일반연제 발표 : 종양학 2, 일반부인과학 ; 원발성 질암 21례에 대한 임상적 고찰

신승화,박은동 대한산부인과학회 1994 대한산부인과학회 학술대회 Vol.74 No.-

인간 대장암 세포주에서 sulindac sulfide 처리에 의해 차별적으로 발현되는 유전자 군의 분석

신승화,김종식,Shin, Seung-Hwa,Kim, Jong-Sik 한국생명과학회 2007 생명과학회지 Vol.17 No.7

본 연구에서는 NSAID계 약물인 sulindac, sulindac sulfone, 그리고 sulindac sulfide 처리에 의한 암세포 생존율에 미치는 영향을 확인하기 위하여, 인간 대장암 세포주인 HCTl16에 각각 10 ${\mu}M$의 NSAID들을 처리하였다. 처리한약물 중 sulindac sulfide에 의한 암세포 생존율이 가장 높게 감소하는 것으로 MTS assay 결과 확인되었다. 또한 sulindac sulfide의 처리 농도가 증가됨에 따라 세포 생존율이 감소하는 것으로 확인되었다. Sulindac sulfide의 처리에 따른 이러한 암 세포 사멸의 분자생물학적 기전을 이해하기 위하여, oligo DNA microarray 실험을 수행하였다. 그 결과, 10 ${\mu}M$의 sulindac sulfide의 처리에 의해 2배 이상 발현이 증가되는 유전자가 23개 확인되었고, 반대로 2배 이상 발현이 감소되는 유전자가 33개 확인되었다. 증가되는 유전자중 3개(NAG-1, DDIT3, PCK2)를 선택하여, RT-PCR과 real-time PCR을 수행하였다. 그 결과 두 실험 모두 DNA microarray 실험결과와 동일하게 발현이 증가되었다. 이 중 sulindac, sulindac sulfone, sulindac sulfide에 의 한 NAG-1 유전자의 발현변화를 RT-PCR과 real-time PCR 방법으로 확인한 결과, sulindac sulfide에 의한 암 억제유전자인 NAG-1의 발현이 가장 많이 발현되었다. 이러한 연구결과는 세포생존율 결과와 비교하였을 때, NAG-1의 높은 발현과 암 세포 생존율의 감소가 관련이 있음을 간접적으로 시사한다. 따라서 이들 연구결과는 sulindac sulfide에 의한 화학적 암 예방법의 분자생물학적 기전을 이해하는데 도움을 줄 것으로 생각한다. To investigate whether sulindac, sulindac sulfone, and sulindac sulfide could affect cancer cell viabilities, human colorectal HCTl16 cells were treated with 10 ${\mu}M$ of each NSAID. Among treated NSAms, sulindac sulfide dramatically decreased the cell viabilities detected by MTS and the cytotoxic effect showed dose-dependent manner. To understand the molecular mechanism of cell death in response to sulindac sulfide treatment, we performed oligo DNA microarray analysis. We found that 23 genes were up-regulated more than 2 folds, whereas 33 genes were down-regulated more than 2 folds by treatment of 10 ${\mu}M$ sulindac sulfide. Among the up-regulated genes, we selected 3 genes (NAG-1, DDIT3, PCK2) and performed RT-PCR and quantitative real-time PCR to cofirm microarray data. The results of RT-PCR and real-time PCR were highly accorded with those of microarray experiment. As NAG-1 is well-known gene as tumor suppressor, we detected changes of NAG-1 expression by 10 ${\mu}M$ of sulindac, sulindac sulfone, and sulindac sulfide. The results of RT-PCR and quantitacve real-time PCR indicated that sulindac sulfide was the strongest inducer of NAG-1 among treated NSAIDS. This result implies that induction of NAG-1 by sulindac sulfide plays important role in cell death of colorectal cancer. Overall, we speculate that these results may be helpful in understanding the molecular mechanism of the cancer chemoprevention by sulindac sulfide in human colorectal cancer.

Sequential protein delivery using a microsphere/hydrogel combination system for therapeutic angiogenesis

신승화,이장욱,임광석,이상경,김용희,이근용 한국고분자학회 2011 한국고분자학회 학술대회 연구논문 초록집 Vol.2011 No.1

한국어판 피츠버그 수면의 질 지수(PSQI-K) 도구의 신뢰도와 타당도 검증

신승화,김수현,Shin, Seunghwa,Kim, Su Hyun 중소기업융합학회 2020 융합정보논문지 Vol.10 No.11

본 연구는 한국어판 피츠버그 수면의 질 지수(PSQI-K) 도구의 신뢰도와 타당도를 검증하고 수면장애군의 최적 절단점을 탐색하기 위한 목적으로 수행되었다. 초기 성인 64명을 대상으로 PSQI-K와 Fitbit charge 3^TM을 이용하여 주관적 수면의 질과 수면양상을 측정하였다. 도구의 신뢰도는 Cronbach's alpha coefficient 계수로, 도구의 타당도는 요인분석 및 PSQI-K 점수와 Fitbit tracker 간의 Spearman's rho 상관계수로 분석하였다. 연구결과, PSQI-K 점수의 Cronbach's alpha coefficient는 0.69였고, PSQI-K와 Fitbit tracker의 각 지표값과의 correlation은 0.67 이상으로 유의하였다. 수면장애군 구분을 위한 절단점은 ≥6일 때 민감도 0.93, 특이도 0.84로 가장 적합하였다. PSQI-K는 초기 성인의 수면장애군을 스크리닝함에 있어서 신뢰도와 타당도가 양호한 도구임이 확인되었다. The purpose of the study was to test the reliability and validity of the Korean version of Pittsburgh Sleep Quality Index (PSQI-K) and to examine the cutoff point of the PSQI-K for screening insomnia among young adults. The subjective quality and patterns of sleep in 64 participants were measured using PSQI-K and Fitbit Charge 3^TM. The properties of the instrument were analyzed using Cronbach's alpha coefficient for the internal consistency reliability, factor analysis for the construct validity, and Spearman's rho correlation coefficient for the correlation between the PSQI-K total scores and Fitbit tracker scores. The Cronbach's alpha coefficient of the PSQI-K was 0.69. The Spearman's rho correlation coefficient between the PSQI-K and Fitbit tracker was higher than 0.67. A cutoff point of ≥6.0 had a sensitivity of 0.93 and a specificity of 0.84. In conclusion, the PSQI-K is a reliable and valid instrument to evaluate sleep disturbance in Korean young adults.

병원 간호사의 연속 야간 교대근무와 근무시간이 불면증에 미치는 영향

신승화,김수현 한국직업건강간호학회 2020 한국직업건강간호학회지 Vol.29 No.2

Purpose: This study aimed to identify the influence of consecutive night-shift work and working time on insomnia among hospital nurses. Methods: A descriptive correlational research designutilizing secondary data analysis was adopted. Data on consecutive night-shift work, working time, and insomnia were collected from 64 hospital nurses using a Fitbit activity tracker and questionnaires, and analyzed using hierarchical logistic regression. Results: Consecutive night-shift work for more than three days had a significant influence on insomnia among hospital nurses. Weekly working hours also accounted significantly for the variance in insomnia, exceeding the influence of consecutive night-shift work. Conclusion: Development and implementation of proper schedules to control con- secutive night-shift work and working time is important to alleviate insomnia among hospital nurses.

향기요법이 상하지 수술 환자의 수술 전 불안에 미치는 효과

신승화,이은주,곽미경 국제문화기술진흥원 2018 The Journal of the Convergence on Culture Technolo Vol.4 No.2

본 연구는 근골격계 수술을 앞두고 있는 환자를 대상으로 라벤더를 이용한 향기흡입이 수술전 불안 감소에 미 치는 효과를 규명하기 위해 시행되었다. 연구설계는 비동등성 대조군 전-후 설계의 유사실험연구(Quasi-experimental design)이다. 연구의 대상은 척추마취나 부분마취로 근골격계 수술을 받는 대상자 60명으로 실험군 30명 대조군 30명 이었다. 연구도구는 활력징후 중 혈압과 맥박, 수술 전 불안 자가평가 도구 APAIS(Amsterdam preoperative anxiety information scale)이다. 자료분석은 PASW(SPSS)20..0을 이용하여 실험군과 대조군간의 동질성 검증을 t-test, ²검 증, Fisher's exact test, paird t-test를 이용하여 분석하였다. 연구결과에서 라벤더 향기흡입이 근골격계 수술환자의 수술전 불안을 감소시키고, 향기요법 시행 후에 수축기 혈압과 이완기 혈압의 유의미한 감소를 보이는 결과를 나타냈 다. 이러한 결과는 수술을 앞둔 환자에게 비약물적 중재를 통해 불안의 감소 및 혈압과 맥박을 안정화시키기 위한 간 호전략으로 향기요법을 사용할 수 있을 것이라 사료된다. The study was done to analyze the effects of aroma inhalation method on preoperative anxiety of upper and lower surgical patients. The research design was a nonequivalent control group non-synchronized design. The subjects were a total 60 patients of each group 30 patients that were operated on under general anesthesia for upper and lower limbs surgery. The tool of the Amsterdam preoperative anxiety information scale(APAIS), systolic and diastolic blood pressure, and pulse rate levels was measured the day before surgery. The data were analyzed by the χ2 test Fisher's exact test, paird t-test, and the independent t-test using SPSS 20.0. Study result indicated that Lavender aroma therapy had the effect on reduction of anxiety before surgery and reduction of blood pressure, and pulse rate levels. Therefore, the study result could be used as a scientifical data that can be applied to the nursing interventions that use the aroma inhalation method and contributing to development of the holistic nursing care.

만성질환자의 집단효능감 개념분석

신승화,김수현 경북대학교 간호혁신연구소 2013 경북간호과학지 Vol.17 No.2

Purpose: This study was conducted to investigate the clear concept of the collective efficacy and precenting the clear definition based on the analysis of the meaning and properties of the collective efficacy. Methods: Research was conducted using the Walker and Avant's(2005) conceptual analysis process. Results: The result of this study indicates that 1)desire, 2)shared belief, 3)cohension and 4)task interdependence are attributes of the collective efficacy. It was verified that desire was a confidence in a certain level of achievement for a specific task, shared belief was mind which believe firmly the ability to organize and execute actions required for project achievement, cohension was an intention for the group members to remain in their own group and the degree of loyalty towards the group, task interdependence signified a degree of dependence on the efforts or skills of others who were different from an individual's task performance by the structural characteristics of task. The collective efficacy was found to improve the achievement of organization. Conclusion: The exact understanding of the elements of collective efficacy in nursing area can devise an intervention to enhance the collective efficay as well as self-help groups in the subject of chronic diseases, it can be applied to a variety of nursing areas.

섬유강화 복합재료의 계면평가에 관하여

신승화 부산수산대학교 1991 (最高經營管理者課程)論文集 Vol.1991 No.5

유방암 경험 간호사의 직장 복귀 경험

신승화,김수현 경북대학교 간호혁신연구소 2014 경북간호과학지 Vol.18 No.1

Purpose: The purpose of this study was to understand return-to-work experience of nurses who had suffered from breast cancer. Method: Data were collected using a in-depth interview with open-ended questions from November to December in 2013. The participants were three nurses who had suffered from breast cancer and were working as hospital staff nurses. Each interview was conducted for 90 minutes on average and the transcribed data was analyzed with phenomenological method. Result: By using of Colazzi's method, the data was classified under four themes; 'sensitiveness to changing appearance', 'sensitiveness to surrounding people', 'enthusiastic acceptance of reality,' and gratitude to social support'. As a result, it was verified that the nurses who had suffered from breast cancer tried to help patients by successful returning and working when they came return to hospital after they had experienced suffering from breast cancer in terms of physical, psychological and social aspects. Conclusion: This study will provide a deep understanding about nurses to return to hospital after experience of breast cancer.

시설 입원 노인의 건강지각, 우울 및 자아존중감과의 관계연구

신승화 경북대학교 간호혁신연구소 2011 경북간호과학지 Vol.15 No.2

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