slaked lime and straw. The amount of P, Ca, Mg and phytin in the defatted rice bran were anylyzed and the refined Na-phytate which were obtained from rice bran were also determined. The results obtained can be summarized as follows. 1. The amount of P, Ca and Mg are little difference were appeared between the soil of treated area and its control. The average value of Ca/Mg in the soil of both areas was 100/38. 2. The contents of P, Ca and Mg in the defatted rice bran which were obtained from rice plants were 4.06∼4.15%, 1.10∼1.17 and 1.19∼1.28% respectively and the average value of Ca/Mg in the rice bran was 100/111.5. The phytin content of the rice bran was shown 14.28% in average and the molecular formular of Na-phytin content of the rice bran was found to be C?H?O?P?Mg₄CaNa·5H₂O. 3. The content of phytic Ca+Mg was 88.2% of total Ca+Mg in the rice bran.

平和의 廣場에서

申載斗 세계평화교수협의회 1977 廣場 Vol.45 No.1

韓國産 미간의 Phytin 硏究

申載斗 세계평화교수협의회 1980 廣場 Vol.81 No.-

나의 存在

申載斗 세계평화교수협의회 1981 廣場 Vol.98 No.-

Chelate 법에 의한 Phytin 분석에 관한 연구

신재두 한국농화학회 1968 Applied Biological Chemistry (Appl Biol Chem) Vol.10 No.1

Phytin is a salt(mainly calcium and magnesium) of phytic acid and its purity and molecular formula can be determined by assaying the contents of phosporus, calcium and magnesium in phytin. In order to devise a new method for the quantitative analysis of the three elements in phytin, the chelatometric method was developed as follows: 1) As the pretreatment for phytin analysis, it was ashfied st 550∼600℃ in the presence of concentrated nitric acid. This dry process is more accurate than the wet process. 2) Phosphorus, calcium and megnesium were analyzed by the conventional and the new method described here, for the phytin sample decomposed by the dry process. The ashfied phytin solution in hydrochloric acid was partitioned into cation and anion fractions by means of a ration exchange resin. A portion of the ration fraction was adjusted to pH 7.0 followed by readjustment to pH 10 and titrated with standard EDTA solution using the BT [Eriochrome black T] indicator to obtain the combined value of calcium and magnesium. Another portion of the ration fraction was made to pH 7.0, and a small volume of standard EDTA solution was added to it. pH was adjusted to 12∼13 with 8 N KOH and it was titrate by a standard EDTA solution in the presence of N-N[2-Hydroxy-1-(2-hydroxy-4-sulfo-1-naphytate)-3-naphthoic acid] diluted powder indicator in order to obtain the calcium content. Magnesium content was calculated from the difference between the two values. From the anion fraction the magnesium ammonium phosphate precipitate was obtained. The precipitate was dissolved in hydrochloric acid, and a standard EDTA solution was added to it. The solution was adjusted to pH 7.0 and then readjusted to pH 10.0 by a buffer solution and titrated with a standard magnesium sulfate solution in the presence of BT indicator to obtain the phosphorus content. The analytical data for phosphorus, calcium and magnesium were 98.9%, 97.1% and 99.1% respectively, in reference to the theoretical values for the formula C_6H_6O_(24)P_6Mg₄CaNa₂·5H₂O. Statical analysis indicated a good coincidence of the theoretical and experimental values. On the other hand, the observed values for the three elements by the conventional method were 92.4%, 86.8% and 93.8%, respectively, revealing a remarkable difference from the theoretical. 3) When sodium phytate was admixed with starch and subjected to the analysis of phosphorus, calcium and magnesium by the chelatometric method, their recovery was almost 100% 4) In order to confirm the accuracy of this method, phytic acid was reacted with calcium chloride and magnesium chloride in the molar ratio of phytic: calcium chloride: magnesium chloride=1 : 5 : 20 to obtain sodium phytate containing one calcium atom and four magnesium atoms per molecule of sodium phytate. The analytical data for phosporus, calcium and magnesium were coincident with those as determine d by the aforementioned method. The new method employing the dry process, ion exchange resin and chelatometric assay of phosphorus, calcium and magnesium is considered accurate and rapid for the determination of phytin.